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Abstract The present study investigated the effect of infrared irradiation in the immune response of BALB/c mice against infection with secondary hydatid disease. By exposing protoscoleces of Echinococcus granulosus to infrared radiation at the periods 1, 2, 3, and 5 minutes, 20 cm from the light source, at wave length of 700-750 nm. Irradiated protoscoleces were injected in mice intraperitoneally. Many criteria were taken into consideration, 3, 15, and 30 days post injection, including WBCs total and differential count, innate immunity represented by phagocytosis, in comparison with the control group (mice infected with non irradiated protoscoleces). The study revealed a significant increase (P≤0.001) in the WBCs total count in mice injected with irradiated protoscoleces, in comparison with the control group. Regarding to the WBCs differential count, a significant increase (P≤0.05) in the lymphocytes 3 days post injection was noticed, followed by increase 15 days post injection, which was persisted significantly (P≤0.05) 30 days post injection. A significant increase (P≤0.05) in neutrophils and monocytes was noticed in treated mice at all irradiation periods. A significant increase (P≤0.001) in the mean of phagocytosis in treated mice was reported, at all irradiation periods. It will be concluded that irradiation may be used as an immunostimulator against injection with secondary hydatid disease in mice.

ABSTRACT The study investigated the effect of infra red ray on the growth and development of secondary hydatid cysts of Echinococcus granulosus in mice which based on changes in cysts numbers, weights, diameters and percentage of their reduction, the changes in liver and spleen weights and their organ index. The study showed significant reduction, (p≤0.05), in secondary hydatid cysts weights and diameters in cyst which developed from irradiated protoscoleces. Also, there was a reduction in liver weights and organ index, with increasing spleen weights and organ index. The study elucidated that the best effect of infra red ray on cysts numbers, diameters, percentage of their reduction, liver weights and organ index were at 3 minutes of irradiation. Whereas, the optimum result of cysts weights was obtained at 5 minutes of radiation.