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266 results on '"Stepp, Mary Ann"'

4. Molecular mechanisms regulating wound repair: Evidence for paracrine signaling from corneal epithelial cells to fibroblasts and immune cells following transient epithelial cell treatment with Mitomycin C

Author

Pal-Ghosh, Sonali, Karpinski, Beverly A., Datta Majumdar, Himani, Ghosh, Trisha, Thomasian, Julie, Brooks, Stephen R., Sawaya, Andrew P., Morasso, Maria I., Scholand, Kaitlin K., de Paiva, Cintia S., Galletti, Jeremias G., and Stepp, Mary Ann

Published
2023
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16. Uveitis-mediated immune cell invasion through the extracellular matrix of the lens capsule

Author

DeDreu, JodiRae, Pal-Ghosh, Sonali, Mattapallil, Mary J, Caspi, Rachel R, Stepp, Mary Ann, Menko, A Sue, DeDreu, JodiRae, Pal-Ghosh, Sonali, Mattapallil, Mary J, Caspi, Rachel R, Stepp, Mary Ann, and Menko, A Sue

Abstract

While the eye is considered an immune privileged site, its privilege is abrogated when immune cells are recruited from the surrounding vasculature in response to trauma, infection, aging, and autoimmune diseases like uveitis. Here, we investigate whether in uveitis immune cells become associated with the lens capsule and compromise its privilege in studies of C57BL/6J mice with experimental autoimmune uveitis. These studies show that at D14, the peak of uveitis in these mice, T cells, macrophages, and Ly6G/Ly6C+ immune cells associate with the lens basement membrane capsule, burrow into the capsule matrix, and remain integrated with the capsule as immune resolution is occurring at D26. 3D surface rendering image analytics of confocal z-stacks and scanning electron microscopy imaging of the lens surface show the degradation of the lens capsule as these lens-associated immune cells integrate with and invade the lens capsule, with a subset infiltrating both epithelial and fiber cell regions of lens tissue, abrogating its immune privilege. Those immune cells that remain on the surface often become entwined with a fibrillar net-like structure. Immune cell invasion of the lens capsule in uveitis has not been described previously and may play a role in induction of lens and other eye pathologies associated with autoimmunity.

Published
2022

28. Identification of presumed corneal neuromas and microneuromas using laser-scanning in vivo confocal microscopy: a systematic review.

Author

Chinnery, Holly Rose, Rajan, Rajni, Jiao, Haihan, Wu, Mengliang, Zhang, Alexis Ceecee, Harshan De Silva, Manikkuwadura Eranda, Makrai, Eve, Stepp, Mary Ann, Di Girolamo, Nick, and Downie, Laura Elizabeth

Abstract

Background/aims This systematic review critically evaluated peer-reviewed publications describing morphological features consistent with, or using terms related to, a 'neuroma' or 'microneuroma' in the human cornea using laser-scanning in vivo confocal microscopy (IVCM). Methods The review was prospectively registered on PROSPERO (CRD42020160038). Comprehensive literature searches were performed in Ovid MEDLINE, Ovid Embase and the Cochrane Library in November 2019. The review included primary research studies and reviews that described laser-scanning IVCM for examining human corneal nerves. Papers had to include at least one of a pre-specified set of keyword stems, broadly related to neuromas and microneuromas, to describe a corneal nerve feature. Results Twenty-five papers (20 original studies; 5 reviews) were eligible. Three original studies evaluated corneal nerve features in healthy eyes. Most papers assessed corneal nerves in ocular and systemic conditions; seven studies did not include a control/comparator group. There was overlap in terminology used to describe nerve features in healthy and diseased corneas (eg, bulb-like/bulbous, penetration, end/s/ing). Inspection of IVCM images within the papers revealed that features termed 'neuromas' and 'microneuromas' could potentially be physiological corneal stromal-epithelial nerve penetration sites. We identified inconsistent definitions for terms, and limitations in IVCM image acquisition, sampling and/or reporting that may introduce bias and lead to inaccurate representation of physiological nerve characteristics as pathological. Conclusion These findings identify a need for consistent nomenclature and definitions, and rigorous IVCM scanning and analysis protocols to clarify the prevalence of physiological, as opposed to pathological, corneal nerve features. [ABSTRACT FROM AUTHOR]

Published
2022
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29. Type 2 immunity induced by bladder extracellular matrix enhances corneal wound healing

Author

Wang, Xiaokun, primary, Chung, Liam, additional, Hooks, Joshua, additional, Maestas, David R., additional, Lebid, Andriana, additional, Andorko, James I., additional, Huleihel, Luai, additional, Chin, Alexander F., additional, Wolf, Matthew, additional, Remlinger, Nathaniel T., additional, Stepp, Mary Ann, additional, Housseau, Franck, additional, and Elisseeff, Jennifer H., additional

Published
2021
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30. An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils.

Author

DeDreu, JodiRae, Bowen, Caitlin J, Logan, Caitlin M, Pal-Ghosh, Sonali, Parlanti, Paola, Stepp, Mary Ann, Menko, A Sue, DeDreu, JodiRae, Bowen, Caitlin J, Logan, Caitlin M, Pal-Ghosh, Sonali, Parlanti, Paola, Stepp, Mary Ann, and Menko, A Sue

Abstract

The lens and central cornea are avascular. It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril-associated protein-1 (MAGP1)-rich ciliary zonules that originate from the vasculature-rich ciliary body and extend along the surface of the lens capsule, form a potential conduit for immune cells to the lens. In response to cornea debridement wounding, we find increased expression of MAGP1 throughout the central corneal stroma. The immune cells that populate this typically avascular region after wounding closely associate with this MAGP1-rich matrix. These results suggest that MAGP1-rich microfibrils support immune cell migration post-injury. Using this cornea wound model, we investigated whether there is an immune response to the lens following cornea injury involving the lens-associated MAGP1-rich ciliary zonules. Our results provide the first evidence that following corneal wounding immune cells are activated to travel along zonule fibers that extend anteriorly along the equatorial surface of the lens, from where they migrate across the anterior lens capsule. These results demonstrate that lens-associated ciliary zonules are directly involved in the lens immune response and suggest the ciliary body as a source of immune cells to the avascular lens.

Published
2020

31. Identification of presumed corneal neuromas and microneuromas using laser-scanning in vivo confocal microscopy: a systematic review

Author

Chinnery, Holly Rose, primary, Rajan, Rajni, additional, Jiao, Haihan, additional, Wu, Mengliang, additional, Zhang, Alexis Ceecee, additional, De Silva, Manikkuwadura Eranda Harshan, additional, Makrai, Eve, additional, Stepp, Mary Ann, additional, Di Girolamo, Nick, additional, and Downie, Laura Elizabeth, additional

Published
2021
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32. Cover Image

Author

Bargagna‐Mohan, Paola, primary, Schultz, Gwendolyn, additional, Rheaume, Bruce, additional, Trakhtenberg, Ephraim F., additional, Robson, Paul, additional, Pal‐Ghosh, Sonali, additional, Stepp, Mary Ann, additional, Given, Katherine S., additional, Macklin, Wendy B., additional, and Mohan, Royce, additional

Published
2021
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48. Proteolytic processing of endogenous and recombinant beta-4 integrin subunits

Author

Giancoti, Filippo G., Stepp, Mary Ann, Suzuki, Shintaro, Engvall, Eva, and Ruoslahti, Erkki

Subjects
Proteolysis -- Physiological aspects, Cell receptors -- Analysis, Cell adhesion -- Molecular aspects, Biological sciences
Abstract

The alpha(sub 6)beta(sub 4) integrin is a receptor molecule involved in the adhesion of epithelial cells to the basement membrane. To study the role of this integrin, gene transfer experiments were performed to monitor the fate of the expressed proteins in stably-expressing cells. The results showed that the beta subunit of this integrin undergoes proteolytic processing. Similar experiments performed on corneal tissue did not reveal the products of proteolytic cleavage. These results indicate that the cleavage of the beta(sub 4) subunit is tissue specific, and may be significant in the regulation of this integrin's functions.

Published
1992

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