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25 results on '"Stirpe, Mariarita"'

11. New perspectives from South-Y-East, not all about death. A report of the 12lnternational Meeting on Yeast Apoptosis in Bari, Italy, May 14th-18th, 2017

Author

Guaragnella, Nicoletta, Stirpe, Mariarita, Burhans, William, Côrte-Real, Manuela, Gourlay, Campbell W., Ludovico, Paula, Madeo, Frank, Petranovic, Dina, Winderickx, Joris, Mazzoni, Cristina, Giannattasio, Sergio, Guaragnella, Nicoletta, Stirpe, Mariarita, Burhans, William, Côrte-Real, Manuela, Gourlay, Campbell W., Ludovico, Paula, Madeo, Frank, Petranovic, Dina, Winderickx, Joris, Mazzoni, Cristina, and Giannattasio, Sergio

Published
2018

12. New perspectives from South-Y-East, not all about death A report of the 12th lnternational Meeting on Yeast Apoptosis in Bari, Italy, May 14th-18th, 2017

Author

Guaragnella, Nicoletta, primary, Stirpe, Mariarita, additional, Burhans, William, additional, Corte-Real, Manuela, additional, Gourlay, Campbell, additional, Ludovico, Paula, additional, Madeo, Frank, additional, Petranovic, Dina, additional, Winderickx, Joris, additional, Mazzoni, Cristina, additional, and Giannattasio, Sergio, additional

Published
2018
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24. Annurca apple (M. pumila Miller cv Annurca) extracts act against stress and ageing in S. cerevisiae yeast cells

Author

Ettore Novellino, Mariarita Stirpe, Romano Silvestri, Vanessa Palermo, Michele M. Bianchi, Cristina Mazzoni, Gian Carlo Tenore, Claudio Falcone, Stirpe, Mariarita, Palermo, Vanessa, Bianchi, Michele Maria, Silvestri, Romano, Falcone, Claudio, Tenore, GIAN CARLO, Novellino, Ettore, and Mazzoni, Cristina

Subjects
Polyphenol, 0301 basic medicine, Aging, Malus, Antioxidant, medicine.medical_treatment, anti-oxidant, apoptosis, fruit, mitochondria, polyphenols, ROS, Saccharomyces cerevisiae, Mitochondrion, Biology, medicine.disease_cause, Models, Biological, Antioxidants, Plant Extract, 03 medical and health sciences, medicine, Humans, Fragmentation (cell biology), chemistry.chemical_classification, Reactive oxygen species, 030102 biochemistry & molecular biology, Plant Extracts, Apoptosi, Oxidative Stre, lcsh:Other systems of medicine, General Medicine, lcsh:RZ201-999, biology.organism_classification, Yeast, Malu, Oxidative Stress, 030104 developmental biology, chemistry, Biochemistry, Complementary and alternative medicine, Reactive Oxygen Species, Reactive Oxygen Specie, Oxidative stress, Research Article, Human
Abstract

Background During the past years, a number of studies have demonstrated the positive effect of apple on ageing and different diseases such as cancer, degenerative and cardiovascular diseases. The unicellular yeast Saccharomyces cerevisiae represents a simple eukaryotic model to study the effects of different compounds on lifespan. We previously demonstrated that apple extracts have anti-ageing effects in this organism because of their antioxidant properties. In particular, the effect is related to the presence in this fruit of polyphenols, which give a large contribution to the antioxidant activity of apples. Methods We we used a clonogenic assay to assess the viability and the resistance to oxidative stress of S. cerevisiae cells in the presence of Annurca apple extracts. The production of ROS and the aberrant morphology of nuclei were detected by cell staining with the fluorescent dies Dihydrorhodamine 123 and DAPI, respectively. Mitochondrial morphology was analyzed by following the localization of the mito-GFP protein into the mitochondrial matrix. Results In this study, we show that apple extracts can increase yeast lifespan, reduce the levels of reactive oxygen species and cell sensitivity to oxidative stress, and prevent nuclei and mitochondria fragmentation protecting cells from regulated cell death. Conclusions In this paper, by using the yeast S. cerevisiae as a model, we have demonstrated that Annurca extracts possess antioxidant properties thanks to which the extracts can reduce the intracellular ROS levels and have anti-apoptotic functions thus prolonging cell lifespan. These results contribute to knowledge on the effects of natural compounds on ageing and support the use of yeast as a model organism for the development of simple tests to assess the effectiveness of bioactive substances from natural sources.

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