8 results on '"Suran Huang"'
Search Results
2. KCNH3 Predicts Poor Prognosis and Promotes Progression in Ovarian Cancer
- Author
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Zhongjun Li, Bin Zhang, Suran Huang, Lishan Huang, Chuangyu Wen, Shulin Zhong, Yijing Ou, and Li Wei
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0301 basic medicine ,medicine.diagnostic_test ,Cell growth ,Cancer ,Biology ,Cell cycle ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Western blot ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,Immunohistochemistry ,Gene silencing ,Pharmacology (medical) ,Ovarian cancer - Abstract
Background Ovarian cancer (OC) is one of the most common causes of cancer-related death among women; accordingly, new biomarkers of OC are urgently needed. Potassium voltage-gated channel sub-family H member 3 (KCNH3) is a voltage-gated potassium channel member involved in cognitive function and diabetes. Here, we aimed to elucidate the role and potential molecular mechanisms of KCNH3 in OC. Materials and methods KCNH3 expression levels in OC tissues were analyzed using TCGA data and confirmed by RT-qPCR and immunohistochemistry in OC tissues. The cell counting kit-8 was used to assess cell proliferation in OC cells in which KCNH3 was knocked-down with small interference RNA (siRNA). Wound-healing and transwell invasion assays were used to assess migratory and invasive abilities, respectively. Cell cycle distribution and apoptosis were determined using a flow cytometer. Gene set enrichment analysis and Western blot were used to investigate the potential pathways of KCNH3 in OC development. Results TCGA data and RT-qPCR results from patients with OC revealed high KCNH3 expression in OC tissues compared to normal ovarian tissues. Survival analysis in patients with OC suggested that high KCNH3 expression might be an independent predictor for poor overall survival and disease-free survival. In vitro studies showed that KCNH3 silencing in OC cells could inhibit cell proliferation and migration ability, and induce apoptosis and G2/M phase arrest. Furthermore, Western blot results showed that KCNH3 silencing might induce downregulation of RPA1 and RPA2 expression level in both SKOV3 and COC1 cells. Conclusion KCNH3 plays an important role in cancer progression in patients with OC. Further investigation might reveal KCNH3 as a potential biomarker for prognosis or diagnosis in OC.
- Published
- 2020
3. In Utero Exposure to Fine Particles Decreases Early Birth Weight of Rat Offspring and TLR4/NF-κB Expression in Lungs
- Author
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Zhongjun Li, Chuangyu Wen, Wenting Tang, Yaoguang Huang, Wen Sun, Dunjin Chen, Suran Huang, Lili Du, and Zhiqiang Yu
- Subjects
Litter (animal) ,Male ,Offspring ,Birth weight ,Toxicology ,complex mixtures ,Andrology ,Rats, Sprague-Dawley ,Pregnancy ,Medicine ,Animals ,Birth Weight ,Respiratory system ,Particle Size ,Lung ,chemistry.chemical_classification ,Reactive oxygen species ,Air Pollutants ,business.industry ,NF-kappa B ,General Medicine ,Rats ,Toll-Like Receptor 4 ,Low birth weight ,medicine.anatomical_structure ,chemistry ,Animals, Newborn ,In utero ,Female ,medicine.symptom ,business ,Injections, Intraperitoneal - Abstract
Particulate matter (PM2.5) exposure is reported to have deleterious effects on health. Maternal PM2.5 exposure has been confirmed to damage the growth of somatic cells and enhance the incidence of chronic respiratory diseases in children. Here we aim to investigate the impact of in utero PM2.5 exposure on early birth weight and postnatal lung development. Pregnant Sprague-Dawley rats were administered PM2.5 (0.1, 0.5, 2.5, or 7.5 mg/kg) intraperitoneally every 3 days until birth. Maternal and birth outcomes and somatic growth were monitored. Lungs were collected on PND1 (where PND = postnatal day) and PND28; the lung wet-to-dry weight ratio (W/D) was analyzed, and reactive oxygen species (ROS) levels were measured. Expression of Toll-like receptor 4 (TLR4) and NF-κB were evaluated by Western blotting and quantitative RT-PCR. There were no significant intergroup differences for maternal outcomes; however, offspring exposed in utero to 2.5 and 7.5 mg/kg PM2.5 were significantly smaller in litter weight than the controls. In utero exposure to 2.5 and 7.5 mg/kg PM2.5 led to lower body weight after birth and disrupted lung development during infancy. ROS levels were significantly increased in the 7.5 mg/kg PM2.5 group. PM2.5-treated rats showed upregulated pulmonary expression of TLR4 and NF-κB. Maternal PM2.5 exposure enhances the risk of low birth weight and affects lung alveolar development. The underlying molecular mechanisms may involve TLR4/NF-κB signaling.
- Published
- 2021
4. Claudin 10 acts as a novel biomarker for the prognosis of patients with ovarian cancer
- Author
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Wenting Xuan, Niankun Chen, Chuangyu Wen, Biyan Lu, Zhiyong Hou, Zhongjun Li, Suran Huang, and Lishan Huang
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Metastasis ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,claudin 10 ,Medicine ,competing endogenous RNA ,Survival rate ,Oncogene ,business.industry ,Cancer ,Articles ,Nomogram ,medicine.disease ,signaling pathways ,Biomarker (cell) ,ovarian cancer ,030104 developmental biology ,030220 oncology & carcinogenesis ,prognosis ,business ,Ovarian cancer - Abstract
Ovarian cancer (OC) is one of the most fatal gynecological malignancies in the world and confers a poor 5-year survival rate. The present study was designed to discover novel prognostic markers for patients with OC in order to estimate disease metastasis or recurrence. Based on the large cohorts of transcriptome data from multicenter sources, a comprehensive analysis was performed to explore potential prognostic markers. A total of 269 differentially expressed genes were identified, of which 32 were upregulated and 237 downregulated in OC tissues compared with the corresponding expression in normal tissues. Kaplan-Meier analysis, log-rank test and nomogram analysis were employed to demonstrate that low expression levels of claudin 10 (CLDN10) were associated with a less favorable disease prognosis. The most promising prognostic marker for OC was subsequently selected. Additionally, the prognostic nomogram was constructed in order to assess the 5-year survival rate using CLDN10 expression as a prognostic marker for OC. Furthermore, gene set enrichment analysis and analysis of the tumor-associated competing endogenous RNA network were performed to elucidate the potential biological processes associated with CLDN10 expression. The current results indicated that CLDN10 may influence OC progression via transforming growth factor-β (TGF-β)- or WNT/β-catenin-induced epithelial-to-mesenchymal transition (EMT). The associations among CLDN10, microRNA-486-5p, TGF-β, WNT/β-catenin and EMT should be further investigated in future studies.
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- 2020
5. Chaetocin induces caspase‑dependent apoptosis in ovarian cancer cells via the generation of reactive oxygen species
- Author
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Zhongjun Li, Zhiyong Hou, Suran Huang, Li Wei, Lishan Huang, and Weibiao Ye
- Subjects
0301 basic medicine ,Cancer Research ,caspase ,Cell ,chaetocin ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Cytotoxic T cell ,Viability assay ,Caspase ,reactive oxygen species ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Cell growth ,apoptosis ,Articles ,Cell cycle ,ovarian cancer ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,chemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein - Abstract
Ovarian cancer (OC) is one of the most common types of cancer among women worldwide. The majority of patients with OC respond to current chemotherapy approaches initially; however, patients are likely to experience cancer recurrence and become resistant to the chemotherapy. Therefore, novel agents for the treatment of OC are urgently required. Chaetocin, a natural product isolated from Chaetomium fungi, has been reported to exhibit anticancer activity against various types of cancer; however, the pharmacological action and detailed mechanism underlying the effects of chaetocin on OC cells remain unclear. Therefore, the present study investigated the cytotoxic effects of chaetocin on OC cells. A Cell Counting kit-8 assay was used to study cell viability, a colony formation assay was used to assess cell proliferation, flow cytometry was used to detect apoptosis, cell cycle and reactive oxygen species (ROS) generation, and western blotting was used to determine the protein levels of poly (ADP-ribose) polymerase, caspase-3 and cleaved-caspase-3. The results demonstrated that chaetocin significantly decreased the viability of OC cells. Chaetocin inhibited the proliferation and induced G2/M phase arrest of the OVCAR-3 OC cell line. Additionally, chaetocin induced apoptotic cell death in OVCAR-3 cells via the caspase pathway. It was observed that chaetocin induced the accumulation of ROS in OVCAR-3 cells. Treatment with the ROS scavenger N-acetyl-L-cysteine reversed the apoptotic effects and activation of the caspase pathway induced by chaetocin. Collectively, these results revealed that chaetocin suppressed the proliferation and promoted the caspase-dependent apoptosis of OC cells by increasing the levels of ROS. Therefore, chaetocin may serve as a potential therapeutic agent for the treatment of OC.
- Published
- 2019
6. Concurrent Genetic and Standard Screening for Hearing Impairment in 9317 Southern Chinese Newborns
- Author
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Qi Peng, Suran Huang, Wenrui Li, Baimao Zhong, Yuan Liang, Lin Yang, Qiang Ma, Liu Qian, Siping Li, Xiaomei Lu, and Keze Ma
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Male ,China ,Pediatrics ,medicine.medical_specialty ,Hearing loss ,Genetic counseling ,MEDLINE ,Susceptibility gene ,Audiology ,Connexins ,Hearing screening ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,otorhinolaryngologic diseases ,medicine ,Humans ,Genetic Predisposition to Disease ,Genetic Testing ,Hearing Loss ,030223 otorhinolaryngology ,Genetics (clinical) ,business.industry ,Infant, Newborn ,Southern chinese ,Membrane Transport Proteins ,General Medicine ,Connexin 26 ,Clinical trial ,Sulfate Transporters ,Female ,Detection rate ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
The goal of this study was to investigate the use of concurrent genetic screening together with standard newborn hearing screening (NHS) in an effort to provide a scientific basis for the beneficial use of concurrent genetic hearing screening in newborns. Our aim was to improve the neonatal detection rate of hearing impairment and the potential for hearing loss, allowing for increased early intervention and potentially allowing for prevention of later onset hearing loss. This information could also be used to increase the effectiveness of genetic counseling regarding hearing impairment.A total of 9317 neonates from Children's Hospital of Dongguan and Dongguan People's Hospital were included in this study between January 2015 and October 2015. Twenty hotspot hearing-associated mutations of four common deafness- susceptibility genes (GJB2, GJB3, SLC26A4, and MTRNR1) were analyzed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results of genetic screening and NHS were concurrently analyzed.A total of 129 infants (1.38%) exhibited hearing loss as determined by otoacoustic emission (OAE) testing. The genetic screening revealed that 348 (3.74%) individuals had at least one mutant allele. In total, 34 (0.36%) of the neonates carried a causal complement of mutations. The overwhelming majority of the genetically referred newborns passed the OAE hearing screening, but could be at risk for later hearing loss.This study furthers the understanding of the etiology of hearing loss and proves that it is beneficial to use genetic screening along with OAE screening of neonates to improve detection rates of at-risk infants. Our results show that this concurrent testing allows for better early identification of infants at risk for hearing loss, which may occur before speech and language development. Prevention of hearing loss can be achieved by avoiding the use of antibiotics containing amino glycosides in infants whose mutations make them extremely sensitive to these antibiotics. This information is also useful in genetic counseling, providing region-specific mutation information.
- Published
- 2016
7. Expression of HMGB1 in maternal exposure to fine particulate air pollution induces lung injury in rat offspring assessed with micro-CT
- Author
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Wenting Tang, Zhiqiang Yu, Yanmei Zhou, Wen Sun, Suran Huang, Dunjin Chen, Xiaomei Li, Jingsi Chen, Bolan Yu, Xiuying Li, and Lili Du
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0301 basic medicine ,Offspring ,Inflammation ,Enzyme-Linked Immunosorbent Assay ,Lung injury ,Toxicology ,HMGB1 ,Proinflammatory cytokine ,Andrology ,Rats, Sprague-Dawley ,03 medical and health sciences ,Pregnancy ,Medicine ,Animals ,Respiratory system ,HMGB1 Protein ,Particle Size ,Lung ,biology ,business.industry ,General Medicine ,Lung Injury ,X-Ray Microtomography ,medicine.disease ,Immunohistochemistry ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Maternal Exposure ,Prenatal Exposure Delayed Effects ,biology.protein ,Cytokines ,Female ,Particulate Matter ,medicine.symptom ,business - Abstract
Objectives Maternal particulate matter with less than 2.5 μm in diameter (PM2.5) is associated with an increased risk for acute lower respiratory infections and allergic airway inflammation; however, its effect on the developing lung remains unclear. The aim of this study is to determine the effect of maternal PM2.5 during pregnancy on lung development in offspring. Methods Timed pregnant Sprague-Dawley rats were treated with PM2.5 (0.1, 0.5, 2.5, or 7.5 mg/kg) once every 3 days from day 0–18 of pregnancy and delivered at term. Lungs were obtained on postnatal day 0, the structure of the lung was analyzed by quantitative micro-computed tomography (CT) and the levels of proinflammatory cytokines were analyzed using enzyme-linked immunosorbent assay (ELISA). The expression of high mobility group box-1 (HMGB1) was also detected by immunohistochemistry, Western blotting, and quantitative RT-PCR. Results Ground-glass opacity and high-density volumes in CT slice images of maternal PM2.5-exposure rats were observed. The concentrations of IL-1, IL-6 and TNF-α were significantly increased by 2.36-, 3.91- and 4.36-fold, respectively, in the rats of the PM-7.5 group compared with the rats in the control group. The PM2.5-treated rats showed a significant upregulated expression of HMGB1 in lungs. Conclusions PM2.5 exposure during pregnancy results in lung inflammation in offspring mediated by increased HMGB1 expression, followed by upregulated IL-1, IL-6 and TNF-α secretions, which may contribute to the development of inflammatory lung diseases in later life.
- Published
- 2017
8. Application of QF-PCR Technology Combined With Early Pregnancy Ultrasound in Prenatal Screening for Fetal Chromosomal Aneuploidy.
- Author
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Chan Li, Lijuan Xiao, Li Tang, Daidi Zeng, and Suran Huang
- Subjects
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PRENATAL diagnosis , *ANEUPLOIDY , *POLYMERASE chain reaction , *FETAL ultrasonic imaging , *KARYOTYPES - Abstract
Context • Abnormalities in the meiosis process after sperm-egg union can cause fetal chromosome aneuploidy. The rate of birth defects and the mortality of fetuses with chromosome aneuploidy is significantly higher than that of fetuses with normal chromosomes. Both ultrasound and quantitative fluorescence polymerase chain reaction (QF-PCR) have limitations when used singly, but their combined use may provide better diagnoses. Objective • The study intended to investigate the value of QF-PCR combined with ultrasound in early pregnancy for prenatal screening for fetal chromosomal aneuploidy, to contribute to the improvement of prenatal examinations, ultimately enhancing the early detection and management of the aneuploidies. Design • The research team performed a retrospective study. Setting • The study took place at the Affiliated Dongguan Hospital at Southern Medical University in Dongguan, China. Participants • Participants were 1082 pregnant women who underwent an ultrasound examination in early pregnancy, 11 weeks to 13 + 6 weeks, at the hospital between January 2019 and January 2022. Outcome Measures • Using the results of participants’ ultrasounds and QF-PCR testing, the research team used the gold standard, a chromosomal karyotype analysis, to evaluate the efficacy in diagnosing fetal chromosomal aneuploidies, of ultrasound alone, QF-PCR testing alone, and their combination. Results • Early-pregnancy ultrasound detected 15 abnormalities, of which seven were an abnormal thickness of the transparent layer of the neck, 22 were abnormal nasal bone, four were a fish-scale appearance of bilateral soles of the feet, and three were other abnormalities, such as an incomplete quadrant of the heart, gastroceles, or dilatation of the pelvis. No cases of missed or failed karyotype cultures occurred. The QF-PCR detected 21 abnormal fetuses, including six with trisomy 21 syndrome, two with trisomy 18 syndrome, one with trisomy 13 syndrome, and 11 with sex-chromosome abnormalities. The sensitivity, specificity, and accuracy of QF-PCR in diagnosing fetal chromosomal aneuploidy were 85.7%, 99.81%, and 99.54%, respectively, and the Kappa value for its consistency with the gold standard was 0.88. The sensitivity, specificity, and accuracy of the ultrasound, combined with QF-PCR in diagnosing fetal chromosomal aneuploidy were 95.23%,99.71%, and 99.63%, respectively, and the Kappa value of combined tests’ consistency with the gold standard was 0.91. Conclusion • QF-PCR combined with ultrasound in early pregnancy can effectively improve the accuracy of prenatal diagnosis of fetal chromosome aneuploidy, especially for high-risk pregnant women with a high, positive, predictive value, providing a feasible detection method for clinical practice. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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