1. Cryo-EM structure of human hexameric MCM2-7 complex.
- Author
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Xu N, Lin Q, Tian H, Liu C, Wang P, Suen CM, Yang H, Xiang Y, and Zhu G
- Abstract
The central step in the initiation of eukaryotic DNA replication is the loading of the minichromosome maintenance 2-7 (MCM2-7) complex, the core of the replicative DNA helicase, onto chromatin at replication origin. Here, we reported the cryo-EM structure of endogenous human single hexameric MCM2-7 complex with a resolution at 4.4 Å, typically an open-ring hexamer with a gap between Mcm2 and Mcm5. Strikingly, further analysis revealed that human MCM2-7 can self-associate to form a loose double hexamer which potentially implies a novel mechanism underlying the MCM2-7 loading in eukaryote. The high-resolution cryo-EM structure of human MCM2-7 is critical for understanding the molecular mechanisms governing human DNA replication, especially the MCM2-7 chromatin loading and pre-replicative complex assembly., Competing Interests: The authors declare no competing interests., (© 2022 The Authors.)
- Published
- 2022
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