123 results on '"Striker LJ"'
Search Results
2. Glomerulosclerosis in mice transgenic for native or mutated growth hormone
- Author
-
Yang, C, Striker, Lj, Kopchick, Jj, Chen, Wc, Pesce, Carlo, Peten, Ep, and Striker, Ge
- Published
- 1993
3. Glomerulosclerosis and body growth are mediated by different portions of bovine growth hormone: Studies in transgenic mice
- Author
-
Yang, Cw, Striker, Lj, Pesce, Carlo, Chen, Wy, Peten, Ep, Elliot, S, Doi, T, Kopchick, Jj, and Striker, Ge
- Published
- 1993
4. The inheritance of glomerulosclerosis in mice is controlled by multiple quantitative trait loci.
- Author
-
Lenz, O, Zheng, F, Vilar, J, Doublier, S, Lupia, E, Schwedler, S, Striker, LJ, and Striker, GE
- Abstract
Background.Glomerulosclerosis, the common terminal event in chronic glomerular diseases such as diabetic nephropathy or IgA nephropathy, leads to end-stage renal disease. The considerable variation in both the risk of developing glomerulosclerosis and the rate of progression in individual patients suggest a role for genetic factors which have not been identified so far. In this study we sought to examine the mode of inheritance of glomerulosclerosis in mice. [ABSTRACT FROM PUBLISHER]
- Published
- 1998
- Full Text
- View/download PDF
5. Editorial comment. Nephron reduction in man - lessons from the Os mouse.
- Author
-
Striker, LJ
- Published
- 1998
- Full Text
- View/download PDF
6. Combined AGE inhibition and ACEi decreases the progression of established diabetic nephropathy in B6 db/db mice.
- Author
-
Zheng F, Zeng YJ, Plati AR, Elliot SJ, Berho M, Potier M, Striker LJ, and Striker GE
- Subjects
- Albuminuria drug therapy, Albuminuria mortality, Albuminuria pathology, Animals, Antihypertensive Agents pharmacology, Benzazepines pharmacology, Collagen Type IV metabolism, Diabetes Mellitus, Type 2 complications, Diabetic Nephropathies mortality, Diabetic Nephropathies pathology, Disease Progression, Drug Therapy, Combination, Female, Glycation End Products, Advanced blood, Mice, Mice, Inbred C57BL, Mice, Obese, Tetrazoles pharmacology, Valine analogs & derivatives, Valine pharmacology, Valsartan, Angiotensin-Converting Enzyme Inhibitors pharmacology, Diabetic Nephropathies drug therapy, Enalapril pharmacology, Glycation End Products, Advanced antagonists & inhibitors, Pyridoxamine pharmacology, Vitamin B Complex pharmacology
- Abstract
The accumulation of advanced glycation end products (AGE) is a key factor in diabetic nephropathy (DN). Pyridoxamine inhibits AGE formation and protects against type I DN. Herein we tested: (1) whether C57BL6 db/db mice as a model of established type II DN resembled patients treated with drugs which inhibit angiotensin II action; (2) whether pyridoxamine was effective as a single therapy; and (3) whether pyridoxamine would add to the benefit of angiotensin-converting enzyme inhibition (ACEi) by enalapril. In first set of experiments mice were treated with ACEi (benazepril) and an angiotensin II receptor blocker (valsartan) combination for 16 weeks after the onset of diabetes. In second group, mice with established DN were treated with pyridoxamine for 8 weeks. In a third set, mice with established DN were treated with pyridoxamine and enalapril combination for 16 weeks. Benazepril and valsartan combination partially prevented the development and progression of DN. Pyridoxamine treatment, as single therapy, decreased the progression of albuminuria and glomerular lesions. The combination of pyridoxamine with enalapril reduced both mortality and the progression of DN. In conclusion, (1) C57 BL6 db/db mice are a model of progressive type II DN; (2) The combination of pyridoxamine with enalapril decreased progression of type 2 DN and overall mortality. Thus, pyridoxamine could be a valuable adjunct to the current treatment of established type II DN.
- Published
- 2006
- Full Text
- View/download PDF
7. Glomerular aging in females is a multi-stage reversible process mediated by phenotypic changes in progenitors.
- Author
-
Feng Z, Plati AR, Cheng QL, Berho M, Banerjee A, Potier M, Jy WC, Koff A, Striker LJ, and Striker GE
- Subjects
- Animals, Blotting, Western, Bone Marrow Transplantation, Cell Cycle Proteins metabolism, Cell Proliferation, Cyclin-Dependent Kinase Inhibitor p21, Cyclin-Dependent Kinase Inhibitor p27, Female, Flow Cytometry, Glomerular Mesangium cytology, Hypertrophy, Immunoenzyme Techniques, Male, Matrix Metalloproteinase 2 metabolism, Mice, Mice, Inbred C57BL, Phenotype, Sclerosis, Tumor Suppressor Proteins metabolism, Aging, Glomerular Mesangium physiology, Kidney Glomerulus physiology, Stem Cells physiology
- Abstract
The glomeruli of postmenopausal C57BL6 mice, and age-matched males, show progressive hypertrophy and glomerulosclerosis. We asked whether this was a multistage process, was due to alterations in glomerular progenitors, and was reversible in female mice. Using cross bone marrow transplants (BMT) between young and old females, we found that BMT delivered a phenotype that was donor age-specific. The fact that lesions in young recipients were more severe if the donors were in late rather than early menopause suggested that new progenitor phenotypes had appeared. Postmenopausal recipients of BMT from young donors had reduced glomerular hypertrophy and sclerosis, implying that the aging lesions in females were reversible and that progenitors, rather than the local environment, determined the glomerular profile. The altered phenotype included increased extracellular matrix synthesis and decreased matrix metalloproteinase-2 levels as well as cell hypertrophy. The mechanism of the cellular hypertrophy was due to uncoupling of hypertrophy from proliferation, resulting from elevated p27 levels. Thus, glomerular hypertrophy and sclerosis in aging females is a multistage process, is reversible, and may be determined by the phenotype of bone marrow-derived progenitor cells.
- Published
- 2005
- Full Text
- View/download PDF
8. The glomerulosclerosis of aging in females: contribution of the proinflammatory mesangial cell phenotype to macrophage infiltration.
- Author
-
Zheng F, Cheng QL, Plati AR, Ye SQ, Berho M, Banerjee A, Potier M, Jaimes EA, Yu H, Guan YF, Hao CM, Striker LJ, and Striker GE
- Subjects
- Animals, Chemokine CCL5 biosynthesis, Chemokine CCL5 metabolism, Dose-Response Relationship, Drug, Female, Gene Expression Regulation, Genes, Reporter, Humans, Inflammation, Mice, Mice, Inbred C57BL, NF-kappa B metabolism, Oligonucleotide Array Sequence Analysis, Phenotype, RNA metabolism, RNA, Messenger metabolism, Receptors, Tumor Necrosis Factor, Type I biosynthesis, Receptors, Tumor Necrosis Factor, Type II biosynthesis, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Transfection, Tumor Necrosis Factor-alpha biosynthesis, Vascular Cell Adhesion Molecule-1 biosynthesis, Aging, Glomerular Mesangium pathology, Glomerulosclerosis, Focal Segmental pathology, Macrophages pathology
- Abstract
Age-associated renal changes may be an important cause of renal failure. We recently found that aged female B6 mice developed progressive glomerular lesions. This was associated with macrophage infiltration, a frequent finding in glomerulosclerosis. We used these mice as a model for studying the mechanisms of glomerular aging. We compared the gene expression profile of intact glomeruli from late postmenopausal (28-month-old) mice to that of intact glomeruli from premenopausal (5-month-old) mice. We found that inflammation-related genes, especially those expressed by activated macrophages, were up-regulated in the glomeruli of 28-month-old mice, a result correlating with the histological observation of glomerular macrophage infiltration. The mechanism for macrophage recruitment could have been stable phenotypic changes in mesangial cells because we found that mesangial cells isolated from 28-month-old mice expressed higher levels of RANTES and VCAM-1 than cells from 5-month-old mice. The elevated serum tumor necrosis factor (TNF)-alpha levels present in aged mice may contribute to increased RANTES and VCAM-1 expression in mesangial cells. Furthermore, cells from 28-month-old mice were more sensitive to TNF-alpha-induced RANTES and VCAM-1 up-regulation. The effect of TNF-alpha on RANTES expression was mediated by TNF receptor 1. Interestingly, mesangial cells isolated from 28-month-old mice had increased nuclear factor-kappaB transcriptional activity. Inhibition of nuclear factor-kappaB activity decreased baseline as well as TNF-alpha-induced RANTES and VCAM-1 expression in mesangial cells isolated from 28-month-old mice. Thus, phenotypic changes in mesangial cells may predispose them to inflammatory stimuli, such as TNF-alpha, which would contribute to glomerular macrophage infiltration and inflammatory lesions in aging.
- Published
- 2004
- Full Text
- View/download PDF
9. Development of albuminuria and glomerular lesions in normoglycemic B6 recipients of db/db mice bone marrow: the role of mesangial cell progenitors.
- Author
-
Zheng F, Cornacchia F, Schulman I, Banerjee A, Cheng QL, Potier M, Plati AR, Berho M, Elliot SJ, Li J, Fornoni A, Zang YJ, Zisman A, Striker LJ, and Striker GE
- Subjects
- Albuminuria metabolism, Animals, Blood Glucose, Cell Division, Diabetes Mellitus, Type 2 metabolism, Diabetic Nephropathies metabolism, Female, Glomerular Mesangium metabolism, Insulin metabolism, Matrix Metalloproteinase 2 metabolism, Mice, Mice, Mutant Strains, Stem Cells cytology, Albuminuria pathology, Bone Marrow Transplantation, Diabetes Mellitus, Type 2 pathology, Diabetic Nephropathies pathology, Glomerular Mesangium pathology
- Abstract
The pathologic hallmarks of diabetic nephropathy are excess mesangial extracellular matrix (ECM) and mesangial cell proliferation. We previously showed that mesangial cell phenotypic changes play an important role in the pathogenesis of diabetic nephropathy. We concluded that phenotypic changes were present in bone marrow (BM)-derived mesangial cell progenitors, as transplantation of BM from db/db mice, a model of type 2 diabetic nephropathy, transferred the db genotype and a nephropathy phenotype to naive B6 mice recipients. The recipients did not develop diabetes; however, they did develop albuminuria and glomerular lesions mirroring those in the donors (i.e., glomerular hypertrophy, increased ECM, and increased cell number with cell proliferation). We found that matrix metalloproteinase 2 (MMP-2) facilitated invasion of the mesangial cells into ECM and proliferation in vitro. Thus, increased MMP-2 activity in db/db mesangial cell progenitors may partially explain increased mesangial cell repopulation and proliferation in B6 recipients of db/db BM. In summary, BM-derived mesangial cell progenitors may play a crucial role in the development and progression of ECM accumulation and mesangial cell proliferation in this model of diabetic nephropathy in type 2 diabetes.
- Published
- 2004
- Full Text
- View/download PDF
10. Effects of ventricular unloading on apoptosis and atrophy of cardiac myocytes.
- Author
-
Schena S, Kurimoto Y, Fukada J, Tack I, Ruiz P, Pang M, Striker LJ, Aitouche A, and Pham SM
- Subjects
- Animals, Atrophy, Male, Models, Animal, Organ Size physiology, Rats, Rats, Inbred ACI, Ventricular Function physiology, Apoptosis physiology, Heart Transplantation, Heart-Assist Devices, Myocytes, Cardiac pathology, Myocytes, Cardiac physiology
- Abstract
Background: Ventricular unloading decreases cardiac ventricular mass. This loss of ventricular mass can be due to either atrophy (a reversible process) or apoptosis (an irreversible process) of the cardiac myocytes. We investigated the effect of ventricular unloading on atrophy and apoptosis of cardiac myocytes, using working and nonworking transplant heart models in rats., Materials and Methods: ACI rats underwent heterotopic heart transplantation with two different techniques to create working and nonworking cardiac grafts. Cardiac grafts were harvested at different time points after transplantation. TUNEL, caspase-3 assay, and electron microscopy were used to assess the degree of apoptosis while cellular atrophy was estimated by calculation of the cytoplasmic index (CI = mean sectional cytoplasmic area/nucleus)., Results: Ventricular mass reduction was more pronounced in nonworking than in working hearts (P < 0.05). Apoptotic index and caspase-3 activities increased in both groups, peaking at 3 days after transplantation, but were not significantly different between the two models. The cytoplasmic index was significantly lower in nonworking than in working grafts (P < 0.05)., Conclusions: These data suggest that cellular atrophy is the primary mechanism that accounts for myocardial weight reduction following ventricular unloading. The inference is that ventricular unloading by ventricular assist devices may not cause permanent loss of cardiac myocytes, thus allowing for functional recovery.
- Published
- 2004
- Full Text
- View/download PDF
11. Response to sex hormones differs in atherosclerosis-susceptible and -resistant mice.
- Author
-
Potier M, Karl M, Elliot SJ, Striker GE, and Striker LJ
- Subjects
- Animals, Aorta immunology, Collagen Type I metabolism, Collagen Type IV metabolism, Coronary Artery Disease genetics, Coronary Artery Disease immunology, Culture Techniques, Disease Susceptibility, Dose-Response Relationship, Drug, Estrogen Receptor alpha, Estrogen Receptor beta, Extracellular Matrix metabolism, Female, Gene Expression Regulation immunology, Gonadal Steroid Hormones pharmacology, Immunity, Innate, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred C3H, Aorta drug effects, Aorta metabolism, Coronary Artery Disease metabolism, Estradiol pharmacology, Progesterone pharmacology, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism
- Abstract
Genetic factors that determine the degree of susceptibility to atherosclerosis may also influence the effects of estrogens and progestins in arterial vessel disease. We examined and compared estrogen receptor (ER) and progesterone receptor (PR) expression and the effects of 17beta-estradiol (E2) and progesterone (P) on collagen synthesis and matrix metalloproteinase (MMP) activities in the aortic arch and in cultured aortic smooth muscle cells (ASMC) of atherosclerosis-susceptible (C57Bl6/J, B6) or -resistant (C3H/HeJ, C3H) mice. ERalpha, ERbeta, and PR levels were higher in the aorta and ASMC of atherosclerosis-susceptible B6 mice. In transfection studies using an estrogen response element-driven reporter plasmid, E2 elicited a >2-fold increase in luciferase activity in ASMC of B6 (B6-ASMC), which demonstrated the transcriptional activity of ER in atherosclerosis-susceptible cells. Importantly, the response of endogenous target genes to E2 and P was different in B6-ASMC and C3H-ASMC. E2 decreased collagen synthesis but had no effect on MMP activities in B6-ASMC. P decreased MMP-2 and MMP-9 activity in B6-ASMC. In contrast, E2 increased MMP-2 and decreased MMP-9 activity but had no effect on collagen synthesis in C3H-ASMC. P had no effect on collagen synthesis and MMP activity in C3H-ASMC. These differences in response to sex hormones may have important implications for women who receive hormone replacement therapy.
- Published
- 2003
- Full Text
- View/download PDF
12. Glucose induces clonal selection and reversible dinucleotide repeat expansion in mesangial cells isolated from glomerulosclerosis-prone mice.
- Author
-
Fornoni A, Lenz O, Striker LJ, and Striker GE
- Subjects
- Alleles, Animals, Apoptosis drug effects, Cell Division drug effects, Cells, Cultured, Gene Deletion, Gene Expression, Gene Expression Profiling, Gene Frequency, Glomerular Mesangium enzymology, Glomerular Mesangium pathology, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred C57BL, Mice, Inbred Strains genetics, Polymorphism, Genetic, Promoter Regions, Genetic genetics, Clone Cells, Dinucleotide Repeats, Genetic Predisposition to Disease, Glomerular Mesangium physiopathology, Glomerulosclerosis, Focal Segmental genetics, Glucose pharmacology
- Abstract
Clonal selection has been proposed as a pathogenetic mechanism in various chronic diseases, such as scleroderma, hypertension, pulmonary fibrosis, interstitial fibrosis of the kidney, atherosclerosis, and uterine leiomyomatosis. We previously found that mesangial cells from ROP mice prone to develop glomerulosclerosis changed their phenotype in response to high glucose concentrations. Here, we investigate whether clonal selection might contribute to this phenotype change. We found that in ROP mice at least two distinct mesangial cell clones exist. They are characterized by a different length of the d(CA) repeat in the MMP-9 promoter and exhibit a significantly different gene expression profile. Exposure of ROP mesangial cells to 25 mmol/l glucose for 35 days induces both clonal selection and reversible dinucleotide repeat expansion. None of these findings were present in mesangial cells isolated from C57BL/6 mice, which are not sclerosis-prone. We conclude that mesangial cell michrochimerism may be a marker for the susceptibility to glomerulosclerosis, that dinucleotide repeat expansion may be a novel mechanism for glucose-induced changes in gene expression, and that clonal selection may partially explain the change in mesangial cell phenotype in diabetes.
- Published
- 2003
- Full Text
- View/download PDF
13. The molecular basis of age-related kidney disease.
- Author
-
Zheng F, Plati AR, Banerjee A, Elliot S, Striker LJ, and Striker GE
- Subjects
- Aged, Animals, Female, Humans, Incidence, Kidney Glomerulus pathology, Postmenopause, United States epidemiology, Aging genetics, Aging pathology, Kidney Diseases epidemiology, Kidney Diseases genetics, Kidney Diseases pathology
- Abstract
Renal disease affects 11% of people in the United States over the age of 65, not including those with diabetes or hypertension. Although glomerular disease is the most common underlying etiology of age-related renal dysfunction, the cause of glomerular disease and whether it is the only contributor to renal failure are not known. Our studies in female mice show that renal disease in the postmenopausal period is associated with progressive glomerular enlargement and scarring, as well as abnormal renal function. To study the underlying causes of aging-related glomerular disease, we isolated and characterized glomerular smooth muscle (mesangial) cells from female mice of various ages. We found that the cells from older mice exhibit a variety of phenotypic changes, including increased concentrations of p27, a protein that serves to inhibit progression from the G1 to the S phase of the cell cycle. Because the bone marrow (BM) contains mesangial cell progenitors that can transfer the donor glomerular phenotype (normal or diseased) to recipients, we exchanged BM between postmenopausal and premenopausal mice and found that aging-related glomerular enlargement and scarring are transferred to young recipient glomeruli. In addition, BM from normal, young donors led to the regression of aging-related glomerular disease in postmenopausal recipients; namely, both glomerular enlargement and scarring were reduced. Thus, aging-related glomerular disease is an entity distinct from all other causes of renal disease, is characterized by phenotypic changes in mesangial cell progenitors, and is reversible when the phenotype of the progenitors is returned to normal.
- Published
- 2003
- Full Text
- View/download PDF
14. Estrogen deficiency accelerates progression of glomerulosclerosis in susceptible mice.
- Author
-
Elliot SJ, Karl M, Berho M, Potier M, Zheng F, Leclercq B, Striker GE, and Striker LJ
- Subjects
- Albuminuria, Animals, Blood Urea Nitrogen, Creatinine urine, Disease Progression, Disease Susceptibility, Female, Glomerulosclerosis, Focal Segmental urine, Kidney Glomerulus ultrastructure, Mice, Mice, Inbred Strains, Ovariectomy, Estrogens deficiency, Glomerulosclerosis, Focal Segmental pathology, Glomerulosclerosis, Focal Segmental prevention & control, Kidney Glomerulus pathology
- Abstract
Estrogen deficiency may contribute to the development and progression of glomerulosclerosis in postmenopausal women. The responsiveness to estrogens could be controlled by genetic traits related to those that determine the susceptibility to glomerular scarring. This study was undertaken to determine whether the intensity of the sclerotic response was modified by the estrogen status in sclerosis-prone ROP Os/+ mice. Ovariectomized ROP Os/+ mice developed more severe renal dysfunction and glomerulosclerosis than intact, ie, estrogen sufficient age-matched female mice. Ovariectomized ROP Os/+ exhibited increased accumulation of extracellular matrix, predominantly of laminin, and a marked distortion of the glomerular architecture. We found an increase in macrophage infiltration in the mesangium of ovariectomized ROP Os/+. Estrogen deficiency decreased glomerular estrogen receptor expression in ROP Os/+ mice, which we had previously found to be low in the parental ROP strain. Thus, although physiological estrogen levels in young ROP Os/+ mice could not prevent the development of glomerulosclerosis, estrogen deficiency accelerated the progression of glomerular scarring in this mouse strain. This suggests that estrogen replacement will slow but not prevent the progression of glomerulosclerosis. It underscores the importance of the genetic composition of individuals that determines the susceptibility to diseases as well as the response to treatment.
- Published
- 2003
- Full Text
- View/download PDF
15. Resistance to glomerulosclerosis in B6 mice disappears after menopause.
- Author
-
Zheng F, Plati AR, Potier M, Schulman Y, Berho M, Banerjee A, Leclercq B, Zisman A, Striker LJ, and Striker GE
- Subjects
- Albuminuria pathology, Animals, Blood Glucose metabolism, Blood Urea Nitrogen, Collagen Type I genetics, Collagen Type IV genetics, Creatinine blood, Female, Glomerulosclerosis, Focal Segmental genetics, Glomerulosclerosis, Focal Segmental pathology, Humans, Immunity, Innate, Insulin administration & dosage, Insulin pharmacology, Kidney growth & development, Kidney Glomerulus growth & development, Kidney Glomerulus pathology, Menopause, Mice, Mice, Inbred Strains, Transcription, Genetic, Aging physiology, Estrus immunology, Glomerulosclerosis, Focal Segmental immunology, Kidney pathology
- Abstract
The frequency of chronic renal failure increases with age, especially in women after menopause. Glomerulosclerosis is a common cause of chronic renal failure in aging. We reported that pre-menopausal female C57BL6 (B6) mice are resistant to glomerulosclerosis, irrespective of the type of injury. However, we now show that B6 mice develop progressive glomerulosclerosis after menopause. Glomerular lesions, first recognized in 18-month-old mice, consisted of hypertrophy, vascular pole sclerosis, and mesangial cell proliferation. Diffuse but moderate mesangial sclerosis and more marked hypertrophy were present at 22 months. At 28 to 30 months the glomerulosclerosis was diffuse and increased levels of type I and type IV collagen and transforming growth factor-beta 1 mRNA were present. Urine albumin excretion was significantly increased in 30-month-old mice. Mesangial cells isolated from 28-month-old mice retained their sclerotic phenotype in vitro. Comparison of the effects of uninephrectomy (Nx) in 20-month-old and 2.5-month-old mice revealed a 1.7-fold increase in urine albumin excretion, accelerated glomerulosclerosis, and renal function insufficiency in 20-month-old Nx mice, but not in 2.5-month-old Nx mice. Glycemic levels, glucose, insulin tolerance, and blood pressure were normal at all ages. Thus, B6 mice model the increased frequency of chronic renal failure in postmenopausal women and provide a model for studying the mechanism(s) of glomerulosclerosis in aging women.
- Published
- 2003
- Full Text
- View/download PDF
16. Windows on renal biopsy interpretation: does mRNA analysis represent a new gold standard?
- Author
-
Striker LJ and Striker GE
- Subjects
- Biopsy standards, Humans, Kidney pathology, Kidney Diseases genetics, Kidney Diseases pathology, RNA, Messenger genetics
- Published
- 2003
- Full Text
- View/download PDF
17. Pentosan polysulfate decreases prostate smooth muscle proliferation and extracellular matrix turnover.
- Author
-
Elliot SJ, Zorn BH, McLeod DG, Moul JW, Nyberg L, Striker LJ, and Striker GE
- Subjects
- Cell Culture Techniques, Enzyme-Linked Immunosorbent Assay, Humans, Immunohistochemistry, Male, Prostatic Hyperplasia physiopathology, Prostatic Neoplasms physiopathology, Cell Division drug effects, Enzyme Inhibitors pharmacology, Extracellular Matrix metabolism, Muscle, Smooth drug effects, Muscle, Smooth growth & development, Pentosan Sulfuric Polyester pharmacology, Prostate physiology, Prostatic Hyperplasia drug therapy
- Abstract
Benign prostatic hyperplasia (BPH) involves proliferation of smooth muscle cells and increased deposition of extracellular matrix (ECM). We recently found that pentosan polysulfate (PPS) has marked effects on growth and ECM of smooth muscle cells derived from vascular tissues. We examined smooth muscle cells cultured from human prostates and the effects of PPS on their growth and ECM production. Fragments of surgical prostatectomy specimens were diced, digested with collagenase (0.01%), and placed in culture medium supplemented with 20% fetal bovine serum. Outgrowths of elongated cells were characterized by light microscopic examination and immunohistochemical techniques by the presence of F-actin, alpha-smooth muscle actin, and myosin, which is a characteristic of smooth muscle cells. Two independent isolates were propagated, and growth curves and ECM production were assessed in the presence and absence of PPS (10 or 100 microg/ml). PPS decreased cell number beginning at day 1 and throughout the incubation period, up to 4 days. The amount of the ECM degradative enzymes, metallo-proteinases MMP-9 and MMP-2, was examined by zymography. PPS did not alter the amount of MMP-2 in the supernatants but MMP-9 was increased 234.4 +/- 17.23-fold over control cells. Tissue inhibitor of MMP (TIMPS), examined by reverse zymography, increased 200% over control. The amount of alpha I type (IV) and alpha I type (I) collagen released in the supernatant, measured by ELISA, significantly decreased in PPS-treated cultures. In conclusion, we found that the administration of PPS decreased proliferation as well as ECM production in prostate smooth muscle. Since smooth muscle proliferation and ECM are involved in the pathophysiology of BPH, PPS may have therapeutic potential.
- Published
- 2003
- Full Text
- View/download PDF
18. Regulation of estrogen receptors and MMP-2 expression by estrogens in human retinal pigment epithelium.
- Author
-
Marin-Castaño ME, Elliot SJ, Potier M, Karl M, Striker LJ, Striker GE, Csaky KG, and Cousins SW
- Subjects
- Aged, Aged, 80 and over, Blotting, Western, Cell Culture Techniques, Estrogen Antagonists pharmacology, Estrogen Receptor alpha, Estrogen Receptor beta, Female, Fulvestrant, Gene Expression Regulation drug effects, Humans, Male, Matrix Metalloproteinase 2 metabolism, Middle Aged, NF-kappa B antagonists & inhibitors, Pigment Epithelium of Eye metabolism, Proline pharmacology, RNA, Messenger metabolism, Receptors, Estrogen metabolism, Reverse Transcriptase Polymerase Chain Reaction, Thiocarbamates pharmacology, Transfection, Estradiol analogs & derivatives, Estradiol pharmacology, Matrix Metalloproteinase 2 genetics, Pigment Epithelium of Eye drug effects, Proline analogs & derivatives, Receptors, Estrogen genetics
- Abstract
Purpose: Age-related macular degeneration (ARMD) is characterized by progressive thickening and accumulation of various lipid-rich extracellular matrix (ECM) deposits under the retinal pigment epithelium (RPE). ECM dysregulation probably contributes to the pathologic course of ARMD. By activating estrogen receptors (ERs), estrogens regulate the expression of genes relevant in the turnover of ECM, among them matrix metalloproteinase (MMP)-2. Estrogen deficiency may predispose to dysregulated synthesis and degradation of ECM, leading to accumulation of collagens and other proteins between the RPE and its basement membrane. The purposes in the current study were to confirm the expression of ERs in human RPE, to elucidate whether these ERs are functional, and to test whether 17beta-estradiol (E(2)) regulates expression of ERs and MMP-2., Methods: Expression of ERs was examined in freshly isolated human RPE monolayer and in cultured human RPE cells, by using total RNA for RT-PCR and protein extracts for Western blot analysis. Supernatants were collected from freshly isolated human RPE and from cultured human RPE to assess MMP-2 activity by zymography and protein expression by Western blot. The transcriptional activity of ERs was studied in transfection experiments with an estrogen-responsive reporter construct. All these studies were preformed in the presence or absence of E(2) (10(-11) and 10(-7) M)., Results: Human RPE isolated from female and male individuals expressed both ER subtypes alpha and beta at the mRNA and protein levels. Treatment of cultured RPE cells with 10(-10) M E(2) increased expression of mRNA and protein of both receptor subtypes. E(2) (10(-10) M) also increased MMP-2 activity (approximately 2.2-fold) and protein expression (approximately 2.5-fold). In contrast, there was no change in ER levels and MMP-2 activity at higher E(2) concentrations (10(-8) M), compared with baseline. Preincubation of cells with 10(-7) M pyrrolidinedithiocarbamate (PDTC), an inhibitor of nuclear factor (NF)-kappaB, abolished the increase in MMP-2 activity and protein expression induced by E(2) at 10(-10) M., Conclusions: Both ER subtypes are expressed in RPE and regulated in a dose-dependent fashion by E(2). Estrogens similarly regulate MMP-2. This estrogen-induced effect is, at least in part, mediated through NF-kappaB. These data support the hypothesis that estrogens may exert biological function in RPE through ERs and that estrogen deficiency or excess may cause dysregulation of molecules that influence the turnover of ECM in Bruch's membrane associated with ARMD.
- Published
- 2003
- Full Text
- View/download PDF
19. Association of a decreased number of d(CA) repeats in the matrix metalloproteinase-9 promoter with glomerulosclerosis susceptibility in mice.
- Author
-
Fornoni A, Wang Y, Lenz O, Striker LJ, and Striker GE
- Subjects
- Animals, Base Sequence genetics, Cells, Cultured, Dinucleotide Repeats, Mice, Molecular Sequence Data, Promoter Regions, Genetic drug effects, RNA, Messenger metabolism, Tetradecanoylphorbol Acetate pharmacology, Transcription, Genetic genetics, Genetic Predisposition to Disease genetics, Glomerulosclerosis, Focal Segmental genetics, Matrix Metalloproteinase 9 genetics, Polymorphism, Genetic genetics, Promoter Regions, Genetic genetics
- Abstract
The genetic background plays an important role in the development of progressive glomerulosclerosis. However, no marker is available for the reliable prediction of genetic susceptibility to glomerulosclerosis. Because matrix metalloproteinase-9 (MMP-9) levels are decreased in models of glomerulosclerosis and MMP-9 promoter polymorphism has been observed among patients with diabetic nephropathy, MMP-9 could be one such marker. The object of this study was to determine whether MMP-9 promoter polymorphism was associated with altered MMP-9 expression in mesangial cells (MC) from two mouse strains, i.e., ROP (glomerulosclerosis prone) and B6SJL (glomerulosclerosis resistant). ROP MC expressed 12-fold less MMP-9 mRNA. The MMP-9 promoter in ROP MC contained fewer d(CA) repeats, which was associated with lower MMP-9 expression and activity. Phorbol-12-myristate-13-acetate (3 to 60 ng/ml) increased MMP-9 expression in both MC types (3- to 4.5-fold), but the level in ROP MC never reached that in B6SLJ MC. Although reciprocal transfection of ROP and B6SJL MMP-9 promoter constructs into B6SJL and ROP cells revealed that the promoters were functional in both cell types, the B6SJL promoter was less responsive to phorbol-12-myristate-13-acetate stimulation when transfected into ROP MC, suggesting a role for other factors. In conclusion, the MMP-9 promoter exhibits a decreased number of d(CA) repeats in the sclerosis-prone strain. Because fewer d(CA) repeats associated with decreased MMP-9 expression in MC, it might be a genetic marker for glomerulosclerosis.
- Published
- 2002
- Full Text
- View/download PDF
20. Restoration of glomerular haemodynamics and renal injury independent of arterial hypertension in rats with subtotal renal ablation.
- Author
-
Herrera-Acosta J, Tapia E, Sánchez-Lozada LG, Franco M, Striker LJ, Striker GE, and Rodríguez IB
- Subjects
- Animals, Hypertension pathology, Mycophenolic Acid analogs & derivatives, Mycophenolic Acid pharmacology, Pentosan Sulfuric Polyester pharmacology, Rats, Systole, Glomerular Filtration Rate drug effects, Hypertension physiopathology, Kidney pathology, Nephrectomy
- Abstract
To study whether prevention of renal injury using the anti-inflammatory drugs pentosan polysulphate (PPS) and mycophenolate mofetil (MMF) is associated with improvement of glomerular haemodynamics, PPS and MMF were compared with losartan. The awake systolic blood pressure (SBP), proteinuria (Uprot) and micropuncture studies were performed 30 days after five-sixths nephrectomy in untreated rats and in rats treated with PPS (100 mg/kg per day), MMF (30 mg/kg per day) or losartan (30 mg/kg per day). In the rats receiving no treatment, there was a rise in SBP (to 180-200 mmHg) and in Uprot, which were prevented by losartan. In the PPS and MMF groups, the SBP was elevated but the Uprot did not increase. In the untreated rats the total glomerular filtration rate (GFR) decreased (-80%) and the single-nephron GFR (37-42%), plasma flow (67-127%) and glomerular pressure (10-15 mmHg) increased. These changes were prevented by PPS and MMF to the same extent as by losartan: the rise in single-nephron GFR and plasma flow were reduced by 50% and the glomerular pressure was normal. In rats receiving losartan, this was due to the fall in arterial pressure, whereas in PPS- and MMF-treated rats it was due to a rise in afferent resistance, indicating autoregulatory capacity. Total GFR was similar, despite the lower single-nephron GFR in treated groups, suggesting a larger proportion of functioning nephrons. Losartan, PPS and MMF significantly reduced glomerular sclerosis and tubular dilation and atrophy in association with a reduction in the lymphocyte and macrophage infiltrate. These results suggest an interaction between the haemodynamic and inflammatory changes that perpetuate each other during progression of renal injury. Renal protection provided by anti-inflammatory drugs is partially mediated by the prevention of glomerular haemodynamic alterations.
- Published
- 2002
21. Estrogen-related abnormalities in glomerulosclerosis-prone mice: reduced mesangial cell estrogen receptor expression and prosclerotic response to estrogens.
- Author
-
Potier M, Karl M, Zheng F, Elliot SJ, Striker GE, and Striker LJ
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Collagen Type IV metabolism, Estradiol pharmacology, Estrogen Receptor alpha, Estrogen Receptor beta, Estrogens pharmacology, Female, Gene Expression Regulation drug effects, Glomerular Mesangium cytology, Glomerular Mesangium drug effects, Glomerular Mesangium metabolism, Glomerulosclerosis, Focal Segmental pathology, Glomerulosclerosis, Focal Segmental prevention & control, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, RNA, Messenger drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, Estrogen genetics, Receptors, Estrogen metabolism, Species Specificity, Transcription, Genetic, Up-Regulation drug effects, Estrogens metabolism, Glomerulosclerosis, Focal Segmental metabolism
- Abstract
The development and progression of glomerulosclerosis (GS) is determined by the genetic background. The incidence of end-stage renal disease is increased in postmenopausal women, suggesting that estrogen deficiency may play a role in the accumulation of extracellular matrix by mesangial cells (MCs), which are primarily responsible for the synthesis and degradation of this matrix. Using mouse models that are prone or resistant to the development of GS, we compared the expression of estrogen receptor (ER)-alpha and ER-beta subtypes in GS-prone and GS-resistant glomeruli and isolated MCs, and examined the effects of estrogens on ER, collagen, and matrix metalloproteinase (MMP) expression in MCs. Glomeruli and MCs from GS-prone mice had decreased expression of ER-alpha and ER-beta subtypes and ER transcriptional activity was also decreased in their MCs. Importantly, although 17 beta-estradiol treatment resulted in decreased collagen accumulation and increased MMP-9 expression and activity in MCs from GS-resistant mice, there was, paradoxically, no effect on collagen accumulation and decreased MMP-9 expression and activity in MCs from GS-prone mice. Thus, GS susceptibility is associated with diminished ER expression in MCs. The renal protective effects of estrogens, including decreased collagen accumulation and increased MMP-9 expression, seem to be blunted in GS-prone MCs.
- Published
- 2002
- Full Text
- View/download PDF
22. Upregulation of type I collagen by TGF-beta in mesangial cells is blocked by PPARgamma activation.
- Author
-
Zheng F, Fornoni A, Elliot SJ, Guan Y, Breyer MD, Striker LJ, and Striker GE
- Subjects
- Animals, Biotransformation drug effects, Collagen Type I genetics, Diabetes Mellitus metabolism, Enzyme-Linked Immunosorbent Assay, Glomerular Mesangium drug effects, Glucose pharmacology, Mice, Mice, Inbred C57BL, Receptors, Cytoplasmic and Nuclear biosynthesis, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors biosynthesis, Up-Regulation drug effects, Collagen Type I biosynthesis, Glomerular Mesangium metabolism, Receptors, Cytoplasmic and Nuclear metabolism, Transcription Factors metabolism, Transforming Growth Factor beta pharmacology
- Abstract
We found that peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA was reduced by 77% in glomeruli of diabetic mice. Because mesangial cells play an important role in diabetic nephropathy, we examined regulation of type I collagen expression by PPARgamma and transforming growth factor-beta(1) (TGF-beta(1)) in mouse mesangial cells in the presence of 6 and 25 mM glucose. Mesangial cells contained functionally active PPARgamma. Exposure to 25 mM glucose resulted in reduced PPARgamma expression and transcriptional activity, accompanied by increased type I collagen expression. Restoration of PPARgamma activity to normal levels in cells cultured in 25 mM glucose, by transfection with a PPARgamma expression construct and treatment with the PPARgamma agonist troglitazone, returned type I collagen levels toward normal values. Activation of PPARgamma by troglitazone also decreased type I collagen mRNA and blocked TGF-beta(1)-mediated upregulation of type I collagen mRNA and protein. Moreover, PPARgamma activation suppressed basal and activated TGF-beta(1) responses in mesangial cells. This action was blocked by transfection of cells with a dominant-negative PPARgamma construct. In summary, PPARgamma suppresses the increased type I collagen mRNA and protein expression mediated by TGF-beta(1) in mesangial cells.
- Published
- 2002
- Full Text
- View/download PDF
23. Reversibility of glucose-induced changes in mesangial cell extracellular matrix depends on the genetic background.
- Author
-
Fornoni A, Striker LJ, Zheng F, and Striker GE
- Subjects
- Animals, Cells, Cultured, Collagen Type I genetics, Genes, Reporter drug effects, Genes, Reporter physiology, Luciferases genetics, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred Strains, RNA, Messenger metabolism, Species Specificity, Transforming Growth Factor beta genetics, Transforming Growth Factor beta pharmacology, Transforming Growth Factor beta physiology, Transforming Growth Factor beta1, Extracellular Matrix drug effects, Extracellular Matrix metabolism, Glomerular Mesangium metabolism, Glucose pharmacology
- Abstract
Adequate glycemic control protects most patients with diabetes from nephropathy, but a substantial fraction of patients develop progressive disease despite lowering glycemia. We isolated mesangial cells (MC) from the glomeruli of mouse strains that model these two outcomes in patients with diabetes, namely those that have the propensity (ROP) or resistance (B6) to develop progressive diabetic nephropathy. We determined the nature and reversibility of changes in selected extracellular matrix-related molecules after chronic exposure to elevated glucose concentration. MC were exposed to 25 mmol/l glucose for 5 weeks followed by 6 mmol/l glucose and 19 mmol/l mannitol for an additional 5 weeks. Matrix metalloproteinase-2 (MMP-2) and transforming growth factor-beta(1) (TGF-beta(1)) levels increased in B6 MC exposed to 25 mmol/l glucose but returned to baseline levels when the glucose concentration was reduced to 6 mmol/l. MMP-2 and TGF-beta(1) were higher in ROP MC at baseline and increased in response to 25 mmol/l glucose, but remained elevated when glucose concentration was reduced. Type I collagen expression and accumulation increased in a reversible manner in B6 MC exposed to 25 mmol/l glucose. However, type I collagen expression was higher in ROP MC at baseline and remained unaffected by changes in glucose concentration. Thus, 25 mmol/l glucose induced reversible changes in MMP-2, TGF-beta(1), and type I collagen in MC of sclerosis-resistant mice but not in MC from sclerosis-prone mice. Therefore, progressive diabetic nephropathy may be secondary to stable alterations in the phenotype of MC as a result of the interplay between the genetic background and elevated glucose concentrations.
- Published
- 2002
- Full Text
- View/download PDF
24. Autocrine activation of the IGF-I signaling pathway in mesangial cells isolated from diabetic NOD mice.
- Author
-
Tack I, Elliot SJ, Potier M, Rivera A, Striker GE, and Striker LJ
- Subjects
- Analysis of Variance, Animals, Cell Culture Techniques methods, Cell Line, Enzyme Inhibitors pharmacology, MAP Kinase Signaling System drug effects, MAP Kinase Signaling System physiology, Matrix Metalloproteinases metabolism, Mice, Mice, Inbred NOD, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases antagonists & inhibitors, Mitogen-Activated Protein Kinases metabolism, Phosphorylation, Receptor, IGF Type 1 physiology, Reference Values, Diabetes Mellitus, Type 1 physiopathology, Glomerular Mesangium physiopathology, Insulin-Like Growth Factor I physiology, Signal Transduction physiology
- Abstract
Mesangial cells isolated from NOD mice after the onset of diabetes have undergone a stable phenotypic change. This phenotype is characterized by increased expression of IGF-I and downregulation of collagen degradation, which is associated with decreased MMP-2 activity. Here, we investigated the IGF-I signaling pathway in mesangial cells isolated from NOD mice before (nondiabetic NOD mice [ND-NOD]) and after (diabetic NOD mice [D-NOD]) the onset of diabetes. We found that the IGF-I signaling pathway in D-NOD cells was activated by autocrine IGF-I. They had phosphorylation of the IGF-I receptor beta-subunit, phosphorylation of insulin receptor substrate (IRS)-1, and association of the p85 subunit (phosphatidylinositol 3-kinase [PI3K]) with the IGF-I receptor and IRS-1 in D-NOD cells in the basal state. This was also associated with increased phosphorylation of ERK2 in D-NOD mesangial cells. Inhibiting autocrine IGF-I from binding to its receptor using an IGF-I-neutralizing antibody or inhibiting IGF-I signaling pathways using a specific PI3K inhibitor or a specific mitogen-activated protein kinase/extracellular response kinase kinase inhibitor decreased phosphorylated ERKs in D-NOD cells. Importantly, this was associated with increased MMP-2 activity. The addition of exogenous IGF-I to ND-NOD activated signal transduction. Therefore, we conclude that the IGF-I signaling pathway is intact in both D-NOD and ND-NOD cells. However, the phenotypic change in D-NOD cells is associated with constitutive activation of the IGF-I signaling pathways, which may participate in the development and progression of diabetic glomerulosclerosis.
- Published
- 2002
- Full Text
- View/download PDF
25. Glomerulosclerosis is transmitted by bone marrow-derived mesangial cell progenitors.
- Author
-
Cornacchia F, Fornoni A, Plati AR, Thomas A, Wang Y, Inverardi L, Striker LJ, and Striker GE
- Subjects
- Animals, Female, Genotype, Hematopoiesis, Hypertrophy, Immune Tolerance, Matrix Metalloproteinase 2 genetics, Mice, Muscle, Smooth, Vascular cytology, Sclerosis, Bone Marrow Transplantation, Glomerular Mesangium cytology, Hematopoietic Stem Cell Transplantation, Kidney Glomerulus pathology
- Abstract
We found that ROP Os/+ (Os/+) mice had diffuse glomerulosclerosis and glomerular hypertrophy and that their mesangial cells (the vascular smooth muscle cells of the glomerulus) displayed an apparent sclerosing phenotype. Since mesangial cells are the major source of scar tissue in glomerulosclerosis, we postulated that the sclerosis phenotype was carried by mesangial cell progenitors and that this phenotype could be derived from the bone marrow (BM). Therefore, we transplanted BM from Os/+ mice into congenic ROP +/+ mice (+/+ mice), which have normal glomeruli. We found that glomeruli of +/+ recipients of Os/+ marrow contained the Os/+ genotype, were hypertrophied, and contained increased extracellular matrix. Clones of recipient glomerular mesangial cells with the donor genotype were found in all +/+ recipients that developed mesangial sclerosis and glomerular hypertrophy, whereas +/+ recipients of +/+ BM had normal glomeruli. Thus, the sclerotic (Os/+) or normal (+/+) genotype and phenotype were present in, and transmitted by, BM-derived progenitors. These data show that glomerular mesangial cell progenitors are derived from the BM and can deliver a disease phenotype to normal glomeruli. Glomerular lesions may therefore be perpetuated or aggravated, rather than resolved, by newly arriving progenitor cells exhibiting a disease phenotype.
- Published
- 2001
- Full Text
- View/download PDF
26. Pentosan polysulfate prevents glomerular hypertension and structural injury despite persisting hypertension in 5/6 nephrectomy rats.
- Author
-
Bobadilla NA, Tack I, Tapia E, Sánchez-Lozada LG, Santamaría J, Jiménez F, Striker LJ, Striker GE, and Herrera-Acosta J
- Subjects
- Animals, Blood Pressure, Hemodynamics drug effects, Hypertension etiology, Hypertension physiopathology, Kidney pathology, Kidney Glomerulus pathology, Male, Proteinuria urine, Rats, Rats, Wistar, Anticoagulants pharmacology, Hypertension pathology, Hypertension prevention & control, Kidney Glomerulus drug effects, Nephrectomy methods, Pentosan Sulfuric Polyester pharmacology
- Abstract
Five/six nephrectomy induces systemic and glomerular hypertension, glomerulosclerosis, proteinuria, and tubulointerstitial fibrosis. Polysulfate pentosan (PPS) decreases mesangial proliferation and extracellular matrix accumulation. The aim of this study was to determine whether PPS prevents glomerular hemodynamic changes and renal damage. Micropuncture studies were performed in three groups of eight male Wistar rats. Two groups included rats with 5/6 nephrectomy-one of which was treated with PPS in drinking water (100 mg/kg body wt) and the second of which received normal drinking water-and the third group consisted of normal rats that served as controls. Five/six nephrectomy produced systemic hypertension, a 50% reduction in GFR, and a 67% increase in single-nephron GFR due to elevated glomerular pressure and single-nephron plasma flow as well as proteinuria. Hypertension persisted in PPS-treated animals. Despite a similar reduction in GFR, PPS prevented the rise in single-nephron GFR, glomerular capillary hydrostatic pressure, and proteinuria. By morphometry, glomerular volume was increased by 46% and mesangial area by 94%. Fractional glomerular capillary area decreased by 24%. PPS prevented these changes. Tubular dilatation, epithelial cell atrophy, and increased interstitial area were largely prevented by PPS, as was the interstitial inflammatory infiltrate. These results suggest that the renal protection conferred by PPS was mediated both by prevention of glomerular hypertension as well as suppression of the inflammatory response. It was postulated that this was partly due to the preservation of a greater fraction of functional nephrons.
- Published
- 2001
- Full Text
- View/download PDF
27. N-phenacylthiazolium bromide decreases renal and increases urinary advanced glycation end products excretion without ameliorating diabetic nephropathy in C57BL/6 mice.
- Author
-
Schwedler SB, Verbeke P, Bakala H, Weiss MF, Vilar J, Depreux P, Fourmaintraux E, Striker LJ, and Striker GE
- Subjects
- Animals, Arginine pharmacology, Collagen metabolism, Diabetes Mellitus, Experimental urine, Diabetic Nephropathies pathology, Diabetic Nephropathies urine, Female, Glomerular Mesangium drug effects, Glomerular Mesangium pathology, Glycation End Products, Advanced urine, Kidney drug effects, Kidney pathology, Kidney Glomerulus drug effects, Kidney Glomerulus pathology, Lysine pharmacology, Mice, Mice, Inbred C57BL, Proteinuria, Arginine analogs & derivatives, Diabetes Mellitus, Experimental physiopathology, Diabetic Nephropathies physiopathology, Glycation End Products, Advanced metabolism, Kidney physiopathology, Lysine analogs & derivatives, Thiazoles pharmacology
- Abstract
Aims: Advanced glycation end products (AGE), which form from the non-enzymatic reaction of proteins and sugars, have been implicated in the pathogenesis of diabetic nephropathy. Recently, a compound [N-phenacylthiazolium bromide (PTB)] has been described which cleaves alpha,beta-dicarbonyl compounds. In the present study we used diabetic C57BL/6 mice to determine if PTB altered renal AGE levels and reduced diabetic glomerulosclerosis., Methods: Mice with stable hyperglycaemia induced by streptozotocin were given daily subcutaneous injections of either PTB (10 microg/g) or saline for 12 weeks. Renal-collagen bound AGE and urinary AGE-peptides were measured by ELISA using an anti-AGE-RNase antibody. Renal collagen-released Nepsilon(carboxymethyl)lysine (CML) and pentosidine were determined by high pressure liquid chromatography (HPLC). Glomerular lesions (volume and mesangial/total surface area) were evaluated by computer-assisted image analysis. We determined urinary protein/creatinine ratio as a functional parameter. AGE localization was examined by immunohistochemistry using the anti-AGE-RNase antibody., Results: Renal collagen-bound AGE were decreased and urinary AGE excretion was increased in PTB-treated diabetic mice. However, collagen-released CML and pentosidine were similar in both groups. Glomerular histology and morphometric analysis revealed also no differences between PTB-and saline-treated diabetic mice. The urinary protein/creatinine ratio was unaffected by PTB-treatment. AGE staining by anti-AGE-RNase antibody was present in Bowman's capsules, glomerular basement membranes and cortical tubules. It was decreased in all structures in PTB-treated diabetic mice., Conclusion: In summary, PTB decreased renal AGE accumulation but did not ameliorate glomerular lesions or proteinuria. Thus, cleavage of AGE by PTB is not sufficient to prevent development of diabetic nephropathy in C57BL/6 mice.
- Published
- 2001
- Full Text
- View/download PDF
28. Low-protein diet suppresses serum insulin-like growth factor-1 and decelerates the progression of growth hormone-induced glomerulosclerosis.
- Author
-
Doi SQ, Rasaiah S, Tack I, Mysore J, Kopchick JJ, Moore J, Hirszel P, Striker LJ, and Striker GE
- Subjects
- Animals, Cattle, Female, Glomerulosclerosis, Focal Segmental chemically induced, Growth Hormone, Mice, Mice, Transgenic, Diet, Protein-Restricted, Glomerulosclerosis, Focal Segmental diet therapy, Insulin-Like Growth Factor I metabolism
- Abstract
A low-protein (LP) diet has been associated with amelioration of renal function in glomerulosclerosis (GS). However, the mechanisms involved are still unclear. We have used a mouse transgenic for bovine growth hormone (GH), which develops progressive GS and exhibits consistently elevated levels of circulating GH and insulin-like growth factor (IGF)-1, to study the effect of dietary protein restriction. LP (6% protein) and normal-protein (NP, 20% protein) diets were maintained for 30 weeks in mice with established GS of mild/moderate degree. The degree of GS was markedly attenuated in LP compared to NP mice. Quantitative analysis revealed a significantly lower GS index (1.4 +/- 0.9 in LP vs. 2.8 +/- 0.8 in NP) and glomerular volume (0.8 x 10(6) +/- 0.1 x 10(6) microm(3) in LP vs. 1.2 x 10(6) +/- 0.1 x 10(6) microm(3) in NP) in mice with restricted protein intake. These morphologic changes were accompanied by a significant reduction in renal expression of alpha(1) type-IV collagen (2.4-fold) and tenascin (1.4-fold) in LP mice. Serum IGF-1 decreased by 40% and showed a significant correlation with alpha(1) type-IV collagen expression with the LP diet. The present finding supports the use of the LP diet to decelerate the progression of GS and furthermore suggests that one of the mechanisms involved in this process is the GH/IGF-1 regulation by protein intake., (Copyright 2001 S. Karger AG, Basel)
- Published
- 2001
- Full Text
- View/download PDF
29. Analysis of glomerulosclerosis and atherosclerosis in lecithin cholesterol acyltransferase-deficient mice.
- Author
-
Lambert G, Sakai N, Vaisman BL, Neufeld EB, Marteyn B, Chan CC, Paigen B, Lupia E, Thomas A, Striker LJ, Blanchette-Mackie J, Csako G, Brady JN, Costello R, Striker GE, Remaley AT, Brewer HB Jr, and Santamarina-Fojo S
- Subjects
- Animals, Arteriosclerosis physiopathology, Base Sequence, DNA Primers, Glomerulosclerosis, Focal Segmental physiopathology, Kidney physiopathology, Lipids blood, Lipoproteins blood, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Electron, Phosphatidylcholine-Sterol O-Acyltransferase blood, Phosphatidylcholine-Sterol O-Acyltransferase genetics, RNA, Messenger genetics, Arteriosclerosis enzymology, Glomerulosclerosis, Focal Segmental enzymology, Phosphatidylcholine-Sterol O-Acyltransferase physiology
- Abstract
To evaluate the biochemical and molecular mechanisms leading to glomerulosclerosis and the variable development of atherosclerosis in patients with familial lecithin cholesterol acyl transferase (LCAT) deficiency, we generated LCAT knockout (KO) mice and cross-bred them with apolipoprotein (apo) E KO, low density lipoprotein receptor (LDLr) KO, and cholesteryl ester transfer protein transgenic mice. LCAT-KO mice had normochromic normocytic anemia with increased reticulocyte and target cell counts as well as decreased red blood cell osmotic fragility. A subset of LCAT-KO mice accumulated lipoprotein X and developed proteinuria and glomerulosclerosis characterized by mesangial cell proliferation, sclerosis, lipid accumulation, and deposition of electron dense material throughout the glomeruli. LCAT deficiency reduced the plasma high density lipoprotein (HDL) cholesterol (-70 to -94%) and non-HDL cholesterol (-48 to -85%) levels in control, apoE-KO, LDLr-KO, and cholesteryl ester transfer protein-Tg mice. Transcriptome and Western blot analysis demonstrated up-regulation of hepatic LDLr and apoE expression in LCAT-KO mice. Despite decreased HDL, aortic atherosclerosis was significantly reduced (-35% to -99%) in all mouse models with LCAT deficiency. Our studies indicate (i) that the plasma levels of apoB containing lipoproteins rather than HDL may determine the atherogenic risk of patients with hypoalphalipoproteinemia due to LCAT deficiency and (ii) a potential etiological role for lipoproteins X in the development of glomerulosclerosis in LCAT deficiency. The availability of LCAT-KO mice characterized by lipid, hematologic, and renal abnormalities similar to familial LCAT deficiency patients will permit future evaluation of LCAT gene transfer as a possible treatment for glomerulosclerosis in LCAT-deficient states.
- Published
- 2001
- Full Text
- View/download PDF
30. Cyclosporin A affects extracellular matrix synthesis and degradation by mouse MC3T3-E1 osteoblasts in vitro.
- Author
-
Fornoni A, Cornacchia F, Howard GA, Roos BA, Striker GE, and Striker LJ
- Subjects
- Alkaline Phosphatase antagonists & inhibitors, Animals, Cell Death drug effects, Cell Division drug effects, Cell Line, Collagen antagonists & inhibitors, Collagen metabolism, Dose-Response Relationship, Drug, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Osteoblasts cytology, RNA, Messenger metabolism, Tissue Inhibitor of Metalloproteinase-2 genetics, Tissue Inhibitor of Metalloproteinase-2 metabolism, Cyclosporine pharmacology, Extracellular Matrix metabolism, Immunosuppressive Agents pharmacology, Osteoblasts metabolism
- Abstract
Background: Immunosuppressant therapy is thought to be a major contributor to post-transplant bone disease. Histological data and serum parameters suggest that Cyclosporin A (CsA) treatment causes osteopenia as a result of an altered bone turnover, but the pathogenic mechanisms of this process remain unclear. We investigate if CsA affects cell turnover and extracellular matrix (ECM) synthesis and degradation in MC3T3-E1 osteoblasts, as a surrogate model for in vivo events., Methods: Cells were exposed to increasing doses of CsA (0, 0.5, 1 and 5 microg/ml). Proliferation was evaluated by bromodeoxyuridine (BrdU) incorporation, viability by Trypan Blue exclusion and apoptosis by ELISA. Type I collagen was measured by ELISA and reverse transcription-polymerase chain reaction (RT-PCR), matrix metalloproteinases (MMP) by zymography and RT-PCR, and tissue inhibitors of MMP (TIMP) by reverse zymography., Results: CsA exposure for 48 h decreased osteoblast number in a dose-dependent manner in the absence of apoptosis or cytotoxicity. CsA at a dose of 5 microg/ml for 72 h caused decreased collagen type I mRNA expression and protein accumulation. While MMP-2 remained unaffected, MMP-9 activity increased. TIMP-1 activity was unaffected, while a dose-dependent increase of TIMP-2 was observed., Conclusions: These data suggest that CsA alters ECM synthesis and degradation in MC3T3-E1 osteoblasts by decreasing type I collagen production and increasing MMP-9 activity. The combination of increased MMP-9 with unchanged TIMP-1 activity could reduce the osteoid matrix available for mineralization. In addition, decreased proliferation could further reduce the number of cells synthesizing new osteoid matrix and thus contribute to the process of bone loss.
- Published
- 2001
- Full Text
- View/download PDF
31. Nitric oxide donor FK409 attenuates the development of neointimal hyperplasia in a rat aortic allograft model.
- Author
-
Fukada J, Schena S, Tack I, Ruiz P, Kurimoto Y, Pang M, Aitouche A, Abe T, Striker LJ, and Pham SM
- Subjects
- Animals, Aorta pathology, Hyperplasia prevention & control, Male, Rats, Rats, Inbred ACI, Rats, Inbred WF, Transplantation, Homologous, Aorta transplantation, Nitric Oxide Donors pharmacology, Nitro Compounds pharmacology, Tunica Intima pathology
- Published
- 2001
- Full Text
- View/download PDF
32. Expression and regulation of estrogen receptors in mesangial cells: influence on matrix metalloproteinase-9.
- Author
-
Potier M, Elliot SJ, Tack I, Lenz O, Striker GE, Striker LJ, and Karl M
- Subjects
- Animals, Cells, Cultured, Estrogen Antagonists pharmacology, Estrogen Receptor alpha, Estrogen Receptor beta, Estrogens pharmacology, Female, Humans, Mice, Mice, Inbred C57BL, Transcription, Genetic, Gene Expression Regulation, Glomerular Mesangium metabolism, Matrix Metalloproteinase 9 physiology, Receptors, Estrogen genetics
- Abstract
Diabetic glomerulosclerosis is characterized by the accumulation of extracellular matrix (ECM) in the mesangium. Estrogens seem to retard whereas estrogen deficiency seems to accelerate progressive glomerulosclerosis. Thus, mesangial cells (MC) may be a target for estrogens. Estrogen action is mediated via estrogen receptor (ER) subtypes ERalpha and ERbeta. Both ER subtypes were expressed in human and mouse MC. Using an estrogen-responsive reporter construct in transfection assays, it also was demonstrated that the nuclear ER were transcriptionally active. In the presence of 17beta-estradiol (E2; 10(-10) to 10(-8) M), there was a progressive increase in the mRNA levels of both ERalpha (approximately 1.8-fold and approximately 2.7-fold after 24 and 72 h, respectively) and ERbeta (approximately 1.3-fold and approximately 2.2-fold after 24 and 72 h, respectively). ERalpha protein levels increased approximately 2.5-fold after 24 h (10(-10) M, E2) and up to approximately 5.4-fold after 72 h (10(-9) M, E2). ERbeta protein levels increased approximately 2.1-fold in the presence of E(2) (10(-9) M) after 24 h. Thus, estrogens positively regulate the expression of the ER subtypes, thereby maintaining or increasing MC responsiveness to estrogens. Because diabetic glomerulosclerosis may be due partly to a decrease in ECM degradation, the effects of estrogens on matrix metalloproteinases (MMP) were studied. It was found that E2 (10(-10) to 10(-8) M) increased both MMP-9 mRNA and MMP-9 activity in MC. This may be an important mechanism by which estrogens influence ECM turnover and protect against progression of diabetic glomerulosclerosis.
- Published
- 2001
- Full Text
- View/download PDF
33. Hepatocyte growth factor, but not insulin-like growth factor I, protects podocytes against cyclosporin A-induced apoptosis.
- Author
-
Fornoni A, Li H, Foschi A, Striker GE, and Striker LJ
- Subjects
- Animals, Cells, Cultured, Dose-Response Relationship, Drug, Enzyme Inhibitors pharmacology, Fas Ligand Protein, Female, Flavonoids pharmacology, Kidney Glomerulus cytology, Kidney Glomerulus metabolism, MAP Kinase Kinase 1, Membrane Glycoproteins drug effects, Membrane Glycoproteins metabolism, Mice, Mitogen-Activated Protein Kinase Kinases antagonists & inhibitors, Mitogen-Activated Protein Kinase Kinases physiology, Phosphatidylinositol 3-Kinases physiology, Phosphoinositide-3 Kinase Inhibitors, Protein Serine-Threonine Kinases antagonists & inhibitors, Protein Serine-Threonine Kinases physiology, Proto-Oncogene Proteins c-bcl-2 drug effects, Proto-Oncogene Proteins c-bcl-2 metabolism, Time Factors, bcl-X Protein, fas Receptor drug effects, fas Receptor metabolism, Apoptosis drug effects, Cyclosporine pharmacology, Hepatocyte Growth Factor pharmacology, Insulin-Like Growth Factor I pharmacology, Kidney Glomerulus drug effects
- Abstract
Cyclosporin A (CsA) nephropathy is associated with altered expression of apoptosis regulatory genes such as Fas-ligand and Bcl-2 family members in the glomerular, tubulointerstitial, and vascular compartments. Both hepatocyte growth factor (HGF) and insulin-like growth factor (IGF-I) protect against apoptosis, and HGF specifically up-regulates Bcl-xL, a protein that regulates apoptosis. We investigated whether Bcl-xL and Fas/Fas-ligand were regulated by CsA in cultured podocytes and whether CsA-induced apoptosis was prevented by HGF or IGF-I. A murine podocyte cell line was treated with CsA in the presence or absence of HGF or IGF-I. Apoptosis was quantitated by ELISA and by flow cytometry; Bcl-xL, Fas, and Fas-ligand were measured by Western blotting. Inhibitors of MAP kinase/ERK kinase (MEK)-1 and of phosphatidylinositol 3'-kinase (PI3'-K) were used to determine the signaling pathways involved in Bcl-xL regulation. Apoptosis was induced by CsA in a dose- and time-dependent fashion. CsA also decreased Bcl-xL levels. HGF, but not IGF-I, prevented apoptosis and restored Bcl-xL levels. The regulation of Bcl-xL by HGF was mediated by the PI3'-K but not by the MEK-1 pathway. In summary, we showed that CsA induces apoptosis in podocytes. Apoptosis was prevented by pretreatment with HGF but not IGF-I. Decreased apoptosis appeared to be mediated by regulation of Bcl-xL via the PI3'-K pathway. Our data suggest that the effect of CsA on podocytes may contribute to the glomerular damage and that HGF could provide protection.
- Published
- 2001
- Full Text
- View/download PDF
34. Matrix accumulation in mesangial cells exposed to cyclosporine A requires a permissive genetic background.
- Author
-
Fornoni A, Lenz O, Tack I, Potier M, Elliot SJ, Striker LJ, and Striker GE
- Subjects
- Animals, Apoptosis, Collagen genetics, Disease Susceptibility, Extracellular Matrix drug effects, Glomerular Mesangium cytology, Glomerular Mesangium drug effects, Immunity, Innate, Matrix Metalloproteinase 2 genetics, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Reverse Transcriptase Polymerase Chain Reaction, Species Specificity, Tissue Inhibitor of Metalloproteinase-1 genetics, Tissue Inhibitor of Metalloproteinase-2 genetics, Transcription, Genetic, Transforming Growth Factor beta genetics, Transforming Growth Factor beta metabolism, Cyclosporine pharmacology, Extracellular Matrix physiology, Glomerular Mesangium physiology, Glomerulonephritis genetics, Matrix Metalloproteinase 2 metabolism, Tissue Inhibitor of Metalloproteinase-1 metabolism, Tissue Inhibitor of Metalloproteinase-2 metabolism
- Abstract
Background: Chronic nephrotoxicity is an important adverse effect of cyclosporine A (CsA) therapy. Tubulo-interstitial lesions and arteriolopathy are common histologic findings. Glomerular lesions are also described, but they are of variable severity. The aim of our study is to determine whether CsA has a direct effect on mesangial cells and whether the cellular response depends on the genetic background., Methods: We studied mesangial cells isolated from mice susceptible (ROP/Le-+Es1(b)+Es1(a), ROP) and resistant to glomerulosclerosis (B6SJLF1, C57). We previously showed that sclerosis-prone and sclerosis-resistant phenotypes are maintained in vitro. We examined whether CsA exposure directly affected extracellular matrix turnover in mesangial cells and whether the response is determined by the genetic background. Extracellular matrix synthesis and degradation were studied by proline incorporation, ELISA, reverse transcription-polymerase chain reaction, zymography, and reverse zymography. We chose a CsA dose that induced neither cytotoxicity nor apoptosis (1 microg/ml)., Results: At the dose of 1 microg/ml total collagen accumulation was increased in ROP but not in C57 cells. Matrix metalloproteinase (MMP)-2 activity and mRNA levels were selectively decreased in ROP cells. CsA exposure did not affect tissue inhibitors of MMP (TIMP)-1 and -2 activity or TGF-beta1 mRNA expression and protein synthesis in either cell line., Conclusion: CsA increases total collagen accumulation in mesangial cells from sclerosis-prone mice by decreasing MMP-2 activity, but does not affect cells from sclerosis-resistant mice. Thus, CsA directly affects mesangial cells, but only those with a permissive genetic background for glomerulosclerosis.
- Published
- 2000
- Full Text
- View/download PDF
35. Growth hormone increases inducible nitric oxide synthase expression in mesangial cells.
- Author
-
Doi SQ, Jacot TA, Sellitti DF, Hirszel P, Hirata MH, Striker GE, and Striker LJ
- Subjects
- Animals, Cells, Cultured, Culture Media, Conditioned chemistry, Dose-Response Relationship, Drug, Glomerular Mesangium cytology, Glomerular Mesangium drug effects, Lipopolysaccharides pharmacology, Mice, Nitric Oxide Synthase genetics, Nitric Oxide Synthase Type II, Nitrites analysis, Ornithine Decarboxylase metabolism, Ornithine-Oxo-Acid Transaminase metabolism, RNA, Messenger metabolism, omega-N-Methylarginine pharmacology, Glomerular Mesangium enzymology, Growth Hormone pharmacology, Nitric Oxide Synthase metabolism
- Abstract
Mice transgenic for bovine growth hormone (GH) develop progressive glomerulosclerosis. However, the proximal signaling events that lead to increased matrix deposition in this pathologic condition are still unclear. Components of the L-arginine metabolic pathway, especially inducible nitric oxide (NO) synthase (iNOS), ornithine aminotransferase (OAT), and ornithine decarboxylase (ODC), have been associated with glomerular scarring. In this study, mesangial cells were treated with GH, and the expression of iNOS, ODC, and OAT was determined using reverse transcription-PCR. In addition, nitrite accumulation in the conditioned media of mesangial cell cultures was measured in the presence or absence of GH. The findings revealed that GH increased iNOS transcript levels in a dose-dependent manner, with the highest levels being attained at GH concentrations of 20 to 50 ng/ml. The GH-induced increase in iNOS transcript levels was accompanied by a significant increase in nitrite concentrations in conditioned media, which was blocked by the addition of L-N(G)-monomethylarginine. The effect of GH (50 ng/ml) in eliciting nitrite production was as potent as that of bacterial lipopolysaccharide (10 microg/ml). The expression of OAT and ODC, in contrast, was not altered at any of the GH concentrations tested. GH receptor mRNA was also expressed by mesangial cells, independently of the GH concentration present in the cell culture medium. These data indicate that GH may interact with its receptor to regulate the L-arginine/NO pathway in mesangial cells, by directly modulating iNOS expression and NO production, without altering the arginase/OAT/ODC pathway.
- Published
- 2000
- Full Text
- View/download PDF
36. Pentosan polysulfate decreases proliferation and extracellular matrix deposition by vascular smooth muscle cells isolated from failed hemodialysis access grafts.
- Author
-
Elliot SJ, Striker LJ, Connor E, Stetler-Stevenson W, McQuinn WC, Blagg CR, and Striker GE
- Subjects
- Cell Division drug effects, Collagen metabolism, Enzyme-Linked Immunosorbent Assay, Humans, Matrix Metalloproteinase 9 metabolism, Muscle, Smooth, Vascular cytology, Polytetrafluoroethylene, Reoperation, Tissue Inhibitor of Metalloproteinase-3 metabolism, Blood Vessel Prosthesis, Extracellular Matrix drug effects, Graft Occlusion, Vascular prevention & control, Muscle, Smooth, Vascular drug effects, Pentosan Sulfuric Polyester pharmacology, Renal Dialysis
- Abstract
Background: Vascular access failure is a major cause of morbidity, and increased costs in patients undergoing maintenance hemodialysis. Stenosis, the most common underlying cause of loss of patency in failed grafts, appears to be caused by an obstructing mass of tissue containing proliferating smooth muscle cells and their associated extracellular matrix., Methods: To determine whether this process was amenable to pharmacologic intervention and/or prevention, we obtained samples of the material occluding vascular accesses from 7 patients undergoing revision surgery in order to characterize the cells contributing to the stenosis. In all 7 patients the outgrowth contained predominantly smooth muscle-like cells admixed with fibroblasts, which produced a large amount of type IV and type I collagen., Results: Treatment with pentosan polysulfate inhibited cell proliferation and significantly reduced the accumulation of types I and type IV collagens. This was associated with increase in metalloproteinase-9 (MMP-9) and a shift of tissue inhibitor of metalloproteinase-3 (TIMP-3) from the cell layer into the medium., Conclusion: These data suggest that pentosan polysulfate (PPS) may have a favorable effect in patients with a polytetrafluoroethylene (PFTE) graft by decreasing cell proliferation and collagen deposition.
- Published
- 2000
37. FK409, a spontaneous nitric oxide releaser, attenuates allograft vasculopathy in a rat aortic transplant model.
- Author
-
Fukada J, Schena S, Tack I, Ruiz P, Kurimoto Y, Pang M, Aitouche A, Abe T, Striker LJ, and Pham SM
- Subjects
- Animals, Aorta pathology, Apoptosis drug effects, Bromodeoxyuridine metabolism, Cyclosporine pharmacology, Immunohistochemistry, Male, Muscle, Smooth, Vascular metabolism, Muscle, Smooth, Vascular pathology, Rats, Rats, Inbred ACI, Rats, Inbred WF, Transplantation, Homologous, Aorta transplantation, Muscle, Smooth, Vascular drug effects, Nitric Oxide Donors pharmacology, Nitro Compounds pharmacology
- Abstract
Although systemic administration of NO donors has been shown to attenuate the development of neointimal hyperplasia in the balloon injury model, this strategy has not been tested in a model of allograft vasculopathy. In this study, we investigated the effect of FK409, a spontaneous NO releaser, on the development of allograft vasculopathy, using a rat aortic transplant model. Thoracic aortas from ACI rats were transplanted heterotopically into the abdominal aorta of Wistar-Furth rats. Postoperatively, recipients received FK409 orally every 8 hours from the day of transplantation to the time of euthanization. Morphometric and immunohistochemical analyses were performed on the aortic grafts 8 weeks after transplantation. Control allografts showed severe neointimal hyperplasia, which consists mainly of alpha-actin-containing vascular smooth muscle cells. The FK409-treated allografts showed a dose-dependent reduction (statistically significant compared with the control) in the neointimal thickness as the dose increased from 1 to 10 mg/kg (thrice per day). However, there was no significant difference in the neointimal thickness between groups treated with 10 and with 20 mg/kg. FK409 treatment (10 mg/kg) caused a significant decrease in DNA synthesis (5-bromo-2-deoxyuridine [BrdU] uptake), an increase in DNA fragmentation (terminal deoxynucleotidyltransferase-mediated uridine nick-end labeling [TUNEL]), and upregulation of Fas expression, in the neointimal vascular smooth muscle cells. These data suggest that FK409 attenuates the allograft vasculopathy in a rat aortic transplant model.
- Published
- 2000
- Full Text
- View/download PDF
38. Glomerulosclerosis in mice transgenic for human insulin-like growth factor-binding protein-1.
- Author
-
Doublier S, Seurin D, Fouqueray B, Verpont MC, Callard P, Striker LJ, Striker GE, Binoux M, and Baud L
- Subjects
- Animals, Blood Pressure, Body Weight, Creatinine blood, Creatinine urine, Disease Susceptibility, Glomerulosclerosis, Focal Segmental blood, Glomerulosclerosis, Focal Segmental physiopathology, Growth Hormone blood, Humans, Insulin-Like Growth Factor Binding Protein 1 genetics, Kidney pathology, Kidney Glomerulus pathology, Mice, Mice, Transgenic genetics, Organ Size, Proteinuria urine, Urea blood, Glomerulosclerosis, Focal Segmental pathology, Insulin-Like Growth Factor Binding Protein 1 physiology
- Abstract
Background: The growth hormone (GH)/insulin-like growth factor (IGF) system is thought to participate in the glomerulosclerosis process. Because IGF-binding proteins (IGFBPs) modulate IGF actions and hence GH secretion, this study assessed whether mice transgenic for human IGFBP-1 have altered susceptibility to glomerulosclerosis., Methods: A line of transgenic mice that express human IGFBP-1 mRNA in the liver under the control of the alpha1-antitrypsin promoter has been obtained, and morphological changes in the kidney tissue were assessed. Glomerulosclerosis was identified using light microscopy, light microscopic morphometry, and electron microscopy. Extracellular matrix components were analyzed by immunohistochemistry., Results: There was a marked increase in mesangial extracellular matrix area in homozygous transgenic mice at three months of age as compared with heterozygous transgenic mice and nontransgenic littermates. These changes were not associated with alterations in glomerular volume or cellularity. The expansion of extracellular matrix area was related to a marked increase in laminin and type IV collagen and to the appearance of type I collagen., Conclusions: These observations indicate that the enhanced expression of IGFBP-1 may result in the development of glomerulosclerosis without glomerular hypertrophy. The changes are potentially related to a decrease in IGF-I availability and/or to an IGF-I-independent role of IGFBP-1.
- Published
- 2000
- Full Text
- View/download PDF
39. Protection against diabetes-induced nephropathy in growth hormone receptor/binding protein gene-disrupted mice.
- Author
-
Bellush LL, Doublier S, Holland AN, Striker LJ, Striker GE, and Kopchick JJ
- Subjects
- Animals, Blood Glucose metabolism, Body Weight physiology, Diabetic Neuropathies physiopathology, Female, Glomerular Mesangium pathology, Kidney pathology, Mice, Mice, Knockout, Organ Size physiology, Signal Transduction genetics, Signal Transduction physiology, Diabetic Neuropathies genetics, Diabetic Neuropathies pathology, Receptors, Somatotropin genetics
- Abstract
To further investigate the role of GH in diabetic nephropathy, experimental diabetes was induced with streptozotocin (STZ) in mice in which the GH receptor/binding protein gene was disrupted. Body weight, blood glucose, and renal histology and morphometry were studied 10 weeks after diabetes induction in wild-type (+/+) mice and in mice heterozygous (+/-) and homozygous (-/-) for the disruption. Equivalent levels of hyperglycemia developed in all diabetic groups. Normal weight gain was absent in +/+ and +/- diabetic groups, and -/- diabetics lost weight during the study. Diabetic +/+ and +/- groups both showed evidence of glomerulosclerosis, increases in glomerular volume, and increases in the ratio of mesangial area to total glomerular area, whereas diabetic -/- mice showed none of these pathological changes. These results extend our previous findings of protection against diabetes-associated kidney damage in transgenic mice expressing a GH antagonist. Taken together, the results argue for an important role of GH in the development of diabetes induced end-organ damage.
- Published
- 2000
- Full Text
- View/download PDF
40. Pentosan polysulfate treatment reduces cyclosporine-induced nephropathy in salt-depleted rats.
- Author
-
Schwedler SB, Bobadilla N, Striker LJ, Vaamonde CA, Herrera-Acosta J, and Striker GE
- Subjects
- Animals, Arterioles drug effects, Arterioles pathology, Creatinine metabolism, Diet, Sodium-Restricted, Kidney pathology, Kidney physiology, Kidney Cortex enzymology, Kidney Glomerulus drug effects, Kidney Glomerulus pathology, Male, Mice, Nitric Oxide Synthase genetics, Nitric Oxide Synthase Type I, Nitric Oxide Synthase Type II, Nitric Oxide Synthase Type III, RNA, Messenger genetics, Rats, Rats, Sprague-Dawley, Renal Circulation drug effects, Transcription, Genetic drug effects, Cyclosporine toxicity, Kidney drug effects, Pentosan Sulfuric Polyester pharmacology
- Abstract
Background: Long-term cyclosporine (CsA) treatment leads to a decreased glomerular filtration rate, hyalinosis of afferent arterioles, and striped cortical tubulo-interstitial fibrosis. We showed previously that pentosan polysulfate (SP54) prevented the development of microvascular and interstitial lesions in mouse models of progressive glomerulosclerosis. In this study, we examined the effect of pentosan polysulfate on the development of CsA nephropathy., Methods: Pair-fed Sprague-Dawley rats were fed a low-sodium (0.03%) diet and received CsA (15 mg/kg, subcutaneously, in olive oil)/5% glucose, pentosan polysulfate (10 mg/kg, subcutaneously in 5% glucose) plus CsA, olive oil/pentosan polysulfate, or olive oil/5% glucose for 30 days. Creatinine clearance (CrCl) was determined at three time points. Afferent arteriolar lesions, glomerular volume, and tubulo-interstitial lesions were quantitated. RNA was extracted from cortex., Results: Severe lesions were found in the CsA group. A reduction in the number of affected arterioles (32%) and the degree of chronic tubulo-interstitial lesions (44%) was found in pentosan polysulfate/CsA-treated rats. A 20% decrease in glomerular volume was found in CsA rats, but not in pentosan polysulfate/CsA-treated rats. Pentosan polysulfate treatment did not prevent the CsA-induced decrease in CrCl (approximately 30%) at 4 weeks. CsA did not affect cortical endothelial or neuronal nitric-oxide synthase or mRNA levels, but there was small increase in neuronal nitric-oxide synthase mRNA levels in the pentosan polysulfate/CsA-treated group., Conclusions: Pentosan polysulfate reduced structural renal lesions in CsA-treated, salt-depleted Sprague-Dawley rats.
- Published
- 1999
- Full Text
- View/download PDF
41. Nephrotoxin exposure in utero reduces glomerular number in sclerosis-prone but not sclerosis-resistant mice.
- Author
-
Schwedler SB, Gilbert T, Moreau E, Striker LJ, Merlet-Bénichou C, and Striker GE
- Subjects
- Animals, Female, Gentamicins toxicity, Kidney Glomerulus drug effects, Mice, Mice, Inbred C57BL, Pregnancy, Sclerosis, Fetus drug effects, Kidney drug effects, Kidney Glomerulus embryology, Kidney Glomerulus pathology
- Abstract
Background: We have previously found that nephron number was not fixed, that is, there was a direct correlation between low birth weight and decreased nephron number in infants. In sclerosis-prone rats, we found that gentamicin exposure in utero induced a reduction in glomerular number and aggravated glomerulosclerosis in adults. In mice, we found that an inborn 50% reduction in nephron number, caused by the Os mutation, was associated with glomerulosclerosis in sclerosis-prone (ROP+/+) mice, but not in sclerosis-resistant (C57BL/6J) mice. Because the genetic background determined the response to decreased nephron number, we asked whether the susceptibility changes in glomerular number and glomerulosclerosis were linked., Methods: Gentamicin was administered before and after the onset of fetal nephrogenesis. (1) Prior to the onset of nephrogenesis, two groups of pregnant mice were treated from embryonic day (E) E8 to E12. In group A, early glomerular development was studied by placing ureteric ridges removed on E12 in vitro for four days, following which the ureteric bud branches and glomeruli were counted using lectin staining. In group B, nephron number was determined in spontaneously delivered 14-day-old (14PN) pups by counting glomeruli. (2) After the onset of nephrogenesis, to determine the direct effects of gentamicin on nephron induction, ureteric ridges were placed in organ culture at E12 of normal gestation, in the presence or absence of gentamicin. The number of glomeruli and ureteric bud branches were counted after six days in culture., Results: A decrease in glomerular number and ureteric bud branches was observed in sclerosis-prone (ROP+/+) mice, irrespective of whether gentamicin was administered prior to or after the onset of nephrogenesis. Glomerular number and ureteric bud branching were not decreased by gentamicin in sclerosis-resistant (C57BL/6) mice., Conclusions: These data provide evidence that there is a positive correlation between the susceptibility to glomerulosclerosis in adulthood and a reduction in nephron number in utero. Thus, exposure to nephrotoxins in utero compounds the risk of renal failure as an adult in sclerosis-prone individuals.
- Published
- 1999
- Full Text
- View/download PDF
42. IGF-1 decreases collagen degradation in diabetic NOD mesangial cells: implications for diabetic nephropathy.
- Author
-
Lupia E, Elliot SJ, Lenz O, Zheng F, Hattori M, Striker GE, and Striker LJ
- Subjects
- Animals, Cells, Cultured, Collagen biosynthesis, Collagen genetics, Collagenases genetics, Collagenases metabolism, Diabetes Mellitus, Type 1 genetics, Diabetic Nephropathies etiology, Female, Gelatinases genetics, Gelatinases metabolism, Glomerular Mesangium pathology, Glomerulosclerosis, Focal Segmental etiology, Humans, Insulin-Like Growth Factor I metabolism, Laminin genetics, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Metalloendopeptidases genetics, Metalloendopeptidases metabolism, Mice, Mice, Inbred NOD genetics, RNA, Messenger metabolism, Tissue Inhibitor of Metalloproteinases metabolism, Collagen metabolism, Diabetes Mellitus, Type 1 metabolism, Glomerular Mesangium metabolism, Insulin-Like Growth Factor I pharmacology, Mice, Inbred NOD metabolism
- Abstract
Nonobese diabetic (NOD) mice develop glomerulosclerosis shortly after the onset of diabetes. We showed that mesangial cells (MCs) from diabetic mice exhibited a stable phenotypic switch, consisting of both increased IGF-1 synthesis and proliferation (Elliot SJ, Striker LJ, Hattori M, Yang CW, He CJ, Peten EP, Striker GE: Mesangial cells from diabetic NOD mice constitutively secrete increased amounts of insulin-like growth factor-I. Endocrinology 133:1783-1788, 1993). Because the extracellular matrix (ECM) accumulation in diabetic glomerulosclerosis may be partly due to decreased degradation, we examined the effect of excess IGF-1 on collagen turnover and the activity of metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase (TIMPs) in diabetic and nondiabetic NOD-MC. Total collagen degradation was reduced by 58 +/- 18% in diabetic NOD-MCs, which correlated with a constitutive decrease in MMP-2 activity and mRNA levels, and nearly undetectable MMP-9 activity and mRNA. TIMP levels were slightly decreased in diabetic NOD-MC. The addition of recombinant IGF-1 to nondiabetic NOD-MC resulted in a decrease in MMP-2 and TIMP activity. Furthermore, treatment of diabetic NOD-MC with a neutralizing antibody against IGF-1 increased the latent form, and restored the active form, of MMP-2. In conclusion, the excessive production of IGF-1 contributes to the altered ECM turnover in diabetic NOD-MC, largely through a reduction of MMP-2 activity. These data suggest that IGF-1 could be a major contributor to the development of diabetic glomerulosclerosis.
- Published
- 1999
- Full Text
- View/download PDF
43. Nature and severity of the glomerular response to nephron reduction is strain-dependent in mice.
- Author
-
Esposito C, He CJ, Striker GE, Zalups RK, and Striker LJ
- Subjects
- Animals, Body Weight physiology, Female, Glomerulosclerosis, Focal Segmental genetics, Kidney Glomerulus metabolism, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Organ Size physiology, Postoperative Period, RNA, Messenger metabolism, Species Specificity, Time Factors, Kidney Glomerulus pathology, Nephrectomy methods
- Abstract
Nephron reduction is an important factor in the development of glomerulosclerosis. In a study of the oligosyndactyly (Os) mutation that causes a congenital 50% reduction in nephron number, we previously found that ROP Os/+ mice developed glomerulosclerosis whereas C57B1/6J Os/+ mice did not. We concluded that the predisposition to glomerulosclerosis depended largely on the genetic background, the ROP being sclerosis-prone whereas the C57 strain was sclerosis-resistant. In the current experiments we asked whether the intensity of the sclerotic response to nephron reduction in the ROP strain was related to the time at which it occurred, ie, a pre- or post-natal event. We also determined whether the absence of lesions in C57 Os/+ mice was caused by a higher threshold for the induction of a sclerotic response in C57 mice. We further examined the relationship between glomerular hypertrophy and sclerosis. C57 +/+, C57 Os/+, ROP +/+, and ROP Os/+ mice were uninephrectomized (NX) at age 10 weeks and followed for 8 weeks. We found no sclerotic changes in NX C57 +/+ and C57 Os/+ mice, despite a 75% reduction in nephron number in the latter. In contrast, both NX ROP +/+ and NX ROP Os/+ mice had glomerulosclerosis, which was more severe in the NX ROP Os/+ mice. Examination of extracellular matrix synthesis and degradation at the mRNA level revealed that synthesis exceeded degradation in ROP Os/+ mice. The lesions in NX ROP +/+ were less severe than in sham-operated ROP/Os mice, suggesting that the timing of nephron reduction affected the amplitude of the sclerotic response in this strain. Following NX, an increase in glomerular volume was found in C57 +/+, ROP +/+, and ROP Os/+ mice. However, NX did not lead to a further increase in glomerular volume in C57 Os/+ mice. We make three conclusions: 1) sclerosis was more severe in the ROP strain when nephron reduction occurred in utero; 2) the absence of glomerulosclerosis in C57 mice was not related to a higher threshold for a sclerosis response in this strain; and 3) whereas glomerular size continued to increase as nephron number decreased in ROP mice, it reached a plateau in C57 mice.
- Published
- 1999
- Full Text
- View/download PDF
44. Pentosan polysulfate decreases proliferation and net extracellular matrix production in mouse mesangial cells.
- Author
-
Elliot SJ, Striker LJ, Stetler-Stevenson WG, Jacot TA, and Striker GE
- Subjects
- Animals, Cell Division drug effects, Cells, Cultured, Collagen analysis, Collagen biosynthesis, Dose-Response Relationship, Drug, Gelatinases analysis, Matrix Metalloproteinase 2, Metalloendopeptidases analysis, Mice, Mice, Inbred C57BL, Tissue Inhibitor of Metalloproteinases analysis, Extracellular Matrix drug effects, Glomerular Mesangium drug effects, Pentosan Sulfuric Polyester pharmacology
- Abstract
Glomerulosclerosis is characterized by extracellular matrix accumulation and is often associated with mesangial cell proliferation. Heparin-like molecules have been shown to decrease glomerulosclerosis in vivo, although their cellular site and mechanism of action is still unclear. In this study, a line of glomerular mesangial cells derived from normal mice was used to determine whether pentosan polysulfate (PPS) inhibited proliferation and altered extracellular matrix turnover. Cells treated with PPS showed a decrease in cell number beginning 24 h after treatment, which was maintained for 5 d. For matrix accumulation and degradation studies, cells were treated for 5 d and collagen types I and IV protein were measured by enzyme-linked immunosorbent assay as well as matrix metalloproteinases (MMP) measured by zymography. Collagen types 1 and type IV were significantly decreased in the media (P < 0.0001) and cell layer (P < 0.005) after treatment with PPS but not after treatment with heparin. By zymography, MMP-2 was significantly increased after treatment with PPS (P < 0.001) and heparin (P < 0.05). PPS and heparin also decreased MMP-9 (P < 0.001) after treatment. Reverse zymography showed the presence of tissue inhibitors of metalloproteinases (TIMP)-1 and -2 in control mesangial cells. Treatment with PPS and heparin increased TIMP-1. In addition, TIMP-3 was found in the medium of treated but not control cells. In conclusion, PPS alters extracellular matrix turnover through the induction of MMP-2 and alterations in the TIMP profile and may be useful in decreasing progressive glomerulosclerosis.
- Published
- 1999
- Full Text
- View/download PDF
45. Strain differences rather than hyperglycemia determine the severity of glomerulosclerosis in mice.
- Author
-
Zheng F, Striker GE, Esposito C, Lupia E, and Striker LJ
- Subjects
- Animals, Cell Count, Collagen genetics, Diabetes Mellitus, Experimental blood, Diabetes Mellitus, Experimental pathology, Diabetes Mellitus, Experimental physiopathology, Diabetic Nephropathies pathology, Glomerulosclerosis, Focal Segmental pathology, Laminin genetics, Mice, RNA, Messenger metabolism, Tenascin genetics, Diabetic Nephropathies blood, Diabetic Nephropathies physiopathology, Glomerulosclerosis, Focal Segmental blood, Glomerulosclerosis, Focal Segmental physiopathology, Hyperglycemia complications, Mice, Inbred Strains physiology
- Abstract
Background: We reported that ROP, but not C57, mice were prone to glomerulosclerosis (GS) after nephron reduction (J Clin Invest 97:1242, 1996)., Methods: In this study, we induced diabetes in ROP and C57 mice to determine if the glomerulosclerotic response was stimulus specific. We used the oligosyndactyly mutation (Os), to produce a congenital 50% reduction in nephron number. Stable hyperglycemia was induced by streptozotocin and mice were maintained for 12 weeks without insulin treatment., Results: Glomerular hypertrophy occurred in diabetic ROP +/+ and C57 +/+ mice, but glomeruli of diabetic ROP +/+ mice had 1.92-fold higher laminin B1 and 1.5-fold higher tenascin mRNA levels than diabetic C57 +/+ mice. Diabetic ROP Os/+ mice had severe glomerulosclerosis with arteriolar and tubulointerstitial lesions while there was only moderate mesangial sclerosis in diabetic C57 Os/+ mice. Glomerular size was increased in all non-diabetic Os/+ mice. It was further increased in diabetic ROP Os/+ mice, but not in diabetic C57 Os/+ mice. Glomerular mRNA levels were higher in diabetic ROP OS/+ than in diabetic C57 OS/+ mice [alpha 1 (i.v.) collagen 3.2-fold, laminin B1 2.1-fold, and tenascin 1.6-fold]., Conclusion: Overall, our data further support the hypothesis that the susceptibility to glomerulosclerosis is inherited, and suggest that hyperglycemia serves principally as a triggering event in the development of diabetic nephropathy. Since the acceleration of diabetic nephropathy by nephron reduction was also largely strain dependent, it appears that the propensity to glomerulosclerosis is a general renal response and is not stimulus specific.
- Published
- 1998
- Full Text
- View/download PDF
46. Glomerular endothelial cells synthesize collagens but little gelatinase A and B.
- Author
-
Lenz O, Striker LJ, Jacot TA, Elliot SJ, Killen PD, and Striker GE
- Subjects
- Animals, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Kidney Glomerulus cytology, Kidney Glomerulus drug effects, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Mice, Mice, Inbred Strains, Reverse Transcriptase Polymerase Chain Reaction, Transforming Growth Factor beta pharmacology, Collagen biosynthesis, Collagenases biosynthesis, Gelatinases biosynthesis, Kidney Glomerulus metabolism, Metalloendopeptidases biosynthesis
- Abstract
Mesangial sclerosis is a major feature of progressive renal disease. The mesangium contains mesangial cells and is bounded by the peripheral glomerular basement membrane and endothelial cells. Mesangial cells synthesize and degrade extracellular matrix. Whereas both mesangial and endothelial cells synthesize extracellular matrix components, the degradative pathway, well studied in the former, has not been investigated in endothelial cells. This study examines lines of all three glomerular cell types derived from female B6SJLF1/J mice, as well as mRNA levels for collagens alpha1(I), alpha1(IV), alpha3 (IV), alpha5 (IV), and alpha1 (VI), laminin, tenascin, matrix metalloproteinase-2 (MMP-2), and MMP-9. Type I and IV collagen synthesis was confirmed by enzyme-linked immunosorbent assay. MMP-2 and MMP-9 enzyme activity was measured by zymography. It was found that glomerular endothelial cells are a significant source of collagens, laminin, and tenascin. However, they express only low levels of MMP-2 and no detectable MMP-9. Stimulation with exogenous transforming growth factor-beta1 leads to a significant increase in collagen I, tissue inhibitors of metalloproteinase-1, and MMP-9 in conditioned media. These data suggest that glomerular endothelial cells may play an active role in extracellular matrix remodeling in glomerular disease.
- Published
- 1998
- Full Text
- View/download PDF
47. Linkage disequilibrium mapping reveals suppressed recombination at the Os locus.
- Author
-
Lenz O, Teichmann U, Langers A, Striker LJ, Striker GE, and Pavan WJ
- Subjects
- Animals, Crosses, Genetic, Crossing Over, Genetic, Female, Genes, Recessive, Genotype, Male, Mice, Mice, Inbred C3H genetics, Mice, Mutant Strains genetics, Probability, Chromosome Mapping, Linkage Disequilibrium, Recombination, Genetic, Syndactyly genetics
- Published
- 1998
- Full Text
- View/download PDF
48. Nephron reduction in man--lessons from the Os mouse.
- Author
-
Striker LJ
- Subjects
- AIDS-Associated Nephropathy genetics, AIDS-Associated Nephropathy pathology, Animals, Diabetic Nephropathies genetics, Diabetic Nephropathies pathology, Disease Models, Animal, Glomerulonephritis genetics, HIV-1, Humans, Male, Mice, Phenotype, Syndactyly genetics, Syndactyly pathology, Glomerulonephritis pathology, Mice, Mutant Strains genetics, Nephrons abnormalities, Nephrons pathology
- Published
- 1998
- Full Text
- View/download PDF
49. Glomerulosclerosis, arteriosclerosis, and vascular graft stenosis: treatment with oral heparinoids.
- Author
-
Striker GE, Lupia E, Elliot S, Zheng F, McQuinn C, Blagg C, Selim S, Vilar J, and Striker LJ
- Subjects
- Animals, Arteriosclerosis etiology, Arteriosclerosis pathology, Cattle, Glomerulosclerosis, Focal Segmental etiology, Glomerulosclerosis, Focal Segmental pathology, Graft Occlusion, Vascular pathology, Humans, Mice, Mice, Transgenic, Rabbits, Anticoagulants therapeutic use, Arteriosclerosis drug therapy, Glomerulosclerosis, Focal Segmental drug therapy, Graft Occlusion, Vascular drug therapy, Heparin therapeutic use
- Abstract
At present there is no known treatment for established glomerulosclerosis or atherosclerosis. Since the principal lesion in glomerulosclerosis involves mesangial cells, a vascular smooth muscle cell, we searched for new therapeutic approaches affecting vascular smooth muscle function, especially with respect to modifying the turnover of extracellular matrix. We used mice transgenic for bovine growth hormone (bGH), since these mice develop end-stage renal disease due to progressive glomerulosclerosis. We previously showed that the subcutaneous injection of a non-anticoagulant heparin reduced glomerulosclerosis in bGH mice. Since injectable drugs are not a practical means of controlling glomerulosclerosis in humans, we assessed oral heparin-like compounds and found that oral pentosan polysulfate (PPS) reduced glomerulosclerosis in bGH mice at non-toxic doses. Because the positive therapeutic response in the bGH model could have been principally hormone-mediated, we examined other models of non-immune mediated glomerulosclerosis, including ROP Os/+ non-diabetic and diabetic mice. We found that an oral PPS (Elmiron), which is approved for other indications in humans, reduced sclerosis in all of these forms of chronic, progressive glomerulosclerosis. Based on the similarity of the cellular events in glomerulosclerosis and arteriosclerosis, we assessed the effect(s) of PPS in congenital (Watanabe rabbits) and induced (New Zealand White lipid-fed rabbits) models of arteriosclerosis. The extent and severity of the lesions was significantly reduced in both models by PPS treatment. Finally, we asked whether the proliferative and sclerotic lesion, which is the cause of vascular graft stenosis, might also respond to PPS treatment. To do this we cultured cells from the materials removed from stenotic arteriovenous grafts in hemodialysis patients. We found that PPS inhibits the proliferation and matrix production in a dose-dependent manner.
- Published
- 1997
50. Renal microangiopathy in diabetes.
- Author
-
Striker LJ
- Subjects
- Animals, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1 complications, Diabetic Angiopathies genetics, Diabetic Nephropathies genetics, Extracellular Matrix drug effects, Extracellular Matrix metabolism, Glycation End Products, Advanced pharmacology, Glycosuria, Renal genetics, In Vitro Techniques, Mice, Mice, Inbred NOD, Diabetic Angiopathies complications, Diabetic Nephropathies complications, Glycosuria, Renal complications
- Published
- 1997
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.