28 results on '"Stuhr S"'
Search Results
2. Structure and Chemical Organization in Damselfly Calopteryx haemorrhoidalis Wings: A Spatially Resolved FTIR and XRF Analysis with Synchrotron Radiation
- Author
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Stuhr, S, Vi, KT, Vongsvivut, J, Senkbeil, T, Yang, Y, Al Kobaisi, M, Baulin, VA, Werner, M, Rubanov, S, Tobin, MJ, Cloetens, P, Rosenhahn, A, Lamb, RN, Luque, P, Marchant, R, Ivanova, EP, Stuhr, S, Vi, KT, Vongsvivut, J, Senkbeil, T, Yang, Y, Al Kobaisi, M, Baulin, VA, Werner, M, Rubanov, S, Tobin, MJ, Cloetens, P, Rosenhahn, A, Lamb, RN, Luque, P, Marchant, R, and Ivanova, EP
- Abstract
Insects represent the majority of known animal species and exploit a variety of fascinating nanotechnological concepts. We investigated the wings of the damselfly Calopteryx haemorrhoidalis, whose males have dark pigmented wings and females have slightly pigmented wings. We used scanning electron microscopy (SEM) and nanoscale synchrotron X-ray fluorescence (XRF) microscopy analysis for characterizing the nanostructure and the elemental distribution of the wings, respectively. The spatially resolved distribution of the organic constituents was examined by synchrotron Fourier transform infrared (s-FTIR) microspectroscopy and subsequently analyzed using hierarchical cluster analysis. The chemical distribution across the wing was rather uniform with no evidence of melanin in female wings, but with a high content of melanin in male wings. Our data revealed a fiber-like structure of the hairs and confirmed the presence of voids close to its base connecting the hairs to the damselfly wings. Within these voids, all detected elements were found to be locally depleted. Structure and elemental contents varied between wing membranes, hairs and veins. The elemental distribution across the membrane was rather uniform, with higher Ca, Cu and Zn levels in the male damselfly wing membranes.
- Published
- 2018
3. Structure and Chemical Organization in Damselfly Calopteryx haemorrhoidalis Wings: A Spatially Resolved FTIR and XRF Analysis with Synchrotron Radiation
- Author
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Universitat Rovira i Virgili, Stuhr S, Truong VK, Vongsvivut J, Senkbeil T, Yang Y, Al Kobaisi M, Baulin VA, Werner M, Rubanov S, Tobin MJ, Cloetens P, Rosenhahn A, Lamb RN, Luque P, Marchant R, Ivanova EP, Universitat Rovira i Virgili, and Stuhr S, Truong VK, Vongsvivut J, Senkbeil T, Yang Y, Al Kobaisi M, Baulin VA, Werner M, Rubanov S, Tobin MJ, Cloetens P, Rosenhahn A, Lamb RN, Luque P, Marchant R, Ivanova EP
- Abstract
Insects represent the majority of known animal species and exploit a variety of fascinating nanotechnological concepts. We investigated the wings of the damselfly Calopteryx haemorrhoidalis, whose males have dark pigmented wings and females have slightly pigmented wings. We used scanning electron microscopy (SEM) and nanoscale synchrotron X-ray fluorescence (XRF) microscopy analysis for characterizing the nanostructure and the elemental distribution of the wings, respectively. The spatially resolved distribution of the organic constituents was examined by synchrotron Fourier transform infrared (s-FTIR) microspectroscopy and subsequently analyzed using hierarchical cluster analysis. The chemical distribution across the wing was rather uniform with no evidence of melanin in female wings, but with a high content of melanin in male wings. Our data revealed a fiber-like structure of the hairs and confirmed the presence of voids close to its base connecting the hairs to the damselfly wings. Within these voids, all detected elements were found to be locally depleted. Structure and elemental contents varied between wing membranes, hairs and veins. The elemental distribution across the membrane was rather uniform, with higher Ca, Cu and Zn levels in the male damselfly wing membranes.
- Published
- 2018
4. Morphological analysis of cerium oxide stabilized nanoporous gold catalysts by soft X-ray ASAXS
- Author
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Rumancev, C., primary, von Gundlach, A. R., additional, Baier, S., additional, Wittstock, A., additional, Shi, J., additional, Benzi, F., additional, Senkbeil, T., additional, Stuhr, S., additional, Garamusx, V. M., additional, Grunwaldt, J.-D., additional, and Rosenhahn, A., additional
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- 2017
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5. Effects of developmental care on behavioral organization in very-low-birth-weight infants.
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Becker PT, Grunwald PC, Moorman J, and Stuhr S
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- 1993
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6. Outcomes of developmentally supportive nursing care for very low birth weight infants.
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Becker PT, Grunwald PC, Moorman J, and Stuhr S
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- 1991
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7. Bemanding af fornyelsesprojekter gennem frivillig tilmelding
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Pernille Eskerod, Anna Lund Jepsen, and Stuhr, S.
8. Clinical and radiographic changes following transcrestal sinus augmentation: A scoping review of the last 25 years.
- Author
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Decker AM, Stuhr S, Testori T, and Wang HL
- Subjects
- Humans, Dental Implantation, Endosseous methods, Dental Implants, Sinus Floor Augmentation methods
- Abstract
Introduction: Maxillary sinus floor elevation is a surgical procedure intended to increase the volume of the bone vertically to accommodate dental implant placement. This intervention is frequently required for implant installation in the posterior maxilla, where the bone may be insufficient for securing implants of necessary length and stability. Sinus floor elevation can be completed either through a direct approach with a "window" through the lateral wall of the alveolar ridge or an indirect "transcrestal/transalveolar" sinus floor elevation (TSFE), which accesses the sinus floor through the crest of the edentulous ridge. Our study aims to provide a comprehensive scoping review of research conducted over the past 25 years on TSFE, specifically., Methods: A literature search aimed at identifying pertinent literature for the purpose of this PRISMA-ScR-compliant scoping review was conducted. Only randomized controlled trials, non-randomized controlled trials, prospective cohort studies, and case series that met the eligibility criteria were selected. Relevant data from these studies were extracted. Primary outcome measures included radiographic bone levels and implant failure >5 years. Secondary outcome measures included implant stability at time of placement and complications. Interventions reported in the selected studies were grouped based on treatment modality, which were then compared with the control therapy (traditional osteotome technique) after a minimum of 12 months healing period., Results: Our search yielded 633 records, and after deduplication, 574 of these were screened. Application of the eligibility criteria led to the inclusion of 37 articles in the final selection. Case selection for included studies enrolling subjects: Four different transcrestal sinus elevation treatment modalities were identified: (a) osteotome, (b) piezoelectric surgery, (c) osseodensification, and (d) hydraulic techniques. Due to the heterogeneity of the studies, no superior approach for TSFE could be identified. Overall, all techniques demonstrated high implant survival rates., Conclusion: Comprehensive understanding of the patient's medical history, available armamentarium, and post-operative complications/management strategies are all essential to the completion of a successful TSFE approach for implant placement in the posterior maxilla, regardless of the treatment modality used., (© 2024 The Author(s). Clinical Implant Dentistry and Related Research published by Wiley Periodicals LLC.)
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- 2024
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9. Autonomous Dental Implant Robotic System Utilization for Implant Placement and Transcrestal Sinus Elevation Using Osseodensification: A Case Report.
- Author
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Sun M, Yue C, Stuhr S, Fu X, and Wang HL
- Subjects
- Humans, Dental Implants, Dental Prosthesis, Implant-Supported, Maxilla surgery, Robotic Surgical Procedures methods, Robotics, Dental Implantation, Endosseous methods, Sinus Floor Augmentation methods
- Abstract
Robotic systems have revolutionized various industries, and dentistry is no exception. Recently, due to the robust advancements in artificial intelligence and technology, there has been a significant evolution of dental robotic systems, ranging from surgeon-controlled and robot-assisted operations to more autonomous processes. The present clinical case report describes a 1-year follow-up of the successful use of an autonomous dental implant robot system with an osseodensification protocol for implant osteotomy preparation, maxillary sinus elevation, and simultaneous implant placement at the maxillary second premolar site. A prefabricated provisional prosthesis was delivered immediately after implant placement, with final prosthesis delivery at 3 months. The findings from this report demonstrate the integration and clinical augmentation of more autonomous protocols in the field of implant dentistry using dental robots.
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- 2024
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10. Effect of suturing in root coverage via coronally advanced flaps: A systematic review.
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Ariceta A, Chambrone L, Stuhr S, and Couso-Queiruga E
- Abstract
Background: To analyze the evidence about the influence of the suturing technique and material in terms of the percentage of mean root coverage (%MRC) following root coverage therapy in teeth diagnosed with single/localized gingival recession defects (GRD) via a monolaminar coronally advanced flap., Methods: The protocol of this systematic review was registered in PROSPERO (CRD42024514043). A literature search was conducted to identify investigations that fulfilled the eligibility criteria. Variables of interest were extracted, subsequently categorized, and qualitatively analyzed., Results: A total of 15 randomized clinical trials, including 301 localized GRD in non-molar sites classified as Miller class I-II/RT1, in 253 patients were included. The studies reporting the combination of sling and single interrupted sutures, or interrupted sutures alone showed an MRC of 70.2%±16.6%, and 74.1%±0.75%, respectively. The highest MRC was observed in the studies using polyglactin 910 with a pooled value of 76.6% ± 15.3%, and monofilament materials, with a pooled MRC of 74.8%±7.1%. When the suturing diameter was evaluated, the highest pooled MRC with values of 79.1%±9.8% was observed with the use of 5-0., Conclusions: For the treatment of single/localized GRD in non-molar sites via a monolaminar coronally advanced flap, the use of a combination of sling and single interrupted sutures, or single interrupted sutures, polyglactin 910 or monofilament materials, and material diameter of 5-0 showed a higher MRC as compared to the use of expanded polytetrafluoroethylene, and silk with/without dressing, and other suture diameters., Key Points: There were no differences in the percentage of root coverage achieved between the use of sling and single interrupted, versus single interrupted sutures alone on the treatment of single/localized GRD in non-molar sites. Polyglactin 910 and monofilament sutures resulted in a higher percentage of root coverage achieved as compared to expanded polytetrafluoroethylene, and silk with/without dressing. The use of 5-0 material diameter showed the highest percentage of root coverage achieved., Plain Language Summary: This study was primarily aimed at evaluating how different suturing techniques and materials could affect the percentage of root coverage in single/localized recession defects, without the use of soft tissue substitutes or autogenous soft tissue grafts. After the pooled analyses of 15 randomized clinical trials that fulfilled the inclusion criteria, we observed that the adequate selection of suturing techniques, materials, and size could lead to a higher percentage of root coverage. Specifically, the use of single interrupted with or without sling sutures at the most coronal portion, Polyglactin 910 or monofilament materials, and size of 5-0 showed a higher percentage of root coverage as compared to the use of expanded polytetrafluoroethylene, and silk with/without dressing, and other suture diameters., (© 2024 The Author(s). Clinical Advances in Periodontics published by Wiley Periodicals LLC on behalf of American Academy of Periodontology.)
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- 2024
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11. Publication Delay of Systematic Reviews in Dentistry: Findings, Implications, and Potential Solutions.
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Brown E, Stuhr S, Chambrone L, Childs CA, Avila-Ortiz G, and Elangovan S
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- Humans, Time Factors, Review Literature as Topic, Systematic Reviews as Topic, Dental Research, Publishing, Dentistry
- Abstract
Clinicians, researchers, and policymakers often rely on the available scientific evidence to make strategic decisions. Systematic reviews (SRs) occupy an influential position in the hierarchy of scientific evidence. The findings of wellconducted SRs may provide valuable information to answer specific research questions1,2 and identify existing gaps for future research.3 Therefore, it is of supreme importance that SRs are published promptly, reducing as much as possible the time elapsed between the last date of the search for primary studies and the actual publication date. A study published in 2014 assessed the publication delay of SRs in orthodontics, revealing that the median time interval from the last search to publication was more than 1 year (13.2 months).4 Delays in the publication of SRs or original research articles may depend on author-related factors (eg, timing of resubmission after receiving feedback from reviewers) or journal-related factors (eg, time taken to process a submission).5-7 Regardless of the reasons, clinical recommendations and translation of SR findings may be affected by publication delay. We assessed the extent of publication delay of systematic reviews in dentistry with the purpose of addressing its implications and presenting potential solutions.
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- 2024
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12. Tooth autotransplantation outcomes using platelet-rich fibrin: A promising approach; case study.
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SamavatiJame F, Alrmali A, Galindo-Fernandez P, Stuhr S, and Wang HL
- Abstract
Background: Autogenous tooth transplantation refers to a surgical procedure involving the relocation of a tooth within the same individual. Incorporating platelet-rich fibrin (PRF) in this procedure holds the potential to improve healing, accelerate recovery, and optimize treatment outcomes., Methods: In this article, the authors illustrate a PRF-based approach for autogenous tooth transplantation through two case scenarios. These cases outline the surgical steps of tooth transplantation and demonstrate the potential role of PRF in enhancing soft tissue healing. Furthermore, the article provides insights from a long-term follow-up spanning over 7 years., Results: Tooth transplantation in young adults is promising but depends on factors such as root development stage and donor tooth size matching. Including PRF may improve healing, at least in the short term, due to its rich concentration of growth factors and cytokines, promoting effective tissue regeneration., Conclusions: Autogenous tooth transplantation has shown to be a viable treatment option for replacing the missing dentition. Adding PRF to the autogenous tooth transplantation procedure may speed up and enhance the treatment outcome. While the favorable results of these cases might be partially attributed to the use of PRF, the contribution of PRF to the healing process of tooth transplant remains conjectural and requires validation through additional research., Key Points/highlights: Tooth autotransplantation can be performed in younger patients without requiring root canal treatment, while also potentially benefiting from the incorporation of platelet-rich fibrin (PRF)., (© 2024 The Authors. Clinical Advances in Periodontics published by Wiley Periodicals LLC on behalf of American Academy of Periodontology.)
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- 2024
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13. A decision-making tree for evaluating an esthetically compromised single dental implant.
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Alrmali A, Stuhr S, Saleh MHA, Latimer J, Kan J, Tarnow DP, and Wang HL
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- Esthetics, Dental, Dental Implantation, Endosseous methods, Connective Tissue transplantation, Dental Implants, Dental Implants, Single-Tooth
- Abstract
Objective: To develop a comprehensive decision-making tree for evaluating mid-facial peri-implant soft tissue dehiscence in the esthetic zone and provide a systematic approach for assessing various clinical case scenarios, determining appropriate treatment strategies, and considering factors such as the need for soft tissue augmentation, prosthetic changes, or implant removal., Clinical Considerations: This clinical decision tree illustrates numerous case scenarios with various esthetic complications around an esthetically compromised, but clinically healthy single implant and provides clinicians with possible solutions as a predictable map for horizontal and vertical soft tissue augmentation in order to manage different clinical circumstances. According to current evidence, the key to treating such esthetic complications is the use of an adequate pre-surgical prosthetic interdisciplinary approach with proper surgical techniques in order to optimize soft tissue dimensions and create better esthetic results. This may be accomplished through a purely surgical, combination of surgical and prosthetic, or purely prosthetic approaches., Conclusions: The present report describes a series of successfully treated peri-implant esthetic complication cases in accordance with the decision-making tree that the authors recommend in order to achieve better long-term esthetic outcomes., Clinical Significance: The combination of adequate pre-surgical prosthetic interdisciplinary collaboration and proper surgical technique is critical in the optimization of sufficient soft tissue dimensions and contributes to a more highly esthetic result. This study demonstrates a clinical decision-making tree to provide comprehensive, effective therapy of an esthetically compromised dental implant by using one of the following approaches: purely prosthetic, purely surgical, or a combination of surgical and prosthetic with or without abutment removal., (© 2023 The Authors. Journal of Esthetic and Restorative Dentistry published by Wiley Periodicals LLC.)
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- 2023
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14. Histological assessment and gene expression analysis of intra-oral soft tissue graft donor sites.
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Stuhr S, Nör F, Gayar K, Couso-Queiruga E, Chambrone L, Gamborena I, Kumar P, Avila-Ortiz G, and Ganesan SM
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- Humans, Collagen, Mucous Membrane, Gene Expression Profiling, Connective Tissue transplantation, Palate anatomy & histology
- Abstract
Aim: To determine the structural and gene expression features of different intra-oral soft tissue donor sites (i.e., anterior palate, posterior palate, maxillary tuberosity and retromolar pad)., Materials and Methods: Standardized mucosal tissue punch biopsies were collected from at least one donor site per subject. Histological processing was performed to determine tissue morphometry and quantify collagen composition. Site-specific gene distribution was mapped using targeted gene expression analysis and validated using real time polymerase chain reaction (qPCR)., Results: A total of 50 samples from 37 subjects were harvested. Epithelial thickness did not differ between sites. However, lamina propria was thicker in the maxillary tuberosity (2.55 ± 0.92 mm) and retromolar pad (1.98 ± 0.71 mm) than in the lateral palate. Type I collagen was the predominant structural protein in the lamina propria (75.06%-80.21%). Genes involving collagen maturation and extracellular matrix regulation were highly expressed in the maxillary tuberosity and retromolar pad, while lipogenesis-associated genes were markedly expressed in the lateral palate. The retromolar pad showed the most distinct gene expression profile, and the anterior and posterior palate displayed similar transcription profiles., Conclusions: Tissue samples harvested from the anterior and posterior palate differed morphologically from those from the maxillary tuberosity and retromolar pad. Each intra-oral site showed a unique gene expression profile, which might impact their biological behaviour and outcomes of soft tissue augmentation procedures., (© 2023 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.)
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- 2023
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15. 3D vertical soft tissue augmentation of aesthetically compromised permanent submerged dental implants.
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Alrmali AE, Saleh MHA, Gnao EH, Stuhr S, Rasperini G, and Wang HL
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Case Presentation: Midfacial peri-implant soft tissue recession poses a significant challenge to achieving satisfactory aesthetic outcomes and requires a comprehensive, multidisciplinary approach. The present study describes two challenging cases in which implants were aesthetically compromised. A predictable technique was employed to manage these cases by using the implant itself as a tent to achieve 3D horizontal and vertical soft tissue building, which resulted in improved patient satisfaction. The first case involved a deep, severely buccally placed implant situated adjacent to compromised tooth structure. Horizontal and vertical soft tissue augmentation were carried out using a healing abutment to maintain the connective tissue coronal to the implant. The final fixed prosthesis was then delivered on top of the permanent submerged implant, with excellent soft tissue outcomes and a high level of patient satisfaction. The second case involved two compromised, deep, buccally placed implants that were managed by performing a permanent implant submergence technique on the maxillary left central incisor implant and augmenting the soft tissue vertically and horizontally. The final fixed prosthesis was delivered between the maxillary left lateral incisor implant and the previously prepared maxillary right central incisor, resulting in an acceptable aesthetic outcome. The technique presented managed the peri-implant soft tissue dehiscence effectively and restored the previously deficient peri-implant papillae., Conclusions: The key to treating challenging aesthetic complications encountered with implants is presurgical prosthetic preparation followed by use of a comprehensive surgical technique to optimise soft tissue thickness and height and address compromised aesthetics in a single surgical step. Use of a permanent implant submergence technique with remediation of associated defects may be a viable clinical approach that is not often explored for these types of defects.
- Published
- 2023
16. Decision Making in the Restoration of Endodontically Treated Teeth: Effect of Biomimetic Dentistry Training.
- Author
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Kimble P, Stuhr S, McDonald N, Venugopalan A, Campos MS, and Cavalcanti B
- Abstract
The restoration of endodontically treated teeth (ETT) is challenging as these teeth often present with structural deficiencies. Currently, there is no consensus regarding the final restoration choice. Historically, the full coverage crown was the universally selected treatment for endodontically treated teeth. With advances in adhesive and biomimetic dentistry, more minimally invasive treatment modalities have become a viable option. With this study, we aim to understand the restorative decision of the general dentist with or without additional training in biomimetic dentistry. Seventy-eight general dentists, with or without biomimetic training, were surveyed to determine their restorative preferences on five extracted posterior teeth, categorized according to volumetric loss of tooth structure, as indicated by the number of missing walls, the isthmus width, the presence or absence of marginal ridges, and cusps. CAD/CAM reconstructions were made with the teeth to analyze the volume of tooth loss and compare these with the survey results. Data were compared using the chi-squared test and Fisher's exact test. The frequency of responses recommending a crown and the volume of tooth loss were correlated using the Pearson test ( p < 0.05). For all five teeth, survey responses showed a statistically significant difference in the restorative decision of full coverage versus alternative restorations, with biomimetic dentists selecting a direct restoration or inlay/onlay in lieu of a full coverage crown ( n = 63, p < 0.05). The age of the participant did not have a significant impact on the restorative decision making process for these teeth. The biomimetic trained dentists showed a greater tendency to select a crown option only when the volume of tooth loss was greatest, otherwise their restorative decisions tended towards the conservative treatment options. This study also demonstrates a novel method of digitally developing a volume of tooth loss to compare against the visual interpretation of the volume of tooth loss.
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- 2023
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17. Comparative histological evaluation of intra- and extraorally de-epithelialized connective tissue graft samples harvested from the posterior palate region.
- Author
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Couso-Queiruga E, Gonzalez-Martin O, Stuhr S, Gamborena I, Chambrone L, and Avila-Ortiz G
- Subjects
- Humans, Connective Tissue transplantation, Palate surgery, Treatment Outcome, Gingival Recession surgery
- Abstract
Background: Autologous connective tissue graft (CTG) is generally considered the gold standard for peri-implant soft tissue phenotype modification and root coverage therapy. The presence of epithelial remnants in CTG has been associated with complications after soft tissue augmentation surgery. However, a specific method for de-epithelization that is patently superior has not been identified yet. This study aimed to evaluate the effectiveness of two different approaches to de-epithelialize CTG samples harvested from the posterior palate., Methods: Patients in need of periodontal or implant-related surgery that required harvesting a CTG from the posterior palate region were recruited. CTG samples harvested with an indirect approach were de-epithelialized using either an intraoral (IO group) or an extraoral (EO group) method. Tissue specimens were subsequently processed for histological analysis. The presence or absence of oral epithelial remnants was determined by two examiners using light microscopy., Results: Twenty-four patients, who provided a total of 46 analyzable CTG samples, were enrolled in this study. Histological assessment revealed that 19 out of 22 samples in the IO group were free of epithelial remnants. In the EO group, 20 out of 24 samples did not exhibit epithelial residues. These results translate into 86.4% and 83.3% of epithelium-free samples in the IO and EO groups, respectively., Conclusions: Although the intraoral method may provide several practical advantages compared with the extraoral technique, both approaches for de-epithelialization of CTG samples harvested from the posterior palate region tested in this study were comparably effective. However, none of these methods ensured complete removal of the oral epithelium in a predictable manner, which should be considered in clinical practice., (© 2023 American Academy of Periodontology.)
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- 2023
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18. Waistline to the gumline: Relationship between obesity and periodontal disease-biological and management considerations.
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Ganesan SM, Vazana S, and Stuhr S
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- Humans, Obesity complications, Obesity epidemiology, Oral Health, Risk Factors, Periodontal Diseases complications, Periodontal Diseases epidemiology, Periodontal Diseases therapy, Periodontitis
- Abstract
Obesity is a pandemic and periodontitis is the sixth most prevalent disease in the world. These two noncommunicable diseases share several risk determinants. Epidemiologic evidence from the last 2 decades has established an increase in periodontitis prevalence in obese and overweight individuals. Biologic mechanisms potentially linking obesity and periodontal disease are adiposity-associated hyperinflammation, microbial dysbiosis, altered immune response, specific genetic polymorphisms, and increased stress. However, because of the lack of longitudinal interventional studies and randomized clinical trials, there is insufficient evidence to determine the cause-effect relationship between these two diseases. Despite this, the negative impact of obesity on oral health is well established. Several logistic and physiologic complications are associated with treating obese patients in a dental setting, and it requires an interprofessional team approach. Oral health care professionals need to be aware of the specific management considerations while rendering for this cohort, including modified practice facility and equipment, tailored supportive periodontal therapy, and heightened precaution during conscious sedation and surgical procedures., (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2021
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19. Evaluation of poly lactic-co-glycolic acid-coated β-tricalcium phosphate for alveolar ridge preservation: A multicenter randomized controlled trial.
- Author
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Saito H, Couso-Queiruga E, Shiau HJ, Stuhr S, Prasad H, Allareddy TV, Reynolds MA, and Avila-Ortiz G
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- Alveolar Process diagnostic imaging, Alveolar Process surgery, Bone Transplantation, Calcium Phosphates, Glycolates, Glycols, Humans, Tooth Extraction, Tooth Socket diagnostic imaging, Tooth Socket surgery, Alveolar Bone Loss diagnostic imaging, Alveolar Bone Loss prevention & control, Alveolar Bone Loss surgery, Alveolar Ridge Augmentation
- Abstract
Background: Alveolar ridge preservation via socket grafting (ARP-SG) is indicated to attenuate physiologic alveolar bone resorption as a consequence of tooth extraction. However, a specific bone grafting material that is patently superior has not been identified yet. The aim of this randomized controlled trial was to evaluate the efficacy of a moldable alloplastic graft, Poly Lactic-Co-Glycolic Acid-Coated β-Tricalcium Phosphate (PLGA-β-TCP), for ARP purposes [Group A] compared to freeze-dried bone allograft (FDBA) particles covered with a rapidly absorbable collagen dressing (RACD) (Group B) in function of a panel of radiographic, histomorphometric, and implant-related outcomes., Methods: Patients in need of extraction of a single posterior tooth (premolar or molar) and subsequent replacement with a dental implant were recruited and randomly allocated into one of the two treatment groups. Follow-up visits took place at 1, 2, 4, 8, and 16 weeks. Cone-beam Computed Tomography (CBCT) scans were obtained at baseline and at 16 weeks. Sites were re-entered at 20 weeks for bone core biopsy harvesting and subsequent implant placement. After delivery of the final implant-supported restoration, follow-up visits were scheduled at 6 and 12 months to monitor peri-implant tissue health and marginal bone levels using standardized intraoral periapical radiographs., Results: A total of 45 patients were initially enrolled in the study, of whom 43 received an implant and 32 completed the study. Healing was uneventful in all sites after ARP-SG and implant placement. No site required bone augmentation to allow for implant placement. CBCT scan analyses showed no statistically significant differences between groups in terms of reduction of horizontal width, midbuccal / midlingual height and ridge volume. Histomorphometric assessments revealed a statistically significant difference between both groups in terms of mineralized tissue formation (Group A = 27.0% ± 22.1% versus Group B = 38.2% ± 12.5%; P < 0.05). On the contrary, no significant differences were observed regarding percent of remaining bone grafting material and non-mineralized tissue. No implant failed throughout the study period and marginal bone level change was negligible in both groups., Conclusions: Although a higher proportion of mineralized tissue was associated with the use of FDBA+RACD compared to PLGA-β-TCP alone, both ARP-SG approaches rendered comparable outcomes in terms of maintenance of alveolar bone dimensions, feasibility of implant placement, implant survival, and peri-implant bone level stability up to 12 months post-loading., (© 2020 American Academy of Periodontology.)
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- 2021
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20. Micro x-ray fluorescence analysis of trace element distribution in frozen hydrated HeLa cells at the P06 beamline at Petra III.
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Rumancev C, Vöpel T, Stuhr S, von Gundlach A, Senkbeil T, Ebbinghaus S, Garrevoet J, Falkenberg G, De Samber B, Vincze L, Rosenhahn A, and Schroeder W
- Subjects
- HeLa Cells, Humans, Vacuum, X-Rays, Freezing, Spectrometry, X-Ray Emission, Trace Elements analysis, Water chemistry
- Abstract
X-ray fluorescence analysis enables the study of trace element distributions in biological specimens. When this analysis is done under cryogenic conditions, cells are cryofixed as closely as possible to their natural physiological state, and the corresponding intracellular elemental densities can be analyzed. Details about the experimental setup used for analysis at the P06 beamline at Petra III, DESY and the used cryo-transfer system are described in this work. The system was applied to analyze the elemental distribution in single HeLa cells, a cell line frequently used in a wide range of biological applications. Cells adhered to silicon nitride substrates were cryoprotected within an amorphous ice matrix. Using a continuous scanning scheme and a KB x-ray focus, the distribution of elements in the cells was studied. We were able to image the intracellular potassium and zinc levels in HeLa cells as two key elements relevant for the physiology of cells.
- Published
- 2021
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21. Post-extraction dimensional changes: A systematic review and meta-analysis.
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Couso-Queiruga E, Stuhr S, Tattan M, Chambrone L, and Avila-Ortiz G
- Subjects
- Alveolar Process, Humans, Tooth Extraction, Tooth Socket surgery, Alveolar Bone Loss diagnostic imaging, Alveolar Ridge Augmentation
- Abstract
Aim: To analyse the evidence pertaining to post-extraction dimensional changes in the alveolar ridge after unassisted socket healing., Materials and Methods: The protocol of this PRISMA-compliant systematic review (SRs) was registered in PROSPERO (CRD42020178857). A literature search to identify studies that fulfilled the eligibility criteria was conducted. Data of interest were extracted. Qualitative and random-effects meta-analyses were performed if at least two studies with comparable features and variables reported the same outcome of interest., Results: Twenty-eight articles were selected, of which 20 could be utilized for the conduction of quantitative analyses by method of assessment (i.e. clinical vs radiographic measurements) and location (i.e. non-molar vs molar sites). Pooled estimates revealed that mean horizontal, vertical mid-facial and mid-lingual ridge reduction assessed clinically in non-molar sites was 2.73 mm (95% CI: 2.36-3.11), 1.71 mm (95% CI: 1.30-2.12) and 1.44 mm (95% CI: 0.78-2.10), respectively. Mean horizontal, vertical mid-facial and mid-lingual ridge reduction assessed radiographically in non-molar sites was 2.54 mm (95% CI: 1.97-3.11), 1.65 mm (95% CI: 0.42-2.88) and 0.87 mm (95% CI: 0.36-1.38), respectively. Mean horizontal, vertical mid-facial and mid-lingual ridge reduction assessed radiographically in molar sites was 3.61 mm (95% CI: 3.24-3.98), 1.46 mm (95% CI: 0.73-2.20) and 1.20 mm (95% CI: 0.56-1.83), respectively., Conclusion: A variable amount of alveolar bone resorption occurs after unassisted socket healing depending on tooth type., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2021
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22. The impact of a modified electronic probe tip design on patient perception of discomfort during periodontal probing using standardized probing force: A randomized controlled trial.
- Author
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Harrison PL, Stuhr S, and Shaddox LM
- Subjects
- Adult, Humans, Pain Measurement, Perception, Periodontal Pocket, Electronics, Periodontics
- Abstract
Aims: This study evaluated pain associated with electronic probing comparing two commercially available probe tip designs using standardized force., Material and Methods: Twenty adult patients with slight-moderate chronic periodontitis received periodontal probing using controlled-force electronic probe at 2 visits. In visit 1, a random arch was probed with either a ball-end (0.6 mm diameter, 408 kPa; Test) or straight (0.45 mm diameter, 726 kPa; Control) probe tip. The opposing arch was then probed using the other probe tip. Discomfort associated with each probing episode was recorded using visual analogue scale (VAS). 7 days later, tip assignments were alternated from visit 1 and VAS re-scored. VAS scores were compared by probe tip, arches and visits., Results: Both tips provided similar VAS scores (median 13.5 for ball-end and 14 for straight, p = .3713). However, the straight tip was associated with decreased VAS scores in the maxilla (p = .01). Overall, VAS values did not differ by arch or study visit. Individual VAS scores showed high levels of correlation between study visits and between tips used (R
2 = .86 and 0.64, respectively, p < .0001)., Conclusions: Both probing approaches were associated with low levels of pain on probing. The straight tip may be perceived as more comfortable in the maxilla., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)- Published
- 2020
- Full Text
- View/download PDF
23. Soft X-ray diffraction patterns measured by a LiF detector with sub-micrometre resolution and an ultimate dynamic range.
- Author
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Makarov S, Pikuz S, Ryazantsev S, Pikuz T, Buzmakov A, Rose M, Lazarev S, Senkbeil T, von Gundlach A, Stuhr S, Rumancev C, Dzhigaev D, Skopintsev P, Zaluzhnyy I, Viefhaus J, Rosenhahn A, Kodama R, and Vartanyants IA
- Abstract
The unique diagnostic possibilities of X-ray diffraction, small X-ray scattering and phase-contrast imaging techniques applied with high-intensity coherent X-ray synchrotron and X-ray free-electron laser radiation can only be fully realized if a sufficient dynamic range and/or spatial resolution of the detector is available. In this work, it is demonstrated that the use of lithium fluoride (LiF) as a photoluminescence (PL) imaging detector allows measuring of an X-ray diffraction image with a dynamic range of ∼10
7 within the sub-micrometre spatial resolution. At the PETRA III facility, the diffraction pattern created behind a circular aperture with a diameter of 5 µm irradiated by a beam with a photon energy of 500 eV was recorded on a LiF crystal. In the diffraction pattern, the accumulated dose was varied from 1.7 × 105 J cm-3 in the central maximum to 2 × 10-2 J cm-3 in the 16th maximum of diffraction fringes. The period of the last fringe was measured with 0.8 µm width. The PL response of the LiF crystal being used as a detector on the irradiation dose of 500 eV photons was evaluated. For the particular model of laser-scanning confocal microscope Carl Zeiss LSM700, used for the readout of the PL signal, the calibration dependencies on the intensity of photopumping (excitation) radiation (λ = 488 nm) and the gain have been obtained.- Published
- 2020
- Full Text
- View/download PDF
24. Structure and Chemical Organization in Damselfly Calopteryx haemorrhoidalis Wings: A Spatially Resolved FTIR and XRF Analysis with Synchrotron Radiation.
- Author
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Stuhr S, Truong VK, Vongsvivut J, Senkbeil T, Yang Y, Al Kobaisi M, Baulin VA, Werner M, Rubanov S, Tobin MJ, Cloetens P, Rosenhahn A, Lamb RN, Luque P, Marchant R, and Ivanova EP
- Subjects
- Animals, Female, Male, Odonata anatomy & histology, Spectrometry, X-Ray Emission instrumentation, Spectroscopy, Fourier Transform Infrared instrumentation, Synchrotrons, Wings, Animal chemistry
- Abstract
Insects represent the majority of known animal species and exploit a variety of fascinating nanotechnological concepts. We investigated the wings of the damselfly Calopteryx haemorrhoidalis, whose males have dark pigmented wings and females have slightly pigmented wings. We used scanning electron microscopy (SEM) and nanoscale synchrotron X-ray fluorescence (XRF) microscopy analysis for characterizing the nanostructure and the elemental distribution of the wings, respectively. The spatially resolved distribution of the organic constituents was examined by synchrotron Fourier transform infrared (s-FTIR) microspectroscopy and subsequently analyzed using hierarchical cluster analysis. The chemical distribution across the wing was rather uniform with no evidence of melanin in female wings, but with a high content of melanin in male wings. Our data revealed a fiber-like structure of the hairs and confirmed the presence of voids close to its base connecting the hairs to the damselfly wings. Within these voids, all detected elements were found to be locally depleted. Structure and elemental contents varied between wing membranes, hairs and veins. The elemental distribution across the membrane was rather uniform, with higher Ca, Cu and Zn levels in the male damselfly wing membranes.
- Published
- 2018
- Full Text
- View/download PDF
25. Quantitative ptychographic bio-imaging in the water window.
- Author
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Rose M, Senkbeil T, von Gundlach AR, Stuhr S, Rumancev C, Dzhigaev D, Besedin I, Skopintsev P, Loetgering L, Viefhaus J, Rosenhahn A, and Vartanyants IA
- Subjects
- Photons, Radiation Dosage, Algorithms, Fibroblasts, Microscopy methods, Radiographic Image Enhancement methods, X-Ray Diffraction
- Abstract
Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·10
6 . As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·104 Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells.- Published
- 2018
- Full Text
- View/download PDF
26. Salt induced reduction of lysozyme adsorption at charged interfaces.
- Author
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Göhring H, Paulus M, Salmen P, Wirkert F, Kruse T, Degen P, Stuhr S, Rehage H, and Tolan M
- Subjects
- Adsorption drug effects, Animals, Dose-Response Relationship, Drug, Fatty Acids chemistry, Membranes, Artificial, Surface Properties, Muramidase chemistry, Sodium Chloride pharmacology
- Abstract
A study of lysozyme adsorption below a behenic acid membrane and at the solid-liquid interface between aqueous lysozyme solution and a silicon wafer in the presence of sodium chloride is presented. The salt concentration was varied between 1 mmol L(-1) and 1000 mmol L(-1). X-ray reflectivity data show a clear dependence of the protein adsorption on the salt concentration. Increasing salt concentrations result in a decreased protein adsorption at the interface until a complete suppression at high concentrations is reached. This effect can be attributed to a reduced attractive electrostatic interaction between the positively charged proteins and negatively charged surfaces by charge screening. The measurements at the solid-liquid interfaces show a transition from unoriented order of lysozyme in the adsorbed film to an oriented order with the short protein axis perpendicular to the solid-liquid interface with rising salt concentration.
- Published
- 2015
- Full Text
- View/download PDF
27. Barriers to colorectal cancer screening in Hispanics in the United States: an integrative review.
- Author
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Wang J, Moehring J, Stuhr S, and Krug M
- Subjects
- Humans, United States, Colorectal Neoplasms diagnosis, Hispanic or Latino
- Abstract
Colorectal cancer (CRC) is one of the leading cancer killers in the U.S. despite the vast array of screening and detection methods available. The U.S. Hispanic population, in particular, reflects low rates of CRC screening. In order to get a comprehensive understanding of barriers to CRC screening in this population, an integrative review was performed in PubMed and CINAHL from 2002 to 2012; eight studies on various barriers to CRC screening among the U.S. Hispanic population were examined. Our findings recognized fear, cost, and lack of awareness as common barriers to CRC screening and more specific barriers applicable to the Hispanic population being low literacy/educational levels and lack of provider recommendations. Analysis indicated culturally-specific barriers to CRC screening among Hispanics, the largest being limited English proficiency. The review demonstrated a need for additional targeted approaches, awareness education, and provider training as ways to increase CRC screening in Hispanics., (© 2013.)
- Published
- 2013
- Full Text
- View/download PDF
28. Adolescent egocentrism and cognitive functioning during late adolescence.
- Author
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Rycek RF, Stuhr SL, McDermott J, Benker J, and Swartz MD
- Subjects
- Adolescent, Adult, Female, Humans, Male, Adolescent Behavior, Cognition, Ego
- Abstract
The relationship between adolescent egocentrism and post-formal thinking was examined in 163 college undergraduates. Participants were administered the Imaginary Audience Scale and the Social Paradigm Belief Inventory. Results showed that females had higher levels of adolescent egocentrism than did males. A weak negative correlation between egocentrism and cognitive reasoning was found for females during late adolescence. The findings are consistent with Peterson and Roscoe's (1991) study of egocentrism in older adolescent females.
- Published
- 1998
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