1. Multiomics links global surfactant dysregulation with airflow obstruction and emphysema in COPD
- Author
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Hristova, Ventzislava A, Watson, Alastair, Chaerkady, Raghothama, Glover, Matthew S, Ackland, Jodie, Angerman, Bastian, Belfield, Graham, Belvisi, Maria G, Burke, Hannah, Cellura, Doriana, Clark, Howard W, Etal, Damla, Freeman, Anna, Heinson, Ashley I, Hess, Sonja, Hühn, Michael, Hall, Emily, Mackay, Alex, Madsen, Jens, McCrae, Christopher, Muthas, Daniel, Novick, Steven, Ostridge, Kristoffer, Öberg, Lisa, Platt, Adam, Postle, Anthony D, Spalluto, C Mirella, Vaarala, Outi, Wang, Junmin, Staples, Karl J, Wilkinson, Tom MA, MICA II Study group, Watson, Alastair [0000-0002-6735-2567], Ackland, Jodie [0000-0003-3120-3620], Belvisi, Maria G [0000-0003-1652-4370], Cellura, Doriana [0000-0001-9403-2422], Clark, Howard W [0000-0001-7354-2028], Madsen, Jens [0000-0003-1664-7645], Öberg, Lisa [0000-0001-7127-1711], Spalluto, C Mirella [0000-0001-7273-0844], Staples, Karl J [0000-0003-3844-6457], and Apollo - University of Cambridge Repository
- Subjects
MICA II Study group - Abstract
RATIONALE: Pulmonary surfactant is vital for lung homeostasis as it reduces surface tension to prevent alveolar collapse and provides essential immune-regulatory and antipathogenic functions. Previous studies demonstrated dysregulation of some individual surfactant components in COPD. We investigated relationships between COPD disease measures and dysregulation of surfactant components to gain new insights into potential disease mechanisms. METHODS: Bronchoalveolar lavage proteome and lipidome were characterised in ex-smoking mild/moderate COPD subjects (n=26) and healthy ex-smoking (n=20) and never-smoking (n=16) controls using mass spectrometry. Serum surfactant protein analysis was performed. RESULTS: Total phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, surfactant protein (SP)-B, SP-A and SP-D concentrations were lower in COPD versus controls (log2 fold change (log2FC) -2.0, -2.2, -1.5, -0.5, -0.7 and -0.5 (adjusted p
- Published
- 2023