1. Prostaglandin D2 synthase and its post-translational modifications in neurological disorders
- Author
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Denis F. Hochstrasser, Pierre Burkhard, Pierre Lescuyer, Jean-Charles Sanchez, and Alberto Gandini
- Subjects
Gene isoform ,Male ,medicine.medical_specialty ,Clinical Biochemistry ,Stroke/cerebrospinal fluid/diagnosis ,cerebrospinal fluid, dementia, prostaglandin D2 synthase, post-translational modifications ,Disease ,Lipocalin ,Intramolecular Oxidoreductases/cerebrospinal fluid/metabolism ,Biochemistry ,cerebrospinal fluid ,Analytical Chemistry ,Cerebrospinal fluid ,Internal medicine ,post-translational modifications ,Medicine ,Dementia ,Humans ,Protein Isoforms ,Electrophoresis, Gel, Two-Dimensional ,ddc:576 ,Stroke ,Aged ,chemistry.chemical_classification ,prostaglandin D2 synthase ,Aged, 80 and over ,ddc:615 ,Protein Isoforms/cerebrospinal fluid ,biology ,business.industry ,Prostaglandin D2 synthase ,Neurodegenerative Diseases ,Neurodegenerative Diseases/cerebrospinal fluid/diagnosis ,Biological Markers/cerebrospinal fluid ,Middle Aged ,medicine.disease ,Lipocalins ,Intramolecular Oxidoreductases ,Endocrinology ,chemistry ,Immunology ,biology.protein ,Female ,business ,Glycoprotein ,Protein Processing, Post-Translational ,Biomarkers ,dementia - Abstract
Prostaglandin D2 synthase (PGDS) (beta-trace protein) is a highly abundant cerebrospinal fluid (CSF) glycoprotein. A number of studies have been performed to determine the potential value of this protein for the diagnosis of various neurological disorders. The measurement of total PGDS levels in CSF has proved marginally useful for this purpose, but promising results were obtained while investigating changes in the posttranslational modifications (PTM) pattern. Using 2-DE analysis, we previously showed that PGDS is differentially expressed in ante- and post mortem CSF samples. In the present study, we examined whether the PGDS isoforms may help to distinguish stroke and neurodegenerative disease patients from healthy subjects. The pattern of PGDS PTM was analyzed in CSF from patients with various neurological disorders (n = 44) using IEF/immunoblotting techniques. Strong alterations of this pattern were detected in patients with different forms of degenerative dementia. These findings are consistent with PGDS being altered in some neurological diseases and provide new opportunities for clinical applications.
- Published
- 2005