1. Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis
- Author
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Jing LI, Su-sheng SONG, Yu-sheng ZHAO, Wei-wei GUO, Guang-hui GUO, Hui-ru PENG, Zhong-fu NI, Qi-xin SUN, and Ying-yin YAO
- Subjects
wheat ,Ta14-3-3 ,heterosis ,gene expression ,Agriculture (General) ,S1-972 - Abstract
14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.
- Published
- 2013
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