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8. In vitro and in silico analyses of Lippia alba (Verbenaceae) essential oil as an inhibitor of dengue virus and platelet activation.

Author

Silva-Trujillo, Lina, Ocazionez, Raquel E., Quintero-Rueda, Elizabeth, Stashenko, Elena E., Rondón-Villarreal, Paola, and Solarte-David, Victor A.

Subjects
DENGUE viruses, ESSENTIAL oils, BLOOD platelet activation, LIPPIA (Genus), VIRUS inhibitors
Abstract

Dengue virus (DENV) causes millions of infections each year, and there are currently no approved antivirals. Essential oils could serve as potential candidates for developing plant-based dengue treatments. Lippia alba (Mill.) N.E. Brown essential oil (LAEO) was selected to investigate the mode of antiviral action and its effect on activated platelets. Binding affinities of 20 LAEO compounds and platelet proteins were investigated through docking analyses. LAEO showed a potent virucidal effect (IC50, 2.1 to 5.1 μg/mL) against all DENV serotypes. LAEO reduced P-selectin (from 61% to 18%) and increased survival (from 71% to 97%) in DENV-2- and DENV NS1-stimulated human platelets. Sesquiterpene hydrocarbons showed the highest binding affinities (from −7.3 to −8.0 kcal/mol) with platelet innate immune receptors such as TLR2/1, TLR4/MD-2 and αIIbβ3 integrin. The data provide a first step towards defining the potential of LAEO as a candidate for developing phytotherapeutics for dengue. [ABSTRACT FROM AUTHOR]

Published
2024
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10. In vitroand in silicoanalyses of Lippia alba(Verbenaceae) essential oil as an inhibitor of dengue virus and platelet activation

Author

Silva-Trujillo, Lina, Ocazionez, Raquel E., Quintero-Rueda, Elizabeth, Stashenko, Elena E., Rondón-Villarreal, Paola, and Solarte-David, Victor A.

Abstract

ABSTRACTDengue virus (DENV) causes millions of infections each year, and there are currently no approved antivirals. Essential oils could serve as potential candidates for developing plant-based dengue treatments. Lippia alba(Mill.) N.E. Brown essential oil (LAEO) was selected to investigate the mode of antiviral action and its effect on activated platelets. Binding affinities of 20 LAEO compounds and platelet proteins were investigated through docking analyses. LAEO showed a potent virucidal effect (IC50, 2.1 to 5.1 μg/mL) against all DENV serotypes. LAEO reduced P-selectin (from 61% to 18%) and increased survival (from 71% to 97%) in DENV-2- and DENV NS1-stimulated human platelets. Sesquiterpene hydrocarbons showed the highest binding affinities (from −7.3 to −8.0 kcal/mol) with platelet innate immune receptors such as TLR2/1, TLR4/MD-2 and αIIbβ3 integrin. The data provide a first step towards defining the potential of LAEO as a candidate for developing phytotherapeutics for dengue.

Published
2024
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11. Enhancing Selectivity and Inhibitory Effects of Chemotherapy Drugs Against Myelogenous Leukemia Cells with Lippia alba Essential Oil Enriched in Citral.

Author

Quintero-García, Wendy Lorena, Espinel-Mesa, Denerieth Ximena, Moreno, Erika Marcela, Stashenko, Elena, Mesa-Arango, Ana Cecilia, and García, Liliana Torcoroma

Subjects
ACUTE myeloid leukemia, MYELOID leukemia, APOPTOSIS, ESSENTIAL oils, CELL death
Abstract

Acute myelogenous leukemia (AML) is one of the most lethal cancers, lacking a definitive curative therapy due to essential constraints related to the toxicity and efficacy of conventional treatments. This study explores the co-adjuvant potential of Lippia alba essential oils (EO) for enhancing the effectiveness and selectivity of two chemotherapy agents (cytarabine and clofarabine) against AML cells. EO derived from L. alba citral chemotype were produced using optimized and standardized environmental and extraction protocols. Rational fractionation techniques were employed to yield bioactive terpene-enriched fractions, guided by relative chemical composition and cytotoxic analysis. Pharmacological interactions were established between these fractions and cytarabine and clofarabine. The study comprehensively evaluated the cytotoxic, genotoxic, oxidative stress, and cell death phenotypes induced by therapies across AML (DA-3ER/GM/EVI1+) cells. The fraction rich in citral (F2) exhibited synergistic pharmacological interactions with the studied chemotherapies, intensifying their selective cytotoxic, genotoxic, and pro-oxidant effects. This shift favored transitioning from necrosis to a programmed cell death phenotype (apoptotic). The F2-clofarabine combination demonstrated remarkable synergistic anti-leukemic performance while preserving cell integrity in healthy cells. The observed selective antiproliferative effects may be attributed to the potential dual prooxidant/antioxidant behavior of citral in L. alba EO. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Actividad repelente e insecticida de cuatro aceites esenciales de plantas recolectadas en Chocó-Colombia contra Tribolium castaneum.

Author

Pino-Benítez, Nayive, Torralbo-Cabrera, Yuri P., and Stashenko, Elena E.

Subjects
RAIN forests, ESSENTIAL oils, REPELLENTS, PLANT collecting, PLANT species
Abstract

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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20. Photoprotective Activity of Ipomoea horsfalliae Flower Extract

Author

Sierra, Lady Johanna, Córdoba, Yuri, Mejía, Jesica Julieth, Rueda, Elizabeth Quintero, Ocazionez, Raquel Elvira, Avila-Acevedo, José Guillermo, García-Bores, Ana María, Espinosa-González, Adriana Montserrat, del Carmen Benítez-Flores, José, del Rosario González-Valle, María, Martínez, Jairo René, and Stashenko, Elena E.

Published
2020
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29. GC/MS Profile and Antifungal Activity of Zanthoxylum caribaeum Lam Essential Oil against Moniliophthora roreri Cif and Par, a Pathogen That Infects Theobroma cacao L Crops in the Tropics

Author

Fuentes-Estrada, Marcial, primary, Jiménez-González, Andrea, additional, Duarte, Diannefair, additional, Saavedra-Barrera, Rogerio, additional, Areche, Carlos, additional, Stashenko, Elena, additional, Pino Benítez, Nayive, additional, Bárcenas-Pérez, Daniela, additional, Cheel, José, additional, and García-Beltrán, Olimpo, additional

Published
2023
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42. Chemical Composition and In Vitro Antioxidant Activity of Salvia aratocensis (Lamiaceae) Essential Oils and Extracts

Author

Henríquez, Juan C., Duarte, Laura V., Sierra, Lady J., Fernández-Alonso, José L., Martínez, Jairo R., Stashenko, Elena E., Henríquez, Juan C., Duarte, Laura V., Sierra, Lady J., Fernández-Alonso, José L., Martínez, Jairo R., and Stashenko, Elena E.

Abstract

Salvia aratocensis (Lamiaceae) is an endemic shrub from the Chicamocha River Canyon in Santander (Colombia). Its essential oil (EO) was distilled from the aerial parts of the plant via steam distillation and microwave-assisted hydrodistillation and analyzed using GC/MS and GC/FID. Hydroethanolic extracts were isolated from dry plants before distillation and from the residual plant material after distillation. The extracts were characterized via UHPLC-ESI(+/−)-Orbitrap-HRMS. The S. aratocensis essential oil was rich in oxygenated sesquiterpenes (60–69%) and presented τ-cadinol (44–48%) and 1,10-di-epi-cubenol (21–24%) as its major components. The in vitro antioxidant activity of the EOs, measured via an ABTS+• assay, was 32–49 μmol Trolox® g−1 and that measured using the ORAC assay was 1520–1610 μmol Trolox® g−1. Ursolic acid (28.9–39.8 mg g−1) and luteolin-7-O-glucuronide (1.16–25.3 mg g−1) were the major S. aratocensis extract constituents. The antioxidant activity of the S. aratocensis extract, obtained from undistilled plant material, was higher (82 ± 4 μmol Trolox® g−1, ABTS+•; 1300 ± 14 μmol Trolox® g−1, ORAC) than that of the extracts obtained from the residual plant material (51–73 μmol Trolox® g−1, ABTS+•; 752–1205 μmol Trolox® g−1, ORAC). S. aratocensis EO and extract had higher ORAC antioxidant capacity than the reference substances butyl hydroxy toluene (98 μmol Trolox® g−1) and α-tocopherol (450 μmol Trolox® g−1). S. aratocensis EOs and ex

Published
2023

43. Mapping Protein Targets of Carnosol, a Molecule Identified in Rosmarinus officinalis: In Silico Docking Studies and Network Pharmacology

Author

Taboada-Alquerque, María, Pajaro-Valenzuela, Danilo, Caballero-Gallardo, Karina, Cifuentes, Alejandro, Ibáñez, Elena, Ahumedo-Monterrosa, Maicol, Stashenko, Elena E., Olivero-Verbel, Jesus, Taboada-Alquerque, María, Pajaro-Valenzuela, Danilo, Caballero-Gallardo, Karina, Cifuentes, Alejandro, Ibáñez, Elena, Ahumedo-Monterrosa, Maicol, Stashenko, Elena E., and Olivero-Verbel, Jesus

Abstract

Carnosol is a natural diterpene present in Rosmarinus officinalis L. (rosemary) with anti-tumor and anti-inflammatory properties. Despite its importance, the pharmacological mechanisms underlying the interactions between carnosol and human targets are still unclear. The goal was to identify plausible human target for carnosol and the network pharmacology. Rosemary was analyzed using HPLC-QTOF-MS/MS. Potential carnosol targets were identified using docking and a public database (CTD). Carnosol was screened against 708 human proteins using AutoDock Vina, and affinity values were used as prioritization criteria. The targets set was uploaded to WebGestalt to obtain Gene Ontology (GO) and KEGG pathway enrichment analysis. HPLC-QTOF-MS/MS analyses allowed the tentative annotation of nine chemicals, with carnosol being the most ionized. There were 53 plausible targets for carnosol, with 20 identified using virtual screening, including Hsp90α (−10.9 kcal/mol), AKR1C3 (−10.4 kcal/mol), and Hsp90β (−10.4 kcal/mol), and 33 identified from CTD. The potential targets for carnosol identified with PPI and molecular docking were HSP90AA1, MAPK1, MAPK3, CAT, JUN, AHR, and CASP3. GO terms and KEGG pathways analysis found that carnosol is closely related to infection (Chagas, influenza A, toxoplasmosis, and pertussis) and inflammation (IL-17 and TNF signaling pathway and Th-17 cell differentiation). These results demonstrated that carnosol may induce an immuno-inflammatory response.

Published
2023

44. Volatile metabolites, antioxidant and biological activities of Bursera simaruba(L.) Sarg. essential oil, from the tropical dry forest, Cesar, Colombian Caribbean Region

Author

Ravelo Martínez, Samuel Andrés, Rodríguez Melendez, Valeria Isabel, Rosero Rosero, Johanna Catalina, Stashenko, Elena, and Tafurt-García, Geovanna

Abstract

AbstractTropical Dry Forest (T-dF) is one of the most threatened ecosystems in the Neotropics, due to negative anthropogenic impacts on biodiversity and ecosystem services. Bursera simaruba(L.) Sarg. (indio desnudo, resbalamono), is among the species most reported for the T-dF of the Colombian Caribbean. In this work, the chemical composition and antioxidant and biological activities of B. simarubaessential oil (EO) were determined. EO chemical analysis from leaves and, petiole, and young branches bark (PYBB) of B. simarubawas carried out by Gas Chromatography coupled to Mass Spectrometry; polar and apolar columns were used for component separation. The biological activity of B. simarubaEO (PYBB) against Lasioderma serricorne(Coleoptera, Ptinidae) was determined by the preference area method. EO (PYBB) antioxidant capacity was determined by Oxygen Free Radical Absorbance Capacity (ORAC) assay. Reference antioxidant and standard terpenes were used for relative analysis of ORAC results. α-Terpinene (27.7% and 16.4%), p-cymene (22.8% and 44.0%), and g-terpinene (16.7% and 14.6%), were the main constituents in B. simarubaEOs (leaves and PYBB). EOs were represented by a high percentage of hydrocarbon monoterpenes (∼79.1% and ∼88.9%, leaves and PYBB); while hydrocarbon sesquiterpenes (∼3.1% and ∼4.6%, leaves and PYBB); contributed with a low percentage. B. simarubaEO (PYBB), showed attractant activity, at 0.4 μL.cm-2 (2h: -45%±7%; 4h: -50%±17%); in relation with Stay off, at 0.8 μL.cm-2, which was used as a positive control for repellent activity (2h: 66%±5%; 4h: 75%±21%). B. simarubaEO (PYBB) showed a very low antioxidant capacity (ORAC: 657.2±20.5 μmol Trolox/g sample), related to quercetin, used as reference antioxidant (50816.9±1559.1 μmol Trolox/g sample). However, EO ORAC was higher than the α-terpinene (68.0±10.8 μmol Trolox/g sample), γ-terpinene (51.2±2.0 μmol Trolox/g sample), p-cymene (105.6±6.4 μmol Trolox/g sample), used as standard terpenes. In conclusion, this is the first report on the chemical composition of the B. simarubaEO from Colombia, whose results differ from the previous ones from Jamaica and Guadeloupe. Chemical composition, reported here, with α-terpinene, γ-terpinene and p-cymene as principal compounds, could be used to establish a scientific support that explains some traditional uses. EO showed an attractive biological activity on L. serricorne.EO showed an antioxidant activity lowest quercetin (antioxidant reference), but higher that standard terpenes (α-terpinene, γ-terpinene and p-cymene).

Published
2024
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45. Photoprotective Activity of Ipomoea horsfalliaeFlower Extract

Author

Sierra, Lady Johanna, Córdoba, Yuri, Mejía, Jesica Julieth, Rueda, Elizabeth Quintero, Ocazionez, Raquel Elvira, Avila-Acevedo, José Guillermo, García-Bores, Ana María, Espinosa-González, Adriana Montserrat, del Carmen Benítez-Flores, José, del Rosario González-Valle, María, Martínez, Jairo René, and Stashenko, Elena E.

Abstract

In this work, we studied the potential photoprotective effect of Ipomoea horsfalliaeHook., Convolvulaceae, flower extract. Ipomoea horsfalliaeis a plant that grows in tropical and subtropical regions. I. horsfalliaeethanolic extracts were analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry. Dicaffeoylquinic acid, chlorogenic acid, scopoletin, glycosylated cyanidin, pelargonidin, and kaempferol were identified as major components of I. horsfalliaeflower extract. In vitrobiossays were used to evaluate cytotoxic and sensitizing effects of the extracts, and their photoprotective effect was evaluated in BALB/c mice. Morphological and histopathological observation of the skin tissues from mice suggested that UV-B-induced edema was significantly inhibited by treatment with I. horsfalliaeflower extract. It was not cytotoxic for both cancerous and normal cells, and no sensitizing effect was observed. I.horsfalliaeflower extract appears to be a good starting point for research programs leading to the development of natural skin care products.

Published
2024
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48. Essential oil of Cordia curassavica (Jacq) Roem. & Schult: in vitro and in silico evaluation of effects on dengue virus replication and cytokines production.

Author

Alejandra Velandia, Sindi, Quintero Rueda, Elizabeth, Rondon-Villarreal, Paola, Marcela Silva, Lina, Stashenko, Elena E., and Ocazionez, Raquel E.

Subjects
ESSENTIAL oils, VIRAL replication, VIRAL proteins, DENGUE viruses, DENGUE, CYTOKINES, MOLECULAR interactions, FENITROTHION
Abstract

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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