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Abstract Background The aim of this study was to determine whether and how Zn proteinate with moderate chelation strength (Zn-Prot M) can alleviate heat stress (HS)-induced intestinal barrier function damage of broilers. A completely randomized design was used for comparatively testing the effects of Zn proteinate on HS and non-HS broilers. Under high temperature (HT), a 1 (Control, HT-CON) + 2 (Zn source) × 2 (added Zn level) factorial arrangement of treatments was used. The 2 added Zn sources were Zn-Prot M and Zn sulfate (ZnS), and the 2 added Zn levels were 30 and 60 mg/kg. Under normal temperature (NT), a CON group (NT-CON) and pair-fed group (NT-PF) were included. Results The results showed that HS significantly reduced mRNA and protein expression levels of claudin-1, occludin, junctional adhesion molecule-A (JAMA), zonula occludens-1 (ZO-1) and zinc finger protein A20 (A20) in the jejunum, and HS also remarkably increased serum fluorescein isothiocyanate dextran (FITC-D), endotoxin and interleukin (IL)-1β contents, serum diamine oxidase (DAO) and matrix metalloproteinase (MMP)-2 activities, nuclear factor kappa-B (NF-κB) p65 mRNA expression level, and protein expression levels of NF-κB p65 and MMP-2 in the jejunum. However, dietary supplementation with Zn, especially organic Zn as Zn-Prot M at 60 mg/kg, significantly decreased serum FITC-D, endotoxin and IL-1β contents, serum DAO and MMP-2 activities, NF-κB p65 mRNA expression level, and protein expression levels of NF-κB p65 and MMP-2 in the jejunum of HS broilers, and notably promoted mRNA and protein expression levels of claudin-1, ZO-1 and A20. Conclusions Our results suggest that dietary Zn, especially 60 mg Zn/kg as Zn-Prot M, can alleviate HS-induced intestinal barrier function damage by promoting the expression of TJ proteins possibly via induction of A20-mediated suppression of the NF-κB p65/MMP-2 pathway in the jejunum of HS broilers.

Abstract Endometrial cancer (UCEC) is one of three major malignant tumors in women. The HOX gene regulates tumor development. However, the potential roles of HOX in the expression mechanism of multiple cell types and in the development and progression of tumor microenvironment (TME) cell infiltration in UCEC remain unknown. In this study, we utilized both the The Cancer Genome Atlas (TCGA) database and International Cancer Genome Consortium (ICGC) database to analyze transcriptome data of 529 patients with UCEC based on 39 HOX genes, combing clinical information, we discovered HOX gene were a pivotal factor in the development and progression of UCEC and in the formation of TME diversity and complexity. Here, a new scoring system was developed to quantify individual HOX patterns in UCEC. Our study found that patients in the low HOX score group had abundant anti-tumor immune cell infiltration, good tumor differentiation, and better prognoses. In contrast, a high HOX score was associated with blockade of immune checkpoints, which enhances the response to immunotherapy. The Real-Time quantitative PCR (RT-qPCR) and Immunohistochemistry (IHC) exhibited a higher expression of the HOX gene in the tumor patients. We revealed that the significant upregulation of the HOX gene in the epithelial cells can activate signaling pathway associated with tumour invasion and metastasis through single-cell RNA sequencing (scRNA-seq), such as nucleotide metabolic proce and so on. Finally, a risk prognostic model established by the positive relationship between HOX scores and cancer-associated fibroblasts (CAFs) can predict the prognosis of individual patients by scRNA-seq and transcriptome data sets. In sum, HOX gene may serve as a potential biomarker for the diagnosis and prediction of UCEC and to develop more effective therapeutic strategies.

Rice bran from 3 rice varieties, namely Daohuaxiang, Changlixiang and Ganwanxian No.40 were used as raw materials to investigate the effect of steam explosion on structural and properties of soluble dietary fiber in rice bran of different varieties. The structure alteration of SDF in rice bran treated by steam explosion was observed by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The impact of steam explosion on SDF functional properties of rice bran was studied by a series of evaluation indicators, such as water holding, oil holding, cholesterol adsorption, glucose adsorption, α-amylase inhibition and antioxidant capacity. The results showed that steam explosion could increase some absorption peaks intensity in the infrared spectrum of SDF, decrease crystallinity and form loose and porous structure of rice bran SDF from 3 rice varieties. The SDF contents in Daohuaxiang, Changlixiang and Ganwanxian No.40 rice bran by steam explosion were increased by 87.22%, 94.72% and 70.31%, respectively. Notably, the functional properties of steam-exploded rice bran from 3 rice varieties, such as water retention, oil retention, cholesterol adsorption capacity under human gastric fluid environment, glucose adsorption capacity, ABTS+ and OH free radical scavenging rate and total antioxidant capacity were significantly increased compared with control group (P

Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders related to adrenal steroid biosynthesis, and mainly caused by mutations in the CYP21A2 gene encoding 21-hydroxylase. Adrenal tumors are common in CAH, but functional adrenal tumors are rare. Here, we report a 17-year-old female with virilized external genitalia and primary amenorrhea, accompanied by a right adrenal tumor. Her 17-OHP level was normal, cortisol and androgen levels were significantly elevated, and the tumor pathology showed adrenal cortical adenoma. Gene testing for CYP21A2 showed c.518T > A in exon 4 and c.29313C > G in intron 2. The possibility of untreated classic CAH with 21-OH deficiency causing functional adrenal cortical adenoma should be considered. When clinical diagnosis highly considers CAH and cannot rule out the influence of functional adrenal tumors’ secretion function on 17-OHP, gene mutation analysis should be performed.

ObjectiveTo explore the molecular mechanism of Astragaloside IV (AS-IV) in alleviating renal fibrosis by inhibiting Urotensin II-induced pyroptosis and epithelial-mesenchymal transition of renal tubular epithelial cells.MethodsForty SD rats were randomly divided into control group without operation: gavage with 5ml/kg/d water for injection and UUO model group: gavage with 5ml/kg/d water for injection; UUO+ AS-IV group (gavage with AS-IV 20mg/kg/d; and UUO+ losartan potassium group (gavage with losartan potassium 10.3mg/kg/d, with 10 rats in each group. After 2 weeks, Kidney pathology, serum Urotensin II, and cAMP concentration were detected, and the expressions of NLRP3, GSDMD-N, Caspase-1, and IL-1β were detected by immunohistochemistry. Rat renal tubular epithelial cells were cultured in vitro, and different concentrations of Urotensin II were used to intervene for 24h and 48h. Cell proliferation activity was detected using the CCK8 assay. Suitable concentrations of Urotensin II and intervention time were selected, and Urotensin II receptor antagonist (SB-611812), inhibitor of PKA(H-89), and AS-IV (15ug/ml) were simultaneously administered. After 24 hours, cells and cell supernatants from each group were collected. The cAMP concentration was detected using the ELISA kit, and the expression of PKA, α-SMA, FN, IL-1β, NLRP3, GSDMD-N, and Caspase-1 was detected using cell immunofluorescence, Western blotting, and RT-PCR.ResultsRenal tissue of UUO rats showed renal interstitial infiltration, tubule dilation and atrophy, renal interstitial collagen fiber hyperplasia, and serum Urotensin II and cAMP concentrations were significantly higher than those in the sham operation group (p ConclusionAS-IV can alleviate renal fibrosis by inhibiting Urotensin II-induced pyroptosis of renal tubular epithelial cells by regulating the cAMP/PKA signaling pathway.

ABSTRACT: The dietary needs of calcium (Ca) and phosphorus (P) are interdependent, thus accurate evaluation of Ca and P requirements of broilers to support skeleton health and optimal growth is critical. The present study was carried out to investigate the effects of dietary Ca and nonphytate P (NPP) levels and their interactions on growth performance, tibiotarsus characteristics, tibiotarsus metabolism-related enzyme and proteins, and their gene expression of broilers, so as to provide a rational recommendation for Ca and NPP levels in diet. A total of 540 one-day-old Arbor Acres male broilers were randomly allotted to 1 of 15 treatments with 6 replicate cages of 6 birds per cage for each treatment in a completely randomized design involving a 5 × 3 factorial arrangement of treatments (5 levels of Ca × 3 levels of NPP). The birds were fed the corn–soybean meal diet containing 0.60%, 0.70%, 0.80%, 0.90%, or 1.00% Ca and 0.35%, 0.40%, or 0.45% NPP for 21 d. Dietary Ca level affected (P < 0.03) the bone mineral density, bone mineral content (BMC), breaking strength, ash percentage and ash Ca contents in tibia, which showed linear (P < 0.006) responses to dietary Ca levels. Dietary NPP level affected (P < 0.05) tibia BMC, ash percentage, and FGF23 mRNA level. Broilers that received 0.40% and 0.45% NPP had higher (P < 0.04) tibia BMC and ash percentage than those that received 0.35% NPP, but no differences (P > 0.05) were found between 0.40% and 0.45% NPP. Broilers that received 0.40% NPP had higher (P = 0.02) tibia FGF23 mRNA level than those that received 0.35% NPP, but no differences (P > 0.05) were detected between 0.40% and 0.45% NPP or 0.45% and 0.35% NPP. The interactions between dietary Ca and NPP affected (P < 0.05) ADG, ALP activity, bone gal protein, FGF23 contents, and the mRNA expression levels ALP and bone gal protein in tibia of broilers. Results from the present study indicate that dietary Ca and NPP interaction influences growth, tibiotarsus development, and related gene expression of broiler chickens. Considering all the criteria, the dietary levels of 0.90% Ca and 0.45% NPP would be optimal for both growth and tibiotarsus development of broilers fed a conventional corn–soybean meal diet from 1 to 21 d of age.

We investigated the optimum co-culture ratio with the highest biological nitrogen removal rate, revealing that chemical oxygen demand, total nitrogen (TN), and ammoniacal nitrogen (NH3-N) removal was increased in the Chlorella pyrenoidosa and Yarrowia lipolytica co-culture system at a 3:1 ratio. Compared with the control, TN and NH3-N content in the co-incubated system was decreased within 2–6 days. We investigated mRNA/microRNA (miRNA) expression in the C. pyrenoidosa and Y. lipolytica co-culture after 3 and 5 days, identifying 9885 and 3976 differentially expressed genes (DEGs), respectively. Sixty-five DEGs were associated with Y. lipolytica nitrogen, amino acid, photosynthetic, and carbon metabolism after 3 days. Eleven differentially expressed miRNAs were discovered after 3 days, of which two were differentially expressed and their target mRNA expressions negatively correlated with each other. One of these miRNAs regulates gene expression of cysteine dioxygenase, hypothetical protein, and histone-lysine N-methyltransferase SETD1, thereby reducing amino acid metabolic capacity; the other miRNA may promote upregulation of genes encoding the ATP-binding cassette, subfamily C (CFTR/MRP), member 10 (ABCC10), thereby promoting nitrogen and carbon transport in C. pyrenoidosa. These miRNAs may further contribute to the activation of target mRNAs. miRNA/mRNA expression profiles confirmed the synergistic effects of a co-culture system on pollutant disposal.

Cancer immunotherapy can effectively inhibit cancer progression by activating the autoimmune system, with low toxicity and high effectiveness. Some of cancer immunotherapy had positive effects on clinical cancer treatment. However, cancer immunotherapy is still restricted by cancer heterogeneity, immune cell disability, tumor immunosuppressive microenvironment and systemic immune toxicity. Cell membrane-coated nanoparticles (CMCNs) inherit abundant source cell-relevant functions, including “self” markers, cross-talking with the immune system, biological targeting, and homing to specific regions. These enable them to possess preferred characteristics, including better biological compatibility, weak immunogenicity, immune escaping, a prolonged circulation, and tumor targeting. Therefore, they are applied to precisely deliver drugs and promote the effect of cancer immunotherapy. In the review, we summarize the latest researches of biomimetic CMCNs for cancer immunotherapy, outline the existing specific cancer immune therapies, explore the unique functions and molecular mechanisms of various cell membrane-coated nanoparticles, and analyze the challenges which CMCNs face in clinical translation.

No information is available regarding the utilization of iron (Fe) from different Fe sources at a target tissue level. To detect differences in Fe metabolic utilization among Fe sources, the effect of intravenously injected Fe on growth performance, hematological indices, tissue Fe concentrations and Fe-containing enzyme activities and gene expressions of Fe-containing enzymes or protein in broilers was investigated. On d 22 post-hatching, a total of 432 male chickens were randomly allotted to 1 of 9 treatments in a completely randomized design. Chickens were injected with either a 0.9% (wt/vol) NaCl solution (control) or a 0.9% NaCl solution supplemented with Fe sulphate or 1 of 3 organic Fe sources. The 3 organic Fe sources were Fe chelates with weak (Fe-MetW), moderate (Fe-ProtM) or extremely strong (Fe-ProtES) chelation strength. The 2 Fe dosages were calculated according to the Fe absorbabilities of 10% and 20% every 2 d for a duration of 20 d. Iron injection did not affect (P > 0.05) ADFI, ADG or FCR during either 1 to 10 d or 11 to 20 d after injections. Hematocrit and Fe concentrations in the liver and kidney on d 10 after Fe injections, and Fe concentrations in the liver or pancreas and ferritin heavy-chain (FTH1) protein expression level in the liver or spleen on d 20 after Fe injections increased (P ≤ 0.05) as injected Fe dosages increased. When the injected Fe level was high at 20% Fe absorbability, the chickens injected with Fe-ProtES had lower (P

The current NRC dietary selenium (Se) requirement (0.15 mg/kg) of broilers from 22 to 42 d of age is primarily based on a previous study reported in 1986, which might not be applicable to modern classes of rapidly growing broilers. The present experiment was conducted to determine the optimal dietary Se level for meeting metabolic and functional Se requirements of broilers fed a corn-soybean meal diet from 22 to 42 d of age. A total of 336 Arbor Acres male broilers at 22 d old were randomly assigned to 1 of 6 treatments with 7 replicates and fed a basal corn-soybean meal diet (control, containing 0.014 mg Se/kg) and the basal diet supplemented with 0.10, 0.20, 0.30, 0.40, or 0.50 mg Se/kg from Na2SeO3 for 21 d. The results showed that the Se concentrations in plasma, liver, kidney, pancreas, breast and thigh muscles, the activity of glutathione peroxidase (GPX) in plasma, liver and kidney, the mRNA expression levels of Gpx4, selenoprotein (Seleno) h and Selenou in liver, Selenop and Selenoh in kidney, and the protein expression levels of GPX4 in the liver and kidney of broilers were affected (P

Abstract Background The current calcium (Ca) recommendation for broilers is primarily based on studies conducted more than 30 years ago with birds of markedly different productive potentials from those which exist today. And the response indicators in these studies are mainly growth performance and bone ash percentage. Therefore, the present study was carried out to investigate the effect of dietary Ca level on growth performance, serum parameters, bone characteristics and Ca metabolism-related gene expressions, so as to estimate dietary Ca requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age. Methods A total of 420 1-day-old Arbor Acres male broilers were randomly assigned to 1 of 7 treatments with 6 replicates (10 birds per cage) and fed the corn-soybean meal diets containing 0.60%, 0.70%, 0.80%, 0.90%, 1.00%, 1.10% or 1.20% Ca for 21 days. Each diet contained the constant non-phytate phosphorus content of about 0.39%. Results The average daily gain decreased linearly (P

Abstract Background Biochemical recurrence is defined as only rising CA-125 but no radiographic evidence of disease; noteworthily, it generally precedes the onset of clinical evidence. Now treatment strategies of biochemical recurrence ovarian cancer (OC) remain controversial. Apatinib as monotherapy or in combination with other chemotherapeutic agents has shown its effect in the treatment of some advanced malignancies. In our study, we focused on the efficacy of apatinib in recurrent OC, especially its clinical activity in biochemical-only recurrent OC patients. Methods We retrospectively analyzed clinical material of 41 recurrent patients who had received apatinib monotherapy or apatinib plus chemotherapy between June 2016 and August 2018. Apatinib was administered at a 500mg daily dose. Response was determined according to measurable disease or serum carbohydrate antigen (CA)-125 levels. Progression-free survival (PFS) was estimated by Kaplan–Meier method. Results All patients were evaluable, 19 (46.34%) had biochemical relapse and 22 (53.66%) had clinical relapse. The objective response rate (ORR) and disease control rate (DCR) in the overall population were 31.71% and 78.05%, respectively. The median PFS was 7 months (95% confidence interval 5.43–8.57). And in patients with biochemical-only relapse, the median PFS was 6 months, with ORR of 26.32% and DCR of 89.47%. Conclusions Apatinib is a well-tolerated and effective agent to delay clinical progression of patients with biochemical-only recurrent OC. More important, our study shows the promising prospect for treating OC patients with asymptomatic biochemical relapse.

Leiomyosarcoma of the uterus (ULMS) is a rare malignant tumor originating from embryonic mesenchymal cells. ULMS tends to metastasize to the lungs, lymph nodes, liver, and bone. Computed tomography three-dimensional (CT 3D) imaging is an advanced diagnostic technique that can track the vessels and their relationships with tumors and reveal the invasion of vessels, including small vessels, around tumors in any slice. Here, we describe a case in which ULMS extended to the retrohepatic inferior vena cava. To date, no report has described resection of metastatic ULMS of the vena cava through supplemental CT 3D imaging. Our patient presented with right lumbar abdominal pain as the main symptom. After using CT 3D reconstruction to accurately assess the relationship between the tumor and the surrounding organs and blood vessels before the operation, the operation was successfully completed through multidisciplinary surgical collaboration.

The high-pressure tritium leakage is a typical nuclear leakage accident, which can cause radioactive harm. The study aims at the tritium leakage problem in the high-pressure vessel of a nuclear reactor, installing a typical tritium leakage scenario and establishing the thermodynamic model of tritium leakage behavior which describes the transient process of tritium leakage. To analyze the effect of different ventilation scenes on tritium removal efficiency, the study sets up six different ventilation scenes and carries out the numerical simulation of tritium migration and mixing behavior based on Fluent. The result shows that different ventilation scenes will form different swirl distributions; the more complex the swirl distribution is, the faster the tritium concentration decreases. The shortest time required to decrease tritium concentration in space to the background level at the ventilation rate of 1 m3 s−1 is about 830 s and the longest time is about 1100 s. The overall tritium removal efficiency can be effectively improved when the swirl concentration distribution area is opposite to the exhaust outlet position. The setting method of ventilation is optimized, which provides technical support for the emergency treatment of tritium leakage accidents in the room.

In the process of tritium storage, the high-pressure storage vessel may be damaged due to accidental conditions, such as impact or drop, which results in tritium leakage. The tritium gas will be released and diffused into the space immediately, which inevitably causes radioactive hazards to personnel and the environment. Ventilation is considered to be the best emergency treatment method for a tritium leakage accident. According to the characteristics of fixed tritium amount and rapid release of tritium in the tritium leakage accident of the high-pressure storage vessel, the numerical simulation of high-pressure released tritium behavior in a confined space is carried out based on Fluent and compared with CATS test data. The error is no more than 5%. The ventilation conditions are optimized according to different ventilation positions and different ventilation rates. It is founded that the tritium removal efficiency when the vent is set on the roof is 30% higher than that on the side wall. The tritium concentration in a confined space can be reduced to the background level after ventilation for 30 min at a rate of 0.5 m3 s−1. The optimal recovery time of the environment is given, which provides technical support for the emergency treatment of the tritium leakage accident of the high-pressure storage vessel.

As a non-destructive testing and analysis technology, the neutron multiplicity measurement method plays an important role in the field of arms control verification. Great progress has been achieved in the fast neutron multiplicity measurement of plutonium, while there are few studies that have been conducted in the fast neutron multiplicity measurement of uranium. In this study, a set of fast neutron multiplicity measurement devices based on the BC501A liquid scintillation detector was built, and two small mass uranium samples with low density were studied. It is found that the change of the total neutron counting rate is irregular, and the coincidence counting rate will increase linearly with the mass. It is due to the energy loss caused by the scatter of the Am-Li source neutron with the sample, resulting in part of the neutron energy below the threshold. A simulation detection system is built by Geant4 to verify the reliability. On this basis, four kinds of packaging materials such as steel, copper, aluminum, and graphite were studied. The measurement results of different materials were compared and analyzed. The thickness of the material was studied, and the functional relationship curve was fitted. In this study, the fast neutron multiplicity measurement of small-mass and low-density uranium samples was carried out and verified. The influence of different packaging materials on the multiplicity measurement was analyzed, which is of great significance for the development of the fast neutron multiplicity measurement technology.

An experiment was conducted to investigate the effect of dietary calcium (Ca) or phosphorus (P) deficiency on bone development and related Ca or P metabolic utilization parameters of broilers. A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates of 18 birds per replicate in a completely randomized design. A 2 (Ca levels: 1.00 and 0.35%) × 2 (nonphytate P [NPP] levels: 0.45 and 0.23%) factorial arrangement of treatments was adopted in the 21-day trial. The 4 treatments were the Ca- and P-adequate diet (1.00% Ca + 0.45% NPP), the Ca-deficient diet (0.35% Ca + 0.45% NPP), the P-deficient diet (1.00% Ca + 0.23% NPP), and the Ca- and P-deficient diet (0.35% Ca + 0.23% NPP). The greatest impact on tibia bone mineral density, bone breaking strength, and ash content was in the P-deficient diets, especially in broilers fed with the Ca-adequate diet, whereas adequate P and reduced Ca reduced (P < 0.05) these parameters compared with adequate Ca and P, but not to the same level as P deficiency. Furthermore, dietary Ca or P deficiency, especially adequate Ca and P deficiency decreased (P < 0.05) serum P, 25-hydroxyvitamin D3 (25-OHD3) contents, and tibia ash Ca and P contents but increased (P < 0.05) the serum Ca content and tibia alkaline phosphatase (ALP) activity compared with adequate Ca and P. The results from this study indicated that the bone development and Ca or P metabolic utilization parameters of broilers were the most sensitive ones to dietary P deficiency, followed by dietary Ca deficiency or Ca and P deficiencies. Dietary P deficiency impaired the bone development by increasing serum Ca content and tibia ALP activity but decreasing serum P, 25-OHD3 contents, and tibia ash Ca and P contents of broilers. Dietary Ca deficiency impaired bone development by increasing serum Ca content, tibia ALP activity, and tibia ash P content but decreasing serum P, 25-OHD3 contents, and tibia ash Ca content of broilers.

Fast-Neutron Multiplicity Counter based on Liquid Scintillator Detector can directly measure the fast neutron multiplicity emitted by UO2 rod. HPGe gamma spectrometer; which has superior energy resolution; is routinely used for the gamma energy spectrum measurement. Combing Fast-Neutron Multiplicity Counter and HPGe γ-spectrometer, the n/γ joint measurement system is developed. The fast neutron multiplicity and gamma energy spectrum of UO2 rod assemblies under different conditions are measured by the n/γ joint measurement system. The induced fission rate and the 235U abundance, thereby the 235U mass; can be obtained for UO2 rod assemblies. The 235U mass deviation of the measured value from the reference value is less than 5%. The results show that the n/γ joint measurement system is effective and applicable in the measurement of the 235U mass in samples. Keywords: Fast-neutron multiplicity counter, n/γ joint measurement system, Liquid scintillator detector, HPGe γ spectrometer, UO2 rod assembly, 235U mass

In the measurement of fast neutron multiplicity, the multiplicity counting rates of neutrons, including singles, doubles, and triplets, are often substituted into the measurement equation to solve quality problems. To simplify the solution process and directly obtain the sample quality through S, D, and T, a neural network and multivariate nonlinear fitting are used for analysis. First, multiple sets of data are measured through a detection system built with Geant4. After the training of the back propagation neural network, the corresponding relationship between S, D, T, and m is established. It is verified that there are different degrees of discrepancy between the predicted values of the neural network and the simulated and theoretical values. To improve the accuracy of predictions, genetic algorithm optimization and M coefficient correction are introduced. To analyze the stability of the neural network model, a 10% error perturbation is introduced for S, D, and T. The double rate has the greatest influence on the deviation of the predicted value, indicating that the double rate is the key parameter in the analysis of neutron multiplicity. On this basis, a functional relationship is obtained through multivariate nonlinear fitting, and the validation of the fitting equation is verified by simplifying the fast neutron multiplicity measurement technology equation.

Network virtualization aims to realize the decoupling of hardware and software by virtualization,and share the resource according to service size by cloudification,thus supporting the telecom business with features of high flexibility,maintainability,usability and immediate return of investment.The virtualization architecture and key technologies of NFV were introduced,and the typical NFV application scenarios for telecom operators were presented to promote the research and implementation of telecom network virtualization technology.

A monitoring system solution of supporting multi-datacenter and multiple private cloud environments were proposed.In order to achieve load balancing and easy scale-out，the unified monitoring system adopted a distributed framework and encapsulated Zabbix API and OpenStack API.This solution was significant for the study of similar distributed cluster scheduling system.

Based on the current research on mobile CDN and core network virtualization, the method of deploying CND service nodes on virtualized EPC resources were discussed. First, the deployment solution of MCDN under the LTE was analyzed, as well as the virtualization solution of core net. Then a MCDN integration architecture was proposed on the basis of virtualized EPC resources, which can archive resources sharing and system expansion of MCDN service nodes by using virtualization technology and improve the reliability and efficiency of LTE and CDN.

The NFV and SDN were introduced, the current work in network virtualization was analyzed and its requirements were concluded. Aimed at network architecture of mobile core network, and then the architecture of core network virtualization were proposed and brief introduction was made which was very import to guide the evolution of core network.

In order to obtain a deeper understanding of the regenerative cooling process of scramjet engines, in this paper, a numerical investigation on the supercritical convective heat transfer of n-decane in horizontal circular tubes was conducted, based on a complete set of conservation equations and the Renormalization group (RNG) k–ε turbulence model with enhanced wall treatment. The present study mainly focuses on the heat transfer deterioration (HTD) phenomenon, including the mechanism and critical conditions for the onset of HTD. Moreover, the applicability of some conventional heat transfer empirical correlations was analyzed and compared, thus providing guidance for the Nusselt number predictions in the cooling channels. Results indicate that under the compositive conditions of low pressure and high heat flux, two types of HTD phenomena could occur when the wall and bulk fluid temperatures are near the pseudo-critical temperature, owing to the abnormal distributions of near-wall turbulent kinetic energy and radial velocity, respectively. Increasing the pressure would effectively alleviate and eliminate the HTD. A comparison of numerical results with those obtained with different empirical expressions shows that the Bae-Kim expression provides the best agreement, especially when HTD occurs. Furthermore, a new correction for critical heat flux of HTD has been successfully developed.

This study investigated the rebound behavior of SiO2 particles normally impacting a planar surface under different temperature conditions. The system has been characterized for an aerosol inlet temperature range of 20–190 °C, flow velocities of 0–20 ms−1, and an impaction surface temperature range of 20–140 °C. For the first time, while keeping the same temperature gradient from the high- to low-temperature regions, the influences of varying temperature on the rebound behavior of SiO2 particles normally impacting a plane surface were examined. At increased temperatures, the plastic deformation increases and the coefficient of restitution reduces. The critical velocity is between 0.542 and 0.546 m/s under condition 1 (the carrier gas temperature (Tg) and the impaction surface temperature (TW) remain at room temperature of 20 °C), which increases to between 0.829 and 0.847 m/s under condition 4 (Tg and TW remain at temperatures of 190 °C and 140 °C, respectively). The experimental results show that the critical velocity increases with increasing temperature.

In this study, the antioxidant components in co-culture of Chlorella pyrenoidosa and Yarrowia lipolytica (3:1 ratio) were confirmed as trypsin-hydrolyzed peptides (EHPs). The EHPs were composed of 836 different peptides with molecular weights ranging from 639 to 3531 Da and were mainly composed of hydrophobic amino acids (48.1%). These peptides showed remarkable protective effects against oxidative stress in HepG2, which may be attributed to their structures. Furthermore, the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were significantly lower in the peptide-treated group than in the control group, suggesting that the antioxidant enzyme-coding genes were not activated. The EC50 value of three peptides in the EHPs were in the order of AGYSPIGFVR (0.04 ± 0.002 mg/mL) > VLDELTLAR (0.09 ± 0.001 mg/mL) > LFDPVYLFDQG (0.41 ± 0.03 mg/mL); these results agreed with the prediction of the model (R2 > 0.9, Q2 > 0.5). Thus, EHPs show potential as potent new antioxidant agents.

The rebound behavior of fly ash particles normally impacting a planar surface is investigated by using a dynamic model. The three forms of soft sphere physical model are obtained using static/quasi-static contact mechanics and energy dissipation theory. The influences of the particle size, the incident velocity of the particle on the damping coefficient and the impact contact time are all examined. We also predict the critical velocity for three particle sizes. It is found that the variation of the damping coefficient (η) with the normal incident velocity (vin) can be roughly divided into the three parts. In the first part, η decreases with increasing vin. In the second part, η is little changed with increasing vin. In the third part, η rapidly increases with increasing vin. For smaller impact velocities, the viscoelastic effect plays a dominant role in the impact process, while for higher incident velocities; the energy dissipation depends mainly on plastic deformation. In addition, the critical velocity shows a distinct dependence on the particle size. Finally, the contact displacement-contact time curves are examined. The work provides a solid basis for the development of a discrete-element-method approach to study ash deposition.

This paper presents the results of a comprehensive program of experiments in which fly ash particles were impacted under controlled conditions against a flat steel surface. The overall aim of these experiments was to gain an understanding of the ash deposition process in a pulverized coal boiler system. A continuous nitrogen flow carrying fly ash particles was used to examine the effect of particle incident velocity and particle diameter (dp) on the normal restitution coefficient, and of the particle diameter on the critical velocity. The effect of the incident normal velocity and particle diameter on the normal restitution coefficient was also examined. The results show that the normal restitution coefficient increases rapidly with increasing incident velocity when this incident velocity is less than the yield velocity, and rapidly decreases with increasing incident velocity when the incident velocity is greater than the yield velocity. The critical velocity, determined solely by the first-contact energy loss, is proportional to dp−5/6 and therefore becomes larger for smaller particles. For instance, in the present work, the velocity vc of a particle with diameter of 85 μm is 0.19 m/s, which increases to 0.42 m/s for particles with a diameter of 65 μm.

As one kind of the main resources of cloud computing，virtual resource sustains sorts of cloud services，and an effective resource scheduling mechanism helps to balance the load and improve the resource utility rate.Focusing on virtual resource of cloud，a resource scheduling mechanism to achieve load balancing was proposed.Triggered by an incoming job，the scheduler checked the instant state of resource pool first，and then took interrelated strategies.

An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1-21 days and 61.0% during 22-42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P>0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the digesta of the ileum and rectum, heart, liver, kidney, and breast or thigh muscles of broilers fed with the PTC diets. It was concluded that PTC had no adverse effect on the quantity and diversity of gut microorganisms; Transgenic phyA2 DNA or protein was rapidly degraded in the intestinal tract and was not transferred to the tissues of broilers.

Porcine rotaviruses (PoRVs) cause severe economic losses in the swine industry. P[7] and P[23] are the predominant genotypes circulating on farms, but no vaccine is yet available. Here, we developed a bivalent subunit PoRV vaccine using truncated versions (VP4*) of the VP4 proteins from P[7] and P[23]. The vaccination of mice with the bivalent subunit vaccine elicited more robust neutralizing antibodies (NAbs) and cellular immune responses than its components, even at high doses. The bivalent subunit vaccine and inactivated bivalent vaccine prepared from strains PoRVs G9P[7] and G9P[23] were used to examine their protective efficacy in sows and suckling piglets after passive immunization. The immunized sows showed significantly elevated NAbs in the serum and colostrum, and the suckling piglets acquired high levels of sIgA antibodies from the colostrum. Challenging subunit-vaccinated or inactivated-vaccinated piglets with homologous virulent strains did not induce diarrhea, except in one or two piglets, which had mild diarrhea. Immunization with the bivalent subunit vaccine and inactivated vaccine also alleviated the microscopic lesions in the intestinal tissues caused by the challenge with the corresponding homologous virulent strain. However, all the piglets in the challenged group displayed mild to watery diarrhea and high levels of viral shedding, whereas the feces and intestines of the piglets in the bivalent subunit vaccine and inactivated vaccine groups had lower viral loads. In summary, our data show for the first time that a bivalent subunit vaccine combining VP4*P[7] and VP4*P[23] effectively protects piglets against the diarrhea caused by homologous virulent strains. [ABSTRACT FROM AUTHOR]