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Staphylococcus aureus is a significant bacterium that causes substantial economic losses in the livestock sector and poses life-threatening risks to both humans and animals. This study aimed to explore the prevalence, susceptibility to antimicrobials, and profiles of virulence and antimicrobial resistance (AMR) genes in S. aureus isolated from nasal swabs and lung tissues of sheep and goats exhibiting symptoms of respiratory disease. A total of 194 samples were examined, resulting in the isolation and confirmation of S. aureus in 65 samples, indicating an overall prevalence of 33.5%. These isolates were further subjected to an AMR assay. Among the representative isolates (37), sensitivity was observed to chloramphenicol and ceftriaxone. Conversely, penicillin showed the lowest efficacy, with 83.8% of isolates demonstrating resistance, followed closely by amoxiclav, which exhibited resistance in 75.7% of isolates. Close to threequarters of isolates carried at least one AMR gene. Virulence genes were identified in 67.6% of S. aureus isolates, with coa and lukpv detected in 37.8% and arcA in 32.4% of isolates. Additionally, mecA and vatC were present in 54.05%, vatB in 43.25% and aphD in 18.91% of S. aureus isolates. A substantial 91.9% of isolates exhibited multidrug resistance, and MAR indices exceeding 0.2 were recorded in 86.5% of S. aureus isolates, indicating a high public health risk. These findings underscore the importance of prioritizing infections caused by S. aureus, necessitating heightened attention from both veterinarians and healthcare workers in migratory communities. Furthermore, the regulated and judicious use of antimicrobials is crucial to mitigate the risks associated with antimicrobial resistance in these settings.

A 34-day-old, male Bully pup was presented in veterinary clinics with an uncertain anamnestic history of anorexia, lethargy, abdominal pain, and discomfort. On clinical examination, the pup had pale conjunctiva with a pot-bellied appearance and expelled cream-colored roundworms in feces. Blood and fecal samples were collected and analyzed. Hemoglobin and PCV values were lower, depicting anemia. A qualitative and quantitative examination of the fecal sample was carried out and results showed infection with Toxocara canis with fecal egg count (FEC) of 3200/gram of feces. Gross and light microscopic examination revealed the presence of adults of Toxocara canis with distinctive morphological features. Apropos, the pup was treated with a combination of pyrantel pamoate and fenbendazole orally @10mg/kg body weight, repeated after 14 days. The pup recovered successfully, as evidenced by decreased FECs, increased weight gain, and a high hematocrit score.

• multocida contain various surface-associated antigens that could be used as a target for both therapeutics as well as diagnostics. The current study was planned to select ligands using Ph.D.-12 phage display library. This library was amplified and subjected to the alternate selection/subtraction methodology of biopanning using the suspension method in which alternate rounds of positive selection against P. multocida and negative selection against Haemophilus influenzae and Actinobacillus lignieresii were performed. After completing biopanning, out of 48 selected phages, 16 clonal phages were selected for indirect phage ELISA to check their binding efficiency with P. multocida. Out of these 16, five clonal phages bound their target with high intensity giving higher OD values at 450 nm and their binding efficiency was compared with closely related Actinobacillus lignieresii and Hemophilus influenzae using 107 pfu/ml at 450 nm wavelength which was found to be less against these bacteria.

Coronaviruses are single-stranded RNA viruses that affect humans and a wide variety of animal species, including livestock, wild animals, birds, and pets. These viruses have an affinity for different tissues, such as those of the respiratory and gastrointestinal tract of most mammals and birds and the hepatic and nervous tissues of rodents and porcine. As coronaviruses target different host cell receptors and show divergence in the sequences and motifs of their structural and accessory proteins, they are classified into groups, which may explain the evolutionary relationship between them. The interspecies transmission, zoonotic potential, and ability to mutate at a higher rate and emerge into variants of concern highlight their importance in the medical and veterinary fields. The contribution of various factors that result in their evolution will provide better insight and may help to understand the complexity of coronaviruses in the face of pandemics. In this review, important aspects of coronaviruses infecting livestock, birds, and pets, in particular, their structure and genome organization having a bearing on evolutionary and zoonotic outcomes, have been discussed.

Aim: The study was designed to measure the seroprevalence of viral and bacterial diseases: Infectious bovine rhinotracheitis, bovine viral diarrhea, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, brucellosis, and paratuberculosis among bovine of Himachal Pradesh during the year 2013-2015. Materials and Methods: The serum samples were collected from seven districts of state, namely, Bilaspur, Kangra, Kinnaur, Lahul and Spiti, Mandi, Sirmour, and Solan. The samples were screened using indirect ELISA kits to measure the seroprevalence of viral and bacterial diseases. Results: The overall seroprevalence of infectious bovine rhinotracheitis was 24.24%, bovine viral diarrhea 1.52%, bovine leukemia 9.09%, bovine parainfluenza 57.58%, bovine respiratory syncytial disease 50%, brucellosis 19.69%, and paratuberculosis 9.09% in Himachal Pradesh. The seroprevalence of bovine rhinotracheitis, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, and paratuberculosis in the state varied significantly (p0.01). Multiple seropositivity has been observed in this study. Bovine parainfluenza virus 3 was observed commonly in mixed infection with almost all viruses and bacteria under study. Conclusion: The viral and bacterial diseases are prevalent in the seven districts of Himachal Pradesh investigated in the study. Therefore, appropriate management practices and routine vaccination programs should be adopted to reduce the prevalence of these diseases.

Background. Multiple sclerosis (MS) is a chronic debilitating disorder characterized by persisting damage to the brain caused by autoreactive leukocytes. Leukocyte activation is regulated by cytokines, which are readily detected in MS serum and cerebrospinal fluid (CSF). Objective. Serum and CSF levels of forty-five cytokines were analyzed to identify MS diagnostic markers. Methods. Cytokines were analyzed using multiplex immunoassay. ANOVA-based feature and Pearson correlation coefficient scores were calculated to select the features which were used as input by machine learning models, to predict and classify MS. Results. Twenty-two and twenty cytokines were altered in CSF and serum, respectively. The MS diagnosis accuracy was ≥92% when any randomly selected five of these biomarkers were used. Interestingly, the highest accuracy (99%) of MS diagnosis was demonstrated when CCL27, IFN-γ, and IL-4 were part of the five selected cytokines, suggesting their important role in MS pathogenesis. Also, these binary classifier models had the accuracy in the range of 70-78% (serum) and 60-69% (CSF) to discriminate between the progressive (primary and secondary progressive) and relapsing-remitting forms of MS. Conclusion. We identified the set of cytokines from the serum and CSF that could be used for the MS diagnosis and classification.

Multiple sclerosis (MS) is an immune inflammatory disease, where the underlying etiological cause remains elusive. Multiple triggering factors have been suggested, including environmental, genetic and gender components. However, underlying infectious triggers to the disease are also suspected. There is an increasing abundance of evidence supporting a viral etiology to MS, including the efficacy of interferon therapy and over-detection of viral antibodies and nucleic acids when compared with healthy patients. Several viruses have been proposed as potential triggering agents, including Epstein–Barr virus, human herpesvirus 6, varicella–zoster virus, cytomegalovirus, John Cunningham virus and human endogenous retroviruses. These viruses are all near ubiquitous and have a high prevalence in adult populations (or in the case of the retroviruses are actually part of the genome). They can establish lifelong infections with periods of reactivation, which may be linked to the relapsing nature of MS. In this review, the evidence for a role for viral infection in MS will be discussed with an emphasis on immune system activation related to MS disease pathogenesis.

Post-translational modifications to histone tails contribute to the three-dimensional structure of chromatin and play an important role in determining the relative expression of nearby genes. One such modification is symmetric di-methylation of arginine residues, which may exhibit different effects on gene expression including blocking the binding of transcriptional activators, or recruiting repressive effector molecules. Recent ChIP-Seq studies have demonstrated the importance of cross-talk between different histone modifications in gene regulation. Thus, to acquire a comprehensive understanding of the combined efforts of these epigenetic marks, ChIP-Seq must be utilized for identifying specific enrichment on the chromatin. Tumorigenic herpesvirus KSHV, employs epigenetic mechanisms for gene regulation, and by evaluating relative abundance of multiple histone modifications in a thorough, unbiased way, using ChIP-Seq, we can get a superior insight concerning the complex mechanisms of viral replication and pathogenesis.