1. Association between specific adipose tissue CD4+ T-cell populations and insulin resistance in obese individuals
- Author
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Elisa Fabbrini, Faidon Magkos, David Bradley, Zhouji Chen, J. Christopher Eagon, Steve A. Mccartney, Marina Cella, Caterina Conte, Samuel Klein, Dong Ho Han, Bruce W. Patterson, Gemma Fraterrigo, Nada A. Abumrad, Terri A. Pietka, Anja Fuchs, Brian N. Finck, Marco Colonna, Fabbrini, E, Cella, M, Mccartney, Sa, Fuchs, A, Abumrad, Na, Pietka, Ta, Chen, Z, Finck, Bn, Han, Dh, Magkos, F, Conte, Caterina, Bradley, D, Fraterrigo, G, Eagon, Jc, Patterson, Bw, Colonna, M, and Klein, S.
- Subjects
CD4-Positive T-Lymphocytes ,FFM ,nonalcoholic fatty liver disease ,Male ,medicine.medical_treatment ,Adipose tissue ,White adipose tissue ,Body Mass Index ,T-Lymphocyte Subsets ,Receptors ,Nonalcoholic fatty liver disease ,Hepatocyte ,Lymphocytes ,GIF ,Receptors, Interleukin-17 ,interleukin ,Interleukin-17 ,Gastroenterology ,interferon ,Skeletal ,Glucose clamp technique ,Middle Aged ,Cytokine ,medicine.anatomical_structure ,Liver ,metabolically normal insulin-sensitive obese ,CD4-Positive T-Lymphocyte ,MAO ,Cytokines ,metabolically abnormal insulin-resistant obese ,Muscle ,Lymphocyte ,Female ,Case-Control Studie ,Human ,Receptor ,Adult ,medicine.medical_specialty ,MNO ,Adipose tissue macrophages ,Subcutaneous Fat ,glucose infusion rate ,T-Lymphocyte Subset ,Biology ,fat free ma ,IFN ,Article ,Insulin resistance ,Internal medicine ,NAFLD ,medicine ,Animals ,Humans ,Obesity ,Muscle, Skeletal ,Hepatology ,Metabolically Abnormal Obesity ,Animal ,Interleukin-6 ,Interleukins ,c-Jun kinase ,Skeletal muscle ,Settore MED/13 - ENDOCRINOLOGIA ,IL ,Receptors, Interleukin ,medicine.disease ,Rats ,Endocrinology ,Glucose ,Case-Control Studies ,Hepatocytes ,Glucose Clamp Technique ,fat free mass ,Rat ,Th17 Cells ,JNK ,Insulin Resistance ,Metabolically Normal Obesity - Abstract
Background & Aims An increased number of macrophages in adipose tissue is associated with insulin resistance and metabolic dysfunction in obese people. However, little is known about other immune cells in adipose tissue from obese people, and whether they contribute to insulin resistance. We investigated the characteristics of T cells in adipose tissue from metabolically abnormal insulin-resistant obese (MAO) subjects, metabolically normal insulin-sensitive obese (MNO) subjects, and lean subjects. Insulin sensitivity was determined by using the hyperinsulinemic euglycemic clamp procedure. Methods We assessed plasma cytokine concentrations and subcutaneous adipose tissue CD4 + T-cell populations in 9 lean, 12 MNO, and 13 MAO subjects. Skeletal muscle and liver samples were collected from 19 additional obese patients undergoing bariatric surgery to determine the presence of selected cytokine receptors. Results Adipose tissue from MAO subjects had 3- to 10-fold increases in numbers of CD4 + T cells that produce interleukin (IL)-22 and IL-17 (a T-helper [Th] 17 and Th22 phenotype) compared with MNO and lean subjects. MAO subjects also had increased plasma concentrations of IL-22 and IL-6. Receptors for IL-17 and IL-22 were expressed in human liver and skeletal muscle samples. IL-17 and IL-22 inhibited uptake of glucose in skeletal muscle isolated from rats and reduced insulin sensitivity in cultured human hepatocytes. Conclusions Adipose tissue from MAO individuals contains increased numbers of Th17 and Th22 cells, which produce cytokines that cause metabolic dysfunction in liver and muscle in vitro. Additional studies are needed to determine whether these alterations in adipose tissue T cells contribute to the pathogenesis of insulin resistance in obese people.
- Published
- 2013