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Ganodermamushrooms have been widely used as functional food in China, Japan, and Korea. Ganodermatriterpenoids are deemed to be the main functional constituents. The structures of Ganodermatriterpenoids are complex but quite similar, which makes their analyses markedly limited. In this study, we developed a general 2D NMR method to differentiate Ganodermatriterpenoids, which classifies them into six types (A–F). Then, by the NMR-based isolation of A–F type triterpenoids from the fruiting bodies of G. resinaceum, four new compounds (1–4) and eight known compounds (5–12) were obtained. Moreover, combined with spiking experiments in 1D and 2D NMR spectra, compounds 5, 7, and 8, which belong to triterpenoids of A and B types, were identified. At the end, to achieve a more extensive application for this NMR method, a qNMR method for the absolute quantification of 5, 7, and 8in the gross triterpenoids from G. resinaceumwas set up. The results showed that this NMR method is reliable for the NMR-guided isolation and quantification of triterpenoids in G. resinaceum.