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PING Junior 2021 is organized with the support of funds for specific university research project SVK1-2021-008 and ERDF "Research of additive technologies for future applications in the machinery industry - RTI plus" (No. CZ.02.1.01/0.0/0.0/18_069/0010040). Laser ablation in liquids (LAL) has been developed into a simple and versatile technique producing various colloidal nanoparticles with novel properties and applications (e.g. biodetection, bioactive materials and photocatalysis). One of the typical problems faced in the bio/photo catalytic applications nanoparticles is their instability towards aggregation and the formation of larger particles, which change the catalytic properties and can even lead to inactive materials. A strong effort is therefore devoted to achieving a higher colloidal stability by various stabilizers or by suitable liquid media. However, the stabilizers are usually not environmentally friendly and freely moveable nanocatalysts can enter biogeochemical cycles and become harmful to the environment living organisms. It is therefore a novel and attractive idea to make use of the adsorption of nanoparticles on rough surfaces to bind these nanoparticles before they agglomerate in the liquid phase. In this simple way, colloidal photo/bio catalytic nanoparticles could adhere on a porous carrier, functionalize its surface. This unexplored potential of LAL we have revealed for laser ablation of FeS in water and ethanol which produces FeS-derived colloidal nanoparticles that absorb onto immersed porous ceramic substrates and create solar-light photocatalytic surfaces. The laser induced process thus offers an easy and efficient way for the functionalization of porous surfaces by photocatalytic nanoparticles that avoids aggregation in the liquid phase. Moreover, LAL induced functionalization has been found as perspective technique also for enhancing of bioactivity of titanium porous/textured surfaces. The importance of the synergy between the two disciplines: (i) chemical reactions of bone growth stimulants under LAL conditions and (ii) the functionalization of laser-shaped micro/nanotopography of titanium surface with colloidal particles generated from targets of bone growth stimulants by laser ablation in selected liquids is examined. The LAL synthesis of bioconductive colloidal particles of CaTiO3, MgTiO3 and CaSi2 allowing productivity, zeta potential, size and dispersity control through selected irradiation parameters and different liquid medium. The structure and adhesion of the nanosols to titanium surface is assessed by IR, Raman and XP spectroscopy and SEM and TEM analyses. In vitro osseointegration activity of the functionalized surfaces are evaluated by examining the growth of Messenchymal Stem cells.

PING 2019 is organized with the support of funds for specific university research project SVK1-2019-002. Despite the great progress that has been achieved in orthopedics biomaterials, fixation of implants to the bone host remains a problem. Mismatch of Young’s moduli of the biomaterials and the surrounding bone has been identified as a major reason for implant loosening following stress shielding of bone. However, the implanted material must be strong enough and durable to withstand the physiological loads placed upon it over the years. A suitable balance between strength and stiffness has to be found to the best match of bone behaviour. Titanium and its alloy (Ti6Al4V) have elastic moduli less than 50% of that commonly used Co–Cr implants so that their use helps reduce the extent of stress shielding. One consideration to achieve this has been the development of materials that exhibit substantial surface or total bulk porosity in medical applications. Moreover, bioactive surface chemical composition and suitable surface morphology on micro- and nanoscale level is necessary for efficient osseointegration. Here we report on novel concept of hierarchically functionalized titanium based biomaterials consists in (i) laser induced surface porosity, (ii) micro/messoporous bioactive glass fillers, (iii) chemical activation and/or ablative deposition of bioactive CaTiO3 nanoparticles. Prepared biomaterials were analyzed using SEM/EDX, XRD, Raman spectroscopy and test in terms of cell cultivation in order to reveal biocompatibility, cell viability and osseointegration ability.