Cichorium intybus or chicory was used in traditional medicine for curing several diseases such as diarrhea, to improve the prostate and other reproductive organs, to treat lung and tobacco disease, cancer, severe headache and others. This study aims to assess the C. intybus extracts (leaf, stem and root) for their antioxidant efficacy and cytotoxicity. Total phenol content was estimated in C. intybus extracts (leaf, stem, and root) by Folin-Ciocalteu’s assay. The phenol content value of the leaf extract was (124.74±0.6 mg GAE), stem (65.52±0.96 mg GAE), and root extract (56.76 ± 0.5mg/g GAE). In vitro, the antioxidant assess were done using DPPH and ferric reducing power assay, all extract appeared a significant antioxidant capacity rate in a dose-dependent manner, IC50 values of Ascorbic acid and leaf, stem and root extract of C. intybus were found to be 12.15, 33.92, 31.7 and 65.79 μg/ml, respectively. Cytotoxicity of C. intybus extracts was done on two types of cell lines, CAL-51 cell line and normal Vero cell line, high significant inhibition value was detected in the CAL-51 and moderate or less effect of normal Vero cell line at three periods of exposure (24, 48 and 72 hours). [ABSTRACT FROM AUTHOR]