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12 results on '"Stucki-Buchli, Brigitte"'

3. Illuminating a Phytochrome Paradigm – a Light-Activated Phosphatase in Two-Component Signaling Uncovered

Author

Multamäki, Elina, primary, Nanekar, Rahul, additional, Morozov, Dmitry, additional, Lievonen, Topias, additional, Golonka, David, additional, Yuan Wahlgren, Weixiao, additional, Stucki-Buchli, Brigitte, additional, Rossi, Jari, additional, Hytönen, Vesa P., additional, Westenhoff, Sebastian, additional, Ihalainen, Janne A., additional, Möglich, Andreas, additional, and Takala, Heikki, additional

Published
2020
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4. Effect of viscogens on the kinetic response of a photoperturbed allosteric protein.

Author

Waldauer, Steven A., Stucki-Buchli, Brigitte, Frey, Lukas, and Hamm, Peter

Subjects
*PHOTOCHEMISTRY, *ALLOSTERIC proteins, *CHEMICAL kinetics, *CONFORMATIONAL analysis, *VISCOSITY, *GLYCERIN
Abstract

By covalently binding a photoswitchable linker across the binding groove of the PDZ2 domain, a small conformational change can be photo-initiated that mimics the allosteric transition of the protein. The response of its binding groove is investigated with the help of ultrafast pump-probe IR spectroscopy from picoseconds to tens of microseconds. The temperature dependence of that response is compatible with diffusive dynamics on a rugged energy landscape without any prominent energy barrier. Furthermore, the dependence of the kinetics on the concentration of certain viscogens, sucrose, and glycerol, has been investigated. A pronounced viscosity dependence is observed that can be best fit by a power law, i.e., a fractional viscosity dependence. The change of kinetics when comparing sucrose with glycerol as viscogen, however, provides strong evidence that direct interactions of the viscogen molecule with the protein do play a role as well. This conclusion is supported by accompanying molecular dynamics simulations. [ABSTRACT FROM AUTHOR]

Published
2014
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5. 2D-IR Spectroscopy of an AHA Labeled Photoswitchable PDZ2 Domain

Author

Stucki-Buchli, Brigitte, Johnson, Philip J M, Bozovic, Olga, Zanobini, Claudio, Koziol, Klemens L, Hamm, Peter, Gulzar, Adnan, Wolf, Steffen, Buchenberg, Sebastian, Stock, Gerhard, University of Zurich, and Hamm, Peter

Subjects
10120 Department of Chemistry, 540 Chemistry, 1606 Physical and Theoretical Chemistry
Published
2017

7. 2D-IR Spectroscopy of an AHA Labeled Photoswitchable PDZ2 Domain

Author

Stucki-Buchli, Brigitte, Johnson, Philip J. M., Bozovic, Olga, Zanobini, Claudio, Koziol, Klemens L., Hamm, Peter, Gulzar, Adnan, Wolf, Steffen, Buchenberg, Sebastian, and Stock, Gerhard

Abstract

We explore the capability of the non-natural amino acid azidohomoalanine (AHA) as an IR label to sense relatively small structural changes in proteins with the help of 2D IR difference spectroscopy. To that end, we AHA-labeled an allosteric protein (the PDZ2 domain from human tyrosine-phosphatase 1E) and furthermore covalently linked it to an azobenzene-derived photoswitch as to mimic its conformational transition upon ligand binding. To determine the strengths and limitations of the AHA label, in total six mutants have been investigated with the label at sites with varying properties. Only one mutant revealed a measurable 2D IR difference signal. In contrast to the commonly observed frequency shifts that report on the degree of solvation, in this case we observe an intensitychange. To understand this spectral response, we performed classical MD simulations, evaluating local contacts of the AHA labels to water molecules and protein side chains and calculating the vibrational frequency on the basis of an electrostatic model. Although these simulations revealed in part significant and complex changes of the number of intraprotein and water contacts upon trans–cisphotoisomerization, they could not provide a clear explanation of why this one label would stick out. Subsequent quantum-chemistry calculations suggest that the response is the result of an electronic interaction involving charge transfer of the azido group with sulfonate groups from the photoswitch. To the best of our knowledge, such an effect has not been described before.

Published
2024
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8. Chromophore–Protein Interplay during the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy

Author

Ihalainen, Janne A., primary, Gustavsson, Emil, additional, Schroeder, Lea, additional, Donnini, Serena, additional, Lehtivuori, Heli, additional, Isaksson, Linnéa, additional, Thöing, Christian, additional, Modi, Vaibhav, additional, Berntsson, Oskar, additional, Stucki-Buchli, Brigitte, additional, Liukkonen, Alli, additional, Häkkänen, Heikki, additional, Kalenius, Elina, additional, Westenhoff, Sebastian, additional, and Kottke, Tilman, additional

Published
2018
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10. Coordination of the biliverdin D-ring in bacteriophytochromes

Author

Lenngren, Nils, primary, Edlund, Petra, additional, Takala, Heikki, additional, Stucki-Buchli, Brigitte, additional, Rumfeldt, Jessica, additional, Peshev, Ivan, additional, Häkkänen, Heikki, additional, Westenhoff, Sebastian, additional, and Ihalainen, Janne A., additional

Published
2018
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11. 2D-IR Spectroscopy of an AHA Labeled Photoswitchable PDZ2 Domain

Author

Stucki-Buchli, Brigitte, primary, Johnson, Philip J. M., additional, Bozovic, Olga, additional, Zanobini, Claudio, additional, Koziol, Klemens L., additional, Hamm, Peter, additional, Gulzar, Adnan, additional, Wolf, Steffen, additional, Buchenberg, Sebastian, additional, and Stock, Gerhard, additional

Published
2017
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