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The environment plays a critical role in the development, dissemination, and transmission of antimicrobial resistance (AMR). Pharmaceuticals and personal care products (PPCPs) enter the environment through direct application to the environment and through anthropogenic pollution. Although there is a growing body of evidence defining minimal selective concentrations (MSCs) of antibiotics and the role antibiotics play in horizontal gene transfer (HGT), there is limited evidence on the role of non-antibiotic PPCPs. Existing data show associations with the development of resistance or effects on bacterial growth rather than calculating selective endpoints. Research has focused on laboratory-based systems rather than in situ experiments, although PPCP concentrations found throughout wastewater, natural water, and soil environments are often within the range of laboratory-derived MSCs and at concentrations shown to promote HGT. Increased selection and HGT of AMR by PPCPs will result in an increase in total AMR abundance in the environment, increasing the risk of exposure and potential transmission of environmental AMR to humans. There is some evidence to suggest that humans can acquire resistance from environmental settings, with water environments being the most frequently studied. However, because this is currently limited, we recommend that more evidence be gathered to understand the risk the environment plays in regard to human health. In addition, we recommend that future research efforts focus on MSC-based experiments for non-antibiotic PPCPS, particularly in situ, and investigate the effect of PPCP mixtures on AMR.

Volcano monitoring requires simple techniques to rapidly identify the cause of volcanic unrest. The so-called RSAM (real-time seismic amplitude measurements) technique, used in many observatories, is a good example of extracting information from seismograms with minimal processing. Built on a similar principle, the more recent seismic amplitude ratio analysis (SARA) technique allows locating migrating seismicity at high frequency (> 2 Hz, e.g. due to dike intrusions) under certain assumptions. However, such analysis generally requires a dense distribution of stations close to the seismic sources (depending on the magnitude) and/or station sites undisturbed by human activity. In a more straightforward and qualitative approach, computing amplitude ratios between station pairs can also allow for the detection of temporal and (2D) spatial changes of volcanic activity. In this work, we adopt such a simplified approach of SARA in order to characterize seismic tremors originating from two open-vent neighboring volcanoes, Nyiragongo and Nyamulagira, in the Virunga Volcanic Province (VVP) in the Democratic Republic of the Congo (DRC). In contrast with previous studies, we focus here on the low-frequency band (0.3–1 Hz), free from anthropogenic noise and sensitive to shallow volcanic tremors linked to intermittent or permanent intra-crater eruptive activity recorded through the large-aperture local network. We apply for the first time the SARA methodology for volcanic sources predominantly generating surface waves and propagating over long distances. The analysis is performed on more than two years of continuous seismic data. Seismic amplitude analysis in this frequency band is strongly influenced by the short-period microseisms originating from nearby Lake Kivu. Despite this diurnal to seasonal amplitude variability, SARA successfully detects continuous volcanic tremor activity and its arrest at both volcanoes. In light of these findings, we discuss the applicability of the, SCOPUS: ar.j, info:eu-repo/semantics/published

The environment plays a critical role in the development, dissemination, and transmission of antimicrobial resistance (AMR). Pharmaceuticals and personal care products (PPCPs) enter the environment through direct application to the environment and through anthropogenic pollution. Although there is a growing body of evidence defining minimal selective concentrations (MSCs) of antibiotics and the role antibiotics play in horizontal gene transfer (HGT), there is limited evidence on the role of non-antibiotic PPCPs. Existing data show associations with the development of resistance or effects on bacterial growth rather than calculating selective endpoints. Research has focused on laboratory-based systems rather than in situ experiments, although PPCP concentrations found throughout wastewater, natural water, and soil environments are often within the range of laboratory-derived MSCs and at concentrations shown to promote HGT. Increased selection and HGT of AMR by PPCPs will result in an increase in total AMR abundance in the environment, increasing the risk of exposure and potential transmission of environmental AMR to humans. There is some evidence to suggest that humans can acquire resistance from environmental settings, with water environments being the most frequently studied. However, because this is currently limited, we recommend that more evidence be gathered to understand the risk the environment plays in regard to human health. In addition, we recommend that future research efforts focus on MSC-based experiments for non-antibiotic PPCPS, particularly in situ, and investigate the effect of PPCP mixtures on AMR. Environ Toxicol Chem 2022;00:1-14. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

The main objective of this study was to investigate the antibiotic resistance (AR) levels in wastewater(WW) and the impact on the receiving river. Samples were collected once per season over one year in theWWof a hospital, in the raw and treated WWof two wastewater treatment plants (WWTPs), as well asupstream and downstream from the release ofWWTPs effluents into the Zenne River (Belgium). Culturedependentmethods were used to quantify Escherichia coli and heterotrophic bacteria resistant toamoxicillin, sulfamethoxazole, nalidixic acid and tetracycline. Six antibiotic resistance genes (ARGs) werequantified in both particle-attached (PAB) and free-living (FLB) bacteria. Our results showed thatWWTPsefficiently removed antibiotic resistant bacteria (ARB) regardless of its AR profile. The ARGs levels werethe highest in the hospital WWand were significantly reduced in both WWTPs. However, ARB and ARGsabundances significantly increased into the Zenne River downstream from the WWTPs outfalls. Thevariation in the relative abundance of ARGs through WW treatment differed depending on the WWTP,fraction, and gene considered. The sul1 and sul2 genes in PAB fraction showed significantly higherrelative abundances in the effluent compared to the influent of both WWTPs. This study demonstratedthat WWTPs could be hotspots for AR spread with significant impacts on receiving freshwater ecosystems.This was the first comprehensive study investigating at the same time antibiotics occurrence, fecal bacteria indicators, heterotrophic bacterial communities, and ARGs (distinguishing PAB and FLB) to assess AR levels in WW and impacts on the receiving river., SCOPUS: ar.j, info:eu-repo/semantics/published

Urban rivers are impacted ecosystems which may play an important role as reservoirs for antibiotic-resistant (AR) bacteria. The main objective of this study was to describe the prevalence of antibiotic resistance along a sewage-polluted urban river. Seven sites along the Zenne River (Belgium) were selected to study the prevalence of AR Escherichia coli and freshwater bacteria over a 1-year period. Culture-dependent methods were used to estimate E. coli and heterotrophic bacteria resistant to amoxicillin, sulfamethoxazole, nalidixic acid and tetracycline. The concentrations of these four antibiotics have been quantified in the studied river. The antibiotic resistance genes (ARGs), sul1, sul2, tetW, tetO, blaTEM and qnrS were also quantified in both particle-attached (PAB) and free-living (FLB) bacteria. Our results showed an effect of treated wastewaters release on the spread of antibiotic resistance along the river. Although an increase in the abundance of both AR E. coli and resistant heterotrophic bacteria was observed from upstream to downstream sites, the differences were only significant for AR E. coli. A significant positive regression was also found between AR E. coli and resistant heterotrophic bacteria. The concentration of ARGs increased from upstream to downstream sites for both particle-attached (PAB) and free-living bacteria (FLB). Particularly, a significant increase in the abundance of four among six ARGs analyzed was observed after crossing urban area. Although concentrations of tetracycline significantly correlated with tetracycline resistance genes, the antibiotic levels were likely too low to explain this correlation. The analysis of ARGs in different fractions revealed a significantly higher abundance in PAB compared to FLB for tetO and sul2 genes. This study demonstrated that urban activities may increase the spread of antibiotic resistance even in an already impacted river., SCOPUS: ar.j, info:eu-repo/semantics/published