1. Fluorescence labels may significantly affect the protein adsorption on hydrophilic nanomaterials
- Author
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Kaloian Koynov, Katharina Landfester, Svenja Winzen, and Kristin Mohr
- Subjects
Polymers ,Surface Properties ,Serum albumin ,Fluorescence correlation spectroscopy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Nanocapsules ,Colloid and Surface Chemistry ,Adsorption ,Animals ,Humans ,Physical and Theoretical Chemistry ,Serum Albumin ,Fluorescent Dyes ,biology ,Chemistry ,Isothermal titration calorimetry ,Surfaces and Interfaces ,General Medicine ,021001 nanoscience & nanotechnology ,Blood proteins ,Nanostructures ,0104 chemical sciences ,Biochemistry ,biology.protein ,Cattle ,Nanocarriers ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions ,Biotechnology ,Protein adsorption - Abstract
Fluorescently labelled proteins are often used to study processes in vitro, e.g. the binding of proteins to cell surfaces or the adsorption of plasma proteins on drug nanocarriers. However, the fact that the fluorescent labelling may affect the protein properties is frequently neglected. On the example of a simple model system, we reiterate the importance of this issue by showing that even a single label may perturb interactions between hydrophilic starch-based nanocapsules and serum albumin and thus prevent binding.
- Published
- 2016