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AbstractRecently, there has been a great interest in the development of innovative wound dressing materials based on natural bioactives, as they can accelerate the healing process and address the issues related to traditional wound dressings. The current study focuses on developing a novel derivative of guar gum (GG) and gallic acid (GA) using a simple, free radical-mediated polymerization reaction aimed at enhancing the antioxidant properties of GG. Multiple spectroscopic investigations were performed to validate the GA–GG conjugate. NMR and FTIR confirmed GA integration, UV spectroscopy indicated changes in electronic transition, DSC analysis suggested a reduction in crystallinity, and XRD revealed structural modifications. SEM revealed a porous structure that reflected its polymerized nature. Due to inadequate mechanical strength and film-forming ability of the synthesized GA–GG conjugate, polyelectrolyte complexation method using chitosan was explored to form a polyelectrolyte complex (PEC) film. The film exhibited a high swelling rate, excellent antioxidant properties, and was both hemocompatible and exhibited improved antimicrobial properties. In vitro, in ovo, and in vivo characterizations were performed to compare the performance of these biocomposite films to those of their counterparts. It promoted angiogenesis in the chick yolk sac membrane and demonstrated good cytocompatibility in cell proliferation studies on the viability of the L929 mouse fibroblast cell line. In vivo wound healing efficacy of the PEC film in wound closure was 94.5% as compared to the untreated disease control group (p < 0.001). This work highlights the development of an innovative GA–GG conjugate/chitosan PEC-based film with significant potential for wound healing applications. [ABSTRACT FROM AUTHOR]