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Your search keyword '"Sudhakaran, Indulekha P."' showing total 19 results
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19 results on '"Sudhakaran, Indulekha P."'

5. α-Synuclein phosphorylation at serine 129 occurs after initial protein deposition and inhibits seeded fibril formation and toxicity

Author

Ghanem, Simona S., primary, Majbour, Nour K., additional, Vaikath, Nishant N., additional, Ardah, Mustafa T., additional, Erskine, Daniel, additional, Jensen, Nanna Møller, additional, Fayyad, Muneera, additional, Sudhakaran, Indulekha P., additional, Vasili, Eftychia, additional, Melachroinou, Katerina, additional, Abdi, Ilham Y., additional, Poggiolini, Ilaria, additional, Santos, Patricia, additional, Dorn, Anton, additional, Carloni, Paolo, additional, Vekrellis, Kostas, additional, Attems, Johannes, additional, McKeith, Ian, additional, Outeiro, Tiago F., additional, Jensen, Poul Henning, additional, and El-Agnaf, Omar M. A., additional

Published
2022
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6. Novel engineered nanobodies specific for N‐terminal region of alpha‐synuclein recognize Lewy‐body pathology and inhibit in‐vitro seeded aggregation and toxicity

Author

Hmila, Issam, primary, Vaikath, Nishant N., additional, Majbour, Nour K., additional, Erskine, Daniel, additional, Sudhakaran, Indulekha P., additional, Gupta, Vijay, additional, Ghanem, Simona S., additional, Islam, Zeyaul, additional, Emara, Mohamed M., additional, Abdesselem, Houari B., additional, Kolatkar, Prasanna R., additional, Achappa, Devaya K., additional, Vinardell, Tatiana, additional, and El‐Agnaf, Omar M. A., additional

Published
2022
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7. Plasticity of local GABAergic interneurons drives olfactory habituation

Author

Das, Sudeshna, Sadanandappa, Madhumala K., Dervan, Adrian, Larkin, Aoife, Lee, John Anthony, Sudhakaran, Indulekha P., Priya, Rashi, Heidari, Raheleh, Holohan, Eimear E., Pimentel, Angel, Gandhi, Avni, Ito, Kei, Sanyal, Subhabrata, Wang, Jing W., Rodrigues, Veronica, and Ramaswami, Mani

Published
2011

9. Investigating the presence of doubly phosphorylated α‐synuclein at tyrosine 125 and serine 129 in idiopathic Lewy body diseases

Author

Fayyad, Muneera, primary, Erskine, Daniel, additional, Majbour, Nour K., additional, Vaikath, Nishant N., additional, Ghanem, Simona S., additional, Sudhakaran, Indulekha P., additional, Abdesselem, Houari, additional, Lamprokostopoulou, Agaristi, additional, Vekrellis, Kostas, additional, Morris, Christopher M., additional, Attems, Johannes, additional, and El‐Agnaf, Omar M. A., additional

Published
2020
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11. RNP-Granule Assembly via Ataxin-2 Disordered Domains Is Required for Long-Term Memory and Neurodegeneration

Author

Bakthavachalu, Baskar, primary, Huelsmeier, Joern, additional, Sudhakaran, Indulekha P., additional, Hillebrand, Jens, additional, Singh, Amanjot, additional, Petrauskas, Arnas, additional, Thiagarajan, Devasena, additional, Sankaranarayanan, M., additional, Mizoue, Laura, additional, Anderson, Eric N., additional, Pandey, Udai Bhan, additional, Ross, Eric, additional, VijayRaghavan, K., additional, Parker, Roy, additional, and Ramaswami, Mani, additional

Published
2018
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12. Dysregulation of mRNA Localization and Translation in Genetic Disease

Author

Massachusetts Institute of Technology. Department of Biology, Taliaferro, Jefferson Matthew, Wang, Eric T., Lee, Ji-Ann, Sudhakaran, Indulekha P., Rossoll, Wilfried, Gross, Christina, Moss, Kathryn R., Bassell, Gary J., Massachusetts Institute of Technology. Department of Biology, Taliaferro, Jefferson Matthew, Wang, Eric T., Lee, Ji-Ann, Sudhakaran, Indulekha P., Rossoll, Wilfried, Gross, Christina, Moss, Kathryn R., and Bassell, Gary J.

Abstract

RNA-binding proteins (RBPs) acting at various steps in the post-transcriptional regulation of gene expression play crucial roles in neuronal development and synaptic plasticity. Genetic mutations affecting several RBPs and associated factors lead to diverse neurological symptoms, as characterized by neurodevelopmental and neuropsychiatric disorders, neuromuscular and neurodegenerative diseases, and can often be multisystemic diseases. We will highlight the physiological roles of a few specific proteins in molecular mechanisms of cytoplasmic mRNA regulation, and how these processes are dysregulated in genetic disease. Recent advances in computational biology and genomewide analysis, integrated with diverse experimental approaches and model systems, have provided new insights into conserved mechanisms and the shared pathobiology of mRNA dysregulation in disease. Progress has been made to understand the pathobiology of disease mechanisms for myotonic dystrophy, spinal muscular atrophy, and fragile X syndrome, with broader implications for other RBP-associated genetic neurological diseases. This gained knowledge of underlying basic mechanisms has paved the way to the development of therapeutic strategies targeting disease mechanisms.

Published
2017

18. Plasticity of Recurrent Inhibition in the Drosophila Antennal Lobe.

Author

Sudhakaran, Indulekha P., Holohan, Eimear E., Osman, Sahar, Rodrigues, Veronica, Vijay Raghavan, K., and Ramaswami, Mani

Subjects
*NEUROPLASTICITY, *DROSOPHILA, *INSECT interneurons, *OLFACTORY cortex, *SENSORY neurons, *HABITUATION (Neuropsychology), *NEURAL stimulation
Abstract

Recurrent inhibition, wherein excitatory principal neurons stimulate inhibitory interneurons that feedback on the same principal cells, occurs ubiquitously in the brain. However, the regulation and function of recurrent inhibition are poorly understood in terms of the contributing interneuron subtypes as well as their effect on neural and cognitive outputs. In the Drosophila olfactory system, odorants activate olfactory sensory neurons (OSNs), which stimulate projection neurons (PNs) in the antennal lobe. Both OSNs and PNs activate local inhibitory neurons (LNs) that provide either feedforward or recurrent/feedback inhibition in the lobe. During olfactory habituation, prior exposure to an odorant selectively decreases the animal's subsequent response to the odorant. We show here that habituation occurs in response to feedback from PNs. Output from PNs is necessary for olfactory habituation and, in the absence of odorant, directPN activation is sufficient to induce the odorant-selective behavioral attenuation characteristic of olfactory habituation. PN-induced habituation occludes further odor-induced habituation and similarly requires GABAARs and NMDARs in PNs, as well as VGLUT and cAMP signaling in the multiglomerular inhibitory local interneurons (LN1) type of LN. Thus, PN output is monitored by an LN subtype whose resultant plasticity underlies behavioral habituation. We propose that recurrent inhibitory motifs common in neural circuits may similarly underlie habituation to other complex stimuli. [ABSTRACT FROM AUTHOR]

Published
2012
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19. Production of α-Synuclein Fibrillar-Specific scFv from Inclusion Bodies.

Author

Gupta V, Hmila I, Vaikath NN, Sudhakaran IP, and El-Agnaf OMA

Subjects
Escherichia coli genetics, Recombinant Proteins, Inclusion Bodies, alpha-Synuclein, Single-Chain Antibodies genetics
Abstract

Recombinant antibody fragments such as Fab, scFvs, and diabodies against α-syn have become a viable alternative to the conventional full-length antibodies in immunotherapeutic approaches due to their benefits which include smaller size, higher stability, specificity, and affinity. However, the majority of recombinant antibody fragments typically express as inclusion bodies (IBs) in E. coli, which makes their purification incredibly difficult. Here, we describe a method involving a mild solubilizing protocol followed by slow on-column refolding to purify active single-chain variable fragment (scFv-pF) antibody that can recognize the pathogenic α-syn fibrils., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2023
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