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Iron(ii) phthalocyaninatoclathrochelates functionalized with terminal reactive formyl group were prepared. Their post-synthetic functionalization gave those with vector pharmacophoric fragment.

The article is devoted to the development of financial monitoring in the public sector. The purpose of this work is to study the essence of the concept of financial monitoring in the budget sphere, and to propose measures to improve it. To achieve this goal, the author reveals the methodological support of financial management in the budget sphere through the study of financial indicators used by public sector organizations in the organization of accounting and budget reporting taking into account the stages of the budget process and the most common budget risks. The concept of “financial monitoring” is considered in various aspects, and the author’s concept of financial monitoring in the budget sphere in broad and narrow senses is given. Due to the lack of a legal definition of financial monitoring in the budget sphere and the lack of scientific approaches, an attempt is made to study the issues of its essence as a scientific category. Based on the analysis of financial monitoring in the budget sphere for 2019 (on the example of the Ministry of Internal Affairs of Russia), the directions for improving the studied sphere are proposed with their systematization by stages of the budget process taking into account the risk-oriented approach. The article considers the contents of the financial monitoring in the public sector expressed in the observation, analysis and evaluation, timely response to ongoing subjects of financial monitoring activity in terms of the execution of budget authority, including: income forming (administration), distribution (redistribution) and use of financial, material and other public resources; assessment of the effectiveness of management decisions being made and have already been made regarding financial management, completeness, reliability and timeliness of accounting and budget reporting; prevention of violations in the budget sphere. The most important goals of financial monitoring in the budget sphere are emphasized: continuous systematic monitoring of the implementation of decisions on the administration of revenues and execution of budget expenditures, timely correction of existing problems in financial management in the budget sphere and development of proposals for their optimization within a single socio-economic system by applying a risk-based approach. The research shows that an effective system of financial monitoring in the budget sphere as one of the types of financial control is a guarantee of increasing the efficiency of budget revenue generation, use (distribution and / or redistribution) of public finances, ensuring transparency of the budget process at all stages of its implementation, as well as preventing corruption and other abuses in the budget sphere. The results of the research can be used by specialists in the field of financial management in the budget sphere in practical activities, as well as in the educational activities of higher education institutions – in the theoretical part.

The paper presents a mathematical model of CH4 recovery from gas hydrate deposits via injection of liquid CO2. The process of CH4 recovery is supposed to occur on the moving frontal boundary separating the deposit into two domains. The near domain is saturated with liquid CO2 and its hydrate while the far one is saturated with CH4 and its hydrate. Self-similar solutions describing the temperature and pressure fields were obtained. The effect of main parameters on the dynamics of the process under study was explored. It was revealed that at low temperatures of the CO2 being injected the temperature in the far domain did not exceed that of equilibrium for decomposition of CH4 gas hydrate. It was also found that with increasing pressure at which CO2 was injected into the gas hydrate do-main, the extension of the region saturated with CO2 hydrate was increasing.

The article analyzes theoretical (organizational) and practical aspects of educational technology to enhance the quality of graduates’ education and their competitiveness on the labour market. The analysis of the status of hospitality industry and its domestic and international trends under current economic conditions has predestined the need to improve the role of educational institutions as human resources training, and the enterprises of industry - to create the conditions for the adaptation and development of the competitive capacity of specialists. The authors emphasize the advisability of introducing a public professional examination of education programs, and starting July 01, 2016 - procedures of confirmation the education quality of each university graduate. The article also defines the role of the professional community representatives in the educational programs formation, their accreditation, which indicates the need for education and business system. The possible directions of policy of educational and entrepreneurial system of hospitality industry are formulated, criteria for graduates’ competitiveness estimation by a number of components of general educational, practical and social activities are offered. The authors present teaching experience of interdisciplinary design programs using Business English in teaching university students as a development element of business resource of graduates. The indicators for student activities estimation and integrated factor of graduate’s competitiveness are offered. This allows protecting a document of high education in front of professional community.

Objective Muscle unloading leads to its atrophy development. The MuRF-1 and MAFbx E3-ligases expression is increasing under this condition. FOXO3 was considered to be the only transcription factor that triggers E3-ligases expression. Beharry A.W. et al pinpoints HDAC1 as a primary regulator of FoxO in skeletal muscle that is both sufficient and required for skeletal muscle atrophy. We aimed to determine the role of histone deacetylase 1 (HDAC1) proteins in activation of MuRF-1 and MAFbx E3-ligases expression at the early stage of muscle unloading. Methods We investigate it by CI-994 (inhibitor of HDAC1) administration in male Wistar rats (180-200 g) upon 3-day hindlimb suspension. The method of hindlimb suspension was described in Morey-Holton E & Globus R (2002). 24 animals were divided into 3 groups (n=8 in each): C-control, CI - hindlimb suspension with CI-994 (i.p. 1 mg/kg/day), or placebo (HS group) administration. The animals were anaesthetized with an i.p. injection of tribromoethanol (240 mg/kg), soleus muscles were surgically excised, frozen in liquid nitrogen. The Western blot and RT-PCR analyzes were done. The statistical analysis was performed using REST 2009 v.2.0.12 and Bio-Rad CFX Manager programs at the significance level set at 0.05. The significant differences between groups were statistically analyzed using Mann-Whitney test. Results The evaluation of the levels of mRNA expression of MuRF-1 and MAFbx showed that CI-994 treatment inhibited unloading induced up-regulation of MAFbx in CI group but had no effect on mRNA expression of MuRF-1. After unloading, mRNA expression of MAFbx increased 2.12-fold (p < 0.05) in HS group. There were statistically significant differences in MAFbx mRNA expression between HS and CI groups. When compared with the control, unloading increased MuRF-1 mRNA expression 1.67- and 1.56-fold in HS and CI groups, respectively. CI-994 treatment also inhibited unloading-induced upregulation of mRNA expression of ubiquitin. The levels of ubiquitin mRNA expression when compared with the control were 4.21- and 2.32-fold in HS and CI groups, respectively. We did not find any differences in the content of phosphorylated anabolic signaling system components (Akt/mTOR/S6k) between both suspended groups (CI and HS). Conclusions Therefore, HDAC1 inhibiting prevented hindlimb suspension-induced up-regulation of MAFbx and ubiquitin, but did not any effect MuRF-1expression. This work was supported by Russian Science Foundation (grant № 18-15-00062).

Objective Unloading causes rapid skeletal muscle atrophy mainly due to the increased protein degradation. Muscle proteolysis results from the activation of ubiquitin-proteasome systems. The ubiquitination proteins are carried out by muscle-specific E3 ubiquitin ligases – MuRF-1 and MAFbx. It is known that MuRF-1 and MAFbx expression significantly increases on the third day of muscle unloading. We tested the hypothesis that p38 MAPK participates in the regulation of E3 ligases expression and the development of skeletal muscle atrophy during unloading. To check this idea we inhibited p38 MAPK by VX-745. Methods 21 male Wistar rats were divided into 3 groups (7 rats in each group): intact control (C), rats suspended for 3 days (HS) and rats suspended and injected i.p. with VX-745 (10 mg/kg/day) (VX). The hindlimb suspension was carried out according to Morey-Holton technique. The animals were anaesthetised with an i.p. injection of tribromoethanol (240 mg/kg). Under anesthesia, the m.soleus were excised, frozen in liquid nitrogen, and stored at -80°C until further analysis. All procedures with the animals were approved by the Biomedicine Ethics Committee of the Institute of Biomedical Problems of the Russian Academy of Sciences/Physiology section of the Russian Bioethics Committee. The statistical analysis was performed using the REST 2009 v.2.0.12 and Origin Pro programs at the significance level set at 0,05. The results are given as median in percent and interquartile range (0.25-0.75). Results The muscle weight in HS group was significantly reduced (72,3±2,5 mg) compared to C (83,0±3 mg), p

Due to the increasing average age of skilled professionals in the field of construction and municipal facilities as well as increasing the number of migrants who often do not possess the necessary skills to carry out construction work and research, there is an urgent need to an influx of skilled workers with different levels of education and training. In this regard, it is necessary to improve the skills of teachers and trainers, to help them in their acquisition of professional experience in work with the modern technological equipment, contribute to the development of innovative technologies applied to the leading construction companies in the region; and train the concept of “double teacher”, who can solve several problems. Solving this problem allows Technical College will contribute effective organization of production practices, will increase interest in the building trade, the formation of the graduates of the college corporate spirit and the emergence of confidence in their own employability.

The development of methodical recommendations on improvement of marketing and logistic component of competitiveness management mechanism, justification of a business system of management for enterprise association in the industry and services sector are considered in this paper.

A novel species is proposed for two facultatively methanotrophic representatives of the genus Methylocystis , strains H2sT and S284, which were isolated from an acidic (pH 4.3) Sphagnum peat-bog lake (Teufelssee, Germany) and an acidic (pH 3.8) peat bog (European North Russia), respectively. Cells of strains H2sT and S284 are aerobic, Gram-negative, non-motile, curved coccoids or short rods that contain an intracytoplasmic membrane system typical of type-II methanotrophs. They possess both a soluble and a particulate methane monooxygenase (MMO); the latter is represented by two isozymes, pMMO1 and pMMO2. The preferred growth substrates are methane and methanol. In the absence of C1 substrates, however, these methanotrophs are capable of slow growth on acetate. Atmospheric nitrogen is fixed by means of an aerotolerant nitrogenase. Strains H2sT and S284 grow between pH 4.2 and 7.6 (optimum pH 6.0–6.5) and at 8–37 °C (optimum 25–30 °C). The major fatty acids are C18 : 1ω8c, C18 : 1ω7c and C16 : 1ω7c; the major quinone is Q-8. The DNA G+C content is 62.0–62.3 mol%. Strains H2sT and S284 share identical 16S rRNA gene sequences, which displayed 96.6–97.3 % similarity to sequences of other taxonomically characterized members of the genus Methylocystis . Therefore, strains H2sT and S284 are classified as members of a novel species, for which the name Methylocystis bryophila sp. nov. is proposed; strain H2sT ( = DSM 21852T = VKM B-2545T) is the type strain.

BACKGROUND: The house dust mites (HDM) Dermatophagoides farinae and D. pteronyssinus are important environmental allergens implicated in the pathogenesis of human and canine atopic dermatitis. Sensitization to HDM measured by allergen‐specific IgE is common in Finnish atopic dogs. Studies on HDM prevalence in Finland are few but suggest that HDM are scarce. OBJECTIVE: The aim of the present study was to evaluate the prevalence of HDM in the home environments of atopic dogs in Finland. METHODS: Dust samples were obtained from the homes of 50 atopic dogs. Samples were collected by vacuuming the owners’ mattresses and each dog's bed. In each case, an area of 21 × 30 cm was vacuumed for 2 min. Samples weighing 100 mg or more were considered sufficient for determination of HDM allergen concentrations (Der f 1 and Der p 1) using standardized ELISA. Samples sufficient for further analysis were also examined by direct microscopy for the presence of mites and by multiplex PCR for HDM DNA. RESULTS: Eighty one samples were sufficient for analysis by ELISA, 59 by PCR and 29 by direct microscopy. A single sample was analysed from four homes in which the dog shared the owner's bed. Der f 1 was detected in three samples (3.7%). Der p 1 was not detected in any sample. No mites were identified on microscopy. Five samples were positive for HDM on multiplex PCR (8.4%). CONCLUSION: House dust mites seem to be uncommon in the home environment of atopic dogs in Finland despite reported frequent allergen‐specific IgE antibodies.

In the article authors raise the actual direction — food services workers of industrial enterprises. Provision of food should be produced in integrating efforts of resources and the possibilities of partners (industry — trade) on the basis of the organizational and economic model of food production system.

Hypersensitivity dermatitides (HD) are commonly seen in cats, and they are usually caused by environmental, food and/or flea allergens. Affected cats normally present with one of the following clinical reaction patterns: head and neck excoriations, usually symmetrical self-induced alopecia, eosinophilic skin lesions or miliary dermatitis. Importantly, none of these clinical presentations is considered to be pathognomonic for HD skin diseases, and the diagnosis of HD is usually based on the exclusion of other pruritic diseases and on a positive response to therapy. The objectives of this study were to propose sets of criteria for the diagnosis of nonflea-induced HD (NFHD). We recruited 501 cats with pruritus and skin lesions and compared clinical parameters between cats with NFHD (encompassing those with nonflea, nonfood HD and those with food HD), flea HD and other pruritic conditions. Using simulated annealing techniques, we established two sets of proposed criteria for the following two different clinical situations: (i) the diagnosis of NFHD in a population of pruritic cats; and (ii) the diagnosis of NFHD after exclusion of cats with flea HD. These criteria sets were associated with good sensitivity and specificity and may be useful for homogeneity of enrolment in clinical trials and to evaluate the probability of diagnosis of NFHD in clinical practice. Finally, these criteria were not useful to differentiate cats with NFHD from those with food HD.

A mathematical model of psychotropic activity has been built based on calculations of the electrostatic potential of molecules of compounds possessing activity of this type; the conclusion has been drawn that this method has general applicability for solving the “structure–property” problem.

Hypersensitivity dermatitides (HD) are commonly seen in cats, and they are usually caused by environmental, food and/or flea allergens. Affected cats normally present with one of the following clinical reaction patterns: head and neck excoriations, usually symmetrical self-induced alopecia, eosinophilic skin lesions or miliary dermatitis. Importantly, none of these clinical presentations is considered to be pathognomonic for HD skin diseases, and the diagnosis of HD is usually based on the exclusion of other pruritic diseases and on a positive response to therapy. The objectives of this study were to propose sets of criteria for the diagnosis of nonflea-induced HD (NFHD). We recruited 501 cats with pruritus and skin lesions and compared clinical parameters between cats with NFHD (encompassing those with nonflea, nonfood HD and those with food HD), flea HD and other pruritic conditions. Using simulated annealing techniques, we established two sets of proposed criteria for the following two different clinical situations: (i) the diagnosis of NFHD in a population of pruritic cats; and (ii) the diagnosis of NFHD after exclusion of cats with flea HD. These criteria sets were associated with good sensitivity and specificity and may be useful for homogeneity of enrolment in clinical trials and to evaluate the probability of diagnosis of NFHD in clinical practice. Finally, these criteria were not useful to differentiate cats with NFHD from those with food HD.