En este trabajo se presenta un estudio paleolimnológico basado en el análisis de múltiples indicadores en múltiples sitios con el objetivo de reconstruir la historia evolutiva de cinco lagos someros de la llanura Pampeana (Argentina) desde el Holoceno medio, y así realizar inferencias paleoclimáticas para esta región. Los cinco lagos estudiados respondieron sincrónicamente y mostraron el mismo patrón de evolución paleoambiental durante el Holoceno medio y tardío. Entre los ca. 7.000 y 700-500 años cal AP, se evidencia una primera fase clara, dominada por carofitas del género Chara, que cambia a los ca. 700-500 años cal AP hacia una fase turbia dominada por fitoplancton y con una importante presencia de carofitas sumergidas del grupo de las angiospermas, que indican fases claras que alternan con el estado turbio general. En la vegetación circundante, se evidencia un cambio desde una comunidad halófita que indica condiciones salobres e inestables hacia la comunidad de macrófitas que caracterizan en la actualidad a estos lagos, por lo cual sugiere ambientes con mayor estabilidad. Durante el Holoceno medio y por largos períodos (alrededor de 6.500 años) la estabilidad del paisaje se mantuvo debido a las condiciones climáticas más secas que las actuales, en las que predominaron fluctuaciones de sequías e inundaciones y/o condiciones de alta evaporación. Estas fluctuaciones son las que mantuvieron a las comunidades tanto acuáticas como terrestres. A partir de ca. 2.000 años cal AP el incremento de la diversidad de macrófitas sumergidas (carofitas y angiospermas) indica mayor aporte de nutrientes y materia orgánica, causado por un aumento en la intensidad y/o duración de las precipitaciones. A partir de este momento comienza un cambio gradual que se efectiviza a los ca. 700-500 años cal AP, con el establecimiento de las comunidades de macrófitas emergentes, flotantes y sumergidas, características de los sistemas actuales. High-resolution paleolimnological studies based on multi-proxy analysis constitute an important tool to reconstruct the evolution of aquatic systems as well as evaluating their responses to natural and/or anthropogenic forcing factors (Lotter, 2003; Birks and Birks, 2006). While individual palaeoecological studies reveal local developments, general patterns often only emerge when information from several sites is combined together. Numerous shallow lakes occur throughout the Pampa plain of Argentina, between 33° to 39°S and 57° to 66W. These lakes present two alternative states of equilibrium (Scheffer and Jeppesen, 2007; Scheffer and van Ness, 2007). Some of them are turbid lakes due to the high amount of algae, while others are clear macrophytedominated lakes. A third type of lake that can be recognized within the region is inorganic-turbid lakes, in which turbidity is caused by high amount of suspended inorganic material (Quirós et al., 2002; Allende et al., 2009). These lakes are characterized by low productivity, with scarce phytoplankton and macrophytes. Most of the lakes developed on deflation basins that originated during the late Pleistocene by the prevailing westerly winds. Often, the lakes are associated to lunettes, fixed palaeodunes that developed at the shore, on the windward side of the basins (Tricart, 1973; Zárate and Tripaldi, 2012). During the Holocene, the basins became areas of groundwater discharge and surface water accumulation gradually filling with sediments. Today, the lakes are nutrient-rich, eutrophic to hypereutrophic, and polymictic, too shallow to develop thermal stratification. Water depth and salinity are highly variable. During summer and/or during episodic droughts the lakes suffer significant reduction in water volume, and thus strong fluctuations in their water levels (Quirós and Drago, 1999; Sosnovsky and Quirós, 2006). These aquatic systems are able to support an abundance of macrophytes and phytoplankton, which have proven to leave an exceptional fossil record of environmental changes (Stutz et al. 2002, 2006, 2010, 2012; Fontana, 2005). For more than a decade the authors have been investigating these aquatic systems in order to reconstruct their evolutionary history and the regional environments with the main goal of inferring the past climatic conditions. In order to achieve this, multi-proxy analysis of diverse biological indicators were carried out in several lakes of the south-east region of the Pampa plain. Pollen, non-pollen palynomorphs and plant macrofossil remains and associated fauna were analysed in sediment sequences from lake Hinojales- San Leoncio (37º23'S; 57º23'W) and lake Tobares (37°30'S; 57°28'W). These new results were then combined with former investigations from lakes Lonkoy (37º12'S; 57º25'W), Nahuel Rucá (37º37'S; 57º26'W) and Hinojales (37º34'S; 57º27'W) (Fig. 1). The studied lakes range in suface from 200 to 300 ha and a water depth of about 1 m. The regional vegetation is characterized by temperate subhumid grasslands, knowns as pampas. The modern landscape is strongly influenced by human activities since the establishment of European settlements in the XVI century. The land has been intensively used, in particular for grassing, as well as for agriculture. Native trees are absent in the vegetation, except for Celtis ehrenbergiana, a deciduous tree known as tala, which occurs on Pleistocene lunette dunes and Holocene ridges of shell debris. The aquatic plant communities of the studied sites are characterized by several species of emergent, free-floating leaved and submerged macrophytes. The lakes are surrounded by a ring of Schoenoplectus californicus, among which other emergent macrophytes occur: e.g. Zizaniopsis bonariensis, Typha latifolia, Hydrocotyle bonariensis, H. ranunculoides, Alternanthera philloxeroides, Solanum glaucophyllum, Bacopa monnieri, Polygonum punctatum, Ranunculus apiifolius, Triglochin striata, and Phyla canescens. Near the shore, in sheltered areas, free floating plants like Ricciocarpus natans, Azolla filiculoides, Limnobium laevigatum, Lemna valdiviana, Wolffia brasiliensis, Wolffiella lingulata and W. oblonga form a dense carpet. Diverse submerged macrophytes occur when the water tends to be clear, so the light available is enough for their development: e.g. Myriophyllum elatinoides, Ceratophyllum demersum, Potamogeton pectinatus, and Zannichellia palustris together with diverse Charophytes. The studied sediment records were recovered with different samplers: Dacknovsky (Hinojales), vibracorer (Nahuel Rucá, Hinojales-SL and Tobares) and Livingston-type corer (Lonkoy). The chronology of the records is based on AMS radiocarbon age determinations on terrestrial plant remains, where possible (Table 1). Gastropod shells of Heleobia parchappii have also been used for dating, since previous studies have yielded reliable results (Fontana, 2005, 2007). Age-depth models (Fig. 2) are constructed using CLAM 2.2 (Blaauw, 2010) with the Southern Hemisphere calibration curve, SHCal13 (Hogg et al., 2013). The studied records, spanning the time since the middle Holocene, document similar evolutionary pattern. Changes in the different proxies are consistent, showing similar trends in the evolution of the aquatic ecosystems (Figs. 3-7). At the beginning of the records a clear phase characterized the water bodies, dominated by the green algae Chara, the dinoflagellate Peridinium and aquatic plants such as Myriophyllum, Ruppia and/or Potamogeton. At ca. 2,000 cal yr BP the diversity of submerged macrophyte communities increased, indicating clear water lakes with increasing nutrient content. Towards ca. 700-500 cal yr BP the lakes switched to a turbid phase dominated by phytoplankton together with water fleas and flatworms. Among the phytoplankton the main constitutes are: Pediastrum, Scenedesmus and Tetraedron species of green algae Desmidiaceae and the cyanobacterium Gloeotrichia. On land, halophyte plant communities, dominated by Chenopodiaceae, surrounded the water bodies during the first clear-water phase, suggesting instable periods with brackish conditions. Towards the present, the surroundings of the lakes are characterized by dominance of Cyperaceae together with Bacopa, Ranunculus, Polygonum, Typha and Apiaceae. This vegetation is characteristic of more stable environmental conditions. During the middle Holocene and part of the late Holocene, brackish-shallow lakes with clear water phases characterised the landscape of the south-east pampean plain. After ca. 2,000 cal. yr BP, water run-off and/or wind action increased, incorporating organic matter from the surroundings into the basins and initiating a gradual change towards the next phase. This dynamics could be associated to periods of pronounced drought followed by floods. A marked seasonality in the annual precipitation regimen would explain this dynamics indicating a regionally instable environment with dryer climatic conditions than present (Zárate et al., 1998; Zárate, 2005; Vilanova et al., 2010; Laprida et al., 2014). The change to turbid conditions at around 700-500 cal yr BP was probably caused by a significant input of nutrients into the basin, impeding the development of submerged aquatic plants, characteristic of the clear phase, which in turn favoured the expansion of phytoplankton. However, the presence of submerged macrophytes that tolerate some degree of turbidity like Miryophyllum, Ceratophyllum and Potamogeton would suggest that during some periods the level of turbidity did not reach critical values. The presence of submerged flowering plants may also suggest alternate periods of clear phases within a general turbid state. The synchronous change to a turbid face in all studied sites suggests a climatic regional trigger like an increase of precipitation, with a more stable seasonal regime. Similar values of precipitation compared to today did not occur until historical times (Irurzun et al., 2014; Laprida et al., 2014). This study constitutes the first of its type basing the reconstruction of paleoenvironmental and paleoclimatic conditions on the dynamics and functioning of the shallow lakes, studied in the context of multiproxy and multi-site analysis. Fil: Stutz, Silvina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Tonello, Marcela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Gonzalez Sagrario, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Navarro, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Fontana, Sonia Leonor. Universität Göttingen; Alemania