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26. Functional Characterization of the CpNAC1 Promoter and Gene from Chimonanthus praecox in Arabidopsis.

Author

Zhao, Xiaoyan, Zhao, Jiahui, Yang, Qing, Huang, Min, Song, Yangjing, Li, Mingyang, Sui, Shunzhao, and Liu, Daofeng

Subjects
DROUGHT tolerance, ARABIDOPSIS, GENE families, ABIOTIC stress, TRANSCRIPTION factors, PLANT growth
Abstract

The NAC (NAM, ATAF, and CUC) gene family is one of the largest plant-specific transcription factor families. Its members have various biological functions that play important roles in regulating plant growth and development and in responding to biotic and abiotic stresses. However, their functions in woody plants are not fully understood. In this study, we isolated an NAC family member, the CpNAC1 promoter and gene, from wintersweet. CpNAC1 was localized to the nucleus and showed transcriptional activation activity. qRT-PCR analyses revealed that the gene was expressed in almost all tissues tested, with the highest levels found in mature leaves and flower buds. Moreover, its expression was induced by various abiotic stresses and ABA treatment. Its expression patterns were further confirmed in CpNAC1pro:GUS (β-glucuronidase) plants. Among all the transgenic lines, CpNAC1pro-D2 showed high GUS histochemical staining and activity in different tissues of Arabidopsis. Furthermore, its GUS activity significantly increased in response to various abiotic stresses and ABA treatment. This may be related to the stress-related cis-elements, such as ABRE and MYB, which clustered in the CpNAC1pro-D2 segment, suggesting that CpNAC1pro-D2 is the core segment that responds to abiotic stresses and ABA. In addition, CpNAC1-overexpressed Arabidopsis plants had weaker osmosis tolerance than the wild-type plants, demonstrating that CpNAC1 may negatively regulate the drought stress response in transgenic Arabidopsis. Our results provide a foundation for further analyses of NAC family genes in wintersweet, and they broaden our knowledge of the roles that NAC family genes may play in woody plants. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Full-Length Transcriptome Characterization and Functional Analysis of Pathogenesis-Related Proteins in Lilium Oriental Hybrid 'Sorbonne' Infected with Botrytis elliptica.

Author

Du, Wenting, Chai, Nan, Sun, Zhengqiong, Wang, Huiru, Liu, Sixian, Sui, Shunzhao, Luo, Lan, and Liu, Daofeng

Subjects
LILIES, FUNCTIONAL analysis, MICROSATELLITE repeats, PROTEIN analysis, TRANSCRIPTOMES, BOTRYTIS, JASMONATE
Abstract

Gray mold (Botrytis elliptica) causes a deleterious fungal disease that decreases the ornamental value and yield of lilies. Lilium oriental hybrid 'Sorbonne' is a variety that is resistant to gray mold. Understanding the mechanism of resistance against B. elliptica infection in 'Sorbonne' can provide a basis for the genetic improvement in lily plants. In this study, a PacBio Sequel II system was used to sequence the full-length transcriptome of Lilium 'Sorbonne' after inoculation with B. elliptica. A total of 46.64 Gb subreads and 19,102 isoforms with an average length of 1598 bp were obtained. A prediction analysis revealed 263 lncRNAs, and 805 transcription factors, 4478 simple sequence repeats, and 17,752 coding sequences were identified. Pathogenesis-related proteins (PR), which may play important roles in resistance against B. elliptica infection, were identified based on the full-length transcriptome data and previously obtained second-generation transcriptome data. Nine non-redundant potential LhSorPR proteins were identified and assigned to two groups that were composed of two LhSorPR4 and seven LhSorPR10 proteins based on their genetic relatedness. The real-time quantitative reverse transcription PCR (qRT-PCR) results showed that the patterns of expression of nine differentially expressed PR genes under B. elliptica stress were basically consistent with the results of transcriptome sequencing. The pattern of expression of LhSorPR4s and LhSorPR10s genes in different tissues was analyzed, and the expression of each gene varied. Furthermore, we verified the function of LhSorPR4-2 gene in Lilium. The expression of LhSorPR4-2 was induced by phytohormones such as methyl jasmonate, salicylic acid, and ethephon. Moreover, the promoter region of LhSorPR4-2 was characterized by several functional domains associated with phytohormones and stress response. The overexpression of LhSorPR4-2 gene in 'Sorbonne' increased the resistance of the lily plant to B. elliptica and correlated with high chitinase activity. This study provides a full-length transcript database and functionally analyzed the resistance of PR gene to B. elliptica in Lilium, thereby introducing the candidate gene LhSorPR4-2 to breed resistance in Lilium. [ABSTRACT FROM AUTHOR]

Published
2023
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33. The Experiments of Grafting Propagation for Wintersweet (Chmonanthus praecox) in Autumn and Winter

Author

Ting Zhu, Ximeng Yang, Guixiang Li, Huan Hu, Xia Wang, Sui Shunzhao, Mingyang Li, and Li Tao

Subjects
Horticulture, Perennial plant, media_common.quotation_subject, Wintersweet, Reproduction, Biology, Rootstock, Grafting, media_common
Abstract

Taking different varieties of wintersweet ( Chmonanthus praecox ) as test materials, the influence of different grafting times, stock ages, and scion positions on the survival percentage of wintersweet grafting were studied, in order to study the key techniques of wintersweet grafting in autumn and winter. The results showed that, in autumn and winter, the grafting survival percentage of wintersweet in late-september was significantly higher than that of mid-october, late-october, and mid-november；with the passage of time, the survival percentage of wintersweet grafting showed a gradual downward trend；there is no obvious regularity of the effect of the different scion positions on the survival percentage of wintersweet grafting in autumn and winter；the survival percentage of grafting with perennial seedlings as rootstocks (56.03%) was significantly higher than that of annual seedlings (21.14%). This research provides technical guidance for the production, reproduction, introduction and expanding of wintersweet in autumn and winter.

Published
2021

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