21 results on '"Stranska-Zachariasova M"'
Search Results
2. Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market
- Author
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Jonatova, P., primary, Dzuman, Z., additional, Prusova, N., additional, Hajslova, J., additional, and Stranska-Zachariasova, M., additional
- Published
- 2020
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3. Developments in mycotoxin analysis: an update for 2017-2018
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Tittlemier, S.A., primary, Cramer, B., additional, Dall’Asta, C., additional, Iha, M.H., additional, Lattanzio, V.M.T., additional, Malone, R.J., additional, Maragos, C., additional, Solfrizzo, M., additional, Stranska-Zachariasova, M., additional, and Stroka, J., additional
- Published
- 2019
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4. Developments in mycotoxin analysis: an update for 2016-2017
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Berthiller, F., primary, Cramer, B., additional, Iha, M.H., additional, Krska, R., additional, Lattanzio, V.M.T., additional, MacDonald, S., additional, Malone, R.J., additional, Maragos, C., additional, Solfrizzo, M., additional, Stranska-Zachariasova, M., additional, Stroka, J., additional, and Tittlemier, S.A., additional
- Published
- 2018
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5. Developments in mycotoxin analysis: an update for 2015-2016
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Berthiller, F., primary, Brera, C., additional, Iha, M.H., additional, Krska, R., additional, Lattanzio, V.M.T., additional, MacDonald, S., additional, Malone, R.J., additional, Maragos, C., additional, Solfrizzo, M., additional, Stranska-Zachariasova, M., additional, Stroka, J., additional, and Tittlemier, S.A., additional
- Published
- 2017
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6. Development of a new LC-MS method for accurate and sensitive determination of 33 pyrrolizidine and 21 tropane alkaloids in plant-based food matrices.
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Dzuman Z, Jonatova P, Stranska-Zachariasova M, Prusova N, Brabenec O, Novakova A, Fenclova M, and Hajslova J
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- Chromatography, High Pressure Liquid methods, Isomerism, Limit of Detection, Reproducibility of Results, Tandem Mass Spectrometry methods, Alkaloids analysis, Food Contamination analysis, Plants chemistry, Pyrrolizidine Alkaloids analysis, Tropanes analysis
- Abstract
Setting of maximum limits for a number of plant alkaloids is under discussion in the EU. The novel method developed and optimized in this study enables simultaneous determination of 21 tropane alkaloids (TAs) and 33 pyrrolizidine (PAs) together with their N-oxides (PANOs). For analysis of aqueous-methanolic extract, reversed phase ultra-high-performance liquid chromatography and tandem mass spectrometry (RP-U-HPLC-MS/MS) was employed. The method was validated for frequently contaminated matrices (i) sorghum, (ii) oregano, and (iii) mixed herbal tea. The recoveries at two spiking levels were in the range of 82-115%, 80-106%, and 78-117%, respectively, and repeatabilities were less than 19% for all analyte/matrix combinations. As regards the achieved limits of quantification (LOQ), their values were in the range of 0.5-10 μg kg
-1 . The crucial problem encountered during method development, co-elution of multiple groups of isomeric alkaloids, was overcome by subsequent sample separation in the second chromatographic system, hydrophilic interaction liquid chromatography (HILIC), providing different separation selectivity. Lycopsamine, echinatine, and indicine (co-elution group 1) and N-oxides of indicine and intermedine (co-elution group 2), which could not be resolved on the commonly used RP column, were possible to separate fully by using the HILIC system.- Published
- 2020
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7. In Silico and In Vitro Studies of Mycotoxins and Their Cocktails; Their Toxicity and Its Mitigation by Silibinin Pre-Treatment.
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Tran VN, Viktorova J, Augustynkova K, Jelenova N, Dobiasova S, Rehorova K, Fenclova M, Stranska-Zachariasova M, Vitek L, Hajslova J, and Ruml T
- Subjects
- ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Animals, Cell Line, Cell Survival drug effects, Coculture Techniques, Comet Assay, Computer Simulation, Dietary Supplements, Drug Interactions, Humans, Mice, Silybum marianum chemistry, Mycotoxins toxicity, Protective Agents pharmacology, Silybin pharmacology
- Abstract
Mycotoxins found in randomly selected commercial milk thistle dietary supplement were evaluated for their toxicity in silico and in vitro. Using in silico methods, the basic physicochemical, pharmacological, and toxicological properties of the mycotoxins were predicted using ACD/Percepta. The in vitro cytotoxicity of individual mycotoxins was determined in mouse macrophage (RAW 264.7), human hepatoblastoma (HepG2), and human embryonic kidney (HEK 293T) cells. In addition, we studied the bioavailability potential of mycotoxins and silibinin utilizing an in vitro transwell system with differentiated human colon adenocarcinoma cells (Caco-2) simulating mycotoxin transfer through the intestinal epithelial barrier. The IC
50 values for individual mycotoxins in studied cells were in the biologically relevant ranges as follows: 3.57-13.37 nM (T-2 toxin), 5.07-47.44 nM (HT-2 toxin), 3.66-17.74 nM (diacetoxyscirpenol). Furthermore, no acute toxicity was obtained for deoxynivalenol, beauvericin, zearalenone, enniatinENN-A, enniatin-A1, enniatin-B, enniatin-B1, alternariol, alternariol-9-methyl ether, tentoxin, and mycophenolic acid up to the 50 nM concentration. The acute toxicity of these mycotoxins in binary combinations exhibited antagonistic effects in the combinations of T-2 with DON, ENN-A1, or ENN-B, while the rest showed synergistic or additive effects. Silibinin had a significant protective effect against both the cytotoxicity of three mycotoxins (T-2 toxin, HT-2 toxin, DAS) and genotoxicity of AME, AOH, DON, and ENNs on HEK 293T. The bioavailability results confirmed that AME, DAS, ENN-B, TEN, T-2, and silibinin are transported through the epithelial cell layer and further metabolized. The bioavailability of silibinin is very similar to mycotoxins poor penetration.- Published
- 2020
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8. Liquid chromatography-drift tube ion mobility-mass spectrometry as a new challenging tool for the separation and characterization of silymarin flavonolignans.
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Fenclova M, Stranska-Zachariasova M, Benes F, Novakova A, Jonatova P, Kren V, Vitek L, and Hajslova J
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- Flavonolignans analysis, Flavonolignans isolation & purification, Isomerism, Mass Spectrometry methods, Silymarin isolation & purification, Chromatography, High Pressure Liquid methods, Ion Mobility Spectrometry methods, Silybum marianum chemistry, Silymarin analysis
- Abstract
Silymarin, milk thistle (Silybum marianum) extract, contains a mixture of mostly isomeric bioactive flavonoids and flavonolignans that are extensively studied, especially for their possible liver-protective and anticancer effects. Because of the differing bioactivities of individual isomeric compounds, characterization of their proportion in a mixture is highly important for predicting its effect on health. However, because of silymarin's complexity, this is hardly feasible by common analytical techniques. In this work, ultraperformance liquid chromatography coupled with drift tube ion mobility spectrometry and quadrupole time-of-flight mass spectrometry was used. Eleven target silymarin compounds (taxifolin, isosilychristin, silychristins A and B, silydianin, silybins A and B, 2,3-cis-silybin B, isosilybins A and B and 2,3-dehydrosilybin) and five unknown flavonolignan isomers detected in the milk thistle extract were fully separated in a 14.5-min analysis run. All the compounds were characterized on the basis of their accurate mass, retention time, drift time, collision cross section and fragmentation spectra. The quantitative approach based on evaluation of the ion mobility data demonstrated lower detection limits, an extended linear range and total separation of interferences from the compounds of interest compared with the traditional approach based on evaluation of liquid chromatography-quadrupole time-of-flight mass spectrometry data. The following analysis of a batch of milk thistle-based food supplements revealed significant variability in the silymarin pattern, especially in the content of silychristin A and silybins A and B. This newly developed method might have high application potential, especially for the characterization of materials intended for bioactivity studies in which information on the exact silymarin composition plays a crucial role. Graphical Abstract.
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- 2020
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9. Corrigendum to "Cranberries versus lingonberries: A challenging authentication of similar Vaccinium fruit" [Food Chem. 284 (2019) 162-170].
- Author
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Hurkova K, Uttl L, Rubert J, Navratilova K, Kocourek V, Stranska-Zachariasova M, Paprstein F, and Hajslova J
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- 2019
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10. Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements.
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Viktorova J, Stranska-Zachariasova M, Fenclova M, Vitek L, Hajslova J, Kren V, and Ruml T
- Abstract
Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many of them generate contradictory and non-compliant results. In our study, we focused on the characterization of traditionally used biochemical (2,2'-azino- bis- (3-ethylbenzothiazoline-6 sulfonic acid) (ABTS), Oxygen Radical Absorption Capacity (ORAC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and cellular (CAA) antioxidant tests on a broad set of milk thistle dietary supplements containing silymarin. In addition to 26 commercially available preparations, also the natural silymarin extract available from Sigma Aldrich, St. Louis, MI, USA, and a model mixture of pure flavonoid/flavonolignans mimicking the silymarin composition were investigated as control samples. Significant differences in the antioxidant capacity of the supplements were observed. Unlike the DPPH, the results of the ABTS and ORAC methods correlated with the silymarin components determined by U-HPLC-HRMS/MS. The responses in CAA were considerably lower than in other assays. Silymarin exhibited a significantly higher antioxidant capacity than the artificially prepared flavonoid/flavonolignans mixture in all tests, indicating possible presence of other antioxidants of natural origin. The follow-up U-HPLC-HRMS/MS screening revealed the presence of tens of non-silymarin compounds with reported antioxidant activity (not only in the silymarin extract, but also in the milk thistle preparations). The sum of the total phenolics and the sum of the simple phenolics correlated with CAA results more than silymarin.
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- 2019
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11. Poor chemical and microbiological quality of the commercial milk thistle-based dietary supplements may account for their reported unsatisfactory and non-reproducible clinical outcomes.
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Fenclova M, Novakova A, Viktorova J, Jonatova P, Dzuman Z, Ruml T, Kren V, Hajslova J, Vitek L, and Stranska-Zachariasova M
- Subjects
- Antioxidants chemistry, Antioxidants physiology, Biological Products pharmacology, Humans, Liver Diseases drug therapy, Mycotoxins chemistry, Pesticides chemistry, Phytotherapy methods, Plant Extracts pharmacology, Silymarin chemistry, Silymarin pharmacology, Biological Products chemistry, Dietary Supplements microbiology, Silybum marianum chemistry, Silybum marianum microbiology, Plant Extracts chemistry
- Abstract
Herbal-based dietary supplements have become increasingly popular. The extract from milk thistle (Silybum marianum), is often used for the treatment of liver diseases. However, serious concerns exist regarding the efficacy, composition, as well as the safety of these over-the-counter preparations. Therefore, the aim of the present study was to investigate the composition as well as chemical and biological safety of 26 milk thistle-based dietary supplements purchased from both the U.S. and Czech markets between 2016 and 2017. The study was focused on a determination of the composition of active ingredients, as well as analyses of possible contaminants including: mycotoxins, plant alkaloids, and pesticide residues, as well as the microbial purity. High-throughput analyses were performed using advanced U-HPLC-HRMS techniques. Large differences in the silymarin content were observed among individual milk thistle preparations, often in contrast with the information provided by the manufacturers. In addition, substantial inter-batch differences in silymarin content were also demonstrated. In all milk thistle preparations tested, large numbers and high concentrations of mycotoxins and several pesticides, as well as the substantial presence of microbiological contamination were detected, pointing to serious safety issues. In conclusion, our results strongly indicate the need for strict controls of the composition, chemical contaminants, as well as the microbiological purity of commercial milk thistle extracts used for the treatment of liver diseases. Poor definition of these preparations together with contamination by biologically active substances may not only account for the inconsistency of clinical observations, but also be responsible for possible herbal-based dietary supplements-induced liver injury.
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- 2019
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12. Cranberries versus lingonberries: A challenging authentication of similar Vaccinium fruit.
- Author
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Hurkova K, Uttl L, Rubert J, Navratilova K, Kocourek V, Stranska-Zachariasova M, Paprstein F, and Hajslova J
- Subjects
- Biomarkers analysis, Chromatography, High Pressure Liquid, Flavonoids analysis, Food Analysis statistics & numerical data, Food Contamination, Glucosides analysis, Models, Statistical, Phospholipids analysis, Phytochemicals analysis, Plant Extracts analysis, Polyphenols analysis, Tandem Mass Spectrometry, Vaccinium macrocarpon metabolism, Vaccinium vitis-idaea metabolism, Food Analysis methods, Metabolomics methods, Vaccinium macrocarpon chemistry, Vaccinium vitis-idaea chemistry
- Abstract
Due to unique phytochemicals contained, Vaccinum berries are known to have a number of positive health effects. In this context, lingonberries (Vaccinium vitis-idaea) are considered to be the most effective, thus finding many uses. Recently, fraud suspicion on lingonberries-based products has been reported, partial or even total replacement by less valued cranberries (Vaccinium macrocarpon) was found. In this study, metabolomic fingerprinting employing instrumental platform consisting of U-HPLC-HRMS/MS was investigated for discrimination between the two Vaccinum berries species. Methanolic extracts of 33 authentic samples from two harvest years were analyzed and chemometric evaluation was performed to identify significant marker compounds, their stability during drying process was assessed, too. The characteristic markers most contributing to berries classification were representatives of polyphenols and phospholipids. Peonidin 3-O-arabinoside and myricetin 3-O-glucoside, not occurring in lingonberries, enabled to discover the presence of cranberries in prepared admixtures down to 1% (w/w)., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
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- 2019
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13. Analysis of phosphodiesterase type 5 inhibitors as possible adulterants of botanical-based dietary supplements: extensive survey of preparations available at the Czech market.
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Jiru M, Stranska-Zachariasova M, Dzuman Z, Hurkova K, Tomaniova M, Stepan R, Cuhra P, and Hajslova J
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- Chromatography, High Pressure Liquid methods, Czech Republic, Dietary Supplements analysis, Drug Contamination prevention & control, Limit of Detection, Phytochemicals analysis, Tandem Mass Spectrometry methods, Counterfeit Drugs analysis, Dietary Supplements standards, Fraud prevention & control, Phosphodiesterase 5 Inhibitors analysis, Phytochemicals standards
- Abstract
Popularity of natural-based preparations supporting the sexual potency significantly increased in recent years, which also led to the increase of illegal use of phosphodiesterase type 5 inhibitor (PDE-5) in sexual performance enhancement products. In this study, a rapid U-HPLC‒HRMS/MS method has been developed to simultaneously determine 59 PDE-5 inhibitors and their analogues. Within the development of sensitive method for analysis of 59 PDE-5 inhibitors and their analogues, both sample preparation procedure, as well as separation / detection conditions have been optimized. Extraction efficiency of particular extraction solvents, influence of different mobile phase additives on target analytes separation, as well as impact of various settings of mass analyzer on sensitivity of detection were examined. Data were collected in the 'full MS/data dependent MS/MS' acquisition mode (full MS-dd-MS/MS). Before the U-HPLC‒HRMS/MS method was used for analysis of real samples, proper validation had been conducted. The precision of the method expressed as the relative standard deviation (RSD) was ≤4.2% and ≤5.2% at spiking concentrations 5 μg/g and 0.25 μg/g, respectively. The limits of quantification were in the range 0.25 - 0.05 μg/g and the recovery ranged between 71 and 90%. The optimized method was successfully applied for analysis of 64 real samples, and 10 of them were proved to contain both registered or unregistered synthetic PDE-5 inhibitors. Additionally, the acquired U-HPLC‒HRMS/MS fingerprints were demonstrated to serve as an efficient tool for revealing of other type of possible fraud in products labeling. Retrospective mining of markers of herbs declared on dietary supplements packaging allowed to assess the trueness / untruth in the declaration of medical herbs composition., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2019
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14. High resolution-ion mobility mass spectrometry as an additional powerful tool for structural characterization of mycotoxin metabolites.
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Righetti L, Fenclova M, Dellafiora L, Hajslova J, Stranska-Zachariasova M, and Dall'Asta C
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- Glucuronides chemistry, Glucuronides metabolism, Humans, Molecular Structure, Mass Spectrometry methods, Mycotoxins chemistry, Mycotoxins metabolism
- Abstract
This work was designed as a proof of concept, to demonstrate the successful use of the comparison between theoretical and experimental collision cross section (CCS) values to support the identification of isomeric forms. To this purpose, thirteen mycotoxins were considered and analyzed using drift time ion mobility mass spectrometry. A good linear correlation (r
2 = 0.962) between theoretical and experimental CCS was found. The average ΔCCS was 3.2%, fully consistent with the acceptability threshold value commonly set at 5%. The agreement between theoretical and experimental CCS obtained for mycotoxin glucuronides suggested the potential of the CCS matching in supporting the annotation procedure., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
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15. A novel approach based on untargeted lipidomics reveals differences in the lipid pattern among durum and common wheat.
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Righetti L, Rubert J, Galaverna G, Hurkova K, Dall'Asta C, Hajslova J, and Stranska-Zachariasova M
- Subjects
- Lipids, Metabolomics, Triticum
- Abstract
In the present work the possibility of using an untargeted metabolomic strategy to discriminate between common and durum wheat lipidome for an authenticity purpose was explored. A first study was conducted by analyzing 52 samples from two durum and common wheat varieties. Afterwards, an extended and independent sample set (173 samples and five varieties) was used as a confirmatory study to verify the stability and consistency of the models obtained. Putatively identified markers were evaluated applying ROC curves resulting in individual marker AUC >90% both in preliminary and confirmatory study. In addition, digalactosyl diglyceride (DGDG) 36:4 was shown to be an effective marker differentiating between authentic durum wheat and its adulterated admixture down to 3% adulteration level, which is the maximum contamination level allowed by Italian legislation. The results demonstrated that untargeted lipidomics, in conjunction with chemometric tools has a significant potential for screening and detection of wheat fraud., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2018
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16. Untargeted metabolomics based on ultra-high-performance liquid chromatography-high-resolution mass spectrometry merged with chemometrics: A new predictable tool for an early detection of mycotoxins.
- Author
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Rubert J, Righetti L, Stranska-Zachariasova M, Dzuman Z, Chrpova J, Dall'Asta C, and Hajslova J
- Subjects
- DNA, Fungal isolation & purification, Food Microbiology, Fusarium isolation & purification, Principal Component Analysis, Triticum chemistry, Chromatography, High Pressure Liquid, Food Contamination analysis, Mass Spectrometry, Metabolomics methods, Mycotoxins analysis, Triticum microbiology
- Abstract
In order to explore the early detection of mycotoxins in wheat three standardized approaches (Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxin quantification) and a novel untargeted metabolomics strategy were jointly assessed. In the first phase of this research, standardized approaches were able to quantify mycotoxins and identify Fusarium spp. Then, an UHPLC-QTOF metabolic fingerprinting method was developed to investigate plant-pathogen cross-talk. At the same time, chemometrics analysis demonstrated to be a powerful tool in order to distinguish low and strong infection levels. Combining these results, the cross-talk plant pathogen related to the early detection of mycotoxins was discovered. As a rapid response to fungal infection an overexpression of phosphatidic acids was discovered. By contrast, when the infection became stronger an increase of oxylipins and diacylglycerols was revealed., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2017
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17. Corrigendum to "Bioprospecting of microalgae: Proper extraction followed by high performance liquid chromatographic-high resolution mass spectrometric fingerprinting as key tools for successful metabolom characterization" [J. Chromatogr. B 1015-1016 (2016) 22-23].
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Stranska-Zachariasova M, Kastanek P, Dzuman Z, Rubert J, Godula M, and Hajslova J
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- 2017
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18. Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds.
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Stranska-Zachariasova M, Kurniatanty I, Gbelcova H, Jiru M, Rubert J, Nindhia TG, D'Acunto CW, Sumarsono SH, Tan MI, Hajslova J, and Ruml T
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- Cells, Cultured, Chromatography, High Pressure Liquid, Humans, Lipids analysis, Lipids chemistry, Metabolome, Metabolomics, Microalgae metabolism, Tandem Mass Spectrometry, Antioxidants pharmacology, Biological Products chemistry, Bioprospecting methods, Cell Proliferation drug effects, Microalgae chemistry, Protective Agents pharmacology
- Abstract
The present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e., H
2 O, MeOH/H2 O (80:20, v/v) and hexane/i-PrOH (50:50, v/v), were used for extraction. Both the biological activity and the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix/breast/pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed. To complete the information about macroalgae composition, organic compounds were characterized by the liquid chromatography coupled with high resolution tandem mass spectrometry. Attention was concentrated mainly on the lipidomic profile characterization. In spite the fact that any significant antiproliferative effect was not observed for cancer cells, both the Turbinaria species were shown to be good protectors against the oxidative stress of the non-cancer cells. Most of the antioxidants were determined in the hexane/i-PrOH extract. As regards the lipids identified, most of them belonged to the triacylglycerols followed by sphingomyelins, diacylglycerols, and polar (lyso)phospholipids. Additionally to fatty acids with 14, 16 and 18 carbons, also those with odd carbon numbers were frequently present., (© 2017 The Authors Chemistry & Biodiversity Published by Wiley-VHCA AG.)- Published
- 2017
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19. Characterization and Discrimination of Ancient Grains: A Metabolomics Approach.
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Righetti L, Rubert J, Galaverna G, Folloni S, Ranieri R, Stranska-Zachariasova M, Hajslova J, and Dall'Asta C
- Subjects
- Chromatography, High Pressure Liquid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Metabolomics methods, Triticum classification, Triticum metabolism
- Abstract
Hulled, or ancient, wheats were the earliest domesticated wheats by mankind and the ancestors of current wheats. Their cultivation drastically decreased during the 1960s; however, the increasing demand for a healthy and equilibrated diet led to rediscovering these grains. Our aim was to use a non-targeted metabolomic approach to discriminate and characterize similarities and differences between ancient Triticum varieties. For this purpose, 77 hulled wheat samples from three different varieties were collected: Garfagnana T. turgidum var. dicoccum L. (emmer), ID331 T. monococcum L. (einkorn) and Rouquin T. spelta L. (spelt). The ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry (UHPLC-QTOF) metabolomics approach highlighted a pronounced sample clustering according to the wheat variety, with an excellent predictability (Q²), for all the models built. Fifteen metabolites were tentatively identified based on accurate masses, isotopic pattern, and product ion spectra. Among these, alkylresorcinols (ARs) were found to be significantly higher in spelt and emmer, showing different homologue composition. Furthermore, phosphatidylcholines (PC) and lysophosphatidylcholines (lysoPC) levels were higher in einkorn variety. The results obtained in this study confirmed the importance of ARs as markers to distinguish between Triticum species and revealed their values as cultivar markers, being not affected by the environmental influences.
- Published
- 2016
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20. Fate of Free and Conjugated Mycotoxins within the Production of Distiller's Dried Grains with Solubles (DDGS).
- Author
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Dzuman Z, Stranska-Zachariasova M, Vaclavikova M, Tomaniova M, Veprikova Z, Slavikova P, and Hajslova J
- Subjects
- Chromatography, Liquid, Fermentation, Mycotoxins isolation & purification, Tandem Mass Spectrometry, Yeasts metabolism, Zea mays microbiology, Animal Feed analysis, Food Contamination analysis, Mycotoxins chemistry, Zea mays chemistry
- Abstract
Contamination of feed with mycotoxins represents a serious worldwide problem concerning animal health and related economic losses. The present paper provides comprehensive knowledge about the fate of mycotoxins during the production of distiller's dried grains with solubles (DDGS). The study was carried out using naturally infected maize material in five repetitions. For mycotoxin analysis, a QuEChERS-like ("Quick, Easy, Cheap, Effective, Rugged, and Safe") isolation approach and ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used. A significant increase of deoxynivalenol (DON) and its glycosylated form, DON-3-glucoside (DON-3-Glc), was observed during the first part of fermentation, when hydrolytic enzymes were added. After yeast addition, the total DON content rapidly decreased. An opposite trend was observed for fumonisin B1 (FB1), in which yeast addition contributed to increase of its content. Further considerable change in mycotoxin content occurred during the drying step, in which approximately two-thirds of the original content was lost.
- Published
- 2016
- Full Text
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21. Bioprospecting of microalgae: Proper extraction followed by high performance liquid chromatographic-high resolution mass spectrometric fingerprinting as key tools for successful metabolom characterization.
- Author
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Stranska-Zachariasova M, Kastanek P, Dzuman Z, Rubert J, Godula M, and Hajslova J
- Subjects
- Lipids chemistry, Metabolome, Metabolomics methods, Chromatography, High Pressure Liquid methods, Lipids analysis, Microalgae chemistry, Microalgae metabolism, Tandem Mass Spectrometry methods
- Abstract
Currently, the interest in microalgae as a source of biologically active components exploitable as supplementary ingredients to food/feed or in cosmetics continues to increase. Existing research mainly aims to focus on revealing and recovering the rare, cost competitive components of the algae metabolom. Because these components could be of very different physicochemical character, a universal approach for their isolation and characterization should be developed. This study demonstrates the systematic development of the extraction strategy that represents one of the key challenges in effective algae bioprospecting, which predefines their further industrial application. By using of Trachydiscus minutus as a model microalgae biomass, following procedures were tested and critically evaluated in order to develop the generic procedure for microalgae bioprospecting: (i) various ways of mechanical disintegration of algae cells enabling maximum extraction efficiency, (ii) the use of a wide range of extraction solvents/solvent mixtures suitable for optimal extraction yields of polar, medium-polar, and non-polar compounds, (iii) the use of consecutive extractions as a fractionation approach. Within the study, targeted screening of selected compounds representing broad range of polarities was realized by ultra-high performance liquid chromatography coupled with high resolution tandem mass spectrometric detection (UHPLC-HRMS/MS), to assess the effectiveness of undertaken isolation steps. As a result, simple and high-throughput extraction-fractionation strategy based on consecutive extraction with water-aqueous methanol-hexane/isopropanol was developed. Moreover, to demonstrate the potential of the UHPLC-HRMS/MS for the retrospective non-target screening and compounds identification, the collected mass spectra have been evaluated to characterize the pattern of extracted metabolites. Attention was focused on medium-/non-polar extracts and characterization of lipid species present in the T. minutus algae. Such detailed information on the composition of native (non-hydrolyzed) lipids of this microalga has not been published yet., (Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
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