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This research has succeeded in developing Mn0.25Fe2.75O4/Ag/OA/DMSO ferrofluid based on natural sand and sunflower seed oil as antibacterial agents. The ferrofluidmethods and.25Fe2.75O4/Ag, was prepared by co-precipitation and reduction methods, and characterized by XRD and SEM to determine the crystal structure, morphology, and particle size. Furthermore, the functional groups of ferrofluid Mn0.25Fe2.75O4/Ag/OA/DMSO are known from the FTIR test results. The antibacterial activity of ferrofluid has also been characterized using a good method to determine the diameter of the zone of inhibition of bacterial growth. XRD diffraction pattern shows that the highest peak of Mn0.25Fe2.75O4/Ag /Ag is located at 35.4° for Mn0.25Fe2.75O4/Ag which is spinel inverse cubic and 38.1° for face-centred cubic Ag. Based on the analysis, the crystal size of Mn0.25Fe2.75O4/Ag was 9.52 nm for Mn0.25Fe2.75O4/Ag and 12.5 nm for Ag. Ferrofluid fillers are composed of spherical particles and tend to agglomerate. The main functional groups of ferrofluid Mn0.25Fe2.75O4/Ag/Ag/OA/DMSO detected were C-O, S-O, Fe-O and Mn-O at wavenumbers 1170, 950, 707, 410 cm−1. The C-O and S-O functional groups are characteristic of OA, DMSO, and sunflower seed oil. The result is if the ferrofluid Mn0.25Fe2.75O4/Ag/OA/DMSO was successfully synthesized. The results of testing the antibacterial activity of ferrofluid using Escherichia coli bacteria and Staphylococcus aureus bacteria showed zone values of 5.72 mm and 6.60 mm. [ABSTRACT FROM AUTHOR]