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The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. This article analyses the discursive configuration of a widespread assumption – that the event of climate emergency should be met with some form of concerted collective action. Beginning with references to the concept of ‘greenwashing’, it goes on to note the temporal framing of climate disaster and the imperative agency advocated in the types of address used by news organisations, climate scientists and bodies (like the United Nations) devoted to global governance. Standard media practices, which serve a vital function in circulating meaning, are also discussed, on the basis that, while they seem to advocate timely intervention, they also disassociate environmental awareness from the radical measures needed to mitigate disaster, displaying an ambiguous or (in some cases) hostile attitude to the activist forces that could drive recuperative transformations in climate policy. I argue that effective mitigation is deferred for three interrelated reasons: first, because of the recurrent emphasis placed on momentous (future) tipping points, which obscures a more insidious and rapid deterioration in the present;1 second, the preeminent tendency of mediated climate discourse to call for ‘leadership’ on issues that a deeply compromised political establishment cannot fully address; and third, state/corporate collusion,2 which makes a show of balancing competing demands (economic growth and zero emissions, for example), but is actually committed to a criminal model of industrial enterprise. Taken together, these factors – combined with the neglect of subaltern and alternative voices and the instinctive journalistic deference shown to elite social actors and their ‘authoritative’ utterances – help replicate the material/ideological structures of the social order and its hierarchical distinction between, on the one hand, leadership figures and, on the other, an infantilised public supposedly in need of rational direction. The article substantiates these observations by analysing the PR strategies of the oil and gas company, bp, while distinguishing between greenwashing critiques and the approach advocated here, which recommends the delegitimisation of corporate discourse on the basis that corporations have no right to engage in what is in effect a form of political communication. The conclusion to this piece suggests that, if the span of human life on earth has indeed been circumscribed by climate breakdown, then the emergency is political, and fighting for an immediate and ‘immoderate’ form of social justice is the only way to address a crisis that elite social actors seem determined to ignore, misrepresent or monetise.

Dust present in poultry houses can disseminate bacteria in air and deposit them on surfaces. This study evaluated bacteria in settled dust during growout of broilers from 2 flocks (Flocks A and B). Dust samples for bacteria analyses were obtained during 6 wk of growout (Flocks A and B) and 1 wk after flock termination (Flock B) by environmental swabbing and collecting dust in petri dishes from multiple locations inside the poultry house. For weekly swabbing, dust deposited during each wk of the sampling period (noncumulatively, n = 12/wk) and cumulatively (n = 12/wk) throughout the sampling period was collected. Swabbed dust samples were analyzed for counts (log10 CFU/28 cm2) of aerobic bacteria, E. coli, coliforms, and Salmonella recovery. For petri dish dust collection, dust was collected in weekly and bi-weekly time spans during the sampling period and then analyzed for Salmonella recovery. Data were analyzed by one-way ANOVA and Fisher's Exact Test and means were separated using LSD. Only aerobic plate counts changed over time in dust during growout (Flocks A and B; P < 0.0001). In noncumulatively settled dust, aerobic bacteria (Flocks A and B; P < 0.0001), E. coli (Flock A; P = 0.0432), and coliforms (Flock B; P = 0.0303) varied during growout with peak counts on wk 5 or wk 6, wk 4, and wk 4, respectively, after bird placement. Salmonella recovery did not vary in cumulatively (3/72, 10/84) and noncumulatively (0/12, 10/84) settled dust during growout in both flocks. In dust sampled by bi-weekly collection in petri dishes, Salmonella recovery was highest (5/6) between wk 2 to wk 4 for Flock B (P = 0.0118). Overall, this study displayed that settled dust bacteria levels can fluctuate during broiler growout, and dust can contain Salmonella.

Foodborne illness due to the consumption of contaminated products continues to be a serious public health issue. Bacteriophages might provide a natural and effective way to control and reduce the pathogenic bacterial population on food products. Researchers have conducted various experiments to prove their effectiveness against different pathogens and their ability to act as a natural intervention to control pathogen populations, especially in the food industry. In this study, a cocktail of bacteriophages (phages) was added to wash water in the presence of a high organic load along with commercially used sanitizers (chlorine or Sanidate 5.0) to study the efficacy of the phage–sanitizer combination in the challenge water. It was determined that in the absence of organic loads, the sanitizer by itself or the combination with phages significantly (p < 0.001) reduced the contamination by 3.00–5.00 log CFU/mL. In the presence of organic loads, the sanitizer by itself did not contribute to a significant reduction (p > 0.05) compared to the control. However, the sanitizer–phage combination led to a 3.00-log and 6.00-log reduction (p < 0.001) of the pathogen at the end of 3 and 6 h, respectively, in the presence of high organic loads. Therefore, utilizing a combination treatment (phage–sanitizer) might be one solution to reduce pathogen contamination in the food industry, especially the fresh produce industry, thus providing safe food for consumption.

The presence of Salmonella in air of poultry houses has been previously confirmed. Therefore, it is important to investigate the entry of Salmonella into broilers through air. The present study aimed to evaluate different levels of Salmonella Enteritidis aerosol inoculations in broiler chicks for colonization of ceca, trachea, and liver/spleen and persistence over time. In three independent trials, 112 1-d old birds were randomly divided into four groups (n=28/group). On d 1 of age, one group was exposed to an aerosol of sterile saline and the remaining three groups were exposed to an aerosol generated from one of three doses (103, 106, or 109 CFU/mL) of S. Enteritidis inoculum. Aerosol exposure time was 30 min/group and was performed using a nebulizer. On d 3, 7, 14, and 21 of age, ceca, trachea, and liver/spleen were aseptically removed. Ceca were cultured for Salmonella counts (log10 CFU/g) and all tissues were cultured for Salmonella prevalence. All tissues from the control group were Salmonella negative for all sampling days. On sampling d 3 and 7, ceca Salmonella counts were highest (5.14 and 5.11, respectively) when challenged with 109 Salmonella (P ≤ 0.0281). Ceca Salmonella counts increased from d 3 (2.43) to d 7 (4.43), then remained constant when challenged at 103 Salmonella, and counts decreased over time for all other groups. Tissue Salmonella prevalence increased with increasing challenge levels at all sampling timepoints (P ≤ 0.0213). Salmonella prevalence was low (0/18 to 4/18) and did not change over time following 103 Salmonella challenge (P ≥ 0.2394). Prevalence decreased over time in ceca and trachea following 106 and 109 Salmonella challenge (P ≤ 0.0483). Liver/spleen Salmonella prevalence increased from d 3 (13/18) to d 14 (18/18) and then decreased at d 21 (10/18) in birds exposed to an aerosol of 109 Salmonella but remained constant over time for rest of the Salmonella inoculated groups. Overall, this study demonstrated the Salmonella colonization and persistence in different tissues in broilers following exposure to aerosolized Salmonella.

This illustrated chapter analyses the political character and contested ‘thematic framing’ of Spain’s Huelga General Feminista, a multi-faceted protest/labor event organized since 2017 on International Women’s Day (Campillo, 2019: 253; Comisión Feminista, 2019). In the course of this discussion, I follow the Spanish practice of marking the political significance of an occasion by combining its numeric designation with the letter of the month. In this case, International Women’s Day appears as ‘8M’ (8 March), while the organized withdrawal of industrial/domestic labor associated with 8M is, for the sake of convenience, called Huelga General Feminista (HGF), though in interviews, the organizers of these events tend to refer to the ‘Feminist Strike’, while the state-owned broadcaster RTVE1 identified the day as ‘8M: Huelga General’.

Fruits and vegetables are high in nutrients that are essential for a healthy lifestyle. However, they also harbor an extensive array of microorganisms such as bacteria, which can be beneficial, neutral, or pathogenic. Foodborne pathogens can contaminate produce at any stage from the farm to the consumer’s table. Appropriate washing techniques using sanitizers can reduce the risk of pathogen contamination. Issues related to maintaining concentration, efficacy, and other problems have been a challenge for the food industry and, when left unresolved, have led to different outbreaks of foodborne illnesses. In this study, the efficacy of a lytic bacteriophage cocktail was examined for its ability to infect and reduce the contamination of Escherichia coli O157:H7 (E. coli O157:H7), in media with a high organic load, using a microplate technique. The study was conducted for 3 h to determine if the bacteriophage cocktail could reduce the pathogen in the presence of a high organic load. A significant (p &lt, 0.05) reduction in the population of E. coli O157:H7 was observed, representing a 99.99% pathogen reduction at the end of 3 h. Fresh spinach leaves were washed in sterile potable or organic water (~9000 ppm organic load) containing E. coli O157:H7 and a bacteriophage cocktail to study the effectiveness of bacteriophages against the foodborne pathogen. Results indicated that the bacteriophage significantly (p &lt, 0.05) reduced the contamination of E. coli O157:H7 in both situations. The study also demonstrated the bacteriophages’ ability to infect and reduce the pathogen in an organic-rich environment. This characteristic differs from commercially available sanitizers that have demonstrated a tendency to bind with the available organic load. Thus, these studies highlight the advantage of employing bacteriophages during produce wash to eliminate foodborne pathogen contamination on fruits and vegetables.

Chlamydia suis is an important, highly prevalent, and diverse obligate intracellular pathogen infecting pigs. In order to investigate the prevalence and diversity of C. suis in the U.S., 276 whole blood samples from feral swine were collected as well as 109 fecal swabs and 60 whole blood samples from domestic pigs. C. suis-specific peptide ELISA identified anti-C. suis antibodies in 13.0% of the blood of feral swine (26/276) and 80.0% of the domestic pigs (48/60). FRET-qPCR and DNA sequencing found C. suis DNA in 99.1% of the fecal swabs (108/109) and 21.7% of the whole blood (13/60) of the domestic pigs, but not in any of the assayed blood samples (0/267) in feral swine. Phylogenetic comparison of partial C. suis ompA gene sequences and C. suis-specific multilocus sequencing typing (MLST) revealed significant genetic diversity of the C. suis identified in this study. Highly genetically diverse C. suis strains are prevalent in domestic pigs in the USA. As crowding strongly enhances the frequency and intensity of highly prevalent Chlamydia infections in animals, less population density in feral swine than in domestic pigs may explain the significantly lower C. suis prevalence in feral swine. A future study is warranted to obtain C. suis DNA from feral swine to perform genetic diversity of C. suis between commercial and feral pigs.

Leptospirosis is a widespread zoonosis and has been recognized as a re-emerging infectious disease in humans and a variety of wild and domestic animal species. In order to understand the prevalence and diversity of Leptospira spp. in feral pig populations of Alabama, we trapped 315 feral pigs in Bullock County east-central Alabama, and collected 97 environmental samples from riparian areas in Bullock County and Macon County east-central Alabama. Two previously published PCRs followed by DNA sequencing and BLASTn were performed to identify pathogenic Leptospira species in the kidney of feral pigs (3.2%, 10/315) as well as environmental samples collected from the habitats of feral pigs (2.1%, 2/97), but not in the whole blood samples (n = 276) or spleen (n = 51). An ELISA determined that 44.2% of serum samples (122/276) were antibody-positive for Leptospira. The identification of two pathogenic Leptospira species from environmental samples and the high sero-positivity in feral pigs suggests potential pathogen shedding from feral pigs to environments, and to humans and domestic animals. In order to better understand the risk to human health associated with feral swine presence, further studies are warranted to explore the interrelationship between Leptospira spp. shedding in the urine of feral pigs and bacterial culture to explore pathogenicity. Multi-locus sequencing typing (MLST) and microscopic agglutination tests (MAT) should be performed in future studies to make a definite determination of pathogenic Leptospira in feral pigs in Alabama.

The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p &lt, 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p &lt, 0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential use of phage cocktail and sanitizer synergistically to enhance sanitation operations in the food industry.

Flies are well-known vectors of bacterial pathogens, but there are little data on their role in spreading microbial community and antimicrobial resistance. In this study, we compared the bacterial community, antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in flies with those in the feces of sympatric animals. A 16S rRNA-based microbial analysis identified 23 bacterial phyla in fecal samples and 25 phyla in flies; all the phyla identified in the fecal samples were also found in the flies. Bray–Curtis dissimilarity analysis showed that the microbiota of the flies were more similar to the microbiota of the feces of their sympatric animals than those of the feces from the three other animal species studied. The qPCR array amplified 276 ARGs/MGEs in fecal samples, and 216 ARGs/MGEs in the flies, while 198 of these genes were identified in both flies and feces. Long-term studies with larger sample numbers from more geospatially distinct populations and infection trials are indicated to further evaluate the possibility of flies as sentinels for antimicrobial resistance.

Dust present in poultry houses can contain high concentrations of microorganisms and has the potential to include pathogens from the litter. The objective of this study was to examine in vitro the potential for litter to dust transfer of aerobic bacteria, Salmonella, E. coli, and coliforms, and the role of the litter moisture on this process. Poultry litter was inoculated with 102 to 109 CFU/mL of Salmonella Typhimurium to evaluate litter to dust transfer of bacteria (Experiment 1). To evaluate the effect of litter moisture on litter to dust microbial transfer (Experiment 2), litter was inoculated with 109 S. Typhimurium with increasing amounts of sterilized water added for moisture adjustment. Dust was generated by blowing air in a direct stream onto inoculated litter while simultaneously collecting dust through impingement. Following litter and dust sample collection, microbial analyses for aerobic plate counts (APC), Salmonella, E. coli, and coliforms were conducted. Both experiments were repeated 5 times and their data analyzed by one-way ANOVA and simple logistic regression. In Experiment 1, APC of litter (log10 CFU/g) and dust samples (log10 CFU/L) were 10.55 and 4.92, respectively. Salmonella ranged from 1.70 to 6.16 log10 CFU/g in litter and only one dust sample had 1.10 log10 CFU/L of Salmonella. As Salmonella levels in litter increased, the probability of obtaining a dust Salmonella positive result also increased. In Experiment 2, attained moisture percentages were 13.0, 18.2, 23.0, 28.2, and 33.3%. Litter recovery for APC, Salmonella, E. coli, and coliforms counts did not differ (P > 0.05) with increasing moisture levels. Dust sample bacterial counts significantly decreased with increasing moisture levels (P < 0.0001). Results from this in vitro study indicate that there is potential for Salmonella to be present in generated dust and the higher levels of Salmonella in litter increase the likelihood of detecting Salmonella in dust. Additionally, with higher litter moisture percentage, prevalence of Salmonella in generated dust was decreased.

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. This article examines the ways in which executive authority and media organizations categorize the spectacle of public violence and disaster, with particular reference to an event (the Germanwings crash in 2015) where large-scale fatalities were purposely caused. On occasions when a perpetrator commits multiple killings (acting impersonally but with “malice aforethought,” and usually against civilian victims), the immediate question appears to be whether or not the incident should be classified as a terrorist attack. This is especially the case during periods when mass or individual assaults are prominent in the public domain. The article examines the problems inherent in the uses of unstable or contested linguistic definitions, which typify the family of terms that include both the act of terrorizing individuals, groups, and wider polities, and the supposedly political practice known as terrorism.

Background: Antimicrobial resistance is rising globally at an alarming rate. While multiple active surveillance programs have been established to monitor the antimicrobial resistance, studies on the environmental link to antimicrobial spread are lacking. Methods: A total of 493 flies were trapped from a dairy unit, a dog kennel, a poultry farm, a beef cattle unit, an urban trash facility and an urban downtown area to isolate Escherichia coli, Klebsiella pneumoniae and Staphylococcus spp. for antimicrobial susceptibility testing and molecular characterization. Results: E. coli, K. pneumoniae and coagulase-negative Staphylococcus were recovered from 43.9%, 15.5% and 66.2% of the houseflies, and 26.0%, 19.2%, 37.0% of the blowflies, respectively. In total, 35.3% of flies were found to harbor antimicrobial-resistant bacteria and 9.0% contained multidrug-resistant isolates. Three Staphylococcus aureus isolates were recovered from blowflies while three extended spectrum beta lactamase (ESBL)-carrying E. coli and one ESBL-carrying K. pneumoniae were isolated from houseflies. Whole genome sequencing identified the antimicrobial resistance genes blaCMY-2 and blaCTXM-1 as ESBLs. Conclusion: Taken together, our data indicate that flies can be used as indicators for environmental contamination of antimicrobial resistance. More extensive studies are warranted to explore the sentinel role of flies for antimicrobial resistance.

Background: Antimicrobial resistance is rising globally at an alarming rate. While multiple active surveillance programs have been established to monitor the antimicrobial resistance, studies on the environmental link to antimicrobial spread are lacking. Methods: A total of 493 flies were trapped from a dairy unit, a dog kennel, a poultry farm, a beef cattle unit, an urban trash facility and an urban downtown area to isolate Escherichia coli, Klebsiella pneumoniae and Staphylococcus spp. for antimicrobial susceptibility testing and molecular characterization. Results: E. coli, K. pneumoniae and coagulase-negative Staphylococcus were recovered from 43.9%, 15.5% and 66.2% of the houseflies, and 26.0%, 19.2%, 37.0% of the blowflies, respectively. In total, 35.3% of flies were found to harbor antimicrobial-resistant bacteria and 9.0% contained multidrug-resistant isolates. Three Staphylococcus aureus isolates were recovered from blowflies while three extended spectrum beta lactamase (ESBL)-carrying E. coli and one ESBL-carrying K. pneumoniae were isolated from houseflies. Whole genome sequencing identified the antimicrobial resistance genes blaCMY-2 and blaCTXM-1 as ESBLs. Conclusion: Taken together, our data indicate that flies can be used as indicators for environmental contamination of antimicrobial resistance. More extensive studies are warranted to explore the sentinel role of flies for antimicrobial resistance.

This Introduction, 14,500 words in length, is intended as a substantial intervention debates about the role of journalism in 'democratic' cultures. It was written in order to provide context for an edited book of 14 chapters, so had to be produced once all this material had been through a final edit.

According to data from the Surveillance, Epidemiology, and End Results (SEER) program in the United States, approximately 15% of men will be diagnosed with prostate cancer during their lifetimes. Over the past 15 years, the battle against prostate cancer has been joined by researchers and practitioners who have used a wide variety of operations research (OR) models and methods to investigate decisions involving screening, detection, and treatment of prostate cancer. We provide a narrative review of articles falling into the following five categories: decision analysis, machine learning, optimization, simulation, and statistics. We identified a total of 523 archival journal articles describing the use of methods in these categories in the context of prostate cancer since 2000. We categorize and annotate 49 of these articles in order to provide representative examples of the use of OR models and methods in each of these areas. We conclude with a summary of the trends in research using OR methods in the context of prostate cancer over the past 15 years, and a discussion about how these trends will influence future research.

Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in nine provinces of China, and 1,696 cloacal and 1,647 oropharyngeal samples from poultry (n = 1,836) at live-bird markets in 24 provinces. The mcr-1 prevalences in pigs (79.2%) and geese (71.7%) were significantly higher than in chickens (31.8%), ducks (34.6%) and pigeons (13.1%). The mcr-2 prevalence in pigs was 56.3%, significantly higher than in chickens (5.5%), ducks (2.3%), geese (5.5%) and pigeons (0%). The mcr-3 prevalences in pigs (18.7%), ducks (13.8%) and geese (11.9%) were significantly higher than in chickens (5.2%) and pigeons (5.1%). In total, 173 pigs and three chickens were positive for all three mcr genes. The prevalences of the mcr were significantly higher in nasal/oropharyngeal swabs than in the anal /cloacal swabs. Phylogenetic studies identified 33 new mcr-2 variants and 12 new mcr-3 variants. This study demonstrates high prevalences of mcr in pigs and poultry in China, and indicates there is need for more thorough surveillance and control programs to prevent further selection of colistin resistance.

Antimicrobial resistance to colistin has emerged worldwide threatening the efficacy of one of the last-resort antimicrobials used for the treatment of multidrug-resistant Enterobacteriaceae infection in humans. In this study, we investigated the presence of colistin resistance genes (mcr-1, mcr-2, mcr-3) in Escherichia coli strains isolated from poultry and livestock collected between 2004 and 2012 in China. Furthermore, we studied the maintenance and transfer of the mcr-1 gene in E. coli after serial passages. Overall, 2.7% (17/624) of the E. coli isolates were positive for the mcr-1 gene while none were positive for the mcr-2 and mcr-3 genes. The prevalences of mcr-1 were similar in E. coli isolates from chickens (3.2%; 13/404), pigs (0.9%; 1/113) and ducks (6.8%; 3/44) but were absent in isolates from cattle (0/63). The mcr-1 gene was maintained in the E. coli after six passages (equivalent to 60 generations). In vitro transfer of mcr-1 was evident even without colistin selection. Our data indicate the presence of mcr-1 in extraintestinal E. coli from food-producing animals in China, and suggest that high numbers of the mcr-1-positive bacteria in poultry and livestock do not appear to be readily lost after withdrawal of colistin as a food additive.

This chapter examines the representations of austerity measures and anti-austerity protests in the British national press between 2011 and 2015, in order to identify some of the underlying attitudes that produced the UK's 'Brexit'vote of 2016. Based on the content and discourse analysis of a selection of news articles covering a variety of events, particular attention is devoted to the ways in which Britishness and the strength of the British state is represented in public discourse.

This chapter examines the response of the transnational liberal elite to the rise of ‘populist’ appeals, arguing that the apparent disapproval of this development is not an indication that leading establishment figures are socially progressive, but rather that they object to a form of politics that threatens their own ability to maintain the conditions needed for capitalist accumulation. The chapter examines the use of the term community, and relates this both to the rhetoric used during the UK’s 2017 General Election, and to the form of political discourse introduced by Tony Blair. It also considers the attempt to conflate the populism of Right and Left, arguing that the elite is just as prone to the use of populist tactics as its insurgent rivals.

Poultry and livestock are the most important reservoirs for pathogenic Escherichia coli and use of antimicrobials in animal farming is considered the most important factor promoting the emergence, selection and dissemination of antimicrobial-resistant microorganisms. The aim of our study was to investigate antimicrobial resistance in E. coli isolated from food animals in Jiangsu, China. The disc diffusion method was used to determine susceptibility to 18 antimicrobial agents in 862 clinical isolates collected from chickens, ducks, pigs, and cows between 2004 and 2012. Overall, 94% of the isolates showed resistance to at least one drug with 83% being resistance to at least three different classes of antimicrobials. The isolates from the different species were most commonly resistant to tetracycline, nalidixic acid, sulfamethoxazole, trimethoprim/sulfamethoxazole and ampicillin, and showed increasing resistance to amikacin, aztreonam, ceftazidime, cefotaxime, chloramphenicol, ciprofloxacin. They were least resistant to amoxicillin/clavulanic acid (3.4%) and ertapenem (0.2%). MDR was most common in isolates from ducks (44/44, 100%), followed by chickens (568/644, 88.2%), pigs (93/113, 82.3%) and cows (13/61, 21.3%). Our finding that clinical E. coli isolates from poultry and livestock are commonly resistant to multiple antibiotics should alert public health and veterinary authorities to limit and rationalize antimicrobial use in China.

This article, which focuses on a strike and ocupation that took place in Barcelona in May 2015, is part of a larger enquiry that attempts to locate public expressions of dissent in their sociopolitical, discursive and spatiotemporal context. The methods used to conduct this study include the standard collection of observational and media data, but also encompass an attempt to investigate the control of space by public and private authorities. Discursive manifestations of dissent and the arguments posed by social actors are therefore considered in conjunction with the physical impact and material setting within which goals are pursued. The case study used in this article is the workers’ occupation, in 2015, of the Telefónica building, the famous landmark identified in George Orwell’s Homage to Catalonia as the epicentre of the internecine struggle that took place within the Republican camp in 1937.