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169 results on '"Stehle, Jean-Christophe"'

1. Data from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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2. Supplementary Figure 4 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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3. Supplementary Figure 7 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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4. Supplementary Figure Legend from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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5. Supplementary Figure 1 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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6. Supplementary Figure 8 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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7. Supplementary Figure 2 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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8. Supplementary Figure 5 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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9. Supplementary Figure 6 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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10. Supplementary Figure 3 from Inhibition of the Kit Ligand/c-Kit Axis Attenuates Metastasis in a Mouse Model Mimicking Local Breast Cancer Relapse after Radiotherapy

Author

Kuonen, François, primary, Laurent, Julien, primary, Secondini, Chiara, primary, Lorusso, Girieca, primary, Stehle, Jean-Christophe, primary, Rausch, Thierry, primary, Faes-van't Hull, Eveline, primary, Bieler, Grégory, primary, Alghisi, Gian-Carlo, primary, Schwendener, Reto, primary, Andrejevic-Blant, Snezana, primary, Mirimanoff, René-Olivier, primary, and Rüegg, Curzio, primary

Published
2023
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12. Supplementary Figure 1 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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13. Supplementary Table 1 from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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14. Supplementary Figure 3 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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16. Supplementary Figure 2 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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17. Data from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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18. Supplementary Figure 1 from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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19. Data from EZH2 Is Essential for Glioblastoma Cancer Stem Cell Maintenance

Author

Suvà, Mario-Luca, primary, Riggi, Nicolò, primary, Janiszewska, Michalina, primary, Radovanovic, Ivan, primary, Provero, Paolo, primary, Stehle, Jean-Christophe, primary, Baumer, Karine, primary, Le Bitoux, Marie-Aude, primary, Marino, Denis, primary, Cironi, Luisa, primary, Marquez, Victor E., primary, Clément, Virginie, primary, and Stamenkovic, Ivan, primary

Published
2023
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20. Supplementary Figure 2 from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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21. Data from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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23. Supplementary Methods and Materials from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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25. Supplementary Figure 3 from CYR61 and αVβ5 Integrin Cooperate to Promote Invasion and Metastasis of Tumors Growing in Preirradiated Stroma

Author

Monnier, Yan, primary, Farmer, Pierre, primary, Bieler, Gregory, primary, Imaizumi, Natsuko, primary, Sengstag, Thierry, primary, Alghisi, Gian Carlo, primary, Stehle, Jean-Christophe, primary, Ciarloni, Laura, primary, Andrejevic-Blant, Snezana, primary, Moeckli, Raphael, primary, Mirimanoff, René-Olivier, primary, Goodman, Simon L., primary, Delorenzi, Mauro, primary, and Rüegg, Curzio, primary

Published
2023
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26. Supplementary Data 2 from EZH2 Is Essential for Glioblastoma Cancer Stem Cell Maintenance

Author

Suvà, Mario-Luca, primary, Riggi, Nicolò, primary, Janiszewska, Michalina, primary, Radovanovic, Ivan, primary, Provero, Paolo, primary, Stehle, Jean-Christophe, primary, Baumer, Karine, primary, Le Bitoux, Marie-Aude, primary, Marino, Denis, primary, Cironi, Luisa, primary, Marquez, Victor E., primary, Clément, Virginie, primary, and Stamenkovic, Ivan, primary

Published
2023
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27. Supplementary Figure 4 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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30. Supplementary Table 1 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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31. Supplementary Table 2 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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32. Supplementary Data Legends 1-3 from EWS-FLI-1 Expression Triggers a Ewing's Sarcoma Initiation Program in Primary Human Mesenchymal Stem Cells

Author

Riggi, Nicolò, primary, Suvà, Mario-Luca, primary, Suvà, Domizio, primary, Cironi, Luisa, primary, Provero, Paolo, primary, Tercier, Stéphane, primary, Joseph, Jean-Marc, primary, Stehle, Jean-Christophe, primary, Baumer, Karine, primary, Kindler, Vincent, primary, and Stamenkovic, Ivan, primary

Published
2023
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34. Supplementary Figure Legend 1 from Bcl9/Bcl9l Are Critical for Wnt-Mediated Regulation of Stem Cell Traits in Colon Epithelium and Adenocarcinomas

Author

Deka, Jürgen, primary, Wiedemann, Norbert, primary, Anderle, Pascale, primary, Murphy-Seiler, Fabienne, primary, Bultinck, Jennyfer, primary, Eyckerman, Sven, primary, Stehle, Jean-Christophe, primary, André, Sylvie, primary, Vilain, Nathalie, primary, Zilian, Olav, primary, Robine, Sylvie, primary, Delorenzi, Mauro, primary, Basler, Konrad, primary, and Aguet, Michel, primary

Published
2023
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35. Supplementary Data 1 from EZH2 Is Essential for Glioblastoma Cancer Stem Cell Maintenance

Author

Suvà, Mario-Luca, primary, Riggi, Nicolò, primary, Janiszewska, Michalina, primary, Radovanovic, Ivan, primary, Provero, Paolo, primary, Stehle, Jean-Christophe, primary, Baumer, Karine, primary, Le Bitoux, Marie-Aude, primary, Marino, Denis, primary, Cironi, Luisa, primary, Marquez, Victor E., primary, Clément, Virginie, primary, and Stamenkovic, Ivan, primary

Published
2023
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39. Myeloid-derived suppressor cells are implicated in regulating permissiveness for tumor metastasis during mouse gestation

Author

Mauti, Laetitia A., Bitoux, Marie-Aude Le, Baumer, Karine, Stehle, Jean-Christophe, Golshayan, Dela, Provero, Paolo, and Stamenkovic, Ivan

Subjects
Physiological aspects, Research, Genetic aspects, Killer cells -- Physiological aspects -- Research, Pregnancy -- Physiological aspects -- Research -- Genetic aspects, Cancer metastasis -- Research -- Genetic aspects, Gene expression -- Research, Metastasis -- Research -- Genetic aspects
Abstract

Metastasis depends on the ability of tumor cells to establish a relationship with the newly seeded tissue that is conducive to their survival and proliferation. However, the factors that render [...]

Published
2011
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41. The Glucocorticoid-Induced Leucine Zipper (Gilz/Tsc22d3-2) Gene Locus Plays a Crucial Role in Male Fertility

Author

Suarez, Philippe Emmanuel, Rodriguez, Elena Gonzalez, Soundararajan, Rama, Mérillat, Anne-Marie, Stehle, Jean-Christophe, Rotman, Samuel, Roger, Thierry, Voirol, Marie-Jeanne, Wang, Jian, Gross, Olaf, Pétrilli, Virginie, Nadra, Karim, Wilson, Anne, Beermann, Friedrich, Pralong, François Pierre, Maillard, Marc, Pearce, David, Chrast, Roman, Rossier, Bernard Claude, and Hummler, Edith

Published
2012

46. Immunohistochemical localization of hTERT protein in human tissues

Author

Yan, Pu, Benhattar, Jean, Seelentag, Walter, Stehle, Jean-Christophe, Bosman, Fred, Yan, Pu, Benhattar, Jean, Seelentag, Walter, Stehle, Jean-Christophe, and Bosman, Fred

Abstract

Telomerase is a ribonucleoprotein complex mainly composed of a reverse transcriptase catalytic subunit (telomerase reverse transcriptase gene, hTERT) that copies a template region of its RNA subunit to the end of the telomere. For detecting telomerase activity in a tissue specimen the TRAP assay is a relatively sensitive and specific method, but it can be used only on fresh tissue extracts and offers no information at the single cell level. Immunohistochemistry (IHC) allows to detect hTERT protein expression at an individual cell level in human tissues. We have tested commercially available anti-hTERT antibodies in formalin-fixed and paraffin-embedded human tissues by IHC. Only one monoclonal antibody (NCL-hTERT; Novacastra) was sufficiently specific and this was applied to human tissues in which telomerase activity had been shown by TRAP assay and hTERT mRNA expression by RT-PCR. hTERT protein localized diffusely in the nucleoplasm and more intensely in the nucleoli of cancer cells and proliferating normal cells. Mitotic cells showed diffuse staining of the entire cell. Granular cytoplasmic staining was occasionally found in some tumor cells. In telomerase-positive tumors not all the tumor cells showed hTERT immunoreactivity. A significantly heterogeneous hTERT protein expression was observed in human tumor tissues. The hTERT immunostaining in fixed tissues was concordant with telomerase activity and hTERT mRNA expression in corresponding non-fixed samples. Quantitative RT-PCR of microdissected sections showed that hTERT mRNA expression was higher in cells with nuclear expression than in those with cytoplasmic expression. Double staining with the M30 antibody showed that a subpopulation of hTERT-negative cells is apoptotic. We conclude that: (1) hTERT protein can be detected by IHC in fixed human tissues, but the choice of the antibody, tissue processing, and reaction conditions are critical, (2) hTERT protein localizes in the nucleoplasm, more strongly in the nucl

Published
2018

47. EWS-FLI-1 modulates miRNA145 and SOX2 expression to initiate mesenchymal stem cell reprogramming toward Ewing sarcoma cancer stem cells

Author

Riggi, Nicolo, Suva, Mario-Luca, Vito, Claudio De, Provero, Paolo, Stehle, Jean-Christophe, Baumer, Karine, Cironi, Luisa, Janiszewska, Michalina, Petricevic, Tanja, Suva, Domizio, Tercier, Stephane, Joseph, Jean-Marc, Guillou, Louis, and Stamenkovic, Ivan

Subjects
Embryonic stem cells -- Research, Ewing's sarcoma -- Genetic aspects, Gene expression -- Analysis, Biological sciences
Published
2010

48. Acidic tumor microenvironment abrogates the efficacy of mTORC1 inhibitors

Author

Faes, Seraina, primary, Duval, Adrian P., additional, Planche, Anne, additional, Uldry, Emilie, additional, Santoro, Tania, additional, Pythoud, Catherine, additional, Stehle, Jean-Christophe, additional, Horlbeck, Janine, additional, Letovanec, Igor, additional, Riggi, Nicolo, additional, Demartines, Nicolas, additional, and Dormond, Olivier, additional

Published
2016
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50. The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation

Author

Hummler Edith, Dousse Aline, Rieder Audrey, Stehle Jean Christophe, Rubera Isabelle, Osterheld Maria Chiara, Beermann Friedrich, Frateschi Simona, and Charles Roch Philippe

Subjects
Male, Embryology, Cellular differentiation, Gene Expression, Cardiovascular, Biochemistry, Mice, 0302 clinical medicine, Pregnancy, Molecular Cell Biology, Morphogenesis, Mice, Knockout, 0303 health sciences, Multidisciplinary, Cell Death, Serine Endopeptidases, Gene Expression Regulation, Developmental, Embryo, Cell Differentiation, 3. Good health, Cell biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, embryonic structures, Embryo Loss, Medicine, Female, Research Article, Proteases, Science, Embryonic Development, 610 Medicine & health, Placental insufficiency, Biology, Real-Time Polymerase Chain Reaction, Cell Growth, 03 medical and health sciences, Vascular Biology, Placenta, medicine, Animals, RNA, Messenger, Gene knockout, 030304 developmental biology, Gene Expression Profiling, Embryogenesis, Placentation, Proteins, Molecular Development, medicine.disease, Embryo, Mammalian, Transmembrane Proteins, Animals, Newborn, Ear, Inner, Immunology, 570 Life sciences, biology, Organism Development, Developmental Biology
Abstract

The serine protease CAP1/Prss8 is crucial for skin barrier function lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects but it is during this period and in particular at E13.5 that wildtype placentas show an increased expression of CAP1/Prss8 thus suggesting a placental defect in the knockout situation. The placentas of knockout embryos exhibited significantly reduced vascular development and incomplete cellular maturation. In contrary epiblast specific deletion of CAP1/Prss8 allowed development until birth. These CAP1/Prss8 deficient newborns presented abnormal epidermis and died soon after birth due to impaired skin function. We thus conclude that a late placental insufficiency might be the primary cause of embryonic lethality in CAP1/Prss8 knockouts. This study highlights a novel and crucial role for CAP1/Prss8 in placental development and function. © 2013 Hummler et al.

Published
2013
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