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The experimental teaching is an important way in the process of fostering student innovation ability, and the experimental teaching management is the key issue to achieving achieve the goal of the training objectives, therefore, research on the experimental teaching management is growing, and becomes the focus of higher education in recent years. In this article, combining the qualitative analysis with the quantitative analysis and literature retrieval, the author made an intensive study of research status in growth of the papers, keywords, journal, disciplines, and institutions, described the research emphases on change of function, the reform of management, and development and information system, deeply analyzed the existing problems such as lack of theory guidance, confusion of teaching and management, instable faculties and so on. It also put forward some advices such as centralized management, joining the theory support and enhancing cross of disciplines for future research. [ABSTRACT FROM AUTHOR]

Shandong provincial education department and financial department have implemented the construction project of teaching experimental center of key discipline in universities since 2007. The project has played a promoting role in keeping Shandong higher education developing healthy, coordinated and sustainable. This article introduces some basic situation, construction ideas, construction content and achievement of the construction project of teaching experimental center of key discipline in our university, puts forward some advices that construction ideas should adapt to the development planning of discipline, construction measures should adhere to four principles, construction progress should handle three relations, and construction target correspond to performance evaluation system. [ABSTRACT FROM AUTHOR]

Background: Immunological dysfunction-induced low-grade inflammation is regarded as one of the predominant pathogenetic mechanisms in post-infectious irritable bowel syndrome (PI-IBS). γδ T cells play a crucial role in innate and adaptive immunity. Adenosine receptors expressed on the surface of γδ T cells participate in intestinal inflammation and immunity regulation., Aim: To investigate the role of γδ T cell regulated by adenosine 2A receptor (A2AR) in PI-IBS., Methods: The PI-IBS mouse model has been established with Trichinella spiralis (T. spiralis) infection. The intestinal A2AR and A2AR in γδ T cells were detected by immunohistochemistry, and the inflammatory cytokines were measured by western blot. The role of A2AR on the isolated γδ T cells, including proliferation, apoptosis, and cytokine production, were evaluated in vitro . Their A2AR expression was measured by western blot and reverse transcription polymerase chain reaction (RT-PCR). The animals were administered with A2AR agonist, or A2AR antagonist. Besides, γδ T cells were also injected back into the animals, and the parameters described above were examined, as well as the clinical features. Furthermore, the A2AR-associated signaling pathway molecules were assessed by western blot and RT-PCR., Results: PI-IBS mice exhibited elevated ATP content and A2AR expression ( P < 0.05), and suppression of A2AR enhanced PI-IBS clinical characteristics, indicated by the abdominal withdrawal reflex and colon transportation test. PI-IBS was associated with an increase in intestinal T cells, and cytokine levels of interleukin-1 (IL-1), IL-6, IL-17A, and interferon-α (IFN-α). Also, γδ T cells expressed A2AR in vitro and generated IL-1, IL-6, IL-17A, and IFN-α, which can be controlled by A2AR agonist and antagonist. Mechanistic studies demonstrated that the A2AR antagonist improved the function of γδ T cells through the PKA/CREB/NF-κB signaling pathway., Conclusion: Our results revealed that A2AR contributes to the facilitation of PI-IBS by regulating the function of γδ T cells via the PKA/CREB/NF-κB signaling pathway., Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article., (©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.)

Although laboratory fish are increasingly used in genetics and other life science research fields, standard quality control and supervision are needed. In China, laboratory animals are all put into a strict licensing and quality management system by the government. The standardization of genetic quality control is crucial to a laboratory fish quality control management system. The goal of Laboratory Animal Regulation is to control genetic quality, avoid hereditary degeneration and genetic drift, and circumvent experimental errors. To achieve this goal, Laboratory Animal Regulations are being developed by consulting experimental data and research findings throughout the world, combining the best known practices in laboratory fish production, and consulting specialists. A new set of laboratory fish genetic quality standards focusing on zebrafish and swordtail fish has been established as a reference for scientific researchers. The new standards define inbred and outbred zebrafish and swordtail fish hereditary classifications, naming principles, breeding methods, and hereditary quality surveying. The new standards provide a frame of reference for laboratory fish users and managers.