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47 results on '"Sun, Zhengwen"'

1. High-quality genome of a modern soybean cultivar and resequencing of 547 accessions provide insights into the role of structural variation

Author

Zhang, Caiying, Shao, Zhenqi, Kong, Youbin, Du, Hui, Li, Wenlong, Yang, Zhanwu, Li, Xiangkong, Ke, Huifeng, Sun, Zhengwen, Shao, Jiabiao, Chen, Shiliang, Zhang, Hua, Chu, Jiahao, Xing, Xinzhu, Tian, Rui, Qin, Ning, Li, Junru, Huang, Meihong, Sun, Yaqian, Huo, Xiaobo, Meng, Chengsheng, Wang, Guoning, Liu, Yuan, Ma, Zhiying, Tian, Shilin, and Li, Xihuan

Published
2024
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7. Molecular markers and candidate genes of plant height traits in upland cotton identified by single‐locus and multi‐locus genome‐wide association study

Author

Zhang, Zhen, primary, Wang, Xingyi, additional, Guan, Jiaxin, additional, Zhang, Dongmei, additional, Li, Zhao, additional, Zhang, Meng, additional, Ke, Huifeng, additional, Gu, Qishen, additional, Yang, Jun, additional, Zhang, Yan, additional, Wu, Liqiang, additional, Ma, Zhiying, additional, Wang, Xingfen, additional, and Sun, Zhengwen, additional

Published
2024
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8. Host-induced gene silencing of the Verticillium dahliaethiamine transporter protein gene (VdThit) confers resistance to Verticilliumwilt in cotton

Author

Wang, Qi, Pan, Guoqiang, Wang, Xingfen, Sun, Zhengwen, Guo, Huiming, Su, Xiaofeng, and Cheng, Hongmei

Abstract

Verticilliumwilt (VW), induced by the soil-borne fungus Verticillium dahliae(Vd), poses a substantial threat to a diverse array of plant species. Employing molecular breeding technology for the development of cotton varieties with heightened resistance to VW stands out as one of the most efficacious protective measures. In this study, we successfully generated two stable transgenic lines of cotton (Gossypium hirsutumL.), VdThit-RNAi-1 and VdThit-RNAi-2, using host-induced gene silencing (HIGS) technology to introduce double-stranded RNA (dsRNA) targeting the thiamine transporter protein gene (VdThit). Southern blot analysis confirmed the presence of a single-copy insertion in each line. Microscopic examination showed marked reductions in the colonization and spread of Vd-mCherryin the roots of VdThit-RNAi cotton compared to wild type (WT). The corresponding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant reductions. Real-time quantitative PCR (qRT-PCR) analysis demonstrated a substantial inhibition of VdThitexpression following prolonged inoculation of VdThit-RNAi cotton. Small RNA sequencing (sRNA-Seq) analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic lines. Additionally, the silencing of VdThitby the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in Vd. Under field conditions, VdThit-RNAi transgenic cotton exhibited significantly enhanced disease resistance and yield compared with WT. In summary, our findings underscore the efficacy of HIGS targeting VdThitin restraining the infection and spread of Vdin cotton, thereby potentially enabling the development of cotton breeding as a promising strategy for managing VW.

Published
2024
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9. Expression analysis of the R2R3-MYB gene family in upland cotton and functional study of GhMYB3D5in regulating Verticilliumwilt resistance

Author

Liu, Jie, Wang, Zhicheng, Chen, Bin, Wang, Guoning, Ke, Huifeng, Zhang, Jin, Jiao, Mengjia, Wang, Yan, Xie, Meixia, Li, Yanbin, Zhang, Dongmei, Wang, Xingyi, Gu, Qishen, Sun, Zhengwen, Wu, Liqiang, Wang, Xingfen, Ma, Zhiying, and Zhang, Yan

Abstract

Improving plant resistance to Verticilliumwilt (VW), which causes massive losses in Gossypium hirsutum, is a global challenge. Crop plants need to efficiently allocate their limited energy resources to maintain a balance between growth and defense. However, few transcriptional regulators specifically respond to Verticillium dahliaeand the underlying mechanism has not been identified in cotton. In this study, we found that the that expression of most R2R3-MYB members in cotton is significantly changed by V. dahliaeinfection relative to the other MYB types. One novel R2R3-MYB transcription factor (TF) that specifically responds to V. dahliae, GhMYB3D5, was identified. GhMYB3D5was not expressed in 15 cotton tissues under normal conditions, but it was dramatically induced by V. dahliaestress. We functionally characterized its positive role and underlying mechanism in VW resistance. Upon V. dahliaeinfection, the up-regulated GhMYB3D5 bound to the GhADH1promoter and activated GhADH1expression. In addition, GhMYB3D5 physically interacted with GhADH1 and further enhanced the transcriptional activation of GhADH1. Consequently, the transcriptional regulatory module GhMYB3D5-GhADH1 then promoted lignin accumulation by improving the transcriptional levels of genes related to lignin biosynthesis (GhPAL, GhC4H, Gh4CL, and GhPOD/GhLAC) in cotton, thereby enhancing cotton VW resistance. Our results demonstrated that the GhMYB3D5promotes defense-induced lignin accumulation, which can be regarded as an effective way to orchestrate plant immunity and growth.

Published
2024
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10. High-quality genome assembly and resequencing of modern cotton cultivars provide resources for crop improvement

Author

Ma, Zhiying, Zhang, Yan, Wu, Liqiang, Zhang, Guiyin, Sun, Zhengwen, Li, Zhikun, Jiang, Yafei, Ke, Huifeng, Chen, Bin, Liu, Zhengwen, Gu, Qishen, Wang, Zhicheng, Wang, Guoning, Yang, Jun, Wu, Jinhua, Yan, Yuanyuan, Meng, Chengsheng, Li, Lihua, Li, Xiuxin, Mo, Shaojing, Wu, Nan, Ma, Limei, Chen, Liting, Zhang, Man, Si, Aijun, Yang, Zhanwu, Wang, Nan, Wu, Lizhu, Zhang, Dongmei, Cui, Yanru, Cui, Jing, Lv, Xing, Li, Yang, Shi, Rongkang, Duan, Yihong, Tian, Shilin, and Wang, Xingfen

Published
2021
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12. Revisiting the 2017 Jiuzhaigou (Sichuan, China) Earthquake: Implications for Slip Inversions Based on InSAR Data.

Author

Sun, Zhengwen and Zhao, Yingwen

Subjects
*EARTHQUAKES, *REMOTE-sensing images, *SURFACE potential, *OPTICAL images
Abstract

The 2017 Jiuzhaigou earthquake (Ms = 7.0) struck the eastern Tibetan Plateau and caused extensive concern. However, the reported slip models of this earthquake have distinct discrepancies and cannot provide a good fit for GPS data. The Jiuzhaigou earthquake also presents a good opportunity to investigate the question of how to avoid overfitting of InSAR observations for co-seismic slip inversions. To comprehend this shock, we first used pre-seismic satellite optical images to extract a surface trace of the seismogenic fault, which constitutes the northern segment of the Huya Fault. Then, we collected GPS observations as well as to measure the co-seismic displacements. Lastly, joint inversions were carried out to obtain the slip distribution. Our results showed that the released moment was 5.3 × 1018 N m, equivalent to Mw 6.4 with a rigidity of 30 GPa. The maximum slip at a depth of ~6.8 km reached up to 1.12 m, dominated by left-lateral strike-slip. The largest potential surface rupture occurred in the center of the seismogenic fault with strike- and dip-slip components of 0.4 m and 0.2 m, respectively. Comparison with the focal mechanisms of the 1973 Ms 6.5 earthquake and the 1976 triplet of earthquakes (Mw > 6) on the middle and south segments of the Huya Fault indicated different regional motion and slip mechanisms on the three segments. The distribution of co-seismic landslides had a strong correlation with surface displacements rather than surface rupture. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Genome-Wide Identification of the Alfin-like Gene Family in Cotton (Gossypium hirsutum) and the GhAL19 Gene Negatively Regulated Drought and Salt Tolerance.

Author

Liu, Jie, Wang, Zhicheng, Chen, Bin, Wang, Guoning, Ke, Huifeng, Zhang, Jin, Jiao, Mengjia, Wang, Yan, Xie, Meixia, Gu, Qishen, Sun, Zhengwen, Wu, Liqiang, Wang, Xingfen, Ma, Zhiying, and Zhang, Yan

Subjects
GENE expression, GENE families, ABIOTIC stress, COTTON, OXIDANT status
Abstract

Alfin-like (AL) is a small plant-specific gene family characterized by a PHD-finger-like structural domain at the C-terminus and a DUF3594 structural domain at the N-terminus, and these genes play prominent roles in plant development and abiotic stress response. In this study, we conducted genome-wide identification and analyzed the AL protein family in Gossypium hirsutum cv. NDM8 to assess their response to various abiotic stresses for the first time. A total of 26 AL genes were identified in NDM8 and classified into four groups based on a phylogenetic tree. Moreover, cis-acting element analysis revealed that multiple phytohormone response and abiotic stress response elements were highly prevalent in AL gene promoters. Further, we discovered that the GhAL19 gene could negatively regulate drought and salt stresses via physiological and biochemical changes, gene expression, and the VIGS assay. The study found there was a significant increase in POD and SOD activity, as well as a significant change in MDA in VIGS-NaCl and VIGS-PEG plants. Transcriptome analysis demonstrated that the expression levels of the ABA biosynthesis gene (GhNCED1), signaling genes (GhABI1, GhABI2, and GhABI5), responsive genes (GhCOR47, GhRD22, and GhERFs), and the stress-related marker gene GhLEA14 were regulated in VIGS lines under drought and NaCl treatment. In summary, GhAL19 as an AL TF may negatively regulate tolerance to drought and salt by regulating the antioxidant capacity and ABA-mediated pathway. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Identification and Expression Analysis of EPSPS and BAR Families in Cotton

Author

Li, Zhao, primary, Zhang, Zhen, additional, Liu, Yinbo, additional, Ma, Yuanqi, additional, Lv, Xing, additional, Zhang, Dongmei, additional, Gu, Qishen, additional, Ke, Huifeng, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Ma, Zhiying, additional, Wang, Xingfen, additional, and Sun, Zhengwen, additional

Published
2023
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19. Lysine 2‐Hydroxyisobutyrylation‐ and Succinylation‐Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity

Author

Chen, Bin, primary, Wang, Zhicheng, additional, Jiao, Mengjia, additional, Zhang, Jin, additional, Liu, Jie, additional, Zhang, Dongmei, additional, Li, Yanbin, additional, Wang, Guoning, additional, Ke, Huifeng, additional, Cui, Qiuxia, additional, Yang, Jun, additional, Sun, Zhengwen, additional, Gu, Qishen, additional, Wang, Xingyi, additional, Wu, Jinhua, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Wang, Xingfen, additional, Ma, Zhiying, additional, and Zhang, Yan, additional

Published
2023
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22. Resequencing a core collection of upland cotton identifies genomic variation and loci influencing fiber quality and yield

Author

Ma, Zhiying, He, Shoupu, Wang, Xingfen, Sun, Junling, Zhang, Yan, Zhang, Guiyin, Wu, Liqiang, Li, Zhikun, Liu, Zhihao, Sun, Gaofei, Yan, Yuanyuan, Jia, Yinhua, Yang, Jun, Pan, Zhaoe, Gu, Qishen, Li, Xueyuan, Sun, Zhengwen, Dai, Panhong, Liu, Zhengwen, Gong, Wenfang, Wu, Jinhua, Wang, Mi, Liu, Hengwei, Feng, Keyun, Ke, Huifeng, Wang, Junduo, Lan, Hongyu, Wang, Guoning, Peng, Jun, Wang, Nan, Wang, Liru, Pang, Baoyin, Peng, Zhen, Li, Ruiqiang, Tian, Shilin, and Du, Xiongming

Published
2018
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23. Transcriptome, Ectopic Expression and Genetic Population Analysis Identify Candidate Genes for Fiber Quality Improvement in Cotton

Author

Liu, Zhengwen, primary, Sun, Zhengwen, additional, Ke, Huifeng, additional, Chen, Bin, additional, Gu, Qishen, additional, Zhang, Man, additional, Wu, Nan, additional, Chen, Liting, additional, Li, Yanbin, additional, Meng, Chengsheng, additional, Wang, Guoning, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Ma, Zhiying, additional, Zhang, Yan, additional, and Wang, Xingfen, additional

Published
2023
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24. SEP-like genes of Gossypium hirsutum promote flowering via targeting different loci in a concentration-dependent manner

Author

Chen, Liting, Yan, Yuanyuan, Ke, Huifeng, Zhang, Zihao, Meng, Chengsheng, Ma, Limei, Sun, Zhengwen, Chen, Bin, Liu, Zhengwen, Wang, Guoning, Yang, Jun, Wu, Jinhua, Li, Zhikun, Wu, Liqiang, Zhang, Guiyin, Zhang, Yan, Wang, Xingfen, and Ma, Zhiying

Subjects
Plant Science
Abstract

SEP genes are famous for their function in the morphological novelty of bisexual flowers. Although the diverse functions of SEP genes were reported, only the regulatory mechanisms underlying floral organ development have been addressed. In this study, we identified SEP-like genes in Gossypium and found that SEP3 genes were duplicated in diploid cotton varieties. GhSEP4.1 and GhSEP4.2 were abundantly transcribed in the shoot apical meristem (SAM), but only GhSEP4.2 was expressed in the leaf vasculature. The expression pattern of GhSEPs in floral organs was conserved with that of homologs in Arabidopsis, except for GhSEP2 that was preponderantly expressed in ovules and fibers. The overexpression and silencing of each single GhSEP gene suggested their distinct role in promoting flowering via direct binding to GhAP1 and GhLFY genomic regions. The curly leaf and floral defects in overexpression lines with a higher expression of GhSEP genes revealed the concentration-dependent target gene regulation of GhSEP proteins. Moreover, GhSEP proteins were able to dimerize and interact with flowering time regulators. Together, our results suggest the dominant role of GhSEP4.2 in leaves to promote flowering via GhAP1-A04, and differently accumulated GhSEP proteins in the SAM alternately participate in forming the dynamic tetramer complexes to target at the different loci of GhAP1 and GhLFY to maintain reproductive growth. The regulatory roles of cotton SEP genes reveal their conserved and diversified functions.

Published
2022
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26. Pin1/ YAP pathway mediates matrix stiffness‐induced epithelial–mesenchymal transition driving cervical cancer metastasis via a non‐Hippo mechanism

Author

Yang, Long, primary, Li, Jingwen, additional, Zang, Guangchao, additional, Song, Sijie, additional, Sun, Zhengwen, additional, Li, Xinyue, additional, Li, Yuanzhu, additional, Xie, Zhenhong, additional, Zhang, Guangyuan, additional, Gui, Ni, additional, Zhu, Shu, additional, Chen, Tingting, additional, Cai, Yikui, additional, and Zhao, Yinping, additional

Published
2022
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31. GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Author

Zhang, Man, primary, Wang, Xingfen, additional, Yang, Jun, additional, Wang, Zhicheng, additional, Chen, Bin, additional, Zhang, Xinyu, additional, Zhang, Dongmei, additional, Sun, Zhengwen, additional, Wu, Jinhua, additional, Ke, Huifeng, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Zhang, Yan, additional, and Ma, Zhiying, additional

Published
2022
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33. A large‐scale genomic association analysis identifies a fragment in Dt11 chromosome conferring cotton Verticillium wilt resistance

Author

Zhang, Yan, primary, Chen, Bin, additional, Sun, Zhengwen, additional, Liu, Zhengwen, additional, Cui, Yanru, additional, Ke, Huifeng, additional, Wang, Zhicheng, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Wang, Guoning, additional, Li, Zhikun, additional, Yang, Jun, additional, Wu, Jinhua, additional, Shi, Rongkang, additional, Liu, Song, additional, Wang, Xingfen, additional, and Ma, Zhiying, additional

Published
2021
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34. Tissue‐specific expression of GhnsLTPs identified via GWAS sophisticatedly coordinates disease and insect resistance by regulating metabolic flux redirection in cotton

Author

Chen, Bin, primary, Zhang, Yan, additional, Sun, Zhengwen, additional, Liu, Zhengwen, additional, Zhang, Dongmei, additional, Yang, Jun, additional, Wang, Guoning, additional, Wu, Jinhua, additional, Ke, Huifeng, additional, Meng, Chengsheng, additional, Wu, Lizhu, additional, Yan, Yuanyuan, additional, Cui, Yanru, additional, Li, Zhikun, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Wang, Xingfen, additional, and Ma, Zhiying, additional

Published
2021
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35. Cotton GhSSI2 isoforms from the stearoyl acyl carrier protein fatty acid desaturase family regulate Verticillium wilt resistance

Author

Mo, Shaojing, primary, Zhang, Yan, additional, Wang, Xingfen, additional, Yang, Jun, additional, Sun, Zhengwen, additional, Zhang, Dongmei, additional, Chen, Bin, additional, Wang, Guoning, additional, Ke, Huifeng, additional, Liu, Zhengwen, additional, Meng, Chengsheng, additional, Li, Zhikun, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Duan, Huijun, additional, and Ma, Zhiying, additional

Published
2021
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36. A Novel Stable QTL in Chromosome A04 for Salt Tolerance at the Seed Germination Stage of Upland Cotton via a Resequencing-Based High-Density Genetic Map

Author

Gu, Qishen, primary, Ke, Huifeng, additional, Liu, Chenchen, additional, Lv, Xing, additional, Sun, Zhengwen, additional, Liu, Zhengwen, additional, Rong, Wei, additional, Yang, Jun, additional, Zhang, Yan, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Wang, Xingfen, additional, and Ma, Zhiying, additional

Published
2021
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37. Evolution, Expression and Functional Analysis of Cultivated Allotetraploid Cotton DIR Genes

Author

Liu, Zhengwen, primary, Wang, Xingfen, additional, Sun, Zhengwen, additional, Zhang, Yan, additional, Meng, Chengsheng, additional, Chen, Bin, additional, Wang, Guoning, additional, Ke, Huifeng, additional, Wu, Jinhua, additional, Yan, Yuanyuan, additional, Wu, Liqiang, additional, Li, Zhikun, additional, Yang, Jun, additional, Zhang, Guiyin, additional, and Ma, Zhiying, additional

Published
2020
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42. Genome-wide association study discovered genetic variation and candidate genes of fibre quality traits in Gossypium hirsutum L.

Author

Sun, Zhengwen, primary, Wang, Xingfen, additional, Liu, Zhengwen, additional, Gu, Qishen, additional, Zhang, Yan, additional, Li, Zhikun, additional, Ke, Huifeng, additional, Yang, Jun, additional, Wu, Jinhua, additional, Wu, Liqiang, additional, Zhang, Guiyin, additional, Zhang, Caiying, additional, and Ma, Zhiying, additional

Published
2017
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43. Identification of SNPs and Candidate Genes Associated With Salt Tolerance at the Seedling Stage in Cotton (<italic>Gossypium hirsutum</italic> L.).

Author

Sun, Zhengwen, Li, Hanli, Zhang, Yan, Li, Zhikun, Ke, Huifeng, Wu, Liqiang, Zhang, Guiyin, Wang, Xingfen, and Ma, Zhiying

Subjects
SINGLE nucleotide polymorphisms, COTTON, PLANT chromosomes
Abstract

Salt tolerance in cotton is highly imperative for improvement in the response to decreasing farmland and soil salinization. However, little is known about the genetic basis underlying salt tolerance in cotton, especially the seedling stage. In this study, we evaluated two salt-tolerance-related traits of a natural population comprising 713 upland cotton (Gossypium hirsutum L.) accessions worldwide at the seedling stage and performed a genome-wide association study (GWAS) to identify marker-trait associations under salt stress using the Illumina Infinium CottonSNP63K array. A total of 23 single nucleotide polymorphisms (SNPs) that represented seven genomic regions on chromosomes A01, A10, D02, D08, D09, D10, and D11 were significantly associated with the two salt-tolerance-related traits, relative survival rate (RSR) and salt tolerance level (STL). Of these, the two SNPs i46598Gh and i47388Gh on D09 were simultaneously associated with the two traits. Based on all loci, we screened 280 possible candidate genes showing different expression levels under salt stress. Most of these genes were involved in transcription factors, transporters and enzymes and were previously reported as being involved in plant salt tolerance, such as NAC, MYB, NXH, WD40, CDPK, LEA, and CIPK. We further validated six putative candidate genes by qRT-PCR and found a differential expression level between salt-tolerant and salt-sensitive varieties. Our findings provide valuable information for enhancing the understanding of complicated mechanisms of salt tolerance in G. hirsutum seedlings and cotton salt tolerance breeding by molecular marker-assisted selection. [ABSTRACT FROM AUTHOR]

Published
2018
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47. Pin1/YAP pathway mediates matrix stiffness-induced epithelial-mesenchymal transition driving cervical cancer metastasis via a non-Hippo mechanism.

Author

Yang L, Li J, Zang G, Song S, Sun Z, Li X, Li Y, Xie Z, Zhang G, Gui N, Zhu S, Chen T, Cai Y, and Zhao Y

Abstract

Cervical cancer metastasis is an important cause of death in cervical cancer. Previous studies have shown that epithelial-mesenchymal transition (EMT) of tumors promotes its invasive and metastatic capacity. Alterations in the extracellular matrix (ECM) and mechanical signaling are closely associated with cancer cell metastasis. However, it is unclear how matrix stiffness as an independent cue triggers EMT and promotes cervical cancer metastasis. Using collagen-coated polyacrylamide hydrogel models and animal models, we investigated the effect of matrix stiffness on EMT and metastasis in cervical cancer. Our data showed that high matrix stiffness promotes EMT and migration of cervical cancer hela cell lines in vitro and in vivo. Notably, we found that matrix stiffness regulates yes-associated protein (YAP) activity via PPIase non-mitotic a-interaction 1 (Pin1) with a non-Hippo mechanism. These data indicate that matrix stiffness of the tumor microenvironment positively regulates EMT in cervical cancer through the Pin1/YAP pathway, and this study deepens our understanding of cervical cancer biomechanics and may provide new ideas for the treatment of cervical cancer., (© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.)

Published
2022
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