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Cumin, a popular spice, is widely used to treat stomach ailments in Central Asia and Xinjiang, China. Cumin essential oil has been found to effectively treat gastric ulcers, but its pharmacodynamic basis remains unclear. In this study, cumin essential oil was directly separated using column chromatography, and its components were identified through multi‐dimensional gas chromatography‐mass spectrometry. Finally, the cumin essential oil was fractionated into E1, E2, and E3. The effects of these fractions on gastric ulcers were studied using an anhydrous ethanol‐induced rat model. The results indicated that the three fractions decreased ulcer index, gastric fluid pH, and pepsin activity to different extents. They lowered the levels of prostaglandin E2, gastrin, and epidermal growth factor in rat serum. According to an analysis of the above indices, E3 fraction had the best anti‐ulcer effect. The detection results of the oxidative stress and inflammatory factors showed that all three fractions relieved the ethanol‐induced oxidative stress and reduced the release of inflammatory factors to varying degrees. The E3 fraction played the most significant role. The E3 fraction was selected to explore the relevant mechanism, and the results showed that E3 fraction significantly prevented the cleaved caspase‐3 and Bax protein levels that were ethanol‐induced and resisted apoptosis induced by ethanol injury. The western blot results for detecting the NF‐κB‐related pathway protein showed that E3 fraction significantly inhibited the activation of p‐p65, p‐IKKβ, and p‐IκBα. The study found that the E3 fraction of cumin essential oil had the most effective anti‐ulcer effect by inhibiting NF‐κB activation and apoptosis, thus reducing inflammation. [ABSTRACT FROM AUTHOR]

In this paper, a high-frequency lamb wave resonator (LWR) with near zero temperature coefficient of frequency (TCF) is designed, fabricated, and measured. The reported resonator is of a bi-layer structure consisting of lithium niobate (LiNbO3 or LN) and silicon dioxide (SiO2), and lithographically patterned aluminum (Al) inter-digitated electrode fingers on top of the bi-layer structure. By adjusting the thickness ratio of LN and SiO2 layers, both the electromechanical coupling ( k 2 ) and the TCF, including both TCF at resonant frequency (TCFr) and TCF at anti-resonant frequency (TCFa), of the thickness shear (TS) type LWR are optimized. Experimental results, which are in excellent agreement with theoretical analysis, show that the fabricated 11.6-GHz LWR achieves a k 2 of 12.2%, with TCFr and TCFa being −4.2 and −5.4 ppm/K over a temperature range from 30 °C to 85 °C, respectively, demonstrating huge potential in applications for future wireless communication systems above 10 GHz. [ABSTRACT FROM AUTHOR]

Our hospital admitted a patient who had difficulty in coagulation even after blood replacement, and the patient had abused caffeine sodium benzoate (CSB) for more than 20 years. Hence, we aimed to explore whether CSB may cause dysfunction in vascular endothelial cells and its possible mechanism. Low, medium, and high concentrations of serum of long‐term CSB intake patients were used to treat HUVECs, with LPS as the positive control. MTT and CCK8 were performed to verify CSB's damaging effect on HUVECs. The expression of ET‐1, ICAM‐1, VCAM‐1, and E‐selectin were measured by ELISA. TUNEL assay and Matrigel tube formation assay were carried out to detect apoptosis and angiogenesis of HUVECs. Flow cytometry was applied to analyze cell cycles and expression of CD11b, PDGF, and ICAM‐1. Expression of PDGF‐BB and PCNA were examined by western blot. The activation of MAPK signaling pathway was detected by qRT‐PCR and western blot. Intracellular Ca2+ density was detected by fluorescent probes. CCK8 assay showed high concentration of CSB inhibited cell viability. Cell proliferation and angiogenesis were inhibited by CSB. ET‐1, ICAM‐1, VCAM‐1, and E‐selectin upregulated in CSB groups. CSB enhanced apoptosis of HUVECs. CD11b, ICAM‐1 increased and PDGF reduced in CSB groups. The expression level and phosphorylation level of MEK, ERK, JUN, and p38 in MAPK pathway elevated in CSB groups. The expression of PCNA and PDGF‐BB was suppressed by CSB. Intracellular Ca2+ intensity was increased by CSB. Abuse of CSB injured HUVECs and caused coagulation disorders. [ABSTRACT FROM AUTHOR]

The protective effect and mechanism of cuminaldehyde, the active ingredient of cumin, was investigated in the indomethacin-induced gastric ulcer rat model. To this end, a control group (CK), indomethacin model group (Model), omeprazole positive control group (Positive), as well as cuminaldehyde low (CUM2), medium (CUM4), and high (CUM6) dose groups were employed, with six mice per group. Indomethacin was continuously administered over 7 days in order to establish the gastric ulcer rat model. The morphology, gastric ulcer index, pepsin activity, and antioxidant properties of gastric tissue were evaluated. Higher cuminaldehyde doses decreased the number of stripe-like ulcers and bleeding spots in gastric tissue. Further, edema and erosion were alleviated, the arrangement of glands tended to be orderly, and inflammatory cell infiltration was suppressed. The ulcer index and pepsin activity gradually decreased in a dose-dependent manner (P<0.01), whereas the ulcer inhibition rate increased. The highest ulcer inhibition rate was observed in the CUM6 group (58.48%). SOD and CAT activity as well as the GSH and NO content in gastric tissue increased to varying degrees, whereas MDA levels decreased under cuminaldehyde treatment (P<0.05 or P<0.01). Serum TNF-α and IL-1β levels, as well as MPO activity decreased, whereas IL-10 and PGE2 levels increased. In conclusion, cuminaldehyde could significantly reduce the size of ulcers and suppress pepsin activity while improving antioxidant and anti-inflammatory activities, thereby protecting gastric tissue against indomethacin. These findings highlight the potential of cuminaldehyde as a treatment for gastric ulcers. [ABSTRACT FROM AUTHOR]

Cumin (Cuminum cyminum L). belongs to the family Apiaceae and the order Apiales, which is a widely grown spice and medicinal plant in Xinjiang province, China. In the current study, whole genome sequencing of C. cyminum was performed using the Illumina HiSeq 4000 platform, and the complete mitogenome sequence was assembled and annotated. We found that the single circular mitogenome of C. cyminum was 246,721 bp in length, and has about 45.5% GC content. It comprised 73 genes in the coding region (35 protein-coding genes, 18 tRNA genes, 3 rRNA genes, and 15 open-reading frames) and a non-coding region. Phylogenetic analysis indicated that C. cyminum is closely related to Daucus carota and the subtribes Daucinae. The mitogenome of C. cyminum revealed its phylogenetic relationships with other species in the Apiaceae family, which would further help in understanding its evolution. [ABSTRACT FROM AUTHOR]

The present study aimed to systematically assess the potential biomarkers in the serum samples of patients with long‐term inhalation of caffeine‐sodium benzoate (CSB). LC–MS was applied to analyze the metabolic profiles of serum samples of patients with the long‐term intake of CSB (n = 35) and other volunteers with no intake of CSB treated as the control group (n = 35). The raw data of metabolic profiles were analyzed via principal component analysis, partial least squares analysis, and orthogonal partial least squares analysis. MBRole 2.0 online tools were used to analyze the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of different metabolites. The serum metabolic profiles showed several metabolites with large variations, including 2‐propyl‐2,4‐pentadienoic acid, 24‐hydroxycholesterol, 3‐O‐sulfogalactosylceramide (d18:1/24:1(15Z)), 3‐O‐sulfogalactosylceramide (d18:1/12:0), 3‐O‐sulfogalactosylceramide (d18:1/14:0), 3a,7a‐dihydroxy‐5b‐cholestan‐26‐al, 3a,7a‐dihydroxy‐5b‐cholestane, 7a,25‐dihydroxycholesterol, bilirubin, and dehydroepiandrosterone sulfate. The Kyoto Encyclopedia of Genes and Genomes pathways involved in metabolism included 'choline metabolism in cancer' and 'glycerophospholipid metabolism'. In conclusion, the present study provides a basis with which to explore the molecular‐specific mechanisms concerning the effects of the long‐term inhalation of CSB on human physical and mental health. [ABSTRACT FROM AUTHOR]

Although many scholars who study the role of racial animus in Americans' political attitudes and policy preferences do so to help us understand national-level politics, (racialized) policy is largely shaped at the state level. States are laboratories of policy innovation whose experiments can exacerbate or ameliorate racial inequality. In this article, we develop state-level scores of racial resentment. By using linear multilevel regression and poststratification weighting techniques and by linking nationally representative survey data with US Census data, we create time-varying, dynamic state-level estimates of racial resentment from 1988 to 2016. These measures enable us to explore the extent to which subnational levels of racial attitudes fluctuate over time and to provide a comparative analysis of state-level racial resentment scores across space and time. We find that states' levels of racial animus change slowly, with some exhibiting increases over time while others do just the opposite. Southern states' reputation for having the highest levels of racial resentment has been challenged by states across various regions of the United States. Many states had their lowest levels of symbolic racism decades ago, contrary to the traditional American narrative of racial progress. [ABSTRACT FROM AUTHOR]

Summary: Nattokinase (NK) is an important serine‐protease with direct fibrinolytic activity involving the prevention of cardiovascular disease as an antithrombotic agent. Dozens of studies have focused on the characterization of intrinsic novel promoters and signal peptides to the secretory production of recombinant proteins in Bacillus subtilis. However, intrinsic genetic elements have several drawbacks, which cannot mediate the production of NK to the desired level. In this study, the genetic elements, which were used to overproduce the recombinant secretory NK, were rationally modified in B. subtilis in a stepwise manner. The first step was to select a suitable signal peptide for the highly efficient secretion of NK. By comparison of the secretory levels mediated by two different signal peptides, which were encoded by the genes of a minor extracellular protease epr (SPepr) and cell‐wall associated protease wapA (SPwapA), respectively, SPwapA was verified as the superior secretory element. Second, P04, which was a synthetic promoter screened from an array of mutants based on the promoter cloned from the operon of a quorum‐sensing associated gene srfA (PsrfA), was paired to SPwapA. The secretory level of NK was obviously augmented by the combination of these two genetic elements. Third, the cis‐acting element CodY‐binding sequence positioned at the 5′UTR was deleted (yielding P08), and thus the secretory level was significantly elevated. The activity of NK, which was defined as fibrinolytic units (FU), reached to a level of 270 FU ml−1. Finally, the superior genetic element composed of P08 and SPwapA was utilized to overproduce NK in the host B. subtilis WB800, which was able to produce the secretory NK at 292 FU ml−1. The strategy established in this study can not only be used to overproduce NK in B. subtilis but also might be a promising pipeline to modify the genetic element for the synthetic secretory system. [ABSTRACT FROM AUTHOR]

In this paper, a high-frequency lamb wave resonator (LWR) with near zero temperature coefficient of frequency (TCF) is designed, fabricated, and measured. The reported resonator is of a bi-layer structure consisting of lithium niobate (LiNbO3 or LN) and silicon dioxide (SiO2), and lithographically patterned aluminum (Al) inter-digitated electrode fingers on top of the bi-layer structure. By adjusting the thickness ratio of LN and SiO2 layers, both the electromechanical coupling ( k 2 ) and the TCF, including both TCF at resonant frequency (TCFr) and TCF at anti-resonant frequency (TCFa), of the thickness shear (TS) type LWR are optimized. Experimental results, which are in excellent agreement with theoretical analysis, show that the fabricated 11.6-GHz LWR achieves a k 2 of 12.2%, with TCFr and TCFa being −4.2 and −5.4 ppm/K over a temperature range from 30 °C to 85 °C, respectively, demonstrating huge potential in applications for future wireless communication systems above 10 GHz. [ABSTRACT FROM AUTHOR]

Cumin ( Cuminum cyminum L). belongs to the family Apiaceae and the order Apiales, which is a widely grown spice and medicinal plant in Xinjiang province, China. In the current study, whole genome sequencing of C. cyminum was performed using the Illumina HiSeq 4000 platform, and the complete mitogenome sequence was assembled and annotated. We found that the single circular mitogenome of C. cyminum was 246,721 bp in length, and has about 45.5% GC content. It comprised 73 genes in the coding region (35 protein-coding genes, 18 tRNA genes, 3 rRNA genes, and 15 open-reading frames) and a non-coding region. Phylogenetic analysis indicated that C. cyminum is closely related to Daucus carota and the subtribes Daucinae . The mitogenome of C. cyminum revealed its phylogenetic relationships with other species in the Apiaceae family, which would further help in understanding its evolution., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Filamentous microalga Tribonema sp. has the advantages of highly resistance to zooplankton-predation, easy harvesting, and high cellular lipid content, in particular large amounts of palmitoleic acid (PA) and eicosapentaenoic acid (EPA). Therefore, Tribonema sp. is considered as a promising biomass feedstock to produce biodiesel and high-value products. In this work, we studied the effect of different concentrations of nitrogen (NaNO₃: 255-3 060 mg/L), phosphorus (K₂HPO₄: 4-240 mg/L), iron ((NH₄)₃FeC₁₂H₁₀O₁₄: 0.6-12 mg/L) and magnesium (MgSO₄: 7.5-450 mg/L) on the biomass, lipid content, and fatty acid composition of Tribonema sp. FACHB-1786, aiming at enhancing cell lipid productivity. The growth of Tribonema sp. had a positive correlation with the concentration of magnesium, and the maximum biomass of Tribonema sp. (under the condition of 450 mg/L MgSO₄) was 8.09 g/L, much greater than those reported in previous studies using the same and other Tribonema species under autotrophic conditions. Different nitrogen concentrations exerted no significant effect on algal growth (P > 0.05), but a higher nitrogen concentration resulted in a greater amount of lipid in the cells. The maximum volumetric productivities of total lipids (319. 6 mg/(L·d)), palmitoleic acid (135.7 mg/(L·d)), and eicosapentaenoic acid (24.2 mg/(L·d)) of Tribonema sp. were obtained when the concentrations of NaNO₃, K₂HPO₄, (NH₄)₃FeC₁₂H₁₀O₁₄, and MgSO₄ were 765 mg/L, 80 mg/L, 6 mg/L, and 75 mg/L, respectively. This study will provide a reference for substrate optimization for Tribonema sp. growth and lipid production.