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16 results on '"Stephens, L.R."'

2. The G-beta-gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101

Author

Stephens, L.R., Eguinoa, A., Erdjument-Bromage, H., Lui, M., Cooke, F., Coadwell, J., Smrcka, A.S., Thelen, M., Cadwallader, K., Tempst, P., and Hawkins, P.T.

Subjects
Cell culture -- Usage, Genetic regulation -- Analysis, Cloning -- Analysis, Enzymatic analysis -- Usage, Cell receptors -- Research, Biological sciences
Abstract

Molecular biological techniques were used to characterize the activity of G-beta-gamma-sensitive PI3K using cultured Sf9 insect cells and U937 mammalian cells as host systems. The findings showed that p120 and p101 which are proteins that comprise PI3Ks bind tightly to each other, both in vivo and in vitro. p101 was observed to amplify the effect of G-beta-gamma on the catalytic activity of the PI3K's p120 component from less than 2-fold to greater than 100-fold.

Published
1997

5. Identification of ARAP3, a Novel PI3K Effector Regulating Both Arf and Rho GTPases, by Selective Capture on Phosphoinositide Affinity Matrices

Author

Krugmann, S., Anderson, K.E., Ridley, S.H., Risso, N., McGregor, A., Coadwell, J., Davidson, K., Eguinoa, A., Ellson, C.D., Lipp, P., Manifava, M., Ktistakis, N., Painter, G., Thuring, J.W., Cooper, M.A., Lim, Z.-Y., Holmes, A.B., Dove, S.K., Michell, R.H., Grewal, A., Nazarian, A., Erdjument-Bromage, H., Tempst, P., Stephens, L.R., and Hawkins, P.T.

Published
2002
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7. Platelet-derived growth factor stimulates synthesis of PtdIns(3,4,5)P3 by activating a PtdIns(4,5)P2 3-OH kinase

Author

Hawkins, P.T., Jackson, T.R., and Stephens, L.R.

Subjects
Phosphatidylinositol -- Physiological aspects, Platelet-derived growth factor -- Research, Protein kinases -- Research, Cellular signal transduction -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Platelet-derived growth factor (PDGF) initiates synthesis of 3-phosphorylated inositol lipids by using a phosphatidylinositol(4,5)-bisphoshate (3)-hydroxy (PtdIns4,5P2 3-OH) kinase. The previous discovery of a very similar mechanism for the release of neutrophils by the formyl-peptide fMet-Leu-Phe indicates that the main function of both PDGF and formyl-peptide is to trigger synthesis of PtdIns(3,4,5)P3 by activating PtdIns(45)P2-selective 3-OH kinase. These reactions may constitute a cellular signalling system.

Published
1992

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