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The present study was carried out to investigate the methanolic extract of leaf of Erioglossum rubiginosum and its fractionates (petroleum ether, carbontetrachloride and chloroform fractions) for their in vitro antioxidant, thrombolytic and cytotoxic activities. Among the different fractions , the carbontetrachloride soluble fraction (CTF) showed significant antioxidant activity (IC 50 =30.32±0.08 μg/ml) as compared to standard ascorbic acid (IC 50 =5.87±0.21 μg/ml) and BHT (IC 50 =27.50±0.64 μg/ml). The extract also inhibited 58.44±0.97% lipid from peroxidation and reveled excellent phenolic profile of about 128.56±1.02 mg of GAE /g of extract. In the thrombolytic assay the highest % of clot lysis was exhibited by the chloroform soluble fraction (CF) (28.57±0.83%), while for streptokinase it was about 66.77±0.92%. The LC 50 values were found within the range of 12.92 to 80.06 μg/ml , while different solvent fraction s were tested for cytotoxicity against brine shrimp nauplii. This is the first report of antioxidant, thrombolytic and cytotoxic activities of E. rubiginosum . Dhaka Univ. J. Pharm. Sci. 12(2): 153-158, 2013 (December) DOI: http://dx.doi.org/10.3329/dujps.v12i2.17612

Aims The present study aimed at assessing the effect of methanol extract of Erioglossum rubiginosum, a widely used shrub like plant in folkloric medicine, in experimentally induced inflammation, using human red blood cell (HRBC) membrane stabilization as study method. Methods The methanol extract of leaves of E. rubiginosum and its pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for lysis of erythrocytes and % of inhibition in hypotonic solution and heat induced lysis, using acetyl salicylic acid as standard drug, in an in vitro model. Results At the concentration of 1 mg/ml, methanol extract and its pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates significantly inhibited hypotonic solution induced lysis of the human red blood cell membrane with values of 0.428 ± 0.005, 0.204 ± 0.003, 0.233 ± 0.002, 0.411 ± 0.003, 0.439 ± 0.003% respectively; which were comparable to the standard drug acetyl salicylic acid (0.1 mg/ml), 0.166 ± 0.003. In case of heat induced HRBC heamolysis, the plant extracts also showed significant activity where the values of inhibitory actions were 14.46 ± 0.344, 28.23 ± 0.315, 25.18 ± 0.303, 17.09 ± 0.365, 13.44 ± 0.470% for methanol extract, pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates respectively. Conclusion It can be postulated from the observed results that the membrane stabilizing action and inhibition of erythrocyte lysis property of E. rubiginosum may be the possible mechanism of action of its anti-inflammatory activity. So, further studies are suggested to evaluate the anti-inflammatory and analgesic activities of the plant.