19 results on '"Stefano Pignalosa"'
Search Results
2. Serum Neurofilament and Free Light Chain Levels in Patients Undergoing Treatment for Chronic Inflammatory Demyelinating Polyneuropathy
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Marco Luigetti, Guido Primiano, Valerio Basile, Francesca Vitali, Stefano Pignalosa, Angela Romano, Andrea Sabino, Mariapaola Marino, Riccardo Di Santo, Gabriele Ciasca, and Umberto Basile
- Subjects
chronic inflammatory demyelinating polyneuropathy ,biomarkers ,neurofilament light chain ,free light chains ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an immune-mediated disorder affecting the peripheral nervous system. Despite the established diagnostic criteria, monitoring disease activity and treatment remains challenging. To address this limitation, we investigated serum neurofilament light chain (sNfL) and serum free light chains (sFLCs) as potential biomarkers. A total of 32 CIDP patients undergoing immunoglobulin therapy and 32 healthy controls enrolled in the present study, and agreed to have their blood plasma sNfL and sFLCs analyzed, while CIDP severity was assessed through the modified Rankin Scale (mRS) and the Overall Neuropathy Limitations Scale (ONLS). In line with the immunoglobulin treatment aimed at limiting neuronal damage administered to the majority of patients, sNfL levels did not exhibit significant differences between the two groups. However, CIDP patients showed significantly elevated sFLC and sFLC ratios, while the marker levels did not correlate with the clinical scores. The study confirms the potential of sFLCs as a sensitive biomarker of inflammatory processes in CIDP. Additionally, the present study results regarding neurofilaments strengthen the role of sNfL in monitoring CIDP treatments, confirming the effectiveness of immunoglobulin therapy. Overall, our results demonstrate how combining these markers can lead to better patient characterization for improved treatment.
- Published
- 2024
- Full Text
- View/download PDF
3. Molecular Characterization of Whole-Genome SARS-CoV-2 from the First Suspected Cases of the XE Variant in the Lazio Region, Italy
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Martina Rueca, Emanuela Giombini, Giulia Gramigna, Cesare Ernesto Maria Gruber, Lavinia Fabeni, Angela Corpolongo, Valentina Mazzotta, Luisella Corso, Ornella Butera, Maria Beatrice Valli, Fabrizio Carletti, Stefano Pignalosa, Francesco Vairo, Emanuele Nicastri, Andrea Antinori, Enrico Girardi, Francesco Vaia, Fabrizio Maggi, and SARS CoV-2 Lazio Surveillance Study Group
- Subjects
COVID-19 ,SARS-CoV-2 ,whole-genome sequencing ,recombination ,PANGOLIN ,Nextclade ,Medicine (General) ,R5-920 - Abstract
We report two cases of SARS-CoV-2 recombinant variant XE detected in nasopharyngeal swabs (NPS) of hospitalized patients with no evident epidemiological link in Lazio, Central Italy. Whole-Genome Sequencing (WGS) performed on an Ion Torrent GSS5 platform according to Italian flash surveys showed genomes corresponding to the PANGOLIN unclassified lineage and the Nextclade XE clade. Further analyses were then carried out to investigate more deeply the genetic characteristics of these XE-like sequences. When phylogenetic trees, by using IQ-TREE, were built splitting the genome into two regions according to the putative XE recombination site, the upstream and downstream regions were seen to be clustered near BA.1 and BA.2 sequences, respectively. However, our XE-like sequences clustered separately, with a significant bootstrap, from the classified European and Italian XE strains, although the recombination site between BA.1 and BA.2 was identified at the nucleotide site 11556 by RDP4 software, consistent with the putative XE breakpoint. These findings show the risk of the introduction of novel recombinant variants of SARS-CoV-2 and the existence of XE-like strains, phylogenetically separated, that could make their exact taxonomy difficult. It follows the need for continued SARS-CoV-2 surveillance by WGS.
- Published
- 2022
- Full Text
- View/download PDF
4. Prevalence of major cardiovascular risk factors among oil and gas and energy company workers
- Author
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Alice Mannocci, Stefano Pignalosa, Rosella Saulle, Sabina Sernia, Sabatino De Sanctis, Massimo Consentino, Cesare Gialdi, Vincenzo Nicosia, and Giuseppe La Torre
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cardiovascular risk ,dyslipidemia ,hypertension ,oil company workers ,Public aspects of medicine ,RA1-1270 - Abstract
INTRODUCTION: Cardiovascular diseases (CVD) remain the biggest cause of disability and premature death throughout the world. AIM: The aim of this study was to describe and determine the prevalence of major cardiovascular risk factors emerged at the first medical examination carried out by a group of an oil and gas contractor company workers in the observation period 2000-2010. METHODS: An observational cross-sectional study was conducted on 1073 workers (mean age 41 years, SD = 9.5) presenting overweight BMI (body mass index) values, hypertension and cholesterol problems. RESULTS: In particular, we found that workers > 45 years had significant higher risk to have obesity (OR = 3.8, CI 95% = 2.5-5.7), hypertension (OR = 2.7, CI 95% = 2.1-3.6), high blood fasting glucose (OR = 2.6, CI 95% = 1.2-5.5), high cholesterol (OR = 2.7, CI 95% = 2.0-3.6), high triglycerides (OR = 1.8, CI 95% = 1.4-2.4) compared to younger (< 45 years).
- Published
- 2015
- Full Text
- View/download PDF
5. Laboratory and Clinical Settings of Heavy/Light Chain (HLC) Assays in the Management of Monoclonal Gammopathies and Multiple Myeloma
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Cecilia Napodano, Laura Ioannilli, Valerio Basile, Francesca Gulli, Valeria Carnazzo, Stefano Pignalosa, Luigi Di Biase, Erica Cavaleri, Cosimo Racco, Francesco Equitani, Mariapaola Marino, and Umberto Basile
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multiple myeloma ,heavy/light chain ,Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA ,monoclonal component ,Medicine (miscellaneous) ,free light chain ,Settore MED/05 - PATOLOGIA CLINICA - Abstract
The antibody-related immune response is mediated by immunoglobulins (Igs), soluble circulating glycoproteins produced by activated B cells that, upon the recognition of specific epitopes on pathogen surfaces, activate, proliferate, and differentiate into antibody-secreting plasma cells. Although the antibodies are effectors of the humoral immune adaptive response, their overproduction in response to a dysregulated proliferation of clonal plasma cell production in tumoral conditions (i.e., multiple myeloma), enriches the serum and urinary matrices, assuming the crucial role of biomarkers. Multiple myeloma (MM) is a plasma cell dyscrasia characterized by the expansion and accumulation of clonally activated plasma cells in bone marrow, determining the release of high amounts of monoclonal component (MC) that can be detected as intact immunoglobulin (Ig), immunoglobulin fragments, or free light chains (FLCs). The importance of detecting biomarkers for the diagnosis, monitoring, and prognosis of diseases is highlighted by the international guidelines that recommend specific assays for the analysis of intact Igs and FLC. Moreover, a developed assay called Hevylite® allows for the quantification of immunoglobulins that are both involved (iHLC) and not involved (uHLC) in the tumor process; this is a fundamental aspect of following up the patient’s workup and evaluating the progression of disease, together with the treatments response. We here summarize the major points of the complex scenario involving monoclonal gammopathies and MM clinical management in view of advantages derived for the use of Hevylite®.
- Published
- 2023
6. Serum Immunoglobulin G (IgG) Subclasses in a Cohort of Systemic Sclerosis Patients
- Author
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Chiara Pellicano, Amalia Colalillo, Giuseppina Cusano, Andrea Palladino, Marica Pellegrini, Cinzia Anna Maria Callà, Giorgia Mazzuccato, Valeria Carnazzo, Stefano Pignalosa, Luigi Di Biase, Mariapaola Marino, Umberto Basile, and Edoardo Rosato
- Subjects
systemic sclerosis ,Medicine (miscellaneous) ,IgG subclasses ,ILD ,Settore MED/05 - PATOLOGIA CLINICA - Abstract
Objectives: To assess serum immunoglobulin G (IgG) subclasses in a cohort of systemic sclerosis (SSc) patients and to evaluate the influence of IgG subclasses in the main complications of the disease. Methods: The serum level of IgG subclasses was evaluated in 67 SSc patients and 48 healthy controls (HC), matched for sex and age. Serum samples were collected and measured IgG1–4 subclasses by turbidimetry. Results: SSc patients had lower median total IgG [9.88 g/l (IQR 8.18–11.42 g/l) vs. 12.09 g/l (IQR 10.24–13.54 g/l), p < 0.001], IgG1 [5.09 g/l (IQR 4.25–6.38 g/l) vs. 6.03 g/l (IQR 5.39–7.90 g/l), p < 0.001], and IgG3 [0.59 g/l (IQR 0.40–0.77 g/l) vs. 0.80 g/l (IQR 0.46–1 g/l), p < 0.05] serum levels compared to HC. The logistic regression analysis showed IgG3 as the only variable associated with the diffusing capacity of the lung for carbon monoxide (DLco) ≤60% of the predicted [OR 9.734 (CI 95%: 1.312–72.221), p < 0.05] and modified Rodnan skin score (mRSS) [OR 1.124 (CI 95%: 1.019–1.240), p < 0.05], anti-topoisomerase I [OR 0.060 (CI 95%: 0.007–0.535), p < 0.05], and IgG3 [OR 14.062 (CI 95%: 1.352–146.229), p < 0.05] as variables associated with radiological interstitial lung disease (ILD). Conclusion: SSc patients have reduced levels of total IgG and an altered IgG subclass distribution compared to HC. Moreover, SSc patients show different serum IgG subclasses profiles according to the main involvement of the disease.
- Published
- 2023
7. Serum iPTH range in a reference population: From an integrated approach to vitamin D prevalence impact evaluation
- Author
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Vincenzo Rossi, Daniela Bajo, Alessia Agnoli, Sergio Bernardini, Anna Maria De Angelis, Alessandro Terrinoni, Lorenza Bellincampi, Massimo Pieri, Stefano Pignalosa, Francesco Equitani, Filippa Valente, Bruno Daniele Leoni, and Marilena Minieri
- Subjects
0301 basic medicine ,Vitamin ,Male ,medicine.medical_specialty ,Percentile ,iPTH ,Clinical Biochemistry ,Population ,Biochemistry ,Gastroenterology ,vitamin D deficiency ,Reference interval ,LIMS data ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Vitamin D and neurology ,Prevalence ,Humans ,Vitamin D ,education ,education.field_of_study ,Creatinine ,business.industry ,Settore BIO/12 ,Biochemistry (medical) ,General Medicine ,medicine.disease ,Vitamin D Deficiency ,Secondary hyperparathyroidism ,030104 developmental biology ,chemistry ,Parathyroid Hormone ,030220 oncology & carcinogenesis ,Cohort ,Calcium ,Female ,business - Abstract
The iPTH upper reference limit (URL) reported by our laboratory provider (Abbott Laboratories) at Tor Vergata University Hospital was evaluated by internal verification procedures as not representative of our population and resulting as underestimated. In this study, a new reference interval has been investigated and established by comparing a direct and an indirect method based on a statistical reduction from results stored in the laboratory database.For reference interval calculation from the healthy population, we analyzed a cohort of 100 blood donors (84% males and 16% females) screened with no bone-related and malabsorption diseases. We analyzed a cohort of 495 patients retrieved from more than 800 iPTH results by excluding subjects with pathological measurement for calcium, phosphorus, and creatinine for the reference interval evaluation. Patients with vitamin D results were included in the analysis. Vitamin D sufficiency status during the period from January to September 2020 was also evaluated by investigating 3,050 patients.The iPTH reference interval of a healthy blood donor population was measured as 25.2-109.1 pg/mL (2.7-11.6 pmol/L) at 2.5 and 97.5 distribution percentile. The iPTH reference interval from data stored in the laboratory database was 19.3-112.5 pg/mL (2.0-11.9 pmol/L). Furthermore, 60% of the whole population had prevalently insufficient vitamin D concentration (30 ng/dL;75 nmol/L). The impact of vitamin D concentration on the iPTH reference interval was measured for insufficient vitamin D (30 ng/dL;75 nmol/L) as 15.2-127.7 pg/mL (1.6-13.5 pmol/L), desirable vitamin D (30-40 ng/ml; 75-100 nmol/L) as 25.6-105 pg/mL (2.7-10.7 pmol/L) and optimal vitamin D (40 ng/ml;100 nmol/L) as 26.2-89.2 pg/mL (2.8-9.4 pmol/L), respectively.The URL reported in manufacturer datasheets likely refers to a normal population with non-pathological vitamin D levels. On the contrary, the considered population was mostly vitamin D insufficient, resulting in a URL shift. On this basis, we suggest describing in medical reports the iPTH range for vitamin D deficiency for diagnosis of primary hyperparathyroidism even when a specific vitamin D request is lacking. On the other hand, reporting optimal vitamin D-based iPTH reference interval could be clinically relevant in supplemented patients as a marker of treatment efficacy.
- Published
- 2021
8. Evaluation of the Diesse Cube 30 touch erythrocyte sedimentation method in comparison with Alifax test 1 and the manual Westergren gold standard method
- Author
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Carmelo Russo, Andrea Perrone, Marco Alfonso Perrone, Massimo Pieri, Sergio Bernardini, Gianluca Noce, Alessandro Terrinoni, Stefano Pignalosa, and Renato Massoud
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Male ,Materials science ,Clinical Biochemistry ,Blood Sedimentation ,030204 cardiovascular system & hematology ,Erythrocyte sedimentation rate ,03 medical and health sciences ,Hemoglobins ,0302 clinical medicine ,Westergren method ,medicine ,Humans ,ESR ,evaluation ,medicine.diagnostic_test ,Erythrocyte sedimentation ,Settore BIO/12 ,General Medicine ,Gold standard (test) ,Laboratory test ,Hematocrit ,comparison ,030220 oncology & carcinogenesis ,Regression Analysis ,Female ,Cube ,Biomedical engineering - Abstract
The erythrocyte sedimentation rate (ESR) is a traditional nonspecific laboratory test used for the assessment of inflammation. Even if its usefulness is nowadays being largely debated, it is still considered a valuable laboratory test in selected clinical conditions, such as rheumatoid diseases, orthopedic infections and Hodgkin's lymphoma, and it can be used for the infectious, inflammatory, malignancies, and autoimmune diseases follow-up. The introduction of new methodologies on semi-automated and automated analyzers started about four decades ago and opened a new era of ESR analysis characterized by shorter assay time, use of (EDTA) undiluted blood, that increases sample stability and allows using a single sample for also other hematologic tests, and greater safety for laboratory personnel. In this context, the aim of this study was to evaluate the performances of new device Diesse Cube 30 touch, comparing it with Alifax Test 1 and with the gold standard Westergren method. The new Diesse Cube 30 touch for determination of the ESR shows a good correlation with the manual Westergren gold standard method in a shorter time, and in a standardized way, since all the phases of the test are automatized. The Diesse Cube 30 touch respect the manual gold standard method, displayed a small bias to confirm that the new automated test system tended to have a small bias for ESR values (mean positive bias +0.2 mm/h). The findings of the present study show that the Diesse Cube 30 touch Westergren-based method can be a valid alternative in laboratory analysis for the determination of ESR.
- Published
- 2021
9. New HPLC instrument performance evaluation in HbA1c determination and comparison with capillary electrophoresis
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Massimo Pieri, Sergio Bernardini, Carmelo Russo, Stefano Pignalosa, and Haneen A. Khashoggi
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030213 general clinical medicine ,Reproducibility ,Chromatography ,Correlation coefficient ,medicine.diagnostic_test ,Chemistry ,Settore BIO/12 ,Clinical Biochemistry ,General Medicine ,Repeatability ,030204 cardiovascular system & hematology ,University hospital ,High-performance liquid chromatography ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Capillary electrophoresis ,Immunoassay ,medicine ,Glycated hemoglobin - Abstract
Glycated hemoglobin (HbA1c) measurement provides the most important medium to long-term marker of time-averaged glycemic status. Its relationship to clinical outcome in diabetes has been convincingly demonstrated for both type 1 and type 2 diabetes. The main HbA1c measurement methods for clinical routine are as follows: ion-exchange chromatography; affinity chromatography, capillary electrophoresis, immunoassay and enzymatic methods. In this study, we evaluated the analytical performances of a new HPLC instrument (Tosoh HLC-723 G11 in the VAR mode) in HbA1c analysis and compared it with a capillary electrophoresis instrument (Sebia Capillarys 2 Flex Piercing). HbA1c analysis was performed in parallel by both methods for 250 samples randomly chosen from healthy and diabetic subjects at 'Tor Vergata' University Hospital of Rome. Tosoh HLC-723 G11 showed good reproducibility for 10 days both in quality controls and in samples analyzed (%CV
- Published
- 2018
10. HbA1c: Gender Differences in Non-Diabetic Young Population
- Author
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Annalisa Noce, Massimo Pieri, Fg Martino, Giuseppina Nocca, Zenobi, Sergio Bernardini, Cinzia Anna Maria Calla, N. Di Daniele, Giulia Marrone, Mariarita Dessì, and Stefano Pignalosa
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diabetes ,business.industry ,Ethnic group ,Type 2 Diabetes Mellitus ,General Medicine ,Overweight ,Race (biology) ,Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA ,medicine ,medicine.symptom ,business ,Body mass index ,Demography ,Non diabetic - Published
- 2019
11. Reference intervals for HbA1c partitioned for gender and age: a multicenter study
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Giuseppina Nocca, Giacomo Menichella, N. Di Daniele, Cinzia Anna Maria Calla, Michelle Pieri, Haneen A. Khashoggi, Sergio Bernardini, F Mancina, Rossella Zenobi, Umberto Moscato, Francesca Gabriela Martino, Mariarita Dessì, and Stefano Pignalosa
- Subjects
Glycated haemoglobin (HbA1c) ,Pediatrics ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,Age and gender ,Diabetes ,Gender medicine ,03 medical and health sciences ,Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA ,0302 clinical medicine ,Endocrinology ,Diabetes mellitus ,Internal Medicine ,medicine ,business.industry ,Settore BIO/12 ,General Medicine ,medicine.disease ,Reference intervals ,Surgery ,Multicenter study ,business - Published
- 2016
12. New HPLC instrument performance evaluation in HbA
- Author
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Haneen, Khashoggi, Stefano, Pignalosa, Carmelo, Russo, Massimo, Pieri, and Sergio, Bernardini
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Glycated Hemoglobin ,Diabetes Mellitus, Type 1 ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,Electrophoresis, Capillary ,Humans ,Reproducibility of Results ,Biomarkers ,Chromatography, High Pressure Liquid - Abstract
Glycated hemoglobin (HbA
- Published
- 2018
13. KFLC Index utility in multiple sclerosis diagnosis: Further confirmation
- Author
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Marianna Storto, Rossella Zenobi, Diego Centonze, Fabio Buttari, Stefano Pignalosa, Sergio Bernardini, Mariarita Dessì, and Massimo Pieri
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Pathology ,Index (economics) ,Immunology ,Cerebrospinal fluid ,Free light chain ,Multiple sclerosis ,Nephelometric assay ,kFLC Index ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Immunology and Allergy ,medicine.diagnostic_test ,business.industry ,Oligoclonal Bands ,Magnetic resonance imaging ,medicine.disease ,Magnetic Resonance Imaging ,Free Light Chain ,030104 developmental biology ,Neurology ,Immunoglobulin Light Chains ,Settore MED/26 - Neurologia ,Neurology (clinical) ,Radiology ,Nervous System Diseases ,business ,Biomarkers ,030217 neurology & neurosurgery - Abstract
The Multiple Sclerosis (MS) diagnosis is based on dissemination of focal lesions in time and space. The free light chains (FLCs) determination might be a sensitive alternative to oligoclonal bands assay. The study aim was to redefine sensitivity, specificity of the kFLC Index cut-off. We analyzed serum and cerebrospinal fluid of 176 patients, with different neurological disorders. We obtained a cut off of 12,3 for kFLC Index with a sensitivity and specificity of 93% and 100% respectively. Our data confirm that the kFLC Index is a valid tool in the diagnosis of MS.
- Published
- 2017
14. Levamisole in Illicit Trafficking Cocaine Seized: A One-Year Study
- Author
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Francesca Vernich, Simona Martello, Stefano Pignalosa, Cristiano Ialongo, Sergio Bernardini, Carmelo Russo, Gianluca Noce, Luigi Tonino Marsella, Massimo Pieri, and Federico Mineo
- Subjects
Lidocaine ,Medicine (miscellaneous) ,cocaine ,Pharmacology ,01 natural sciences ,Risk Assessment ,Gas Chromatography-Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adulterants ,Medicine ,Humans ,030216 legal & forensic medicine ,Drug Trafficking ,adulterants ,levamisole ,mass spectrometry ,General Psychology ,Leukopenia ,business.industry ,Settore BIO/12 ,010401 analytical chemistry ,Levamisole ,Reference Standards ,medicine.disease ,0104 chemical sciences ,Acetaminophen ,chemistry ,Phenacetin ,Anesthesia ,Calibration ,Aminorex ,Central Nervous System Stimulants ,Pulmonary hemorrhage ,medicine.symptom ,business ,Caffeine ,Drug Contamination ,medicine.drug - Abstract
Cocaine use is increasing around the world and its purity is frequently altered through dilution, substitution, contamination, and adulteration. Sugars, talc, starch, and carbonates represent the principal diluents of cocaine, while phenacetin, levamisole, caffeine, and lidocaine are its major adulterants in Europe. Levamisole is used because it is an odorless powder, with physical properties similar to cocaine, and it has reasonable cost and availability, being widely used in veterinary medicine. For this study, we analyzed 88 cocaine samples. The seized cocaine analyzed showed an average purity of 55% and the most frequent adulterants identified were: levamisole (31.8%), caffeine (6.8%), lidocaine (2.3%), acetaminophen (2.3%), and phenacetin (1.1%). Our aim is the study of the presence of levamisole, over other adulterants in seized cocaine samples, due to its recognized human toxicity. The chronic use of levamisole-adulterated cocaine represents a serious public health issue because it may be responsible for side-effects such as dermal vasculopathy, leukoencephalopathy, leukopenia, agranulocytosis, pulmonary hemorrhage, multiple emboli, and several other effects. Moreover, aminorex can cause idiopathic pulmonary hypertension, presenting another harmful and mostly lethal side-effect from cocaine cut with levamisole. In conclusion, levamisole determination should be performed in routine toxicological analysis in deaths due to cocaine use.
- Published
- 2017
15. Performances of Capillary Electrophoresis and HPLC Methods in HbA1cDetermination: Diagnostic Accuracy in HbS and HbD-Iran Variants' Presence
- Author
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Rossella Zenobi, Francesca Gabriela Martino, Stefano Pignalosa, Massimo Pieri, and Mariarita Dessì
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Chromatography ,business.industry ,Biochemistry (medical) ,Clinical Biochemistry ,Public Health, Environmental and Occupational Health ,Diagnostic accuracy ,Hematology ,High-performance liquid chromatography ,Surgery ,Medical Laboratory Technology ,Clinical therapy ,chemistry.chemical_compound ,Hba1c level ,Capillary electrophoresis ,chemistry ,medicine ,Immunology and Allergy ,In patient ,Hospital patients ,Glycated hemoglobin ,business - Abstract
Background Glycated hemoglobin (HbA1c) provides a useful estimate of mean glycemia in patients with diabetes and is directly related to risks for diabetes complications. The aim of this study is to compare a capillary electrophoresis method and two high-performance liquid chromatography (HPLC) cation-exchange analyzers (Variant II (Bio-Rad Laboratories, Inc., Hercules, CA) and G8 (Tosoh Biosciences, San Francisco, CA)) to identify the most reliable method in Hb variants' presence. Methods Measurements of HbA1c were carried out in blood samples from 200 Tor Vergata Hospital patients, using G8 Tosoh, and from 107 San Filippo Neri Hospital patients, using Variant II Bio-Rad methods. All samples were analyzed by Capillarys 2 Flex Piercing (FP; Sebia, Lisses, France). Results There was a good concordance between the results of capillary electrophoresis and HPLC methods (R2 = 0.99, P < 0.0001 for G8 HPLC; R2 = 0.99, P < 0.0001 for Variant II HPLC). During the study, we observed that some Hb variants, HbS and HbD-Iran, can alter the HbA1c level. Conclusions Since the HbA1c test is now recommended for diagnosing diabetes, and minimal variation of the concentration affects the clinical therapy, it is very important that the results are reliable and interference-free. Capillarys 2-FP analyzer is suitable for this purpose and sometimes it showed some advantages with respect to the HPLC analyzers tested, especially when Hb variants are present.
- Published
- 2014
16. Emoglobina glicata in base al genere: possibili valori di riferimento
- Author
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Rossella Zenobi, Francesca Gabriela Martino, Massimo Pieri, Francesca De Gregorio, Sergio Bernardini, Stefano Pignalosa, Mariarita Dessì, and Fabio Duranti
- Subjects
Medical Laboratory Technology ,Biochemistry (medical) - Abstract
Il diabete e diventato un’“epidemia globale”, come riportato dall’Organizzazione Mondiale della Sanita (OMS). L’emoglobina glicata (HbA1c), considerata una delle metodiche di elezione per la diagnosi di diabete, riflette la glicemia nel corso delle ultime 6–8 settimane. Valori elevati di HbA1c sono associati allo sviluppo di complicanze a lungo termine nel diabete sia di tipo 1 sia di tipo 2. Secondo le linee guida dell’OMS, valori di HbA1c ≥ 48 mmol/mol (6,5%) possono essere utilizzati per diagnosticare il diabete; attualmente questa raccomandazione non prevede differenze in base al genere. Lo scopo di questo studio e stato determinare valori di HbA1c in soggetti sani per verificare se vi sono differenze significative. Sono stati analizzati, mediante Capillarys 2-FP (Sebia, Lisses, Francia), 515 campioni di sangue ottenuti da donatori sani di etnia bianca caucasica afferenti all’Ospedale “San Filippo Neri” e al Policlinico Universitario “Tor Vergata”. I dati ottenuti hanno mostrato valori medi di HbA1c di 32,2 ± 4,0 mmol/mol per i maschi e 30 ± 3,8 mmol/mol per le femmine, con una differenza statisticamente significativa (p
- Published
- 2015
17. Free light chains nephelometric assay: human urine stability in different storage conditions
- Author
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Crisanti A, Massimo Pieri, Stefano Pignalosa, Dinallo, Mariarita Dessì, Casalino P, Rossella Z, and Sergio Bernardini
- Subjects
Chromatography ,free light chains ,Chemistry ,Settore BIO/12 ,Biochemistry (medical) ,Clinical Biochemistry ,General Medicine ,Urine ,030204 cardiovascular system & hematology ,Immunoglobulin light chain ,nephelometric assay ,urinary sample ,Humans ,Immunoglobulin Light Chains ,Nephelometry and Turbidimetry ,Specimen Handling ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis - Published
- 2015
18. Author's response to Letter to the Editor by N. Messaoudani, R. Djidjik and M. Ghaffor published in JNI 266 (2014) entitled 'Comments on CSF κFLC assay evaluation in assessing intrathecal synthesis'
- Author
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Rossella Zenobi, Stefano Pignalosa, Mariarita Dessì, and Massimo Pieri
- Subjects
030213 general clinical medicine ,Letter to the editor ,business.industry ,Immunology ,030209 endocrinology & metabolism ,Intrathecal ,03 medical and health sciences ,0302 clinical medicine ,Neurology ,Immunology and Allergy ,Medicine ,Neurology (clinical) ,business ,Nephelometry - Published
- 2017
19. Performances of capillary electrophoresis and HPLC methods in HbA1c determination: diagnostic accuracy in HbS and HbD-Iran variants' presence
- Author
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Mariarita, Dessi, Massimo, Pieri, Stefano, Pignalosa, Francesca Gabriela, Martino, and Rossella, Zenobi
- Subjects
Glycated Hemoglobin ,Male ,Hemoglobin, Sickle ,Statistics as Topic ,Diabetes Mellitus ,Electrophoresis, Capillary ,Humans ,Reproducibility of Results ,Female ,Original Articles ,Chromatography, High Pressure Liquid - Abstract
BACKGROUND: Glycated hemoglobin (HbA(1c)) provides a useful estimate of mean glycemia in patients with diabetes and is directly related to risks for diabetes complications. The aim of this study is to compare a capillary electrophoresis method and two high‐performance liquid chromatography (HPLC) cation‐exchange analyzers (Variant II (Bio‐Rad Laboratories, Inc., Hercules, CA) and G8 (Tosoh Biosciences, San Francisco, CA)) to identify the most reliable method in Hb variants' presence. METHODS: Measurements of HbA(1c) were carried out in blood samples from 200 Tor Vergata Hospital patients, using G8 Tosoh, and from 107 San Filippo Neri Hospital patients, using Variant II Bio‐Rad methods. All samples were analyzed by Capillarys 2 Flex Piercing (FP; Sebia, Lisses, France). RESULTS: There was a good concordance between the results of capillary electrophoresis and HPLC methods (R (2) = 0.99, P < 0.0001 for G8 HPLC; R (2) = 0.99, P < 0.0001 for Variant II HPLC). During the study, we observed that some Hb variants, HbS and HbD‐Iran, can alter the HbA(1c) level. CONCLUSIONS: Since the HbA(1c) test is now recommended for diagnosing diabetes, and minimal variation of the concentration affects the clinical therapy, it is very important that the results are reliable and interference‐free. Capillarys 2‐FP analyzer is suitable for this purpose and sometimes it showed some advantages with respect to the HPLC analyzers tested, especially when Hb variants are present.
- Published
- 2013
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