1. A penicillin and metronidazole resistant Clostridium botulinum strain responsible for an infant botulism case
- Author
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Christelle Mazuet, Eun-Jeong Yoon, Christiane Bouchier, Sophie Boyer, Stéphanie Pignier, Martine Pestel-Caron, Djalal Meziane-Cherif, Clémentine Dumant-Forest, Michel R. Popoff, Christine Legeay, Isabelle Doehring, Patrice Courvalin, Philippe Bouvet, Jean Sautereau, Thierry Blanc, Suzana Quijano-Roy, Bactéries anaérobies et Toxines, Institut Pasteur [Paris], Agents antibactériens, Hôpital Charles Nicolle, Hôpital Charles Nicolle - CHU Rouen, Hôpital Raymond Poincaré, Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Raymond Poincaré - Université Paris Descartes - Paris 5 (UPD5), Génomique (Plate-Forme), Centre de références maladies neuromusculaires, CHU Raymond Poincaré, Sequencing was performed at the GenomicsPlatform, member of 'France Génomique' consortium (ANR10-INBS-09-08).This work was supported by Institut Pasteur, Institut national de Veille Sanitaire, and byan unrestricted grant from Reckitt Benckiser., Groupe de Recherche sur les Antimicrobiens et les Micro-Organismes (GRAM 1.0), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Service de pédiatrie médicale et médecine de l'adolescent [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Service de pédiatrie néonatale et réanimation - neuropédiatrie [CHU Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Hôpital Raymond Poincaré [AP-HP], Génomique (Plate-Forme) - Genomics Platform, Handicap neuromusculaire : Physiopathologie, Biothérapie et Pharmacologies appliquées (END-ICAP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Filière Neuromusculaire (FILNEMUS), Sequencing was performed at the Genomics Platform, member of 'France Génomique' consortium (ANR10-INBS-09-08). This work was supported by Institut Pasteur, Institut national de Veille Sanitaire, and by an unrestricted grant from Reckitt Benckiser., ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Institut Pasteur [Paris] (IP), Hôpital Charles Nicolle [Rouen], and Normandie Université (NU)-Normandie Université (NU)-CHU Rouen
- Subjects
0301 basic medicine ,Microbiology (medical) ,infant botulism ,Botulinum Toxins ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Microbial Sensitivity Tests ,Penicillins ,Biology ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Feces ,Antibiotic resistance ,metronidazole ,Drug Resistance, Bacterial ,Genes, Regulator ,medicine ,Clostridium botulinum ,Humans ,Botulism ,Etest ,botulism ,Infant Botulism ,Infant ,Membrane Transport Proteins ,General Medicine ,Sequence Analysis, DNA ,Penicillinase ,medicine.disease ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Penicillin ,Metronidazole ,Infectious Diseases ,antibiotic-resistance ,penicillin ,Multigene Family ,Female ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Genome, Bacterial ,medicine.drug - Abstract
International audience; An infant botulism clinical course was characterized by several relapses despite therapy with amoxicillin and metronidazole. Botulism was confirmed by identification of botulinum toxin and C. botulinum in stools. A C. botulinum A2 strain resistant to penicillins and with heterogeneous resistance to metronidazole was isolated from stool samples up to 110 days after onset. Antibiotic susceptibility was tested by disk agar diffusion and minimum inhibitory concentrations were determined by Etest. Whole genome sequencing allowed detection of a gene cluster composed of blaCBP for a novel penicillinase, blaI for a regulator, and blaR1 for a membrane bound penicillin receptor in the chromosome of the C. botulinum isolate. The purified recombinant penicillinase was assayed. Resistance to β-lactams was in agreement with the kinetic parameters of the enzyme. In addition, the β-lactamase gene cluster was found in three C. botulinum genomes in databanks and in two out of 62 genomes of our collection, all the strains belonging to group I C. botulinum. This is the first report of a C. botulinum isolate resistant to penicillins. This stresses the importance of antibiotic susceptibility testing for adequate therapy of botulism.
- Published
- 2016
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