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Osteosarcoma is one of the most aggressive primary malignant bone tumors, and the effect of first-line therapeutic schedule had no improvement in past few years. Therefore, it is extremely urgent to develop new drugs against osteosarcoma. In this study, human osteosarcoma U2 OS cells as a model in vitro were treated with LY404039 and dimethyl sulfoxide. CCK-8 proliferation test, transwell assay, and flow cytometry were used to assess cell proliferation, invasion/migration, and apoptosis, respectively. Moreover, western blotting was performed to determine the relative expression of protein related to apoptosis and PI3K signaling pathway. The proliferation of U2 OS cells was significantly inhibited 72 h after LY404039-treatment by CCK-8 proliferation test. Matrigel invasion analysis showed the numbers of invasive cells were significantly decreased in LY404039-treated group compared with the NC group. Furthermore, LY404039 led to the remarkable reduction of the number of U2 OS cells passing through the microwells of the transwell chamber. The expression levels of anti-apoptotic protein BCL-2 decreased, and the expression of pro-apoptotic proteins Active Caspase3, Bax increased concurrently after LY404039 stimulation using western blot. The phosphorylation of AKT and mTOR were significantly inhibited in U2 OS cells treated with LY404039. Also, the activity of the downstream proteins such as p70S6K and Cyclin D1 were also significantly decreased. LY404039 could inhibit proliferation and metastasis of osteosarcoma cells, which might be attributed to the enhancement of apoptosis via PI3K pathway.

This paper will introduce the Hilbert-Huang Transform for Analysis of Universal blind steganalysis detection task. Firstly, This method use its efficient unsteady-state modeling ability to constucte features of steganography object and adopt independent variable analysis method to extract and refine feature. In the classification stage, by constructing the compound ANOVN kernel function of the Steganalysis field to improve data processing capabilities of nonlinear.

For the characteristics that the signing speed must be quick and the system must be simply deployed in the digital signature system of office automation, a method of digital signature with encryption based on combined symmetric key, symmetric technology and hardware technology is proposed. This method stores key seed matrix, symmetric key algorithm and combined symmetric key algorithm in hardware equipment, in additional to, hardware equipment has the functions of creating symmetric key by combined key seed matrix with combined symmetric key algorithm and digital signature. The advantages of the proposed method is that the key is one-time and time-variant and the key update and maintenance is automation so the key is maintenance-free; Compared with traditional Asymmetric digital signature algorithms, this method has the superiority of fast deciphering and simple key management.

In order to describe the user's profile exactly and provide what they need in information filtering, a new method to weight the user's characterized demand and filter for him is put forward. In the paper, at first we define the terms' weight of the user's profile according to the order of the proposed sequence, then calculate the similarity between the user's profile and the documents using the semantic dictionaries of Synonym Dictionary and HowNet, and at last submit the results to the user sorted in descending order of the similarity. The experiments show that it can improve the precision of information filtering.