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Indonesia is listed as a region with high marine biodiversity, especially when considering the three large tropical ecosystems: seagrass beds, mangroves and coral reefs. It is likely that the biodiversity of diatoms in this area is also high. Samples for this research were taken from the remote area of Bawean Island and Tomini Gulf in Central Sulawesi. In this research, we explored marine benthic diatoms from coral reef areas and presented two new genera: Paracatenula and Wallaceago, and seven new species: Paracatenula porostriata sp. nov., Wallaceago porostriatus sp. nov., Catenula boyanensis sp. nov., C. komodensis sp. nov., C. decusa sp. nov., C. densestriata sp. nov., and Catenulopsis baweana sp. nov. The new genus Paracatenula is characterized by its perforated cingulum, and the genus Wallaceago is distinguished by its proximal and apical raphe ends bent to the ventral side and the presence of a stauros in the mantle.

During a survey of the Indonesian diatoms, five Luticola D.G.Mann taxa that could not be identified, based on the available literature were discovered. Based on light microscopy, scanning electron microscope observations and comparisons with similar taxa, all of them are described as new species. All taxa were found on mosses growing on tree trunks and concrete on the islands of Banda Besar and Seram and from spring on Java Island. Luticola insularis sp. nov. is most similar to L. aequatorialis and L. simplex, but it can easily be distinguished from both taxa, based on the lower striae density, the narrower valves and the well-developed silica ridges on the valve face/mantle junction. Luticola bandanensis sp. nov. resembles L. frequentissima, but they can be easily distinguished, based on their valve widths and the direction of the grooves located on the distal and proximal raphe endings. Luticola elliptica sp. nov. is most similar to L. sparsipunctata, L. tenuis and L. bryophila. Amongst all the species compared, L. elliptica sp. nov. is the only one with a highly asymmetrical central area, with the isolated pore located on the wider side. Luticola malukuana sp. nov. shares similarities with L. dismutica and L. areolata, but it has a notably higher stria density. From L. areolata, it can also be separated by the morphology of striae and the lack of ghost areolae in the central area. Luticola poliporea sp. nov. is unique in the whole genus due to the presence of multiple isolated pores.

Diatom, Bacillariophyceae, has an important role in environmental support because these algae contribute to 25% of the oxygen supply globally and each year represent 40% of primary production by fixed carbon using photosynthesis. Diatom samples were obtained from 3 different sites in around NRPS. Cleaning diatoms analyzed using SEM for describe ultrastructure in diatom valves. Three dominant diatoms Cocconeidaceae and Bacillariaceae from the south Java Sea (Indian Ocean). Basic information regarding the diversity of diatoms, and the success of photoautotrophic micro-algae that can be found both in marine and freshwater habitats, is very important to describe the role of each taxon from an ecology perspective in the future. Samples are taken from the substrate of 3 stations in Nature Reserve Pulau Sempu and prepared in the laboratory for scanning electron microscopy photographs. Clear and detailed features diatom frustule of SEM photograph reveal a schild diatom (Cocconeis) and panduriform diatom (Psammodictyon) are dominating taxa in NRSP areas. Keywords: Hard coral Cocconeis Psammodictyon Pulau Sempu Diatom

Samples from coastal tropical waters of Central Sulawesi, Bangka Island and Bawean Island in Indonesia and from the Great Barrier Reef at Fitzroy Island in Queensland, Australia were analysed for species composition of diatom assemblages with a focus on Olifantiella. Whereas samples from Fitzroy Island littoral in Australia retrieved only one species of Olifantiella, in Poso Bay, Indonesia, we observed at least six species. All established taxa were documented with light (LM) and scanning electron microscope (SEM) and principal component analysis (PCA) analysis was used to compare the species, based on the basic valve parameters of length, width, length to width ratio and striae density. A new species of the genus Olifantiella, O. gondwanensis is described from Australia. In addition, we showed the distinct nature of O. pilosella var. rhizophorae permitting to species status. Particular attention is placed on girdle bands in this genus.

Background and Aim: Coronavirus disease-2019 (COVID-19) impacts increasing the use of disinfectants (benzalkonium chloride), which indirectly accumulate in water. The disinfectant accumulation in waters has been studied, but there has been no study of its impact on aquatic commodities, especially fish with a high sensitivity, one of which is goldfish (Carassius auratus). Benzalkonium chloride can potentially affect several body proteins, including the cytoskeleton, nervous and endocrine systems, and fish physiology. This study aimed to determine the impact of benzalkonium chloride as a disinfectant on the level of color brightness, growth, gill histopathology, and mortality in goldfish. This investigation provides input into the impact of using disinfectants to prevent COVID-19 on aquatic commodities. Materials and Methods: This study utilized goldfish specimens sourced from Tulungagung, East Java, Indonesia. The experiment involved different concentration levels of benzalkonium chloride: (T1) 0 mg/L, (T2) 0.03 mg/L, (T3) 0.06 mg/L, (T4) 0.09 mg/L, and (T5) 0.12 mg/L. The research data were subjected to an analysis of variance for analysis. In cases where significant differences were observed, Duncan’s test was conducted for color brightness, growth, and mortality data. Furthermore, if the gill histopathological data yielded significant differences, additional tests were applied (Kruskal–Wallis and Mann–Whitney test). Results: The findings of this study demonstrated significant differences (p < 0.05) in the level of color brightness, growth, gill histopathology, and mortality in goldfish in response to varying concentrations of benzalkonium chloride. The relationship between the length and weight of the goldfish was analyzed using regression coefficients (b values), which were determined as 4.86, –0.04, –0.2, 0.8, and –0.07, respectively. Notably, the brightness level in the T2 group exhibited positive color results with a hue value of 11.55°, while optimal growth was observed in the T4 group, as evidenced by b value of 0.8. The gill histopathological data showed significant differences (p < 0.05). The scoring of histopathological damage in the goldfish gills ranged from 0 to 10, with higher scores indicating more severe damage. The highest total score of 10 was observed in the T5 group exposed to a concentration of 0.12 mg/L, resulting in an 85% mortality rate. This indicates that benzalkonium chloride, with its toxic compounds, can disrupt the respiratory system of fish and lead to death. Conclusion: The effects of benzalkonium chloride were evident even at a concentration of 0.03 mg/L. With increasing concentration, there was an increase in mortality rate, a decrease in growth, and a rise in histopathological damage to the gills. These findings highlight the negative impact of using conventional disinfectants on water and its organisms, emphasizing the need for further research on environmentally friendly alternatives.

This research analysed the availability of phytoplankton and the growth rate of Vannamei shrimp in relation to water quality changes. The research was carried out in February–March 2021 for a half cycle of shrimp cultivation in two ponds of the Brackish Water Fish Culture Probolinggo Laboratory in Probolinggo, East Java, Indonesia. The research used a descriptive method and included a survey. Sampling was made every two weeks for two months. Nine parameters were measured and ten shrimps were taken for a specific growth rate ( SGR) measurement once per sampling. Data were analysed using the principal component analysis (PCA) and canonical correspondence analysis (CCA). Secondary data of water quality were added for the PCA. The results show that the phytoplankton found in the first pond consisted of Chlorophyta, Chrysophyta, and Cyanophyta, whereas the phytoplankton in the other pond included Chlorophyta, Chrysophyta, Cyanophyta, and Dinophyta. The abundance of phytoplankton ranged from 12–80∙10 3 cell∙cm –3, which indicated eutrophic waters. The PCA demonstrated that pH, nitrate, and total organic matter (TOM) significantly influenced phytoplankton abundance in the pond. In addition, water quality parameters, such as temperature, transparency, salinity, nitrite and phosphate levels, were tolerable in both ponds for the growth of shrimps. However, the level of pH was lower than the aquaculture quality standard, whereas those of nitrate, ammonia, and TOM were higher. The growth rate of Vannamei shrimp increased by 0.76–7.34%∙day –1.

The exact number of coral species in Indonesia is not unclear yet, in 2002 the coral taxonomist, JEN Veron, stated 590 species, and it was supported by an Indonesian coral taxonomist, Suharsono, eight years later. The current coral species data is 605 but it is from Coral Triangle Region area (CT). The eastern of Indonesia, which is located in the Coral Triangle Region, has received a lot of attention from coral researchers, due to coral species that have been described. On the other hand, the coral reef from the western part of Indonesia, including in the East Kalimantan, has not been explored much in recent times. Through the ecoregion view, E. Kalimantan region is clustered into ecoregion 43 (Sulu Sea) which has 540 coral species. Miang Island is one of the inhabited islands from E. Kalimantan, and has an unexplored coral reef ecosystem. The method had been used in this research is descriptive, which uses photos of live corals to describe colony characteristics corals. The results of the study have described 36 species of coral and several invertebrates that make up coral reefs on Miang Island. Keywords: Hard coral Acropora Porites Identification Pulau Miang

The Community Partnership Program (PKM) aims to conduct training on microalgae culture and water quality management in aquaculture ponds as an effort to improve the quality of natural feed for shrimp seeds. This activity was carried out in the Tuban Barokah Benur group in 2021. A cultivation experiment of three types of microalgae was carried out and the species was given as a natural feed for shrimp seeds. Microalgae Tetraselmis sp., Dunaliella sp., and Chlorella sp. were cultured for 7 days and tested as shrimp seed feed. The results showed that the percentage of survival of shrimp seed was highest in the containers given byTetraselmis sp. (32%). Furthermore, microalgae culture assistance activities in the Benur Barokah group used the microalgae Tetraselmis sp. carried out in Situ on a small scale. In addition, water quality tool measurement is also provided to help monitor the water quality of the tank regularly. The questionnaire results showed a positive response to training activities from Benur Barokah partners covering six aspects with a good response of 50-90%.

The global pandemic of COVID-19 caused by SARS-CoV-2 has caused more than 400 million infections with more than 5.7 million deaths worldwide, and the number of validated therapies from natural products for treating coronavirus infections needs to be increased. Therefore, the virtual screening of bioactive compounds from natural products based on computational methods could be an interesting strategy. Among many sources of bioactive natural products, compounds from marine organisms, particularly microalgae and cyanobacteria, can be potential antiviral agents. The present study investigates bioactive antiviral compounds from microalgae and cyanobacteria as a potential inhibitor of SARS-CoV-2 by targeting Angiotensin-Converting Enzyme II (ACE2) using integrated in silico and in vitro approaches. Our in silico analysis demonstrates that C-Phycocyanin (CPC) can potentially inhibit the binding of ACE2 receptor and SARS-CoV-2 with the docking score of −9.7 kcal mol−1. This score is relatively more favorable than the native ligand on ACE2 receptor. Molecular dynamics simulation also reveals the stability interaction between both CPC and ACE2 receptor with a root mean square deviation (RMSD) value of 1.5 Å. Additionally, our in vitro analysis using the surface plasmon resonance (SPR) method shows that CPC has a high affinity for ACE2 with a binding affinity range from 5 to 125 µM, with KD 3.37 nM. This study could serve as a reference to design microalgae- or cyanobacteria-based antiviral drugs for prophylaxis in SARS-CoV-2 infections.

Turtles are one of the reptile animals that can migrate long distances along the Indian Ocean, Pacific Ocean and Southeast Asia. The purpose of turtle migration is to mate, find spawning locations and to find food. Sea turtles have an important role in maintaining the balance of the marine ecosystem. However, the high utilization of sea turtles by humans causes the turtle population to decline. One of the conservation sites that help the government program is in Serangan, Denpasar Bali, namely the TCEC (Turtle Conservation and Education Center), where there are several types of turtles such as the lekang turtle, hawksbill and green turtle. Data collection techniques in this study used observation methods including beach monitoring, relocation of turtle eggs to semi-natural hatchery hatching, hatchling and turtle nesting as well as measurement of turtle length and width carapase curve (CCL/CCW), sand temperature, nest depth, nest diameter, nest distance and nest calculation of the number of eggs to see the success of hatching. The results are turtle conservation activities, ranging from turtle maintenance, hatching of turtle eggs, relocation of eggs, and maintenance of hatchlings to release turtles. The effectiveness given by TCEC to manage and preserve the existence of sea turtles in Indonesia, especially in the Bali region is to provide awareness to the community by not consuming eggs and turtle meat.

The mangrove forest in Brebes is one of the mangrove areas that were degraded due to abrasion and excessive logging in the North Coast of Java Island, Indonesia. This research aimed to analyze macrozoobenthos community structure in the mangrove forest that has not been documented. Surveys were carried out in the rainy season. In this study, a total of 10 sampling locations were divided into two different groups that represent “control” (station) and “disturbed” sites. The main structural parameters of the macrozoobenthos identified at each station were specific richness S (number of species), abundance N (number of individuals.m-2), the Shannon-Wiener index N1. A total of 346 specimens were counted from the two different stations (“control” and “disturbed”). These specimens were belonged to five animal classes, namely, Bivalvia, Gastropod, Malacostraca, Polychaeta, Cephalopoda. Pairwise comparison of the site groups with one-way analysis of similarity (ANOSIM) was not significant for between “control” and “disturbed” groups (p=0.062), where the average dissimilarity between the two stations was 88.42%. This result provides macrozoobenthos diversity and ecological information that may contribute to further conservation management in the mangrove forest in Brebes, Indonesia.

Pakan yang mahal dan teknologi yang masih tradisional menjadi masalah yang serius bagi pembudidaya udang vanamei di Desa Temaji. Salah satu alternatif yang ditawarkan yaitu melakukan pembuatan pellet secara mandiri dalam sistem budidaya semi intensif. Tujuan utama pelaksanaan Doktor Mengabdi (DM) yaitu mengembangkan keterampilan pembudidaya dalam memproduksi pakan mandiri skala kecil dengan menggunakan mesin pellet sederhana guna mengurangi biaya operasional dari cost pakan. Selain itu, tujuannya adalah untuk meningkatkan keterampilan mitra dalam pengukuran kualitas air sehingga produksi hasil budidaya dapat meningkat dan secara tidak langsung dapat meningkatkan pendapatan dan kesejahteraan masyarakat pembudidaya. Hasil kegiatan ini yaitu diperolehnya produk pellet MINIPRO yang dikemas sendiri dan berdasarkan kalkulasi ternyata hasil pellet yang diproduksi jauh lebih murah dibandingkan dengan pellet yang dibeli. Kegiatan ini juga diakhiri dengan pemberian kuesioner sebagai feedback dari kegiatan ini.

Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were used to determine the bacterial species in the samples. A total of six pure isolates were identified based on morphological observation and the molecular DNA characteristics from three of them were identified with 16S rRNA gene sequences. The identification using 16S rRNA gene showed that the isolate ACD.P4.PH7.P had a close relationship with the BF strain and zb strain of Bacillus flexus (acc number MH569560.1) with a similarity of 85.44 %. While ACD.P4.PH9.P isolate has a close relationship with Bacillus sp. c234 (acc number FJ950647.1) with a similarity of 98.50 %. Additionally, ACD.P4.PH9.K isolates closely related to Bacillus sp. of strain 6RM1 (acc number MK134607.1) with a similarity of 94.78 %. This study also revealed that both microscopic identifications by morphological traits and molecular approach using 16S rRNA gene can be used in bacterial diversity assessment.

Background and aims – The present study aims to describe a new species of pennate blue diatom from the genus Haslea, H. nusantara sp. nov., collected from Semak Daun Island, the Seribu Archipelago, in Indonesian marine waters.Methods – Assessment for species identification was conducted using light microscopy, Scanning Electron Microscopy and molecular techniques. The morphological characteristics of H. nusantara have been described, illustrated and compared to other morphologically similar blue Haslea taxa, distributed worldwide. Additionally, molecular characterization was achieved by sequencing plastidial and mitochondrial genomes.Key results – This new species, named Haslea nusantara, cannot be discriminated by its morphology (stria density) but it is characterized by its gene sequences (rbcL chloroplast gene and cox1 mitochondrial gene). Moreover, it differentiates from other blue Haslea species by the presence of a thin central bar, which has been previously reported in non-blue species like H. pseudostrearia. The complete mitochondrion (36,288 basepairs, bp) and plastid (120,448 bp) genomes of H. nusantara were sequenced and the gene arrangements were compared with other diatom genomes. Phylogeny analyses established using rbcL indicated that H. nusantara is included in the blue Haslea cluster and close to a blue Haslea sp. found in Canary Islands (H. silbo sp. ined.).Conclusions – All investigations carried out in this study show that H. nusantara is a new blue-pigmented species, which belongs to the blue Haslea clade, with an exceptional geographic distribution in the Southern Hemisphere.

Milkfish is potential fish with high production and belong to ten of fish commodities culture in Indonesia. Pond stability of milkfish especially in fingerling phase (young phase) influenced by several factors including water quality and organism availability (periphyton). This recent study aimed to assess the milkfish traditional pond stability in fingerling phase based on water quality and periphyton availability aspects. These aspects are quite important to milkfish production and their growth as well as their food habit. The research was conducted in traditional pond located in UPT Fisheries Brackish and Saline Water Probolinggo for three months. Water quality and periphyton analyses were in situ and ex situ. The result shows that all the water quality is belong to good condition (stable) except in ammonia, TOM, and orthophosphate. However, this fluctuated value is not significantly impacting to the living organisms (milkfish). The research concluded that the fingerling milkfish traditional pond is belonging to moderate to stable condition.

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic

Abstract. Empowerment program for grouper fish farmers in Labuhan Village, Labuhan Subdistrict, Lamongan Regency, aimed to share knowledge, increase partner understanding and skills on semi-intensive aquaculture ponds, monitor water quality parameters through water quality measurement training, and process grouper fish aquaculture using appropriate technology by introducing automatic feeder devices independently. This community service activity has been carried out in the Bhakti Usaha II Farmer Group in Labuhan Village, Lamongan Regency, targeting grouper fish farmer partner. This activity started from Mei to November 2018. The program consists of location survey, discussion and training about water quality measurement, as well as socialization and testing of automatic feeder machine, and monitoring and evaluation at the end. The discussion material presented includes criteria for grouper fish farming, feed management, and automatic feeder use. An auto feeder application in ponds is used for feeding efficiency and so that the feed given is absorbed by fish effectively. This is expected to reduce the cost of feed and farm power efficiency and increase production of cultivation crops and partner income. Overall, the empowerment program has reached 100% of activities, which the positive feedback from grouper fish partner is determined by questionnaire analysis. Abstrak. Pemberdayaan pada kelompok pembudidaya ikan kerapu di Desa Labuhan Kecamatan Labuhan Kabupaten Lamongan bertujuan untuk berbagi pengetahuan, peningkatan pemahaman dan keterampilan mitra dalam peningkatan teknologi semi intensif di tambak budidaya ikan kerapu, pemantauan kualitas air melalui pelatihan pengukuran kualitas air, dan proses budidaya ikan kerapu melalui pemanfaatan teknologi tepat guna dengan pengenalan alat automatic feeder secara mandiri. Kegiatan pengabdian kepada masyarakat ini dilaksanakan pada Kelompok Petani Tambak Bhakti Usaha II yang berada di Desa Labuhan Kabupaten Lamongan dengan menyasar 1 mitra yang merupakan pembudidaya ikan kerapu. Kegiatan DM ini dimulai pada Bulan Mei sampai November 2018. Kegiatan yang dilaksanakan yaitu survei lokasi budidaya, penyuluhan dan pelatihan pengukuran kualitas air, sosialisasi dan uji coba mesin automatic feeder, serta monitoring dan evaluasi kegiatan. Materi penyuluhan yang disampaikan meliputi kriteria budidaya ikan kerapu, manajemen pakan, dan penggunaan automatic feeder. Aplikasi autofeeder di tambak digunakan untuk efisiensi pemberian pakan dan agar pakan yang diberikan terserap oleh ikan secara efektif. Hal ini diharapkan dapat mengurangi cost pakan dan efisiensi tenaga tambak serta meningkatkan produksi hasil panen budidaya dan pendapatan mitra. Pelaksanaan kegiatan telah mencapai 100% dan memperoleh feed back positif dari masyarakat pembudidaya melalui kuesioner yang diberikan.

Permasalahan budidaya ikan kerapu (Epinephlus sp.) di desa Labuahan adalah serangan hama dan penyakit, produksi tambak menurun terkait dengan sediaan pakan tergantung pada musim ikan rucah dan kulitas pakannya. Kegiatan IbM ini ingin memperbaiki teknik budidaya semi-intensif yang digunakan dan sediaan dan kualitas pakannya. Metode menggunakan pendekatan partisipatif. model pendekatan ditekankan pada Experience Lerning Cycle (ELC) dimana petani tambak belajar dari siklus pengalamannya sendiri. Hasil kegiatan menunjukkan bahwa teknologi budidaya yang diterapkan semi-intensif hal ini terlihat dari ukuran petak tambak relative kecil (2000 m2 – 5000 m2), padat tebar 1 ekor/m2, adanya kincir dan pakan. Selanjutnya saluran pemasukan dan pembuangan air masih menjadi satu, tidak ada pengelolaan kualitas air dan pakan yang diberikan berupa ikan rucah yang tergantung pada musim ikan. Hasil evaluasi perlu perbaikan teknik budidaya semi-intensif yang digunakan khususnya terkait: pengadaan saluran pemasukan air, pengelolaan kualitas air sebelum, saat pemeliharaan dan sesudah pemeliharaan dan perlu pengadaan pakan secara mandiri.

Abstrak Mata Air Sumberawan merupakan sumber penyedia kebutuhan air bersih bagi masyarakat Desa Toyomarto Kecamatan Singosari Kabupaten Malang yang bernaung dalam lembaga kemasyarakatan HIPPAM (Himpunan Pengguna Pemakai Air), dan juga dimanfaatkan untuk memenuhi kebutuhan masyarakat luar desa yang dikelola oleh Perusahaan Daerah Air Minum (PDAM). Dengan semakin meningkatnya kebutuhan air bersih, maka pengelolaan sumberdaya air yang tepat, sangatlah diperlukan. Beberapa hal yang dapat dilakukan dalam kaitan pengelolaan Mata Air Sumberawan adalah dengan mengetahui profil mengenai upaya pengelolaan mata air yang dilakukan oleh masyarakat di Desa Toyomarto, menganalisa tingkat kapasitas masyarakat Desa Toyomarto dalam upaya pengelolaan Mata Air Sumberawan, dan mendapatkan faktor pendorong dan penghambat dalam upaya pengelolaan mata air di Desa Toyomarto. Hasil studi menunjukkan upaya konservasi, yaitu pemanfaatan, perlindungan dan pelestarian Mata Air Sumberawan sudah dilakukan masyarakat Desa Toyomarto, namun secara keseluruhan masih didominasi oleh aspek pemanfaatan. Untuk tingkat kapasitas masyarakat Desa Toyomarto dalam pengelolaan Mata Air Sumberawan sudah cukup tinggi, ditunjukkan dari jumlah masyarakat yang mengerti terhadap manfaat peduli terhadap lingkungan yang berhubungan dengan ketersediaan air. Mengingat kepedulian dalam pengelolaan konservasi mata air telah tertanam dalam sebagian besar masyarakat Desa Toyomarto maka hal yang harus dilakukan adalah meningkatkan kegiatan konservasi ke lingkup yang lebih luas, sehingga masyarakat yang belum peduli akan sadar dan ikut terlibat dalam pengelolaan konservasi Mata Air Sumberawan. Abstract Sumberawan Wellspring is a source provider of clean water for villagers in Toyomarto Singosari Malang whose take shelter in community institutions of HIPPAM (Himpunan Pengguna Pemakai Air), and is also used to the needs of the community outside the village which is managed by the Perusahaan Daerah Air Minum (PDAM). Some things that can be done in terms of the management of Sumberawan Wellspring is to know the profile of the efforts of the management of the wellspring by the community Toyomarto Village, analyze the level of community capacity in an effort to the management of Sumberawan Wellspring, and to get powerful and inhibitors factors in an effort to manage wellsprings in the Toyomarto Village The results of a study showing effort conservation, namely aspect of the utilization, conservation and preservation in Sumberawan wellspring have been done by community of Toyomarto Village, but the whole are still dominated by utilization aspects. The capacity level of the Toyomarto village community in the management of Sumberawan Wellspring already quite high which indicated the number of people who have understood the benefits of environmental care related to availability clean water. Considering concern in the management of springs conservation has embedded in most of the community of Toyomarto Village then thing to do is improve conservation activities to scope broader, so that people who do not care to be aware of and involved in conservation management Sumberawan Wellspring

Abstrak Penelitian ini bertujuan untuk melakukan monitoring kualitas air di tambak budidaya udang vaname, membandingkan efektivitas penerapan budidaya dengan sistem pemeliharaan berbeda pada tambak dan variasi pemberian pakan. Pada kegiatan ini, empat tambak budidaya digunakan sebagai tempat pembesaran udang vaname (Litopenaeus vannamei). Parameter yang diukur meliputi parameter fisika dan kimia yaitu suhu, kecerahan, pH, oksigen terlarut, salinitas, amonia, dan alkalinitas; sedangkan performa pertumbuhan organisme budidaya dilihat dengan cara menghitung tingkat kelulushidupan (survival rate) udang pada akhir pemeliharaan, efisiensi konsumsi pakan melalui perhitungan FCR, dan laju pertumbuhan spesifik udang (SGR) dengan menghitung ABW (Average Body weight) dan ADG (Average Daily Growth) udang. Hasil penelitian menunjukan bahwa secara keseluruhan kisaran kualitas air yang diperoleh masih dalam keadaan layak untuk kegiatan budidaya dan bahkan Tambak 3 dan 4 menunjukkan kisaran optimum untuk kualitas air budidaya, sedangkan untuk parameter performa pertumbuhan, pada Tambak 3 dan 4 diperoleh nilai SR lebih dari 80 %, dan Tambak 1 dan 2 mempunyai SR di bawah 70 %. Selain itu, nilai FCR berada di bawah 1.7 pada tambak 3 dan 4, sedangkan pada Tambak 1 dan 2 nilainya lebih dari 1.7. Terakhir untuk nilai SGR, Tambak 3 dan 4 juga menunjukkan presentasi yang bagus jika dibandingkan Tambak 1 dan 2. Secara komprehensif, dapat disimpulkan bahwa penerapan sistem pemeliharaan dengan menggunakan sistem flok pada Tambak 3 dan 4 meningkatkan performa kualitas air dan hasil produksi dibandingkan pada Tambak 1 dan 2. Abstract The aim of this study was to monitor water quality in vaname culture pond and compare the application of different rearing culture system and feeding variations. Four ponds culture were used as vaname (Litopenaeus vannamei) growth place. Measured parameters include physical and chemical factors such as temperature, brightness, pH, DO, salinity, ammonia, and alkalinity, while growth shrimp performance showed by SGR, SR and FCR. The research result of the water quality parameters show an adequate range values for all of the ponds and good enough for shrimp growth, and especially an optimum range value presented in pond three and four. Survival rate (SR) both pond 3 and 4 exhibit a good presentation that is more than 80%, whereas pond 1 and 2 were just less than 70% of SR value. The specific growth rate (SGR) presents also a good presentation in Pond 3 and 4 rather than pond 1 and 2. Based on the feed consumption, pond 1 and 2 show high FCR that is more than 1.7 while pond 3 and 4 present smaller FCR value which is less than 1.7. Finally, it could be concluded that application of floc in culture rearing system of pond 3 and 4 increase water quality and production value than pond 1 and 2.

In diatoms, the main photosynthetic pigments are chlorophylls a and c, fucoxanthin, diadinoxanthin and diatoxanthin. The marine pennate diatom Haslea ostrearia has long been known for producing, in addition to these generic pigments, a water-soluble blue pigment, marennine. This pigment, responsible for the greening of oysters in western France, presents different biological activities: allelopathic, antioxidant, antibacterial, antiviral, and growth-inhibiting. A method to extract and purify marennine has been developed, but its chemical structure could hitherto not be resolved. For decades, H. ostrearia was the only organism known to produce marennine, and can be found worldwide. Our knowledge about H. ostrearia-like diatom biodiversity has recently been extended with the discovery of several new species of blue diatoms, the recently described H. karadagensis, H. silbo sp. inedit. and H. provincialis sp. inedit. These blue diatoms produce different marennine-like pigments, which belong to the same chemical family and present similar biological activities. Aside from being a potential source of natural blue pigments, H. ostrearia-like diatoms thus present a commercial potential for aquaculture, cosmetics, food and health industries.