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343 results on '"Sukhanova, Alyona"'

3. Photonic Crystal Surface Mode Imaging for Multiplexed Real-Time Detection of Antibodies, Oligonucleotides, and DNA Repair Proteins

Author

Nifontova Galina, Gerasimovich Evgeniia, Fleury Fabrice, Sukhanova Alyona, and Nabiev Igor

Subjects
Physics, QC1-999
Abstract

Sensors based on photonic crystal (PC) surface mode imaging are promising tools for label-free drug screening and discovery, diagnostics, and analysis of ligand–receptor interactions. Imaging of PC surface modes has been demonstrated to allow simultaneous real-time detection of multiple events at the sensor surface. Here, we report the engineering of a lateral-flow microfluidic assay where PC surface mode imaging is used for multiplexed detection of biomolecular targets (antibodies, oligonucleotides, and a DNA repair protein), as well as kinetic data on their interactions obtained without additional labelling or signal amplification. Our data demonstrate the suitability of the biosensing platform designed for ultrasensitive, quick, and low-cost detection and monitoring of interactions between different biomolecules.

Published
2023
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5. Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies.

Author

Sokolov, Pavel, Evsegneeva, Irina, Karaulov, Alexander, Sukhanova, Alyona, and Nabiev, Igor

Subjects
PROTEIN microarrays, DIETARY patterns, ALLERGIES, EXTRACELLULAR matrix proteins, BIOCHEMICAL substrates
Abstract

The prevalence of allergic diseases has increased tremendously in recent decades, which can be attributed to growing exposure to environmental triggers, changes in dietary habits, comorbidity, and the increased use of medications. In this context, the multiplexed diagnosis of sensitization to various allergens and the monitoring of the effectiveness of treatments for allergic diseases become particularly urgent issues. The detection of allergen-specific antibodies, in particular, sIgE and sIgG, is a modern alternative to skin tests due to the safety and efficiency of this method. The use of allergen microarrays to detect tens to hundreds of allergen-specific antibodies in less than 0.1 mL of blood serum enables the transition to a deeply personalized approach in the diagnosis of these diseases while reducing the invasiveness and increasing the informativeness of analysis. This review discusses the technological approaches underlying the development of allergen microarrays and other protein microarrays, including the methods of selection of the microarray substrates and matrices for protein molecule immobilization, the obtainment of allergens, and the use of different types of optical labels for increasing the sensitivity and specificity of the detection of allergen-specific antibodies. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Planar SERS sensors for SARS-CoV-2 virus detection

Author

Sarychev, Andrey K., primary, Ivanov, Andrey V., additional, Bykov, Igor V., additional, Bakholdin, Nikita V., additional, Mochalov, Konstantin E., additional, Shestopalova, Milena S., additional, Oleinikov, Vladimir A., additional, Gushchin, Vladimir A., additional, Nabiev, Igor R., additional, and Sukhanova, Alyona V., additional

Published
2023
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18. Photonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate.

Author

Nifontova, Galina, Charlier, Cathy, Ayadi, Nizar, Fleury, Fabrice, Karaulov, Alexander, Sukhanova, Alyona, and Nabiev, Igor

Subjects
DNA repair, PHOTONIC crystals, CRYSTAL surfaces, PROTEIN-protein interactions, HOMOLOGOUS recombination, OLIGONUCLEOTIDES
Abstract

Photonic crystals (PCs) are promising tools for label-free sensing in drug discovery screening, diagnostics, and analysis of ligand–receptor interactions. Imaging of PC surface modes has emerged as a novel approach to the detection of multiple binding events at the sensor surface. PC surface modification and decoration with recognition units yield an interface providing the highly sensitive detection of cancer biomarkers, antibodies, and oligonucleotides. The RAD51 protein plays a central role in DNA repair via the homologous recombination pathway. This recombinase is essential for the genome stability and its overexpression is often correlated with aggressive cancer. RAD51 is therefore a potential target in the therapeutic strategy for cancer. Here, we report the designing of a PC-based array sensor for real-time monitoring of oligonucleotide–RAD51 recruitment by means of surface mode imaging and validation of the concept of this approach. Our data demonstrate that the designed biosensor ensures the highly sensitive multiplexed analysis of association–dissociation events and detection of the biomarker of DNA damage using a microfluidic PC array. The obtained results highlight the potential of the developed technique for testing the functionality of candidate drugs, discovering new molecular targets and drug entities. This paves the way to further adaption and bioanalytical use of the biosensor for high-content screening to identify new DNA repair inhibitor drugs targeting the RAD51 nucleoprotein filament or to discover new molecular targets. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Label-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

Author

Sarychev, Andrey K., primary, Sukhanova, Alyona, additional, Ivanov, Andrey V., additional, Bykov, Igor V., additional, Bakholdin, Nikita V., additional, Vasina, Daria V., additional, Gushchin, Vladimir A., additional, Tkachuk, Artem P., additional, Nifontova, Galina, additional, Samokhvalov, Pavel S., additional, Karaulov, Alexander, additional, and Nabiev, Igor, additional

Published
2022
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35. Designing Functionalized Polyelectrolyte Microcapsules for Cancer Treatment

Author

Kalenichenko, Daria, Nifontova, Galina, Karaulov, Alexander, Sukhanova, Alyona, Nabiev, Igor, The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia], Sechenov First Moscow State Medical University, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
doxorubicin encapsulation, optical encoding, Chemistry, fluorescence imaging, technology, industry, and agriculture, [SDV.CAN]Life Sciences [q-bio]/Cancer, quantum dots, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, QD1-999, Article, polyelectrolyte microcapsules
Abstract

The engineering of delivery systems for drugs and contrasting labels ensuring the simultaneous imaging and treatment of malignant tumors is an important hurdle in developing new tools for cancer therapy and diagnosis. Polyelectrolyte microcapsules (MCs), formed by nanosized interpolymer complexes, represent a promising platform for the designing of multipurpose agents, functionalized with various components, including high- and low-molecular-weight substances, metal nanoparticles, and organic fluorescent dyes. Here, we have developed size-homogenous MCs with different structures (core/shell and shell types) and microbeads containing doxorubicin (DOX) as a model anticancer drug, and fluorescent semiconductor nanocrystals (quantum dots, QDs) as fluorescent nanolabels. In this study, we suggest approaches to the encapsulation of DOX at different stages of the MC synthesis and describe the optimal conditions for the optical encoding of MCs with water-soluble QDs. The results of primary characterization of the designed microcarriers, including particle analysis, the efficacy of DOX and QDs encapsulation, and the drug release kinetics are reported. The polyelectrolyte MCs developed here ensure a modified (prolonged) release of DOX, under conditions close to normal and tumor tissues, they possess a bright fluorescence that paves the way to their exploitation for the delivery of antitumor drugs and fluorescence imaging.

Published
2021
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40. Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

Author

Mochalov, Konstantin, Vaskan, Ivan, Sarychev, Andrey, Ivanov, Alexandre, Bykov, Igor, Bakholdin, Nikolai, Vasina, Daria, Gushchin, Vladimir, Tkachuk, Artem, Nifontova, Galina, Samokhvalov, Pavel, Sukhanova, Alyona, Nabiev, Igor, Nabiev, Igor, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh RAS), Russian Academy of Sciences [Moscow] (RAS), Institute of Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow Power Engineering Institute (MPEI), Gamaleya Research Center, The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia], Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy is a surface- or cavity-enhanced variant of Raman scattering spectroscopy allowing the detection of analytes with a sensitivity down to single molecules. The main prerequisite for the effectiveness of this method is the development of SERS-active surfaces or cavities capable of concentrating incident radiation into small mode volumes containing the analyte. Here, we have demonstrated that concentration of light in an ultranarrow metal–dielectric nano-cavity between a film of the receptor-binding domain (RBD) of SARS-CoV-2 S glycoprotein and silver surface, which is formed via interaction of reduced sulfhydryl groups of the RBD with silver, allows recording SERS spectra at concentrations sufficient for ultrasensitive detection of viral protein antigens at physiologically relevant (sub-picogram) levels. Moreover, an additional increase in the cavity Q-factor upon coating the dielectric RBD film with a nanometer-thick silver shell results in an additional order(s) of magnitude enhancement of the protein SERS signal, thus ensuring a sub-femtogram sensitivity of the viral antigen detection. A simplified theoretical model explaining the observed additional enhancement of the SERS signal from the protein coated with a silver film is proposed.Our study is the first to obtain characteristic Raman and SERS spectra of the RBD of S glycoprotein, the key SARS-CoV-2 viral antigen directly, without the use of low-molecular-weight Raman-reporter molecules. The possibility of direct recording of characteristic spectra of viral protein antigens at the concentration orders of magnitude lower than those required for the detection of the whole virus in biological media makes the development of a high-performance optical method for detecting and conformational analyzing the pathogen variants a realistic task.

Published
2021

41. Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications

Author

Polímeros y Materiales Avanzados: Física, Química y Tecnología, Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia, Nifontova, Galina, Krivenkov, Victor, Zvaigzne, Mariya, Efimov, Anton, Korostylev, Evgeny, Zarubin, Sergei, Karaulov, Alexander, Nabiev, Igor, Sukhanova, Alyona, Polímeros y Materiales Avanzados: Física, Química y Tecnología, Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia, Nifontova, Galina, Krivenkov, Victor, Zvaigzne, Mariya, Efimov, Anton, Korostylev, Evgeny, Zarubin, Sergei, Karaulov, Alexander, Nabiev, Igor, and Sukhanova, Alyona

Abstract

Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging.

Published
2021

42. Nanoparticle-doped hybrid polyelectrolyte microcapsules with controlled photoluminescence for potential bioimaging applications

Author

Russian Science Foundation, Agence Nationale de la Recherche (France), European Commission, Nifontova, Galina, Krivenkov, Victor, Zvaigzne, Mariya, Efimov, Anton, Korostylev, Evgeny, Zarubin, Sergei, Karaulov, Alexander, Nabiev, Igor, Sukhanova, Alyona, Russian Science Foundation, Agence Nationale de la Recherche (France), European Commission, Nifontova, Galina, Krivenkov, Victor, Zvaigzne, Mariya, Efimov, Anton, Korostylev, Evgeny, Zarubin, Sergei, Karaulov, Alexander, Nabiev, Igor, and Sukhanova, Alyona

Abstract

Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging.

Published
2021

45. Graphene quantum dots unraveling: Green synthesis, characterization, radiolabeling with 99mTc, in vivo behavior and mutagenicity

Author

de Menezes, Frederico Duarte, dos Reis, Sara Rhaissa Rezende, Pinto, Suyene Rocha, Portilho, Filipe Leal, do Vale Chaves e Mello, Francisco, Helal-Neto, Edward, da Silva de Barros, Aline Oliveira, Alencar, Luciana Magalhães Rebêlo, de Menezes, Alan Silva, dos Santos, Clenilton Costa, Saraiva-Souza, Aldilene, Perini, Jamila Alessandra, Machado, Daniel Escorsim, Felzenswalb, Israel, Araujo-Lima, Carlos Fernando, Sukhanova, Alyona, Nabiev, Igor, and Santos-Oliveira, Ralph

Published
2019
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46. Controlling Charge Transfer from Quantum Dots to Polyelectrolyte Layers Extends Prospective Applications of Magneto-Optical Microcapsules

Author

Nifontova, Galina, primary, Krivenkov, Victor, additional, Zvaigzne, Maria, additional, Samokhvalov, Pavel, additional, Efimov, Anton E., additional, Agapova, Olga I., additional, Agapov, Igor I., additional, Korostylev, Evgeny, additional, Zarubin, Sergei, additional, Karaulov, Alexander, additional, Nabiev, Igor, additional, and Sukhanova, Alyona, additional

Published
2020
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47. Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells

Author

Nifontova, Galina, Kalenichenko, Daria, Baryshnikova, Maria, Ramos Gomes, Fernanda, Alves, Frauke, Karaulov, Alexander, Nabiev, Igor, Sukhanova, Alyona, The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia], Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), N.N. Blokhin National Medical Research Center of Oncology, Max Planck Institute of Experimental Medicine [Göttingen] (MPI), Max-Planck-Gesellschaft, University Medical Center Göttingen (UMG), and Sechenov First Moscow State Medical University

Subjects
lcsh:Applied optics. Photonics, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, lcsh:TA1501-1820, quantum dots, fluorescent nanolabels, [CHIM.MATE]Chemical Sciences/Material chemistry, fluorescent imaging, polyelectrolyte microcapsules, antibody-mediated targeted delivery
Abstract

International audience; Fluorescent semiconductor nanocrystals or quantum dots (QDs) are characterized by unique optical properties, including a high photostability, wide absorption spectrum, and narrow, symmetric fluorescence spectrum. This makes them attractive fluorescent nanolabels for the optical encoding of microcarriers intended for targeted drug delivery, diagnosis, and imaging of transport processes on the body, cellular, and subcellular levels. Incorporation of QDs into carriers in the form of polyelectrolyte microcapsules through layer-by-layer adsorption of oppositely charged polyelectrolyte polymers yields microcapsules with a bright fluorescence signal and adaptable size, structure, and surface characteristics without using organic solvents. The easily modifiable surface of the microcapsules allows for its subsequent functionalization with capture molecules, such as antibodies, which ensures specific and selective interaction with cells, including tumor cells, with the use of the bioconjugation technique developed here. We obtained stable water-soluble nanolabels based on QDs whose surface was modified with polyethylene glycol (PEG) derivatives and determined their colloidal and optical characteristics. The obtained nanocrystals were used to encode polyelectrolyte microcapsules optically. The microcapsule surface was modified with humanized monoclonal antibodies (Abs) recognizing a cancer marker, epidermal growth factor receptor (EGFR). The possibility of effective, specific, and selective delivery of the microcapsules to tumor cells expressing EGFR has been demonstrated. The results show that the QD-encoded polyelectrolyte microcapsules functionalized with monoclonal Abs against EGFR can be used for targeted imaging and diagnosis.

Published
2019
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48. Raman and CD spectroscopy of recombinant 68-kDa DNA human topoisomerase I and its complex with suicide DNA-substrate

Author

Fleury, Fabrice, Ianoul, Anatoli, Kryukov, Eugene, Sukhanova, Alyona, Kudelina, Irina, Wynne-Jones, Abigail, Bronstein, Igor B., Maizieres, Michael, Berjot, Maurice, Dosson, Guy G., Wilkinson, Anthony J., Holden, Joseph A., Feofanov, Alexei, alix, Alain J.P., Jardillier, Jean-Claude, and Nabiev, Igor

Subjects
DNA topoisomerase I -- Research, Eukaryotic cells -- Observations, Biological sciences, Chemistry
Abstract

The eukaryotic topoisomerase (topo) I-induced DNA relaxation reaction involves DNA recognition, binding cleavage, swiveling, religation and re-adoption of an initial enzyme conformation. A comparative structural characterization of recombinant human 68-kDa topo I from lysine-191 has been undertaken. Suicide sequence-specific oligonucleotide was used n the uncoupling of 68-kDa topo I cleavage. There was evidence of significant conformational transition during 68-kDa human topo I/DNA binding and cleavage reactions.

Published
1998

49. MPSA abstracts

Author

Aminlari, Mahmoud, Asquith, Thomas, Sarlo, Katherine, Bailey, Jerome M., Tu, Oanh, Issai, Gilbert, Ha, Alice, Shively, John E., Bell, Alexander W., Baur, Nicole C., Bergeron, John J. M., Ou, Wei -Jia, Thomas, David Y., Cianflone, Katherine, Baldo, Allain, Hincke, Maxwell T., Momparler, Richard L., Laliberté, Josée, Thomson, David M. P., Sutherland, M., Besada, Vladimir, Gonzalez, Javier, Padron, Gabriel, Garay, Hilda, Reyes, Osvaldo, Takao, Toshifumi, Shimonishi, Yasutsugu, Bischoff, Rainer, Roecklin, Dominique, Bouchon, Bernadette, Klarskov, Klaus, Van Dorsselaer, Alain, Brake, Patricia G., Pacitti, Anne, Higgins, Terry, Stevis, Panos, Malinowski, John, McElhiney, Sue, Huang, Janes, Vestal, Christine, Buckel, Scott D., Stevenson, Tracy, Loo, Joseph A., Caffrey, Martin, Wang, Jin, Wallace, Carmichael J. A., Clark-Lewis, Ian, Carraway, C. A. Carothers, Huang, J., Li, Y., Juang, S. -H., Gallo, A., Mayer, B. J., Carraway, K. L., Coleman, Patrick L., Sarpong, Daniel, Deerfield, II, David W., Holland-Minkley, Amanda, Hempel, John D., Nicholas, Jr., Hugh B., Denslow, Nancy D., Folmar, Leroy C., Sullivan, Craig V., Dixon, James D., Mark, Jonathan P., Elicone, Christopher P., Maleknia, Simin D., McGuinness, Brian F., Regnier, Fred E., Afeyan, Noubar B., Dolence, Julia M., Poulter, C. Dale, Egorov, Tsezi, Musolyamov, Alexander, Popineau, Yves, Andersen, Jens, Roepstorff, Peter, Falkenstein, Roberto J., Biscoglio de Jiménez Bonino, Mirtha J., Peña, Clara, Gauggel, D. L., Asquith, T. N., Isfort, R. J., Miller, N. S., Cody, D. B., Giblin, Michael F., Wong, Tuck C., Quinn, Thomas P., Grant, Gregory A., Crankshaw, Mark W., Griffith, Scott, Schroeder, Steve, Quinn, Thomas, Guinet, F., Petillot, Y., Chapsal, J. M., Dubayle, J., Greco, F., Barge, O., Forest, E., Valentin, C., Hahn, Frederick M, Baker, Jonathan A., Poulter, C. Dale, Haniu, Mitsuru, Kenney, William C., Rohde, Michael F., Harman, James G., Lee, Eun Ju, Glasgow, Joel, Lew, Sew Fen, Belduz, Ali O., Harris, Reed J., Molony, Michael S., Keyt, Lene H., Wu, Shiaw -Lin, Hawke, David H., Tso, Jaqueline, Early, Sherrell, Miller, Chad G., Hayman, G. Thomas, Miernyk, Jan A., Hellman, Ulf, Wernstedt, Christer, Góñez, Jorge, Hess, Daniel, Studer, Ralph, Hunziker, Peter E., Hirano, Hisashi, Watanabe, Yoshihiro, Barbashov, Sergei F., Komatsu, Setsuko, Hemmings, Andrew M., Miyagi, Masaru, Tsunasawa, Susumu, Huber, Reuben E., Roth, Nathan J., Gaunt, Michael T., Jenö, Paul, Mini, Thierry, Moes, Suzette, Horst, Martin, Jinnai, Kenji, Ashizawa, Tetsuo, Atassi, M. Zouhair, Johnsen, Anders H., Jensen, Hanne, Rehfeld, Jens F., Kamo, Masaharu, Kawakami, Takao, Miyatake, Norifumi, Tsugita, Akira, Keen, JN, Zagalsky, PF, Findlay, JBC, Kraft, Regine, Kostka, Susanne, Hartmann, Enno, Krutzsch, Henry C., Inman, John K., Machalinski, Claudia, Bonino, Mirtha Biscoglio de Jiménez, McRorie, Donald K., Dieckmann, Gregg R., Heilman, Susan, DeGrado, William F., Pecoraro, Vincent L., Kenny, James, Sahakian, Julie, Tso, Jacqueline, Moyer, Mary B., Burkhart, William A., Muranova, Tatyana, Makova, Lubov, Nicholas, Hugh, Hempel, John, Hinich, Amy, Deerfield, David, Behrmann, Joseph, Ropelewski, Alex, Nixon, Lori, Maneri, Leonard, Nugent, Kerry, Stoney, Ken, Wieser, John, Ohguro, Hiroshi, Palczewski, Krzysztof, Walsh, Kenneth A., Johnson, Richard S., Packman, Leonard C, Webster, Carl, Gray, John, Padrón, G., Morera, V., González, L. J., Támbara, Y., Besada, V., Villalonga, R., Chinea, G., Reyes, O., Bringas, H. Garay R., Nazábal, C., Parkinson, Bruce P., Yamada, Kent A., Randolph, Anne, Pisano, Anthony, Packer, Nicole H., Redmond, John W., Williams, Keith L., Gooley, Andrew A., Rasmussen, Hanne H., Mørtz, Ejvind, Mann, Matthias, Celis, Julio E., Rasmussen, Lone K., Sørensen, Esben S., Petersen, Torben E., Gliemann, Jørgen, Jensen, Poul Henning, Renlund, Staffan, Wadensten, Henrik, Persson, Annika, Persson, Per, Johansson, Agneta, Edlund, Per -Olof, Rose, Donald J., Sack, Ragna, Apffel, Alex, Miller, Chad, Levine, Rodney L., Sakaguchi, Kazuyasu, Zambrano, Nicola, Lewis, Marc S., Baldwin, Eric T., Shapiro, Bruce A., Erickson, John W, Omichinski, James G., Clore, G. Marius, Gronenborn, Angela M., Appella, Ettore, Schröder, Werner, Moser, Irmgard, Pansegrau, Werner, Lanka, Erich, Simpson, Richard J., Eddes, James, Ji, Hong, Reid, Gavin E., Moritz, Robert L., Højrup, Peter, Speicher, David W., Reim, David F., Speicher, Kaye D., Srinivasa, B. R., Barde, S. P., Stirtan, William G., Sukhanova, Alyona, Vorob'ev, Sergey, Gabibov, Alexander, Bronstein, Igor, Tanaka, Kenji, Einaga, Kuniko, Tsukada, Minoru, Tait, Jonathan F., Fujikawa, Kazuo, Takamoto, Keiji, Satake, Kazuo, Vakser, Ilya A., Velikodvorskaia, V. V., Gabibov, A. G., Rabinkov, A. G., Videler, Tennie, Osborne, Michael, Moore, Geoffrey, James, Richard, Kleanthous, Colin, Walent, Jane H., Bessen, Richard, Marsh, Dick, Clore, G. Marius, Niece, Ronald L., Tsao, Francis H. C., Wang, Hong, Weinberger, Scot R., Chakel, Lynn M., Wondrak, Ewald M., Kimmel, Alan R., and Louis, John M.

Published
1994
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