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Byline: Bing-Bing. Ren, Kui. Jiang, Tao. Wang, Da-Qing. Sun Gastric antral web (GAW) is a rare anomaly of the gastric antrum and can result in gastric outlet obstruction. Currently, endoscopic [...]

Abstract: To further elucidate the mechanism and determine the biomarker of neuropathy induced by carbon disulfide (CS2), we performed a longitudinal observational study of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD) and total antioxidative capacity (T-AOC) in rat cerebral cortex, hippocampus, spinal cord and serum after 0, 2, 4, 8 and 12 weeks of CS2 administration. CS2 exposure was found to markedly increase ROS and MDA levels in cerebral cortex, hippocampus, spinal cord and serum of rats in both time and symptom-dependent manners. Although SOD activities slightly increased, there was a decrease in the GSH contents and GSH-Px, CAT activities in cerebral cortex, hippocampus, spinal cord and serum after 2, 4, 8 or 12 weeks’ CS2 intoxication and at gait score of 2, 3, or 4. The activities of T-AOC also decreased in all three nerve tissues and serum as time went on and symptom developed. Furthermore, significant correlations between LPO and gait abnormality were observed as symptom developed. Oxidation stress also resulted in Ca2+ concentrations and calmodulin (CaM) levels increases in cerebral cortex, hippocampus and spinal cord. Thus, CS2 intoxication was associated with elevation of lipid peroxidation (LPO) and reduction of antioxidant status, and the time and symptom-dependent changes of these indexes in rats’ nerve tissues and serum suggested that ROS and concomitant LPO, at least in part, were involved in CS2-induced neuropathy. [Copyright &y& Elsevier]

Established undamped mechanical model and mathematical model of horizontal vibrating centrifuge. The main vibration spring stiffness gets lower due to the temperature rise of bearing, which leads to amplitude-frequency characteristic curve change, shell and screen-basket amplitude instability. Found the fixed point on the screen-basket amplitude-frequency characteristic curve is not affected by the main vibration spring stiffness. Combined with the antiresonant frequency in the case of higher temperature of bearings, in order to stablize the amplitude of screen-basket and the minimum amplitude of shell and the less stress of the main vibration spring, and put forward that the system natural frequency of shell and vibration frequency is equal and slightly lower than natural frequency of the screen-basket, and verify such conclusion by experiments. [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical effectiveness of Sanmiao Powder (SP) combined fibrinogenase for injection in treatment of acute deep venous thrombosis (ADVT) of lower limbs., Methods: Eighty patients with ADVT were randomly assigned to two groups according to the disease course (within 7 days or 7-28 days), 40 in each group. Every time phase was also divided into two groups, i. e., one group treated with fibrinogenase for injection alone (Group A and C) and another group treated with fibrinogenase for injection + SP administration (Group B and D) , 20 in each group. The clinical effectiveness was observed after 2-week treatment., Results: The fibrinogenase for injection + SP administration showed better effects in alleviating the swelling of limbs, relieving pain, and lowering fibrinogen. Better effects were obtained in the group with the disease course less than 7 days., Conclusion: Better effect on ADVT was obtained by integrative medicine.

Aim: To explore the effects of ovalbumin atomization on the T lymphocyte subsets in lung and spleen of BALB/c mice with intestinal microflora disruption resulted from antibiotic therapy., Methods: Thirty two female BALB/c mice were devided at random into 4 groups with eight mice in each. They were microbiota disruption group, microbiota disruption and challenge group, challenge group and control group. On day 14, the levels of Th1, Th2, Th17 and CD4+, CD25+, Foxp3+ regulatory T cells (Tregs) in lung and spleen were detected by flow cytometry., Results: In microbiota disruption and challenge group, Th2 and Th17 cell levels in lung were increased and no difference was found of Th1, Th2 as well as Th17 cell levels in spleen compared with those in the control group. The levels of Tregs were both decreased in lung and spleen of the mice in microbiota disruption group as well as microbiota disruption and challenge group., Conclusion: The allergic airway response driven by Th2 and Th17 cells polarization can be induced by atomization with ovalbumin in mice with intestinal microflora disruption resulted from antibiotic therapy. It's maybe associated with insufficient function of Tregs.

Aim: The mice in which the intestinal microflora disruption resulted from antibiotic therapy are challenged by atomization with ovalbumin (OVA) to investigate the relation of allergic airway response and intestinal microflora disruption., Methods: One hundred and twelve female BALB/c mice were divided at random into 6 groups. They were microbiota disruption I group, control I group, microbiota disruption II group, microbiota disruption and challenge group, challenge group and control II group. Cecal contents were collected for quantitative analysis of the intestinal microflora in mice in the former two groups and in mice in the latter four groups on day 6 and day 14, respectively. On day 14, the bronchoalveolar lavage fluid (BALF) was collected for cells counting. OVA-specific IgE in BALF and sera was detected by ELISA. Parts of lungs were collected for histopathology and detection of Th1 and Th2 cell levels by flow of cytometry., Results: The mice which were given antibiotics suffered from intestinal microbiota disruption. In microbiota disruption and challenge group, eosinophil and lymphocyte infiltration was significant and mucus secretion was increased in lung. The number of total cells, eosinophils, lymphocytes, neutrophils and OVA-specific IgE level were increased in BALF in microbiota disruption and challenge group. Th2 cell levels were increased and Th1 cell levels were not significantly different in microbiota disruption and challenge group compared with those in the control II group., Conclusion: The allergic (Th2) immune response can be induced by atomization with ovalbumin in the mice in which the intestinal microflora disruption is resulted from antibiotic therapy. The result suggests that the intestinal microflora disruption is a risk factor for allergy and asthma.

The cDNA sequence of bovine prochymosin gene was cloned and sequenced from the abomasums of suckling calf by RT-PCR. The sequence was aligned and bioinformatically analyzed with related sequences in GenBank. The result of sequence analysis revealed that the gene was determined to bovine prochymosin B gene and had the high level of homology with prochymosin gene of other known mammals. The base bias of 18 species of prochymosin gene reduced according to codon position, and the gene provided us with excellent material of phylogenetic research. Thus, the phylogenetic tree of 18 species of prochymosin gene was used to discuss and offer testimony to phylogenetic relationship of 11 mammals.

Abstract: The major flavor substances Lactic acid bacteria produced include the buttermilk aroma butanedione, the yoghurt flavor acetaldehyde and the amino acid. Metabolic engineering in LAB had focused primarily on rerouting of pyruvate metabolism towards butanedione or acetaldehyde to improve the flavor of fermented milk, which has considerable economic value. The typical yogurt flavor is caused by acetaldehyde produced through many different pathways. The attention was focused on one specific reaction for acetaldehyde formation catalyzed by serine hydroxymethyltransferase, which encoded by the glyA gene. In addition, the efficient conversion of glucose into acetaldehyde was achieved by over-expression of pyruvate decarboxylase and NADH oxidase in LAB. The concentration of acetaldehyde derived from this regulation is higher than the other strategy. As for the regulation of butanedione, it was focused on combinating inactivation of alpha-acetolactate decarboxylase with low lactate dehydrogenase activity, or with overproduction of NADH-oxidase, or with overproduction of alpha-acetolactate synthase. Then the possibility of co-regulation with certain three kinds of enzyme above was recommended.

Objective: To study the changes of IL-12 produced by dendritic cells in peripheral blood in children with Henoch-Schonlein purpura (HSP), and to explore its influence on TH1/TH2 balance in order to elucidate its significance in the pathogenesis of HSP., Methods: The levels of interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and interleukin-12 (IL-12) in plasma were determined by ELISA in 60 HSP children (HSP group) and 21 healthy children (Control group). Peripheral blood mononuclear cells (PBMC) of 22 HSP patients and 21 healthy children were cultured in vitro and then were transformed into dendritic cells. The levels of IL-12 in the supernatant were detected by ELISA and the positive expression rate of CD1a(+) was detected by indirect immunofluorescence procedure., Results: 1) The levels of IFN-gamma and the ratio of IFN-gamma/IL-4 in plasma of the HSP group were lower than those of the Control group (IFN-gamma 30.59 +/- 11.27 pg/mL vs 43.38 +/- 19.19 pg/mL; IFN-gamma/IL-4 ratio 0.70 +/- 0.28 vs 1.33 +/- 0.57) (P < 0.01). The levels of IL-12 in the HSP group were also lower than those of the Control group (153.95 +/- 91.88 pg/mL vs 323.06 +/- 162.34 pg/mL; P < 0.01). In contrast, the levels of IL-4 were higher than those of the Control group (45.08 +/- 9.19 pg/mL vs 32.95 +/- 7.10 pg/mL; P < 0.01). The plasma levels of IL-12 positively correlated with the IFN-gamma levels (r=0.52, P < 0.01) and the ratio of IFN-gamma/IL-4 (r=0.43, P < 0.01) in the HSP group. 2) The IL-12 levels in the supernatant of the HSP group were lower than those of the Control group (357.06 +/- 153.56 pg/mL vs 489.80 +/- 213.45 pg/mL; P < 0.05), and had a positive correlation with the plasma IL-12 levels (r=0.74, P < 0.01). 3) The positive expression rate of CD1a(+) of the HSP group was lower than that of the Control group [(27.42 +/- 10.75)% vs (35.68 +/- 12.18)%; P < 0.05], and positively correlated with the IL-12 levels in the supernatants (r=0.57, P < 0.01) and in plasma (r=0.68, P < 0.01)., Conclusions: There was an imbalance of TH1/TH2 in HSP children. The decrease of TH1 function had a positive correlation with the low levels of IL-12 in plasma, while the latter correlated closely with decreased number and / or function of dendritic cells, suggesting that the decreased number and / or function of dendritic cells in peripheral blood resulted in the imbalance of TH1/TH2 indirectly.