1. Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction.
- Author
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Brito LC, DalBó S, Striechen TM, Farias JM, Olchanheski LR Jr, Mendes RT, Vellosa JC, Fávero GM, Sordi R, Assreuy J, Santos FA, and Fernandes D
- Subjects
- Acetylcholine pharmacology, Alveolar Bone Loss physiopathology, Analysis of Variance, Animals, Biomarkers analysis, C-Reactive Protein analysis, Endothelium metabolism, Inflammation metabolism, Inflammation physiopathology, Interleukin-6 blood, Male, Mesenteric Arteries physiopathology, Microscopy, Fluorescence, Nitric Oxide Synthase analysis, Nitroprusside pharmacology, Periodontitis metabolism, Phenylephrine pharmacology, Rats, Rats, Wistar, Superoxides analysis, Endothelium drug effects, Inflammation complications, Mesenteric Arteries drug effects, Periodontitis etiology, Vasodilation drug effects
- Abstract
Objectives: This study aimed to evaluate the systemic inflammatory response and cardiovascular changes induced by experimental periodontitis in rats., Design: Experimental periodontitis was induced by placing a cotton ligature around the cervix of both sides of mandibular first molars and maxillary second molars in each male rat. Sham-operated rats had the ligature removed immediately after the procedure. Seven, 14 or 28 days after procedure, the effects of acetylcholine, sodium nitroprusside and phenylephrine were evaluated on blood pressure, aortic rings and isolated and perfused mesenteric bed. The blood was obtained for plasma Interleukin-6 (IL-6), C-reactive protein (CRP) and lipid evaluation. The mesenteric vessels were obtained to evaluate superoxide production and nitric oxide synthase 3 (NOS-3) expression., Results: Ligature induced periodontitis reduced endothelium-dependent vasodilatation, a hallmark of endothelial dysfunction. This effect was associated with an increase in systemic inflammatory markers (IL-6 and CRP), worsens on lipid profile, increased vascular superoxide production and reduced NOS-3 expression. It is interesting to note that many of these effects were transitory., Conclusion: Periodontitis induced a transient systemic and vascular inflammation which leads to endothelial dysfunction, an initial step for cardiovascular diseases. Moreover, the animal model of periodontitis used here may represent a valuable tool for studying the relationship between periodontitis and endothelial dysfunction., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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