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2. The DNA sequence and biological annotation of human chromosome 1

Author

Gregory, S. G., Barlow, K. F., McLay, K. E., Kaul, R., Swarbreck, D., Dunham, A., Scott, C. E., Howe, K. L., Woodfine, K., Spencer, C. C. A., Jones, M. C., Gillson, C., Searle, S., Zhou, Y., Kokocinski, F., McDonald, L., Evans, R., Phillips, K., Atkinson, A., Cooper, R., Jones, C., Hall, R. E., Andrews, T. D., Lloyd, C., Ainscough, R., Almeida, J. P., Ambrose, K. D., Anderson, F., Andrew, R. W., Ashwell, R. I. S., Aubin, K., Babbage, A. K., Bagguley, C. L., Bailey, J., Beasley, H., Bethel, G., Bird, C. P., Bray-Allen, S., Brown, J. Y., Brown, A. J., Buckley, D., Burton, J., Bye, J., Carder, C., Chapman, J. C., Clark, S. Y., Clarke, G., Clee, C., Cobley, V., Collier, R. E., Corby, N., Coville, G. J., Davies, J., Deadman, R., Dunn, M., Earthrowl, M., Ellington, A. G., Errington, H., Frankish, A., Frankland, J., French, L., Garner, P., Garnett, J., Gay, L., Ghori, M. R. J., Gibson, R., Gilby, L. M., Gillett, W., Glithero, R. J., Grafham, D. V., Griffiths, C., Griffiths-Jones, S., Grocock, R., Hammond, S., Harrison, E. S. I., Hart, E., Haugen, E., Heath, P. D., Holmes, S., Holt, K., Howden, P. J., Hunt, A. R., Hunt, S. E., Hunter, G., Isherwood, J., James, R., Johnson, C., Johnson, D., Joy, A., Kay, M., Kershaw, J. K., Kibukawa, M., Kimberley, A. M., King, A., Knights, A. J., Lad, H., Laird, G., Lawlor, S., Leongamornlert, D. A., Lloyd, D. M., Loveland, J., Lovell, J., Lush, M. J., Lyne, R., Martin, S., Mashreghi-Mohammadi, M., Matthews, L., Matthews, N. S. W., McLaren, S., Milne, S., Mistry, S., Nickerson, T., O'Dell, C. N., Oliver, K., Palmeiri, A., Palmer, S. A., Parker, A., Patel, D., Pearce, A. V., Peck, A. I., Pelan, S., Phelps, K., Phillimore, B. J., Plumb, R., Rajan, J., Raymond, C., Rouse, G., Saenphimmachak, C., Sehra, H. K., Sheridan, E., Shownkeen, R., Sims, S., Skuce, C. D., Smith, M., Steward, C., Subramanian, S., Sycamore, N., Tracey, A., Tromans, A., Van Helmond, Z., Wall, M., Wallis, J. M., White, S., Whitehead, S. L., Wilkinson, J. E., Willey, D. L., Williams, H., Wilming, L., Wray, P. W., Wu, Z., Coulson, A., Vaudin, M., Sulston, J. E., Durbin, R., Hubbard, T., Wooster, R., Dunham, I., Carter, N. P., McVean, G., Ross, M. T., Harrow, J., Olson, M. V., Beck, S., Rogers, J., and Bentley, D. R.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): S. G. Gregory (corresponding author) [1, 2]; K. F. Barlow [1]; K. E. McLay [1]; R. Kaul [3]; D. Swarbreck [1]; A. Dunham [1]; C. E. Scott [1]; K. [...]

Published
2006
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3. The DNA sequence and analysis of human chromosome 13

Author

Dunham, A., Matthews, L. H., Burton, J., Ashurst, J. L., Howe, K. L., Ashcroft, K. J., Beare, D. M., Burford, D. C., Hunt, S. E., Griffiths-Jones, S., Jones, M. C., Keenan, S. J., Oliver, K., Scott, C. E., Ainscough, R., Almeida, J. P., Ambrose, K. D., Andrews, D. T., Ashwell, R. I. S., Babbage, A. K., Bagguley, C. L., Bailey, J., Bannerjee, R., Barlow, K. F., Bates, K., Beasley, H., Bird, C. P., Bray-Allen, S., Brown, A. J., Brown, J. Y., Burrill, W., Carder, C., Carter, N. P., Chapman, J. C., Clamp, M. E., Clark, S. Y., Clarke, G., Clee, C. M., Clegg, S. C. M., Cobley, V., Collins, J. E., Corby, N., Coville, G. J., Deloukas, P., Dhami, P., Dunham, I., Dunn, M., Earthrowl, M. E., Ellington, A. G., Faulkner, L., Frankish, A. G., Frankland, J., French, L., Garner, P., Garnett, J., Gilbert, J. G. R., Gilson, C. J., Ghori, J., Grafham, D. V., Gribble, S. M., Griffiths, C., Hall, R. E., Hammond, S., Harley, J. L., Hart, E. A., Heath, P. D., Howden, P. J., Huckle, E. J., Hunt, P. J., Hunt, A. R., Johnson, C., Johnson, D., Kay, M., Kimberley, A. M., King, A., Laird, G. K., Langford, C. J., Lawlor, S., Leongamornlert, D. A., Lloyd, D. M., Lloyd, C., Loveland, J. E., Lovell, J., Martin, S., Mashreghi-Mohammadi, M., McLaren, S. J., McMurray, A., Milne, S., Moore, M. J. F., Nickerson, T., Palmer, S. A., Pearce, A. V., Peck, A. I., Pelan, S., Phillimore, B., Porter, K. M., Rice, C. M., Searle, S., Sehra, H. K., Shownkeen, R., Skuce, C. D., Smith, M., Steward, C. A., Sycamore, N., Tester, J., Thomas, D. W., Tracey, A., Tromans, A., Tubby, B., Wall, M., Wallis, J. M., West, A. P., Whitehead, S. L., Willey, D. L., Wilming, L., Wray, P. W., Wright, M. W., Young, L., Coulson, A., Durbin, R., Hubbard, T., Sulston, J. E., Beck, S., Bentley, D. R., Rogers, J., and Ross, M. T.

Published
2004
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4. DNA sequence and analysis of human chromosome 9

Author

Humphray, S. J., Oliver, K., Hunt, A. R., Plumb, R. W., Loveland, J. E., Howe, K. L., Andrews, T. D., Searle, S., Hunt, S. E., Scott, C. E., Jones, M. C., Ainscough, R., Almeida, J. P., Ambrose, K. D., Ashwell, R. I. S., Babbage, A. K., Babbage, S., Bagguley, C. L., Bailey, J., Banerjee, R., Barker, D. J., Barlow, K. F., Bates, K., Beasley, H., Beasley, O., Bird, C. P., Bray-Allen, S., Brown, A. J., Brown, J. Y., Burford, D., Burrill, W., Burton, J., Carder, C., Carter, N. P., Chapman, J. C., Chen, Y., Clarke, G., Clark, S. Y., Clee, C. M., Clegg, S., Collier, R. E., Corby, N., Crosier, M., Cummings, A. T., Davies, J., Dhami, P., Dunn, M., Dutta, I., Dyer, L. W., Earthrowl, M. E., Faulkner, L., Fleming, C. J., Frankish, A., Frankland, J. A., French, L., Fricker, D. G., Garner, P., Garnett, J., Ghori, J., Gilbert, J. G. R., Glison, C., Grafham, D. V., Gribble, S., Griffiths, C., Griffiths-Jones, S., Grocock, R., Guy, J., Hall, R. E., Hammond, S., Harley, J. L., Harrison, E. S. I., Hart, E. A., Heath, P. D., Henderson, C. D., Hopkins, B. L., Howard, P. J., Howden, P. J., Huckle, E., Johnson, C., Johnson, D., Joy, A. A., Kay, M., Keenan, S., Kershaw, J. K., Kimberley, A. M., King, A., Knights, A., Laird, G. K., Langford, C., Lawlor, S., Leongamornlert, D. A., Leversha, M., Lloyd, C., Lloyd, D. M., Lovell, J., Martin, S., Mashreghi-Mohammadi, M., Matthews, L., McLaren, S., McLay, K. E., McMurray, A., Milne, S., Nickerson, T., Nisbett, J., Nordsiek, G., Pearce, A. V., Peck, A. I., Porter, K. M., Pandian, R., Pelan, S., Phillimore, B., Povey, S., Ramsey, Y., Rand, V., Scharfe, M., Sehra, H. K., Shownkeen, R., Sims, S. K., Skuce, C. D., Smith, M., Steward, C. A., Swarbreck, D., Sycamore, N., Tester, J., Thorpe, A., Tracey, A., Tromans, A., Thomas, D. W., Wall, M., Wallis, J. M., West, A. P., Whitehead, S. L., Willey, D. L., Williams, S. A., Wilming, L., Wray, P. W., Young, L., Ashurst, J. L., Coulson, A., Blocker, H., Durbin, R., Sulston, J. E., Hubbard, T., Jackson, M. J., Bentley, D. R., Beck, S., Rogers, J., and Dunham, I.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): S. J. Humphray (corresponding author) [1]; K. Oliver [1]; A. R. Hunt [1]; R. W. Plumb [1]; J. E. Loveland [1]; K. L. Howe [1]; T. D. Andrews [1]; [...]

Published
2004
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5. The DNA sequence and analysis of human chromosome 6

Author

Mungall, A. J., Palmer, S. A., Sims, S. K., Edwards, C. A., Ashurst, J. L., Wilming, L., Jones, M. C., Horton, R., Hunt, S. E., Scott, C. E., Gilbert, J. G. R., Clamp, M. E., Bethel, G., Milne, S., Ainscough, R., Almeida, J. P., Ambrose, K. D., Andrews, T. D., Ashwell, R. I. S., Babbage, A. K., Bagguley, C. L., Bailey, J., Banerjee, R., Barker, D. J., Barlow, K. F., Bates, K., Beare, D. M., Beasley, H., Beasley, O., Bird, C. P., Blakey, S., Bray-Allen, S., Brook, J., Brown, A. J., Brown, J. Y., Burford, D. C., Burrill, W., Burton, J., Carder, C., Carter, N. P., Chapman, J. C., Clark, S. Y., Clark, G., Clee, C. M., Clegg, S., Cobley, V., Collier, R. E., Collins, J. E., Colman, L. K., Corby, N. R., Coville, G. J., Culley, K. M., Dhami, P., Davies, J., Dunn, M., Earthrowl, M. E., Ellington, A. E., Evans, K. A., Faulkner, L., Francis, M. D., Frankish, A., Frankland, J., French, L., Garner, P., Garnett, J., Ghori, M. J. R., Gilby, L. M., Gillson, C. J., Glithero, R. J., Grafham, D. V., Grant, M., Gribble, S., Griffiths, C., Griffiths, M., Hall, R., Halls, K. S., Hammond, S., Harley, J. L., Hart, E. A., Heath, P. D., Heathcott, R., Holmes, S. J., Howden, P. J., Howe, K. L., Howell, G. R., Huckle, E., Humphray, S. J., Humphries, M. D., Hunt, A. R., Johnson, C. M., Joy, A. A., Kay, M., Keenan, S. J., Kimberley, A. M., King, A., Laird, G. K., Langford, C., Lawlor, S., Leongamornlert, D. A., Leversha, M., Lloyd, C. R., Lloyd, D. M., Loveland, J. E., Lovell, J., Martin, S., Mashreghi-Mohammadi, M., Maslen, G. L., Matthews, L., McCann, O. T., McLaren, S. J., McLay, K., McMurray, A., Moore, M. J. F., Mullikin, J. C., Niblett, D., Nickerson, T., Novik, K. L., Oliver, K., Overton-Larty, E. K., Parker, A., Patel, R., Pearce, A. V., Peck, A. I., Phillimore, B., Phillips, S., Plumb, R. W., Porter, K. M., Ramsey, Y., Ranby, S. A., Rice, C. M., Ross, M. T., Searle, S. M., Sehra, H. K., Sheridan, E., Skuce, C. D., Smith, S., Smith, M., Spraggon, L., Squares, S. L., Steward, C. A., Sycamore, N., Tamlyn-Hall, G., Tester, J., Theaker, A. J., Thomas, D. W., Thorpe, A., Tracey, A., Tromans, A., Tubby, B., Wall, M., Wallis, J. M., West, A. P., White, S. S., Whitehead, S. L., Whittaker, H., Wild, A., Willey, D. J., Wilmer, T. E., Wood, J. M., Wray, P. W., Wyatt, J. C., Young, L., Younger, R. M., Bentley, D. R., Coulson, A., Durbin, R., Hubbard, T., Sulston, J. E., Dunham, I., Rogers, J., and Beck, S.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): A. J. Mungall (corresponding author) [1, 2]; S. A. Palmer [1]; S. K. Sims [1]; C. A. Edwards [1]; J. L. Ashurst [1]; L. Wilming [1]; M. C. Jones [...]

Published
2003
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6. Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13

Author

Hall, N., Pain, A., Berriman, M., Churcher, C., Harris, B., Harris, D., Mungall, K., Bowman, S., Atkin, R., Baker, S., Barron, A., Brooks, K., Buckee, C. O., Burrows, C., Cherevach, I., Chillingworth, C., Chillingworth, T., Christodoulou, Z., Clark, L., Clark, R., Corton, C., Cronin, A., Davies, R., Davis, P., Dear, P., Dearden, F., Doggett, J., Feltwell, T., Goble, A., Goodhead, I., Gwilliam, R., Hamlin, N., Hance, Z., Harper, D., Hauser, H., Hornsby, T., Holroyd, S., Horrocks, P., Humphray, S., Jagels, K., James, K. D., Johnson, D., Kerhornou, A., Knights, A., Konfortov, B., Kyes, S., Larke, N., Lawson, D., Lennard, N., Line, A., Maddison, M., McLean, J., Mooney, P., Moule, S., Murphy, L., Oliver, K., Ormond, D., Price, C., Quail, M. A., Rabbinowitsch, E., Rajandream, M.-A., Rutter, S., Rutherford, K. M., Sanders, M., Simmonds, M., Seeger, K., Sharp, S., Smith, R., Squares, R., Squares, S., Stevens, K., Taylor, K., Tivey, A., Unwin, L., Whitehead, S., Woodward, J., Sulston, J. E., Craig, A., Newbold, C., and Barrell, B. G.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): N. Hall (corresponding author) [1]; A. Pain [1]; M. Berriman [1]; C. Churcher [1]; B. Harris [1]; D. Harris [1]; K. Mungall [1]; S. Bowman [1, 2]; R. Atkin [...]

Published
2002
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7. The DNA sequence and comparative analysis of human chromosome 20

Author

Deloukas, P., Matthews, L. H., Ashurst, J., Burton, J., Gilbert, J. G. R., Jones, M., Stavrides, G., Almeida, J. P., Babbage, A. K., Bagguley, C. L., Bailey, J., Barlow, K. F., Bates, K. N., Beard, L. M., Beare, D. M., Beasley, O. P., Bird, C. P., Blakey, S. E., Bridgeman, A. M., Brown, A. J., Buck, D., Burrill, W., Butler, A. P., Carder, C., Carter, N. P., Chapman, J. C., Clamp, M., Clark, G., Clark, L. N., Clark, S. Y., Clee, C. M., Clegg, S., Cobley, V. E., Collier, R. E., Connor, R., Corby, N. R., Coulson, A., Coville, G. J., Deadman, R., Dhami, P., Dunn, M., Ellington, A. G., Frankland, J. A., Fraser, A., French, L., Garner, P., Grafham, D. V., Griffiths, C., Griffiths, M. N. D., Gwilliam, R., Hall, R. E., Hammond, S., Harley, J. L., Heath, P. D., Ho, S., Holden, J. L., Howden, P. J., Huckle, E., Hunt, A. R., Hunt, S. E., Jekosch, K., Johnson, C. M., Johnson, D., Kay, M. P., Kimberley, A. M., King, A., Knights, A., Laird, G. K., Lawlor, S., Lehvaslaiho, M. H., Leversha, M., Lloyd, C., Lloyd, D. M., Lovell, J. D., Marsh, V. L., Martin, S. L., McConnachie, L. J., McLay, K., McMurray, A. A., Milne, S., Mistry, D., Moore, M. J. F., Mullikin, J. C., Nickerson, T., Oliver, K., Parker, A., Patel, R., Pearce, T. A. V., Peck, A. I., Phillimore, B. J. C. T., Prathalingam, S. R., Plumb, R. W., Ramsay, H., Rice, C. M., Ross, M. T., Scott, C. E., Sehra, H. K., Shownkeen, R., Sims, S., Skuce, C. D., Smith, M. L., Soderlund, C., Steward, C. A., Sulston, J. E., Swann, M., Sycamore, N., Taylor, R., Tee, L., Thomas, D. W., Thorpe, A., Tracey, A., Tromans, A. C., Vaudin, M., Wall, M., Wallis, J. M., Whitehead, S. L., Whittaker, P., Willey, D. L., Williams, L., Williams, S. A., Wilming, L., Wray, P. W., Hubbard, T., Durbin, R. M., Bentley, D. R., Beck, S., and Rogers, J.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): The Wellcome Trust Sanger Institute ; P. Deloukas (corresponding author); L. H. Matthews; J. Ashurst; J. Burton; J. G. R. Gilbert; M. Jones; G. Stavrides; J. P. Almeida; A. [...]

Published
2001
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8. An SNP map of human chromosome 22

Author

Mullikin, J. C., Hunt, S. E., Cole, C. G., Mortimore, B. J., Rice, C. M., Burton, J., Matthews, L. H., Pavitt, R., Plumb, R. W., Sims, S. K., Ainscough, R. M. R., Attwood, J., Bailey, J. M., Barlow, K., Bruskiewich, R. M. M., Butcher, P. N., Carter, N. P., Chen, Y., Clee, C. M., Coggill, P. C., Davies, J., Davies, R. M., Dawson, E., Francis, M. D., Joy, A. A., Lamble, R. G., Langford, C. F., Macarthy, J., Mall, V., Moreland, A., Overton-Larty, E. K., Ross, M. T., Smith, L. C., Steward, C. A., Sulston, J. E., Tinsley, E. J., Turney, K. J., Willey, D. L., Wilson, G. D., McMurray, A. A., Dunham, I., Rogers, J., and Bentley, D. R.

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): J. C. Mullikin; S. E. Hunt; C. G. Cole; B. J. Mortimore; C. M. Rice; J. Burton; L. H. Matthews; R. Pavitt; R. W. Plumb; S. K. Sims; R. [...]

Published
2000
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9. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

Author

Cole, S. T., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S. V., Eiglmeier, K., Gas, S., Barry, III, C. E., Tekaia, F., Badcock, K., Basham, D., Brown, D., Chillingworth, T., Connor, R., Davies, R., Devlin, K., Feltwell, T., Gentles, S., Hamlin, N., Holroyd, S., Hornsby, T., Jagels, K., Krogh, A., McLean, J., Moule, S., Murphy, L., Oliver, K., Osborne, J., Quail, M. A., Rajandream, M.-A., Rogers, J., Rutter, S., Seeger, K., Skelton, J., Squares, R., Squares, S., Sulston, J. E., Taylor, K., Whitehead, S., and Barrell, B. G.

Published
1998
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11. The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X

Author

Bentley, D. R., Deloukas, P., Dunham, A., French, L., Gregory, S. G., Humphray, S. J., Mungall, A. J., Ross, M. T., Carter, N. P., Dunham, I., Scott, C. E., Ashcroft, K. J., Atkinson, A. L., Aubin, K., Beare, D. M., Bethel, G., Brady, N., Brook, J. C., Burford, D. C., Burrill, W. D., Burrows, C., Butler, A. P., Carder, C., Catanese, J. J., Clee, C. M., Clegg, S. M., Cobley, V., Coffey, A. J., Cole, C. G., Collins, J. E., Conquer, J. S., Cooper, R. A., Culley, K. M., Dawson, E., Dearden, F. L., Durbin, R. M., de Jong, P. J., Dhami, P. D., Earthrowl, M. E., Edwards, C. A., Evans, R. S., Gillson, C. J., Ghori, J., Green, L., Gwilliam, R., Halls, K. S., Hammond, S., Harper, G. L., Heathcott, R. W., Holden, J. L., Holloway, E., Hopkins, B. L., Howard, P. J., Howell, G. R., Huckle, E. J., Hughes, J., Hunt, P. J., Hunt, S. E., Izmajlowicz, M., Jones, C. A., Joseph, S. S., Laird, G., Langford, C. F., Lehvaslaiho, M. H., Leversha, M. A., McCann, O. T., McDonald, L. M., McDowall, J., Maslen, G. L., Mistry, D., Moschonas, N. K., Neocleous, V., Pearson, D. M., Phillips, K. J., Porter, K. M., Prathalingam, S. R., Ramsey, Y. H., Ranby, S. A., Rice, C. M., Rogers, J., Rogers, L. J., Sarafidou, T., Scott, D. J., Sharp, G. J., Shaw-Smith, C. J., Smink, L. J., Soderlund, C., Sotheran, E. C., Steingruher, H. E., Sulston, J. E., Taylor, A., Taylor, R. G., Thorpe, A. A., Tinsley, E., Warry, G. L., Whittaker, A., Whittaker, P., Williams, S. H., Wilmer, T. E., Wooster, R., and Wright, C. L.

Published
2001

12. The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum

Author

Bowman, S., Lawson, D., Basham, D., Brown, D., Chillingworth, T., Churcher, C. M., Craig, A., Davies, R. M., Devlin, K., Feltwell, T., Gentles, S., Gwilliam, R., Hamlin, N., Harris, D., Holroyd, S., Hornsby, T., Horrocks, P., Jagels, K., Jassal, B., Kyes, S., McLean, J., Moule, S., Mungall, K., Murphy, L., Oliver, K., Quail, M. A., Rajandream, M.-A., Rutter, S., Skelton, J., Squares, R., Squares, S., Sulston, J. E., Whitehead, S., Woodward, J. R., Newbold, C., and Barrell, B. G.

Published
1999

15. Erratum: The DNA sequence and biological annotation of human chromosome 1

Author

Gregory, S. G., primary, Barlow, K. F., additional, McLay, K. E., additional, Kaul, R., additional, Swarbreck, D., additional, Dunham, A., additional, Scott, C. E., additional, Howe, K. L., additional, Woodfine, K., additional, Spencer, C. C. A., additional, Jones, M. C., additional, Gillson, C., additional, Searle, S., additional, Zhou, Y., additional, Kokocinski, F., additional, McDonald, L., additional, Evans, R., additional, Phillips, K., additional, Atkinson, A., additional, Cooper, R., additional, Jones, C., additional, Hall, R. E., additional, Andrews, T. D., additional, Lloyd, C., additional, Ainscough, R., additional, Almeida, J. P., additional, Ambrose, K. D., additional, Anderson, F., additional, Andrew, R. W., additional, Ashwell, R. I. S., additional, Aubin, K., additional, Babbage, A. K., additional, Bagguley, C. L., additional, Bailey, J., additional, Banerjee, R., additional, Beasley, H., additional, Bethel, G., additional, Bird, C. P., additional, Bray-Allen, S., additional, Brown, J. Y., additional, Brown, A. J., additional, Bryant, S. P., additional, Buckley, D., additional, Burford, D. C., additional, Burrill, W. D. H., additional, Burton, J., additional, Bye, J., additional, Carder, C., additional, Chapman, J. C., additional, Clark, S. Y., additional, Clarke, G., additional, Clee, C., additional, Clegg, S. M., additional, Cobley, V., additional, Collier, R. E., additional, Corby, N., additional, Coville, G. J., additional, Davies, J., additional, Deadman, R., additional, Dhami, P., additional, Dovey, O., additional, Dunn, M., additional, Earthrowl, M., additional, Ellington, A. G., additional, Errington, H., additional, Faulkner, L. M., additional, Frankish, A., additional, Frankland, J., additional, French, L., additional, Garner, P., additional, Garnett, J., additional, Gay, L., additional, Ghori, M. R. J., additional, Gibson, R., additional, Gilby, L. M., additional, Gillett, W., additional, Glithero, R. J., additional, Grafham, D. V., additional, Gribble, S. M., additional, Griffiths, C., additional, Griffiths-Jones, S., additional, Grocock, R., additional, Hammond, S., additional, Harrison, E. S. I., additional, Hart, E., additional, Haugen, E., additional, Heath, P. D., additional, Holmes, S., additional, Holt, K., additional, Howden, P. J., additional, Hunt, A. R., additional, Hunt, S. E., additional, Hunter, G., additional, Isherwood, J., additional, James, R., additional, Johnson, C., additional, Johnson, D., additional, Joy, A., additional, Kay, M., additional, Kershaw, J. K., additional, Kibukawa, M., additional, Kimberley, A. M., additional, King, A., additional, Knights, A. J., additional, Lad, H., additional, Laird, G., additional, Langford, C. F., additional, Lawlor, S., additional, Leongamornlert, D. A., additional, Lloyd, D. M., additional, Loveland, J., additional, Lovell, J., additional, Lush, M. J., additional, Lyne, R., additional, Martin, S., additional, Mashreghi-Mohammadi, M., additional, Matthews, L., additional, Matthews, N. S. W., additional, McLaren, S., additional, Milne, S., additional, Mistry, S., additional, oore, M. J. F. M., additional, Nickerson, T., additional, O'Dell, C. N., additional, Oliver, K., additional, Palmeiri, A., additional, Palmer, S. A., additional, Pandian, R. D., additional, Parker, A., additional, Patel, D., additional, Pearce, A. V., additional, Peck, A. I., additional, Pelan, S., additional, Phelps, K., additional, Phillimore, B. J., additional, Plumb, R., additional, Porter, K. M., additional, Prigmore, E., additional, Rajan, J., additional, Raymond, C., additional, Rouse, G., additional, Saenphimmachak, C., additional, Sehra, H. K., additional, Sheridan, E., additional, Shownkeen, R., additional, Sims, S., additional, Skuce, C. D., additional, Smith, M., additional, Steward, C., additional, Subramanian, S., additional, Sycamore, N., additional, Tracey, A., additional, Tromans, A., additional, Van Helmond, Z., additional, Wall J. M. Wallis, M., additional, White, S., additional, Whitehead, S. L., additional, Wilkinson, J. E., additional, Willey, D. L., additional, Williams, H., additional, Wilming, L., additional, Wray, P. W., additional, Wu, Z., additional, Coulson, A., additional, Vaudin, M., additional, Sulston, J. E., additional, Durbin, R., additional, Hubbard, T., additional, Wooster, R., additional, Dunham, I., additional, Carter, N. P., additional, McVean, G., additional, Ross, M. T., additional, Harrow, J., additional, Olson, M. V., additional, Beck, S., additional, Rogers, J., additional, and Bentley, D. R., additional

Published
2006
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16. The DNA sequence of human chromosome 22

Author

Dunham, I., primary, Hunt, A. R., additional, Collins, J. E., additional, Bruskiewich, R., additional, Beare, D. M., additional, Clamp, M., additional, Smink, L. J., additional, Ainscough, R., additional, Almeida, J. P., additional, Babbage, A., additional, Bagguley, C., additional, Bailey, J., additional, Barlow, K., additional, Bates, K. N., additional, Beasley, O., additional, Bird, C. P., additional, Blakey, S., additional, Bridgeman, A. M., additional, Buck, D., additional, Burgess, J., additional, Burrill, W. D., additional, Burton, J., additional, Carder, C., additional, Carter, N. P., additional, Chen, Y., additional, Clark, G., additional, Clegg, S. M., additional, Cobley, V., additional, Cole, C. G., additional, Collier, R. E., additional, Connor, R. E., additional, Conroy, D., additional, Corby, N., additional, Coville, G. J., additional, Cox, A. V., additional, Davis, J., additional, Dawson, E., additional, Dhami, P. D., additional, Dockree, C., additional, Dodsworth, S. J., additional, Durbin, R. M., additional, Ellington, A., additional, Evans, K. L., additional, Fey, J. M., additional, Fleming, K., additional, French, L., additional, Garner, A. A., additional, Gilbert, J. G. R., additional, Goward, M. E., additional, Grafham, D., additional, Griffiths, M. N., additional, Hall, C., additional, Hall, R., additional, Hall-Tamlyn, G., additional, Heathcott, R. W., additional, Ho, S., additional, Holmes, S., additional, Hunt, S. E., additional, Jones, M. C., additional, Kershaw, J., additional, Kimberley, A., additional, King, A., additional, Laird, G. K., additional, Langford, C. F., additional, Leversha, M. A., additional, Lloyd, C., additional, Lloyd, D. M., additional, Martyn, I. D., additional, Mashreghi-Mohammadi, M., additional, Matthews, L., additional, McCann, O. T., additional, McClay, J., additional, McLaren, S., additional, McMurray, A. A., additional, Milne, S. A., additional, Mortimore, B. J., additional, Odell, C. N., additional, Pavitt, R., additional, Pearce, A. V., additional, Pearson, D., additional, Phillimore, B. J., additional, Phillips, S. H., additional, Plumb, R. W., additional, Ramsay, H., additional, Ramsey, Y., additional, Rogers, L., additional, Ross, M. T., additional, Scott, C. E., additional, Sehra, H. K., additional, Skuce, C. D., additional, Smalley, S., additional, Smith, M. L., additional, Soderlund, C., additional, Spragon, L., additional, Steward, C. A., additional, Sulston, J. E., additional, Swann, R. M., additional, Vaudin, M., additional, Wall, M., additional, Wallis, J. M., additional, Whiteley, M. N., additional, Willey, D., additional, Williams, L., additional, Williams, S., additional, Williamson, H., additional, Wilmer, T. E., additional, Wilming, L., additional, Wright, C. L., additional, Hubbard, T., additional, Bentley, D. R., additional, Beck, S., additional, Rogers, J., additional, Shimizu, N., additional, Minoshima, S., additional, Kawasaki, K., additional, Sasaki, T., additional, Asakawa, S., additional, Kudoh, J., additional, Shintani, A., additional, Shibuya, K., additional, Yoshizaki, Y., additional, Aoki, N., additional, Mitsuyama, S., additional, Roe, B. A., additional, Chen, F., additional, Chu, L., additional, Crabtree, J., additional, Deschamps, S., additional, Do, A., additional, Do, T., additional, Dorman, A., additional, Fang, F., additional, Fu, Y., additional, Hu, P., additional, Hua, A., additional, Kenton, S., additional, Lai, H., additional, Lao, H. I., additional, Lewis, J., additional, Lewis, S., additional, Lin, S.-P., additional, Loh, P., additional, Malaj, E., additional, Nguyen, T., additional, Pan, H., additional, Phan, S., additional, Qi, S., additional, Qian, Y., additional, Ray, L., additional, Ren, Q., additional, Shaull, S., additional, Sloan, D., additional, Song, L., additional, Wang, Q., additional, Wang, Y., additional, Wang, Z., additional, White, J., additional, Willingham, D., additional, Wu, H., additional, Yao, Z., additional, Zhan, M., additional, Zhang, G., additional, Chissoe, S., additional, Murray, J., additional, Miller, N., additional, Minx, P., additional, Fulton, R., additional, Johnson, D., additional, Bemis, G., additional, Bentley, D., additional, Bradshaw, H., additional, Bourne, S., additional, Cordes, M., additional, Du, Z., additional, Fulton, L., additional, Goela, D., additional, Graves, T., additional, Hawkins, J., additional, Hinds, K., additional, Kemp, K., additional, Latreille, P., additional, Layman, D., additional, Ozersky, P., additional, Rohlfing, T., additional, Scheet, P., additional, Walker, C., additional, Wamsley, A., additional, Wohldmann, P., additional, Pepin, K., additional, Nelson, J., additional, Korf, I., additional, Bedell, J. A., additional, Hillier, L., additional, Mardis, E., additional, Waterston, R., additional, Wilson, R., additional, Emanuel, B. S., additional, Shaikh, T., additional, Kurahashi, H., additional, Saitta, S., additional, Budarf, M. L., additional, McDermid, H. E., additional, Johnson, A., additional, Wong, A. C. C., additional, Morrow, B. E., additional, Edelmann, L., additional, Kim, U. J., additional, Shizuya, H., additional, Simon, M. I., additional, Dumanski, J. P., additional, Peyrard, M., additional, Kedra, D., additional, Seroussi, E., additional, Fransson, I., additional, Tapia, I., additional, Bruder, C. E., additional, and O'Brien, K. P., additional

Published
1999
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19. Short-insert libraries as a method of problem solving in genome sequencing.

Author

McMurray, A A, Sulston, J E, and Quail, M A

Abstract

As the Human Genome Project moves into its sequencing phase, a serious problem has arisen. The same problem has been increasingly vexing in the closing phase of the Caenorhabditis elegans project. The difficulty lies in sequencing efficiently through certain regions in which the templates (DNA substrates for the sequencing process) form complex folded secondary structures that are inaccessible to the enzymes. The solution, however, is simply to break them up. Specifically, the offending fragments are sonicated heavily and recloned, as much smaller fragments, into pUC vector. The sequences obtained from the resulting library can subsequently be assembled, free from the effects of secondary structure, to produce high-quality, complete sequence. Because of the success and simplicity of this procedure, we have begun to use it for the sequencing of all regions in which standard primer walking has been at all difficult.

Published
1998

25. Caenorhabditis elegans levamisole resistance genes lev-1, unc-29, and unc-38 encode functional nicotinic acetylcholine receptor subunits.

Author

Fleming JT, Squire MD, Barnes TM, Tornoe C, Matsuda K, Ahnn J, Fire A, Sulston JE, Barnard EA, Sattelle DB, and Lewis JA

Subjects
Amino Acid Sequence, Animals, Cloning, Molecular, Molecular Sequence Data, Phenotype, Xenopus, Genes genetics, Mutation genetics, Receptors, Nicotinic genetics
Abstract

We show that three of the eleven genes of the nematode Caenorhabditis elegans that mediate resistance to the nematocide levamisole and to other cholinergic agonists encode nicotinic acetylcholine receptor (nAChR) subunits. unc-38 encodes an alpha subunit while lev-1 and unc-29 encode non-alpha subunits. The nematode nAChR subunits show conservation of many mammalian nAChR sequence features, implying an ancient evolutionary origin of nAChR proteins. Expression in Xenopus oocytes of combinations of these subunits that include the unc-38 alpha subunit results in levamisole-induced currents that are suppressed by the nAChR antagonists mecamylamine, neosurugatoxin, and d-tubocurarine but not alpha-bungarotoxin. The mutant phenotypes reveal that unc-38 and unc-29 subunits are necessary for nAChR function, whereas the lev-1 subunit is not. An UNC-29-GFP fusion shows that UNC-29 is expressed in body and head muscles. Two dominant mutations of lev-1 result in a single amino acid substitution or addition in or near transmembrane domain 2, a region important to ion channel conductance and desensitization. The identification of viable nAChR mutants in C. elegans provides an advantageous system in which receptor expression and synaptic targeting can be manipulated and studied in vivo.

Published
1997

26. Sequence variation of the human Y chromosome.

Author

Whitfield LS, Sulston JE, and Goodfellow PN

Subjects
Animals, DNA, Mitochondrial genetics, Humans, Male, Pan troglodytes, Genetic Variation, Y Chromosome
Abstract

We have generated over 100 kilobases of sequence from the nonrecombining portion of the Y chromosomes from five humans and one common chimpanzee. The human subjects were chosen to match the earliest branches of the human mitochondrial tree. The survey of 18.3 kilobases from each human detected only three sites at which substitutions were present, whereas the human and chimpanzee sequences showed 1.3% divergence. The coalescence time estimated from our Y chromosome sample is more recent than that of the mitochondrial genome. A recent coalescence time for the Y chromosome could have been caused by the selected sweep of an advantageous Y chromosome or extensive migration of human males.

Published
1995
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28. Chromatin diminution and a chromosomal mechanism of sexual differentiation in Strongyloides papillosus.

Author

Albertson DG, Nwaorgu OC, and Sulston JE

Subjects
Animals, Karyotyping, Mitosis, Nematode Infections genetics, Parthenogenesis, Rabbits, Chromosomes ultrastructure, Sex Differentiation, Strongyloidea genetics
Abstract

Eggs obtained from feces of rabbits infected with Strongyloides papillosus were squashed and the karyotypes were determined. They contained cells with either two long and two medium sized chromosomes (2L2M), or one long, three medium and one short chromosome (L3MS). Two types of parasitic female gonad could be distinguished on the basis of oocyte chromosome morphology at prometaphase of the maturation division. All the oocytes in a gonad contained either two upaired long chromosomes and two unpaired medium sized chromosomes, or two unpaired medium sized chromosomes and two unpaired chromosomes segmented into beads in one region. At the maturation division in mitotic parthenogenesis the beads appear to be lost from one of the chromosomes. This generates a medium sized and a shorter chromosome, which together with the undiminished chromosomes make up the L3MS karyotype. Animals with beaded oocyte chromosomes lay eggs that develop into males. It is suggested that males are heteromorphic for the long homologue due to chromatin diminution, that occurs in the maturation division of mitotic parthenogenesis.

Published
1979
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29. The Caenorhabditis elegans male: postembryonic development of nongonadal structures.

Author

Sulston JE, Albertson DG, and Thomson JN

Subjects
Animals, Cell Survival, Cloaca growth & development, Digestive System growth & development, Disorders of Sex Development, Ganglia growth & development, Male, Microscopy, Electron, Muscle Development, Skin growth & development, Tail cytology, Tail innervation, Tail ultrastructure, Vas Deferens growth & development, Caenorhabditis growth & development, Tail growth & development
Published
1980
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30. The rDNA of C. elegans: sequence and structure.

Author

Ellis RE, Sulston JE, and Coulson AR

Subjects
Base Sequence, Biological Evolution, Cloning, Molecular, Genes, Nucleic Acid Conformation, Sequence Homology, Nucleic Acid, Caenorhabditis genetics, DNA, Ribosomal genetics, RNA, Ribosomal genetics
Abstract

We have sequenced one complete rDNA tandem repeat from the nematode C. elegans. By comparative analysis we derive secondary structures for the 18s, 5.8s, and 26s rRNA molecules, and comment on other important features of the sequence. We also present the sequence of a junction between the rDNA and non-ribosomal DNA. Finally, we use our data to quantify the evolutionary relationships among several organisms currently studied in developmental biology.

Published
1986
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31. Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency.

Author

Gibson TJ, Coulson AR, Sulston JE, and Little PF

Subjects
Animals, Caenorhabditis genetics, Cell Division, Escherichia coli genetics, Promoter Regions, Genetic, Cloning, Molecular methods, Cosmids, DNA Replication, Genes, Regulator, Genetic Vectors, Terminator Regions, Genetic
Abstract

Transcription terminators have been included in a phage-lambda-replicon-based cosmid vector, Lorist2, to insulate vector genes against transcriptional interference from cloned insert DNA. DNA yields of recombinant clones containing Escherichia coli genomic DNA inserts are more even for Lorist2 than with its progenitor LoristB. However, the terminators provide only a partial reduction in the over-representation of r X DNA-containing clones generally observed in cosmid libraries of Caenorhabditis elegans DNA, suggesting that causes other than transcriptional readthrough into the vector contribute to this problem.

Published
1987
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33. Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans.

Author

Horvitz HR and Sulston JE

Subjects
Animals, Cell Division, Crosses, Genetic, Genes, Recessive, Mutation, Phenotype, Caenorhabditis genetics, Germ Layers physiology
Abstract

Twenty-four mutants that alter the normally invariant post-embryonic cell lineages of the nematode Caenorhabditis elegans have been isolated and genetically characterized. In some of these mutants, cell divisions fail that occur in wild-type animals; in other mutants, cells divide that do not normally do so. The mutants differ in the specificities of their defects, so that it is possible to identify mutations that affect some cell lineages but not others. These mutants define 14 complementation groups, which have been mapped. The abnormal phenotype of most of the cell-lineage mutants results from a single recessive mutation; however, the excessive cell divisions characteristic of one strain, CB1322, require the presence of two unlinked recessive mutations. All 24 cell-lineage mutants display incomplete penetrance and/or variable expressivity. Three of the mutants are suppressed by pleiotropic suppressors believed to be specific for null alleles, suggesting that their phenotypes result from the complete absence of gene activity.

Published
1980
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34. Mutations affecting programmed cell deaths in the nematode Caenorhabditis elegans.

Author

Hedgecock EM, Sulston JE, and Thomson JN

Subjects
Animals, Autophagy, Caenorhabditis genetics, DNA metabolism, Female, Male, Microscopy, Electron, Caenorhabditis growth & development, Cell Survival, Mutation
Abstract

Mutations in two nonessential genes specifically block the phagocytosis of cells programmed to die during development. With few exceptions, these cells still die, suggesting that, in nematodes, engulfment is not necessary for most programmed deaths. Instead, these deaths appear to occur by cell suicide.

Published
1983
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35. The neural circuit for touch sensitivity in Caenorhabditis elegans.

Author

Chalfie M, Sulston JE, White JG, Southgate E, Thomson JN, and Brenner S

Subjects
Animals, Caenorhabditis, Interneurons physiology, Lasers, Mechanoreceptors physiology, Motor Neurons physiology, Movement, Muscle Contraction, Neural Pathways physiology, Synapses physiology, Nervous System Physiological Phenomena, Touch physiology
Abstract

The neural pathways for touch-induced movement in Caenorhabditis elegans contain six touch receptors, five pairs of interneurons, and 69 motor neurons. The synaptic relationships among these cells have been deduced from reconstructions from serial section electron micrographs, and the roles of the cells were assessed by examining the behavior of animals after selective killing of precursors of the cells by laser microsurgery. This analysis revealed that there are two pathways for touch-mediated movement for anterior touch (through the AVD and AVB interneurons) and a single pathway for posterior touch (via the PVC interneurons). The anterior touch circuitry changes in two ways as the animal matures. First, there is the formation of a neural network of touch cells as the three anterior touch cells become coupled by gap junctions. Second, there is the addition of the AVB pathway to the pre-existing AVD pathway. The touch cells also synapse onto many cells that are probably not involved in the generation of movement. Such synapses suggest that stimulation of these receptors may modify a number of behaviors.

Published
1985

36. Post-embryonic cell lineages of the nematode, Caenorhabditis elegans.

Author

Sulston JE and Horvitz HR

Subjects
Animals, Cell Count, Cell Division, Cell Nucleus ultrastructure, Cell Survival, Disorders of Sex Development, Male, Nematoda anatomy & histology, Organ Specificity, Sex Differentiation, Species Specificity, Cell Differentiation, Embryonic Induction, Nematoda physiology
Published
1977
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37. Serotonin and octopamine in the nematode Caenorhabditis elegans.

Author

Horvitz HR, Chalfie M, Trent C, Sulston JE, and Evans PD

Subjects
Age Factors, Animals, Behavior, Animal physiology, Female, Ovulation drug effects, Temperature, Caenorhabditis physiology, Octopamine physiology, Serotonin physiology
Abstract

The biogenic amines serotonin and octopamine are present in the nematode Caenorhabditis elegans. Serotonin, detected histochemically in whole mounts, is localized in two pharyngeal neurons that appear to be neurosecretory. Octopamine, identified radioenzymatically in crude extracts, probably is also localized in a few neurons. Exogenous serotonin and octopamine elicit specific and opposite behavioral responses in Caenorhabditis elegans, suggesting that these compounds function physiologically as antagonists.

Published
1982
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38. Induction of neuronal branching in Caenorhabditis elegans.

Author

Chalfie M, Thomson JN, and Sulston JE

Subjects
Animals, Caenorhabditis physiology, Insecta growth & development, Insecta physiology, Movement, Nervous System Physiological Phenomena, Neurons, Afferent physiology, Synapses physiology, Touch physiology, Caenorhabditis growth & development, Nervous System growth & development
Abstract

The two postembryonic touch receptor neurons in the nematode Caenorhabditis elegans arise from essentially identical cell lineages and have the same ultrastructural features. The cells are found in different positions in the animal, however, and differ in neuronal branching, connectivity, and function. These structural and functional differences are not seen when cells are placed in similar positions by mutation or laser-induced damage. Thus, some, but probably not all, of the differentiated properties of these cells are a consequence of their cellular environment.

Published
1983
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39. Preparation of large numbers of plasmid DNA samples in microtiter plates by the alkaline lysis method.

Author

Gibson TJ and Sulston JE

Subjects
Cosmids, Escherichia coli genetics, Genetic Engineering methods, Hydrogen-Ion Concentration, DNA, Bacterial isolation & purification, Plasmids
Abstract

A protocol is described for the growth and preparation of plasmid DNAs from small culture volumes (250 microliters) and utilizing standard 96-well plates. Several hundred plasmids can be prepared simultaneously, yielding sufficient DNA for subsequent analysis by restriction digestion and gel electrophoresis. This protocol may be useful for rapid screening of clones arising in recombinant DNA work such as site-directed mutagenesis, oligonucleotide cassette cloning, deletion analysis, etc. The technique was initially developed to meet our requirement to provide large numbers of cosmid DNAs for restriction enzyme fingerprint analyses in genome mapping projects.

Published
1987
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40. Mutations that lead to reiterations in the cell lineages of C. elegans.

Author

Chalfie M, Horvitz HR, and Sulston JE

Subjects
Animals, Caenorhabditis physiology, Caenorhabditis ultrastructure, Cell Division, Embryo, Nonmammalian physiology, Larva physiology, Larva ultrastructure, Microscopy, Electron, Caenorhabditis genetics, Genes, Mutation
Abstract

Cells in the nematode Caenorhabditis elegans arise from invariant cell lineages. Mutations in two genes, unc-86 and lin-4, alter multiple and mutually exclusive sets of these lineages. In these mutants, particular cells repeat division patterns normally associated with their parental or grandparental progenitors. The effects of unc-86 are highly specific, altering in equivalent ways the lineages of three post-embryonic neuroblasts that in the wild-type undergo similar division patterns. The effects of lin-4 are more varied, resulting in a number of types of lineage reiterations as well as in supernumerary molts and the continued synthesis of larval-specific cuticle. The reiteration of a given cell division or pattern of cell divisions leads to the repeated generation of cells indistinguishable (by both light and electron microscopy) from those produced after the same division or pattern of cell divisions in the wild-type. This correlation between lineage history and cell fate suggests that in C. elegans a particular sequence of cell divisions may be necessary for the generation of a particular cell type. Reiterative lineages, often referred to as stem cell lineages, may be basic to the development of nematodes and other organisms. We suggest that the wild-type unc-86 and lin-4 genes act to modify latent reiterative cell lineages, which are revealed when the activity of one of these genes is eliminated.

Published
1981
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41. Genomic organization of major sperm protein genes and pseudogenes in the nematode Caenorhabditis elegans.

Author

Ward S, Burke DJ, Sulston JE, Coulson AR, Albertson DG, Ammons D, Klass M, and Hogan E

Subjects
Animals, Base Sequence, Chromosome Mapping, Cosmids, DNA genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Caenorhabditis genetics, Genes, Nuclear Proteins genetics, Pseudogenes
Abstract

The major sperm proteins (MSPs) are a family of closely related, small, basic proteins comprising 15% of the protein in Caenorhabditis elegans sperm. They are encoded by a multigene family of more than 50 genes, including many pseudogenes. MSP gene transcription occurs only in late primary spermatocytes. In order to study the genomic organization of transcribed MSP genes, probes specific for the 3' untranslated regions of sequenced cDNA clones were used to isolate transcribed genes from genomic libraries. These and other clones of MSP genes were located in overlapping cosmid clones by DNA fingerprinting. These cosmids were aligned with the genetic map by overlap with known genes or in-situ hybridization to chromosomes. Of 40 MSP genes identified, 37, including all those known to be transcribed, are organized into six clusters composed of 3 to 13 genes each. Within each cluster, MSP genes are not in tandem but are separated by at least several thousand bases of DNA. Pseudogenes are interspersed among functional genes. Genes with similar 3' untranslated sequences are in the same cluster. The six MSP clusters are confined to only three chromosomal loci; one on the left arm of chromosome II and two near the middle of chromosome IV. Additional sperm-specific genes are located in one cluster of MSP genes on chromosome IV. The multiplicity of MSP genes appears to be a mechanism for enhancing MSP synthesis in spermatocytes, and the loose clustering of genes could be a result of the mechanism of gene duplication or could play a role in regulation.

Published
1988
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43. The embryonic cell lineage of the nematode Caenorhabditis elegans.

Author

Sulston JE, Schierenberg E, White JG, and Thomson JN

Subjects
Animals, Cell Communication, Cell Differentiation, Cell Division, Cell Movement, Cell Survival, Digestive System cytology, Female, Gastrula physiology, Gonads cytology, Larva cytology, Male, Mesoderm cytology, Microscopy, Electron, Muscles cytology, Neurons cytology, Caenorhabditis embryology, Zygote cytology
Abstract

The embryonic cell lineage of Caenorhabditis elegans has been traced from zygote to newly hatched larva, with the result that the entire cell lineage of this organism is now known. During embryogenesis 671 cells are generated; in the hermaphrodite 113 of these (in the male 111) undergo programmed death and the remainder either differentiate terminally or become postembryonic blast cells. The embryonic lineage is highly invariant, as are the fates of the cells to which it gives rise. In spite of the fixed relationship between cell ancestry and cell fate, the correlation between them lacks much obvious pattern. Thus, although most neurons arise from the embryonic ectoderm, some are produced by the mesoderm and a few are sisters to muscles; again, lineal boundaries do not necessarily coincide with functional boundaries. Nevertheless, cell ablation experiments (as well as previous cell isolation experiments) demonstrate substantial cell autonomy in at least some sections of embryogenesis. We conclude that the cell lineage itself, complex as it is, plays an important role in determining cell fate. We discuss the origin of the repeat units (partial segments) in the body wall, the generation of the various orders of symmetry, the analysis of the lineage in terms of sublineages, and evolutionary implications.

Published
1983
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45. Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans.

Author

Sulston JE and White JG

Subjects
Animals, Caenorhabditis cytology, Cell Differentiation radiation effects, Cell Survival radiation effects, Cloaca cytology, Digestive System cytology, Disorders of Sex Development, Female, Ganglia cytology, Lasers, Male, Sense Organs cytology, Skin cytology, Vas Deferens cytology, Vulva cytology, Caenorhabditis growth & development
Published
1980
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48. Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae.

Author

Rubin GM and Sulston JE

Subjects
Centrifugation, Density Gradient, DNA analysis, Electrophoresis, Polyacrylamide Gel, Nitrogen Isotopes, Nucleic Acid Hybridization, Phosphorus Radioisotopes, RNA, Ribosomal analysis, RNA, Transfer metabolism, Ribonucleases, Saccharomyces cerevisiae analysis, Spectrophotometry, Ultraviolet, Ultracentrifugation, DNA metabolism, RNA, Ribosomal metabolism, Saccharomyces cerevisiae metabolism, Transcription, Genetic
Published
1973
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