1. Bacteroides thetaiotaomicron rough-type lipopolysaccharide: The chemical structure and the immunological activity
- Author
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Molly Dorothy Pither, Anna Illiano, Chiara Pagliuca, Amy Jacobson, Giuseppe Mantova, Alessia Stornaiuolo, Roberta Colicchio, Mariateresa Vitiello, Gabriella Pinto, Alba Silipo, Michael A. Fischbach, Paola Salvatore, Angela Amoresano, Antonio Molinaro, Flaviana Di Lorenzo, Pither, Molly Dorothy, Illiano, Anna, Pagliuca, Chiara, Jacobson, Amy, Mantova, Giuseppe, Stornaiuolo, Alessia, Colicchio, Roberta, Vitiello, Mariateresa, Pinto, Gabriella, Silipo, Alba, Fischbach, Michael A, Salvatore, Paola, Amoresano, Angela, Molinaro, Antonio, and Di Lorenzo, Flaviana
- Subjects
Lipopolysaccharides ,Bacteroides thetaiotaomicron ,Bacteroide ,Polymers and Plastics ,Commensal LPS ,Organic Chemistry ,Materials Chemistry ,Lipopolysaccharide (LPS) ,Structure to function ,Bacteroides ,Lipopolysaccharide ,Gut microbiota - Abstract
Bacteroides thetaiotaomicron is one of the most extensively studied symbionts of the human gut. Despite its widespread distribution among human populations, still very little is known about the role of its cell envelope in the crosstalk with the immune system. Due to the extraordinary characteristic of B. thetaiotaomicron to express multiple capsular polysaccharides on its surface, research activities focused on defining how these polymers affect immune responses. This resulted in the drawback of neglecting another immunostimulatory cell surface glycoconjugate, the lipopolysaccharide (LPS). By taking advantage of an acapsular mutant of B. thetaiotaomicron, here we describe the characterization of the structure of the rough-type LPS produced by this gut mutualist. This was made up of a mono-phosphorylated and hypoacylated lipid A and of a highly charged core oligosaccharide. In vitro studies revealed a weak ability to engage the MD-2/TLR4 pathway, while it was able to promote TLR2-mediated response.
- Published
- 2022