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Objectives: To examine the within- and cross-season neuromuscular fatigue responses in English Premier League U-18 academy football players., Design: Twenty-five players from the same team completed weekly countermovement jump and isometric adductor and posterior chain strength tests for a full competitive season., Methods: Global positioning system measures of training and match total, high-metabolic load and sprint distance were recorded daily and converted into exponentially weighted moving average seven- and twenty-eight-day values., Results: TD 28 (p < .001), HML 28 (p < .001), and SD 28 (p = .002) shared negative relationships with countermovement jump height, but there was no main cross-season effect (p = .137). TD 28 (p = .016) and HML 28 (p = .012) positively related to isometric posterior chain strength but there was no effect of SD 28 (p = .653), SD 7 (p = .549), TD 7 (p = .080) or HML 7 (p = .104). Cross season, isometric posterior chain strength followed a curvilinear response whereby performance increased across the beginning of the season and decreased towards the end of the season (p = .003). There was no effect of training load or time on isometric adductor strength performance (p > .094)., Conclusions: Neuromuscular status, signalled by countermovement jump height changes fluctuates across the season in response to sustained changes in training and match load, but there is no evidence of a main cross-season linear effect. Total training and match volume exert moderating effects on the internal training load dose, and in-turn, important moderating effects on neuromuscular fatigue, particularly when composed of high volumes of high-intensity and sprint distances. The posterior chain might be particularly susceptible to fatigue towards the end of the season., Competing Interests: Declaration of interest statement All authors report that they have no conflicting interests., (Copyright © 2024. Published by Elsevier Ltd.)

We examined the sensitivity and time-course of recovery of neuromuscular and perceptual player monitoring measures to U-18 English Premier League academy football match play. Eighteen players performed maximal posterior chain, hip adductor and abductor isometric strength tests, countermovement jumps (CMJ) and provided self-report wellbeing scores around eight competitive league home games: 1 day before (MD-1), pre-match (MD-PRE), post-match (MD-POST) and two (MD+2) and three (MD+3) days post-match. A permutational multivariate analysis of variance and post hoc univariate analyses of variance were used to examine match-induced responses to monitoring variables. Between MD-1 and MD-POST, we observed small to moderate reductions to the adductor and abductor peak force and maximal impulse and IPCS peak force; small reductions to CMJ jump height (JH) (flight time), eccentric peak force and eccentric deceleration rate of force development and moderate to large reductions to perceived fatigue, muscle soreness and mood. No match-induced changes were observed for CMJ flight time: contraction time or eccentric duration. Posterior chain, abductor, CMJ and self-report measures normalised by MD+3 but adductor peak force remained compromised at MD+3 (ES = small). Posterior chain, adductor and abductor peak isometric force and maximal impulse; CMJ JH, eccentric peak force and eccentric deceleration rate of force development and perceived fatigue, muscle soreness and mood are sensitive to match-induced fatigue. Since adductor peak force remained compromised at MD+3, it is apparent that players might not achieve complete neuromuscular recovery within 3 days of match play, and that the adductor muscle group might be particularly vulnerable to match-induced fatigue., (© 2024 The Author(s). European Journal of Sport Science published by Wiley‐VCH GmbH on behalf of European College of Sport Science.)

Purpose: To examine the test-retest reliability of countermovement jump (CMJ) and isometric strength testing measures in elite-level under-18 and under-23 academy football players., Methods: A total of 36 players performed 3 maximal CMJs and isometric abductor (IABS), adductor (IADS), and posterior chain (IPCS) strength tests on 2 separate test days using dual force plates (CMJ and IPCS) and a portable strength testing device (IABS and IADS). Relative (intraclass correlation coefficient) and absolute (coefficient of variation, standard error of the measurement, and minimal detectable change [MDC%]) reliabilities for 34 CMJ, 10 IABS, 10 IADS, and 11 IPCS measures were analyzed using between-sessions best, mean, and within-session methods., Results: For all methods, relative reliability was good to excellent for all CMJ and all IADS measures and poor to good for all IABS and IPCS measures. Absolute reliability was good (ie, coefficient of variation < 10%) for 27 (best) and 28 (mean) CMJ variables and for 6 (IABS and IADS) and 2 (IPCS) isometric measures. Commonly used CMJ measures (jump height, eccentric duration, and flight-time:contraction-time ratio) had good to excellent relative reliability and an MDC% range of 14.6% to 23.7%. Likewise, commonly used isometric peak force measures for IABS, IADS, and IPCS had good to excellent relative reliability and an MDC% range of 22.2% to 26.4%., Conclusions: Commonly used CMJ and isometric strength measures had good test-retest reliability but might be limited by their MDC%. Rate-of-force-development measures (for all isometric tests) and impulse measures (IPCS) are limited by poor relative and absolute reliability and high MDC%. MDC% statistics should be considered in the context of typical responsiveness.

Biomarkers relating to player "stress balance," immunological (ie, immunoglobulin-A), and hormonal (ie, testosterone and cortisol [T:C]) status are now commonly used in football. This article is our critical review of the scientific literature relating to the response of these measures to player load and their relationships with player health. The commonly reported relationship between immunoglobulin-A and training or match load highlights its sensitivity to changes in psychophysiological stress and the increased risk of compromised mucosal immunity. This is supported by its close relationship with symptoms of upper respiratory tract infection and its association with perceived fatigue in football players. Testosterone and cortisol concentrations and the testosterone-cortisol ratio are sensitive to changes in player load, but the direction of their response is often inconsistent and is likely influenced by player training status and non-sport-related stressors. Some evidence indicates that sustained periods of high training volume can increase resting testosterone and that sustained periods of low and high training intensity can increase resting cortisol, compromising the testosterone-cortisol ratio. These findings are noteworthy, as recent findings indicate interrelationships between testosterone, cortisol, and testosterone:cortisol and perceived measures of fatigue, sleep quality, and muscle soreness in football players. Variability in individual responses suggests the need for a multivariate and individualized approach to player monitoring. Overall, we consider that there is sufficient evidence to support the use of salivary immunoglobulin-A, testosterone, cortisol, and testosterone:cortisol measures as part of a multivariate, individualized player monitoring system in professional football.

Purpose: This investigation examined the longitudinal changes and interrelationships of salivary and self-report monitoring measures across a professional football season., Methods: Measures were collected biweekly from 18 senior professional male players across a 6-wk preseason and eight 5-wk in-season mesocycles and analyzed using a linear mixed-effects model., Results: Analysis identified a small (P = 0.003) cross-season suppression of salivary immunoglobulin A, small reductions to salivary α-amylase (P = 0.047) and salivary cortisol (P = 0.007), and trivial changes to salivary testosterone (P > 0.05). The testosterone/cortisol ratio typically responded inversely to changes in player workload. Self-report measures of fatigue (P = 0.030), sleep quality (P = 0.003), and muscle soreness (P = 0.005) improved (ES = small) across the first half of the season. Fatigue and sleep measures were most consistently related to hormonal measures (R2 = 0.43-0.45). For these relationships, increases in cortisol were associated with compromised self-report responses, whereas increases in testosterone/cortisol were associated with improved responses. Nonlinear relationships were identified for fatigue with immunoglobulin A (P = 0.017; ES = trivial) and testosterone (P = 0.012; ES = trivial), for sleep quality with testosterone (P < 0.001; ES = trivial), for muscle soreness with testosterone (P = 0.012; ES = trivial), and for the self-report inventory sum with testosterone (P = 0.027; ES = trivial). For these relationships, self-report responses were optimal at mean immunoglobulin A and testosterone levels, and very low levels (-2 SD) exerted the most compromising effects., Conclusions: Players can experience a chronic cross-season suppression of mucosal immunity. Salivary immunoglobulin A, testosterone, cortisol, and testosterone/cortisol measures relate to self-report measures of fatigue, sleep quality, and muscle soreness. In-season reductions in testosterone, cortisol, and testosterone/cortisol or increases in cortisol among elite football players could be used to indicate the need for reduced workload, which might lead to improved well-being., (Copyright © 2020 by the American College of Sports Medicine.)

This investigation examined the effect of prior workload on high-intensity football match performance. Player load variables were recorded using a global positioning system and converted into composite variables: rolling season accumulated load (AL), exponentially weighted moving average acute, chronic and acute:chronic workload ratio (A:C). Match-play high-intensity performance-per-minute: accelerations (ACC), sprints, high-speed running (HSR) and high metabolic load (HMLd) distances; and situational and contextual variables were recorded for all games. Partial least squares modelling, and backward stepwise selection determined the most parsimonious model for each performance variable. Quadratic relationships of small to moderate effect sizes were identified for sprint AL and sprint performance, HSR AL and HSR performance, acute HMLd and HMLd performance, acute sprint load and ACC performance and A:C sprint load and ACC performance. Match performance was typically greatest between the mean and +1SD. High chronic HMLd, and combined acceleration and deceleration (ACC+DEC) load exerted small beneficial effects on HMLd and HSR performance, whereas high acute load exerted trivial to moderate negative effects. High sprint A:C exerted a small beneficial effect on sprint performance and playing position exerted small effects on HSR and HMLd performance. Prior workload has trivial to moderate effects on high-intensity match performance in professional players.

Graphene is a highly desirable material for a variety of applications; in the case of nanocomposites, it can be functionalized and added as a nanofiller to alter the ultimate product properties, such as tensile strength. However, often the material properties of the functionalized graphene and the location of any chemical species, attached via different functionalization processes, are not known. Thus, it is not necessarily understood why improvements in product performance are achieved, which hinders the rate of product development. Here, a commercially available powder containing few-layer graphene (FLG) flakes is characterized before and after plasma or chemical functionalization with either nitrogen or oxygen species. A range of measurement techniques, including tip-enhanced Raman spectroscopy (TERS), time-of-flight secondary ion mass spectrometry (ToF-SIMS), and NanoSIMS, were used to examine the physical and chemical changes in the FLG material at both the micro- and nanoscale. This is the first reported TERS imaging of commercially available FLG flakes of submicron lateral size, revealing the location of the defects (edge versus basal plane) and variations in the level of functionalization. Graphene-polymer composites were then produced, and the dispersion of the graphitic material in the matrix was visualized using ToF-SIMS. Finally, mechanical testing of the composites demonstrated that the final product performance could be enhanced but differed depending on the properties of the original graphitic material.

Purpose: To determine the sensitivity of a range of potential fatigue measures to daily training load accumulated over the previous 2, 3, and 4 d during a short in-season competitive period in elite senior soccer players (N = 10)., Methods: Total highspeed-running distance, perceived ratings of wellness (fatigue, muscle soreness, sleep quality), countermovement-jump height (CMJ), submaximal heart rate (HRex), postexercise heart-rate recovery (HRR), and heart-rate variability (HRV: Ln rMSSD) were analyzed during an in-season competitive period (17 d). General linear models were used to evaluate the influence of 2-, 3-, and 4-d total high-speed-running-distance accumulation on fatigue measures., Results: Fluctuations in perceived ratings of fatigue were correlated with fluctuations in total high-speed-running-distance accumulation covered on the previous 2 d (r = -.31; small), 3 d (r = -.42; moderate), and 4 d (r = -.28; small) (P < .05). Changes in HRex (r = .28; small; P = .02) were correlated with changes in 4-d total high-speed-running-distance accumulation only. Correlations between variability in muscle soreness, sleep quality, CMJ, HRR%, and HRV and total high-speed-running distance were negligible and not statistically significant for all accumulation training loads., Conclusions: Perceived ratings of fatigue and HRex were sensitive to fluctuations in acute total high-speed-running-distance accumulation, although sensitivity was not systematically influenced by the number of previous days over which the training load was accumulated. The present findings indicate that the sensitivity of morning-measured fatigue variables to changes in training load is generally not improved when compared with training loads beyond the previous day's training.

Purpose: To quantify the mean daily changes in training and match load and any parallel changes in indicators of morningmeasured fatigue across in-season training weeks in elite soccer players., Methods: After each training session and match (TL), session ratings of perceived exertion (s-RPE) were recorded to calculate overall session load (RPE-TL) in 29 English Premier League players from the same team. Morning ratings of fatigue, sleep quality, and delayed-onset muscle soreness (DOMS), as well as submaximal exercise heart rate (HRex), postexercise heart-rate recovery (HRR%), and heart-rate variability (HRV) were recorded before match day and 1, 2, and 4 d postmatch. Data were collected for a median duration of 3 wk (range 1-13) and reduced to a typical weekly cycle including no midweek match and a weekend match day. Data were analyzed using withinsubject linear mixed models., Results: RPE-TL was approximately 600 arbitrary units (AU) (95% confidence interval 546-644) higher on match day than following day (P < .001). RPE-TL progressively decreased by »60 AU per day over the 3 days before a match (P < .05). Morning-measured fatigue, sleep quality, and DOMS tracked the changes in RPE-TL, being 35-40% worse on postmatch day vs prematch day (P < .001). Perceived fatigue, sleep quality, and DOMS improved by 17-26% from postmatch day to 3 d postmatch, with further smaller (7%-14%) improvements occurring between 4 d postmatch and prematch day (P < .01). There were no substantial or statistically significant changes in HRex, HRR%, or HRV over the weekly cycle (P > .05)., Conclusions: Morning-measured ratings of fatigue, sleep quality, and DOMS are clearly more sensitive than HR-derived indices to the daily fluctuations in session load experienced by elite soccer players in a standard in-season week.

The measurement of relative physiological stress during training is important because this is the stimulus for the long-term adaptive response. Measurements of perceived exertion (RPE) have been reported to correlate with the heart rate during field-based training sessions. Nevertheless, there are few studies on how well RPE tracks with the heart rate over repeated training sessions in elite soccer players. Therefore, we aimed to quantify the within-participant correlations between variability in session-RPE (sRPE) and the heart rate in elite male soccer players, and to determine whether the playing position moderated these correlations. The field-based training of four central defenders, four wide defenders, six central midfielders, two wide midfielders and three attackers from an elite English Premier League squad were monitored over an entire in-season competitive period, giving a total of 1010 individual training sessions for study. Correlations between session-RPE and heart rates were quantified using a within-participant model. The correlation between changes in sRPE and heart rates was r = 0.75 (95% CI: 0.71-0.78). This correlation remained high across the various player positions (wide-defender, r = 0.81; central-defender, r = 0.74; wide midfielder, r = 0.70; central midfielder, r = 0.70; attacker, r = 0.84; P < 0.001). The correlation between changes in RPE and heart rates, measured during a season-long period of field-based training, is high in a sample of elite soccer players.

Purpose: To quantify the relationship between daily training load and a range of potential measures of fatigue in elite soccer players during an in-season competitive phase (17 d)., Methods: Total high-intensity-running (THIR) distance, perceived ratings of wellness (fatigue, muscle soreness, sleep quality), countermovement-jump height (CMJ), postexercise heart-rate recovery (HRR), and heart-rate variability (Ln rMSSD) were analyzed during an in-season competitive period (17 d). General linear models were used to evaluate the influence of daily fluctuation in THIR distance on potential fatigue variables., Results: Fluctuations in fatigue (r = -.51, large, P < .001), Ln rMSSD (r = -.24, small, P = .04), and CMJ (r = .23, small, P = .04) were significantly correlated with fluctuations in THIR distance. Correlations between variability in muscle soreness, sleep quality, and HRR and THIR distance were negligible and not statistically significant., Conclusions: Perceived ratings of fatigue and Ln rMSSD were sensitive to daily fluctuations in THIR distance in a sample of elite soccer players. Therefore, these particular markers show promise as simple, noninvasive assessments of fatigue status in elite soccer players during a short in-season competitive phase.

Purpose: The aim of the current study was to identify the external-training-load markers that are most influential on session rating of perceived exertion (RPE) of training load (RPE-TL) during elite soccer training., Methods: Twenty-two elite players competing in the English Premier League were monitored. Training-load data (RPE and 10-Hz GPS integrated with a 100-Hz accelerometer) were collected during 1892 individual training sessions over an entire in-season competitive period. Expert knowledge and a collinearity r < .5 were used initially to select the external training variables for the final analysis. A multivariate-adjusted within-subjects model was employed to quantify the correlations of RPE and RPE-TL (RPE × duration) with various measures of external training intensity and training load., Results: Total high-speed-running (HSR; >14.4 km/h) distance and number of impacts and accelerations >3 m/s2 remained in the final multivariate model (P < .001). The adjusted correlations with RPE were r = .14, r = .09, and r = .25 for HSR, impacts, and accelerations, respectively. For RPE-TL, the correlations were r = .11, r = .45, and r = .37, respectively., Conclusions: The external-load measures that were found to be moderately predictive of RPE-TL in soccer training were HSR distance and the number of impacts and accelerations. These findings provide new evidence to support the use of RPE-TL as a global measure of training load in elite soccer. Furthermore, understanding the influence of characteristics affecting RPE-TL may help coaches and practitioners enhance training prescription and athlete monitoring.

The realization of graphene-based, next-generation electronic applications essentially depends on a reproducible, large-scale production of graphene films via chemical vapor deposition (CVD). We demonstrate how key challenges such as uniformity and homogeneity of the copper metal substrate as well as the growth chemistry can be improved by the use of carbon dioxide and carbon dioxide enriched gas atmospheres. Our approach enables graphene film production protocols free of elemental hydrogen and provides graphene layers of superior quality compared to samples produced by conventional hydrogen/methane based CVD processes. The substrates and resulting graphene films were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and Raman microscopy, sheet resistance and transport measurements. The superior quality of the as-grown graphene films on copper is indicated by Raman maps revealing average G band widths as low as 18 ± 8 cm(-1) at 514.5 nm excitation. In addition, high charge carrier mobilities of up to 1975 cm(2)/(V s) were observed for electrons in transferred films obtained from a carbon dioxide based growth protocol. The enhanced graphene film quality can be explained by the mild oxidation properties of carbon dioxide, which at high temperatures enables an uniform conditioning of the substrates by an efficient removal of pre-existing and emerging carbon impurities and a continuous suppression and in situ etching of carbon of lesser quality being co-deposited during the CVD growth.

The aims of the present study were to: i) evaluate the agreement between estimates of high-intensity activity during soccer small-sided games (SSGs) based on running speed alone and estimated metabolic power derived from a combination of running speed and acceleration; ii) evaluate whether any bias between the 2 approaches is dependent upon playing position or drill characteristics. 3 types of SSGs (5vs5, 7vs7 and 10vs10) were completed by 26 English Premier League outfield players. A total of 420 individual drill observations were collected over the in-season period using portable global positioning system technology. High-intensity activity was estimated using the total distance covered at speeds>14.4 km · h(-1) (TS) and the equivalent metabolic power threshold of > 20 W · kg(-1) (TP). We selected 0.2 as the minimally important standardised difference between methods. High-intensity demands were systematically higher (~100%, p<0.001) when expressed as TP vs. TS irrespective of playing position and SSG. The magnitude of this difference increased as the size of SSG decreased (p<0.01) with a difference of ~200% observed in the 5vs5 SSG. A greater difference between TP and TS was also evident in central defenders compared to other positions (p<0.05) particularly during the 5vs5 SSG (~350%). We conclude that the high-intensity demands of SSGs in elite soccer players are systematically underestimated by running speed alone particularly during "small" SSGs and especially for central defenders. Estimations of metabolic power provide a more valid estimation as to the true demands of SSGs., (© Georg Thieme Verlag KG Stuttgart · New York.)

We compared measurements of high-intensity activity during field-based training sessions in elite soccer players of different playing positions. Agreement was appraised between measurements of running speed alone and predicted metabolic power derived from a combination of running speed and acceleration. Data was collected during a 10-week phase of the competitive season from 26 English Premier League outfield players using global positioning system technology. High-intensity activity was estimated using the total distance covered at speeds >14.4 km · h⁻¹ (TS) and the equivalent metabolic power threshold of >20 W · kg⁻¹ (TP), respectively. We selected 0.2 as the -minimally important standardised difference between methods. Mean training session TS was 478±300 m vs. 727±338 m for TP (p<0.001). This difference was greater for central defenders (~ 85%) vs. wide defenders and attackers (~ 60%) (p<0.05). The difference between methods also decreased as the proportion of high-intensity distance within a training session increased (R2=0.43; p<0.001). We conclude that the high-intensity demands of soccer training are underestimated by traditional measurements of running speed alone, especially in training sessions or playing positions associated with less high-intensity activity. Estimations of metabolic power better inform the coach as to the true demands of a training session., (© Georg Thieme Verlag KG Stuttgart · New York.)