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5 results on '"Stream COST Action FA1206"'

1. Strigolactones (SLs) modulate the plastochron by regulating KLUH (KLU) transcript abundance in Arabidopsis

Author

Cornet, Florent, Pillot, Jean‐Paul, Le Bris, Philippe, Pouvreau, Jean‐Bernard, Arnaud, Nicolas, Saint Germain, Alexandre, Rameau, Catherine, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universite Paris-Saclay, Orsay 91405, France, Laboratoire de biologie et pathologie végétales (LBPV), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN), Stream COST Action FA1206, ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), ANR-12-BSV6-0017,INFERNO,Hérédité épigénétique transgénérationnelle chez les ciliés : mécanismes et conséquences évolutives(2012), and ANR-12-BSV6-0004,StrigoPath,Voies de signalisation des strigolactones (et/ou de dérivés) chez les plantes terrestres(2012)

Subjects
Arabidopsis, food and beverages, CYP78A, shoot branching, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, strigolactones, plant hormone, plastochron
Abstract

International audience; The timing of leaf emergence at the shoot apical meristem, or plastochron, is highly regulated in plants. Among the genes known to regulate the plastochron in Arabidopsis (Arabidopsis thaliana), KLUH (KLU), orthologous to the rice (Oryza sativa) PLASTOCHRON1, encodes the cytochrome P450 CYP78A5, and is thought to act through generation of a still unknown mobile signal. As klu mutants display not only a short plastochron but also a branching phenotype reminiscent of strigolactone (SL) mutants, we investigated whether KLU/CYP78A5 is involved in SL biosynthesis. We combined a genetic approach, a parasitic plant seed germination bioassay to test klu root exudates, and analysis of transcript abundances of SL-biosynthesis genes in the Arabidopsis klu mutants. We demonstrate that KLU is not involved in the SL-biosynthesis pathway. Moreover, this work allowed us to uncover a new role for SL during Arabidopsis development in modulating plastochron via a KLU-dependent pathway. Globally our data reveal that KLU is required for plastochron-specific SL responses, a first indication of crosstalk between SL and the KLU-derived signal.

Published
2021

2. Validated Method for Strigolactone Quantification by Ultra High-Performance Liquid Chromatography - Electrospray Ionisation Tandem Mass Spectrometry Using Novel Deuterium Labelled Standards

Author

Boutet-Mercey, Stephanie, Perreau, François, Roux, Amélie, Clavé, Guillaume, Pillot, Jean-Paul, Schmitz-Afonso, Isabelle, Touboul, David, Mouille, Gregory, Rameau, Catherine, Boyer, Francois-Didier, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Littoral, Environnement, Télédétection, Géomatique (LETG-Angers), Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN), Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN), Université de Nantes (UN)-Université de Nantes (UN), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Agence Nationale de la Recherche [ANR-12-BSV6-004-01], la Region Ile de France, le department des Yvelines, Stream COST Action [FA1206], Labex Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], Littoral, Environnement, Télédétection, Géomatique - Laboratoire d’Etudes Environnementales des Systèmes Anthropisés (LETG-ANGERS), Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-Université de Nantes (UN)-École pratique des hautes études (EPHE)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Strigolactones, Spectrometry, Mass, Electrospray Ionization, integumentary system, pea, Peas, deuterium-labelled strigolactones, Reproducibility of Results, Deuterium, Plant Roots, Lactones, Tandem Mass Spectrometry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Isotope Labeling, plant hormones, [CHIM]Chemical Sciences, Chromatography, Liquid
Abstract

International audience; Introduction =Strigolactones (SLs) are important plant hormones. They are difficult to analyse because they occur in very small concentrations especially in comparison with other plant hormones and other substances can interfere with their detection.Objective =To develop a procedure for the extraction, purification and quantification of SLs from plant roots.Methodology =Samples were prepared by extraction of plant root tissues with ethyl acetate. Then the extracts were further purified with silica column chromatography. The natural SLs in the final extracts were quantified using novel deuterium labelled SLs. The results of the methodology were compared with those of the procedure of Yoneyama and coworkers.Results =This procedure required about 1‐g root samples to detect and quantify simultaneously the SLs (orobanchyl acetate and fabacyl acetate) concentration with high reliability.Conclusion =A method was developed for determining endogenous fabacyl acetate and orobanchyl acetate in plant tissue based on novel deuterium labelled standards. A method of orobanchol quantification using a synthetic SL GR24 as internal standard was proposed.

Published
2018

3. The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop

Author

Yasmine Ligerot, Alexandre de Saint Germain, Tanya Waldie, Christelle Troadec, Sylvie Citerne, Nikita Kadakia, Jean-Paul Pillot, Michael Prigge, Grégoire Aubert, Abdelhafid Bendahmane, Ottoline Leyser, Mark Estelle, Frédéric Debellé, Catherine Rameau, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Sud - Paris 11 (UP11), Sainsbury Laboratory Cambridge University (SLCU), University of Cambridge [UK] (CAM), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), University of California [San Diego] (UC San Diego), University of California (UC), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Labex Saclay Plant Sciences -SPS : ANR-10-LABX-0040-SPS, Agence Nationale de la Recherche : ANR-12-BSV6-004-01, European Cooperation in Science and Technology (Stream COST Action) : FA1206, Ministere de la Recherche, European Research Council (ERC-SEXYPARTH), Ministere de l'Education Nationale, de la Recherche et de la Technologie (MENRT), Gatsby Charitable Foundation : GAT3272C, Leyser, Ottoline [0000-0003-2161-3829], Apollo - University of Cambridge Repository, University of California, and Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
Internodes, Arabidopsis, Gene Expression, Plant Science, biosynthèse, Biochemistry, Plant Growth Regulators, Cell Signaling, Gene Expression Regulation, Plant, Membrane Receptor Signaling, Plant Hormones, Plant Proteins, Vegetal Biology, Plant Biochemistry, Plant Anatomy, Eukaryota, food and beverages, Plants, Legumes, Hormone Receptor Signaling, Experimental Organism Systems, Plant Shoots, Signal Transduction, Research Article, lcsh:QH426-470, Picloram, Receptors, Cell Surface, Brassica, Research and Analysis Methods, Biosynthesis, Model Organisms, Plant and Algal Models, Medicago truncatula, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Grasses, amélioration génétique, Indoleacetic Acids, auxine, Arabidopsis Proteins, arabidopsis thaliana, fungi, Peas, Organisms, Biology and Life Sciences, Cell Biology, Stem Anatomy, Hormones, lcsh:Genetics, pois, Auxins, Rice, Biologie végétale
Abstract

Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4) mutants indicative of negative feedback control. In contrast, the highly branched rms2 mutant has reduced transcript levels of SL biosynthesis genes. Grafting studies and hormone quantification led to a model where RMS2 mediates a shoot-to-root feedback signal that regulates both SL biosynthesis gene transcript levels and xylem sap levels of cytokinin exported from roots. Here we cloned RMS2 using synteny with Medicago truncatula and demonstrated that it encodes a putative auxin receptor of the AFB4/5 clade. Phenotypes similar to rms2 were found in Arabidopsis afb4/5 mutants, including increased shoot branching, low expression of SL biosynthesis genes and high auxin levels in stems. Moreover, afb4/5 and rms2 display a specific resistance to the herbicide picloram. Yeast-two-hybrid experiments supported the hypothesis that the RMS2 protein functions as an auxin receptor. SL root feeding using hydroponics repressed auxin levels in stems and down-regulated transcript levels of auxin biosynthesis genes within one hour. This auxin down-regulation was also observed in plants treated with the polar auxin transport inhibitor NPA. Together these data suggest a homeostatic feedback loop in which auxin up-regulates SL synthesis in an RMS2-dependent manner and SL down-regulates auxin synthesis in an RMS3 and RMS4-dependent manner., Author summary Plant shoot branching results from the precise regulation of bud growth versus dormancy. Positive and negative feedback mechanisms are likely involved in the dynamic control of this highly plastic trait. Strigolactones, the most recently discovered class of plant hormones, play a key role in controlling shoot branching. Negative feedback control of strigolactone biosynthesis has been observed in several species and was shown in pea to be mediated by a shoot-to-root signal that is RAMOSUS2 (RMS2)-dependent. The chemical nature of this feedback signal has been extensively discussed. Here, we demonstrate that the RMS2 protein belongs to the small family of auxin receptors and confirm that it behaves as an auxin receptor. Strigolactones decrease stem auxin levels by rapidly repressing transcript levels of auxin biosynthesis genes, thereby forming a long-distance feedback loop between auxin and strigolactones for the precise regulation of shoot branching in plants.

Published
2017

4. An histidine covalent receptor and butenolide complex mediates strigolactone perception

Author

Frank Pelissier, Marie-Ange Badet-Denisot, Alexandre de Saint Germain, Marco Bürger, Colin G. N. Turnbull, Pascal Retailleau, Sandrine Bonhomme, David Cornu, Jean-Paul Pillot, Jean-Pierre Le Caer, Guillaume Clavé, Catherine Rameau, François-Didier Boyer, Joanne Chory, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), SICaPS (SICaPS), Département Plateforme (PF I2BC), Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, The Salk Institute for Biological Studies, Division of cell and molecular biology, Imperial College London, Unité de Recherche en Génétique et Amélioration des Plantes (UR254), Institut National de la Recherche Agronomique (INRA), Plant Biology Laboratory and Howard Hughes Medical Institute, Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche [ANR-12-BSV6-004-01], Stream COST Action [FA1206], US National Institutes of Health [R01 GM094428], Howard Hughes Medical Institute, Catharina Foundation, Labex Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], Terry L. Sheppard, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), and Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Biochemistry & Molecular Biology, Stereochemistry, Medicinal And Biomolecular Chemistry, Strigolactone, Biology, Ligands, 01 natural sciences, Article, Lactones, 03 medical and health sciences, 4-Butyrolactone, Plant Growth Regulators, Catalytic triad, [CHIM]Chemical Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Histidine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biochemistry And Cell Biology, Molecular Biology, Plant Proteins, Butenolide, Molecular Structure, Peas, food and beverages, Cell Biology, biology.organism_classification, Ligand (biochemistry), Karrikin, 030104 developmental biology, Biochemistry, Covalent bond, Plant hormone, Heterocyclic Compounds, 3-Ring, 010606 plant biology & botany
Abstract

International audience; Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of α/β-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.

Published
2016

5. Strigolactone biosynthesis and signaling in plant development

Author

Yasmine Ligerot, Mauricio Lopez-Obando, Catherine Rameau, Sandrine Bonhomme, François-Didier Boyer, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Agence Nationale de la Recherche [ANR-12-BSV6-004-01], and Stream COST Action FA1206

Subjects
0106 biological sciences, Ubiquitin proteasome system, [SDV]Life Sciences [q-bio], Strigolactone, Plant Development, Biology, 01 natural sciences, 03 medical and health sciences, Lactones, Hormone signaling, Shoot branching, Molecular Biology, 030304 developmental biology, Karrikins, 0303 health sciences, Rhizosphere, food and beverages, Strigolactone biosynthesis, Cell biology, Biosynthetic Pathways, Plant development, Biochemistry, Proteolysis, Signal transduction, Plant Shoots, 010606 plant biology & botany, Developmental Biology, Signal Transduction
Abstract

Strigolactones (SLs), first identified for their role in parasitic and symbiotic interactions in the rhizosphere, constitute the most recently discovered group of plant hormones. They are best known for their role in shoot branching but, more recently, roles for SLs in other aspects of plant development have emerged. In the last five years, insights into the SL biosynthetic pathway have also been revealed and several key components of the SL signaling pathway have been identified. Here, and in the accompanying poster, we summarize our current understanding of the SL pathway and discuss how this pathway regulates plant development.

Published
2015

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