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5. Design of Co Nanoparticles‐Encapsulated by Boron and Nitrogen Co‐Doped Carbon Nanosheets as Highly Efficient Electrocatalyst for Oxygen Reduction Reaction

Author

Niu, Wen‐Jun, primary, Sun, Qiao‐Qiao, additional, Wang, Ya‐Ping, additional, Gu, Bing‐Ni, additional, Liu, Ming‐Jin, additional, He, Jin‐Zhong, additional, Chen, Jiang‐Lei, additional, Chung, Chia‐Chen, additional, Liu, Wen‐Wu, additional, and Chueh, Yu‐Lun, additional

Published
2021
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8. Cloning, Expression of Thermophilic Fungus Neosartorya fischeri P1 Lipase Gene and Analysis of its Enzymatic Property.

Author

SUN Qiao-qiao, TAN Xiao, LV Yi, HUANG Huo-qing, ZHANG Hui-tu, and LU Fu-ping

Abstract

A thermophilic fungus that have high lipase activity was screened from the acid waste water of a tine mine in Yunnan Province. It was identified as Neosartorya fischeri through morphological observation and internal transcribed spacer (ITS) sequence analysis, and named Neosartorya fischeri PI. The lipase gene Lip024 was cloned from N. fischeri P1 genome and expressed in Escherichia coli BL21 ( DE3) and Pichia pastoris GS115, with the activity of recombinant lipase 0.09U/mL and 0.26U/mL, respectively. High density fermentation was performed in 3.7 L fermenter for the recombinant strain, and the recombinant enzyme activity reached 5.22U/mL. Lip024 was purified to homogeneity by ultrafiltration and anion exchange chromatography. The optimal pH of recombinant Lip024 was pH 7.0, and the enzyme showed high stability at pH3.0 ˜ 7.0. The Lip024 was highly thermostable, and the optimal temperature was found to be 50°C. In addition, Ca2+ and Mg2+can significantly improve the activity of Lip024 [ABSTRACT FROM AUTHOR]

Published
2014
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