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4. Autophagy upregulation may explain inhibition of oral carcinoma in situ by photobiomodulation in vitro.

Author

Stefenon L, Boasquevisque M, Garcez AS, de Araújo VC, Soares AB, Santos-Silva AR, Sperandio F, Brod JMM, and Sperandio M

Subjects
Beclin-1 genetics, Beclin-1 metabolism, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Cell Line, Coculture Techniques, Endoplasmic Reticulum Chaperone BiP, Humans, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, RNA, Messenger metabolism, Autophagy genetics, Light, Up-Regulation radiation effects
Abstract

There is currently no clear understanding on the pathways involved in the process of cell inhibition by photobiomodulation (PBM). The present study evaluated the influence of PBM on the expression of autophagy markers in vitro in an in situ model of oral carcinoma. Oral squamous cell carcinoma (Cal27) and stromal fibroblasts (FG) cultures were used. The independent variables were 'cell type' (FG and CAL27) 'culture condition' (monocultures or co-cultures) and PBM (placebo and 36 J/cm 2 ). The cultures were irradiated from a red LED source for mRNA expression and protein expression analyses. The autophagy markers evaluated were Beclin-1, LC3B and p62 as well as adjuvant markers (BAX Bcl-2, VEGF, CD105, CD34, PRDX1, PRDX4 and GRP78). The Cal27 cells upregulated the autophagy markers upon exposure to PBM both at the mRNA and protein expression levels, providing evidence to explain malignant cell inhibition by PBM., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Published
2021
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