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Background: The aim of this research was to determine the functional state of regional blood flow in young people with varying degrees of anatomical and functional disorders of periodontal tissues. Methods and Results: One hundred and thirty-five young patients with varying degrees of anatomical and functional disorders of periodontal tissues (AFDP) and 52 controls with intact periodontium were examined. All patients with AFDP were divided into three groups: Group 1 included 33 patients in the compensation stage (low degree of periodontal risk, intact periodontium, no clinical symptoms, pale pink gums, no bleeding on probing, dentogingival attachment is not disturbed); Group 2 included 38 patients in the sub-compensation stage (average degree of periodontal risk, disorders of the mucogingival complex, without clinical symptoms); and 64 patients in the decompensation stage (high degree of periodontal risk, disorders of the mucogingival complex, the presence of clinical symptoms, individual or combined signs of periodontal pathology, gingival recession (Class I and II according to Miller's classification). We studied the regional blood flow of periodontal tissues, which was assessed by an ultrasound Doppler graph in a non-invasive way. The parameters of linear (PSV, peak systolic velocity) and volumetric (Qs, maximum systolic volume velocity) velocities of blood flow, as well as the parameters of the wall vascularization (PI, pulsation index; RI, resistance index). Results: The PSV increased statistically significantly with a load compared to rest in the control and sub-compensation groups and decreased in the decompensation group with a load, compared to the rest condition. The PSV increased relative to the control in the subcompensation and decompensation groups in rest and under load. The Qs indicator showed a similar trend in the groups. Qs significantly increased under load, compared to the rest condition in the control, compensation, and subcompensation groups, and decreased in the decompensation group. The Qs values, in comparison with the control, increased in the subcompensation and decompensation groups in the rest condition and under load. The PI increased with the load relative to the rest condition in the control, compensation, and decompensation group. At the same time, PI decreased under the load, compared to the rest condition, in the subcompensation group. In comparison with the control, the PI values decreased in the subcompensation and decompensation groups in the rest condition and under load. The RI significantly changed only in the subcompensation group under load, compared to the rest condition. Only RI values decreased significantly in the subcompensation group under load, compared to the control. Conclusion: The use of Doppler ultrasonography in assessing the state of regional blood flow of periodontal tissues is a highly informative and non-invasive method. The velocity characteristics (linear and volumetric velocities) of blood flow are important diagnostic indicators, allowing the most rapid and reliable assessment of the degree of anatomical and functional disorders of periodontal tissues.

The aim of the study was to establish the qualitative and quantitative composition of microbial flora of dental cavities (DCs) after traditional and alternative preparation, including different methods of isolating the working field. Material and Methods: Our study included 60 patients (mean age of 25.0±3.1 years) with DC Class 1 (Black's classification) without concomitant somatic pathology. To accomplish the study’s aim, 60 teeth were prepared. The main group (MG) consisted of 45 teeth prepared under absolute isolation with a rubber dam (RD). In the MG, 15 teeth were treated traditionally with a diamond bur with red and yellow markings (MG-1), 15 teeth were treated by ultrasound with a diamond tip (MG-2), and 15 teeth underwent hydrokinetic preparation (MG-3) with the Aquacut device (Velopex). In the comparison group (CG), which included 15 teeth, DCs were treated traditionally with a dental bur without the RD. The MG and CG were comparable in terms of the initial state of dental and microbiological status. The study of the qualitative and quantitative composition of the DC microflora showed that all DCs contained pathogenic β-hemolytic streptococcus in the CG. At the same time, the maximum number of cases (80%) was moderately contaminated.. MG-1 and MG-2, as in the CG, were characterized by the predominance of β-hemolytic streptococcus at the bottom of the treated cavity. At the same time, the incidence of moderate contamination decreased by 4 times and single contamination increased in the cultures to 80%, compared to the CG (P=0.001). In MG-3, β-hemolytic streptococcus also dominated in the bottom of DCs after RD setting. The number of colonies was single (66.7% of cases) and moderate (33.3% of cases), indicating a significant increase in single and a decrease in moderate infestation, compared to the CG (P