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Your search keyword '"Stanzin Choksket"' showing total 9 results
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9 results on '"Stanzin Choksket"'

1. Characterization and Antimicrobial Studies of Iturin-Like and Bogorol-Like Lipopeptides From Brevibacillus spp. Strains GI9 and SKDU10

Author

Shelley Sardul Singh, Deepika Sharma, Piyush Baindara, Stanzin Choksket, Harshvardhan, Santi M. Mandal, Vishakha Grover, and Suresh Korpole

Subjects
Brevibacillus, iturin, bogorol, ILL, BLL, lipopeptide, Microbiology, QR1-502
Abstract

Accession numbers for whole-genome sequence of Brevibacillus sp. strain GI9 and SKDU10 are CAGD01000001 to CAGD01000061 and LSSO00000000, respectively. Members of the genus Brevibacillus have been demonstrated to produce a variety of bioactive compounds including polyketides, lipopeptides and bacteriocins. Lipopeptides are non-ribosomally synthesized surface-active compounds with antimicrobial, antitumor, and immune-stimulatory activities. They usually exhibit strong antifungal and antibacterial activities and are considered as promising compounds in controlling fungal diseases. In this study, we have characterized two lipopeptides from Brevibacillus sp. strains GI9 and SKDU10. The corresponding lipopeptides were purified by reverse-phase high-performance liquid chromatography. Mass analysis and characterization by MALDI-TOF-MS (Matrix-assisted laser desorption ionization time-of-flight mass spectrometry) analysis revealed production of an iturin-like lipopeptide by strain GI9 and bogorol-like lipopeptide by strain SKDU10. Both lipopeptides exhibited broad spectrum antibacterial activity and inhibited the growth of various fungi. They showed minimum inhibitory concentration (MIC) values between 90 and 300 μg/ml against indicator strains of bacteria and drug-resistant Candida indicator strains. The lipopeptides did not show phytotoxic effect in seed germination experiments but caused hemolysis. Further, both lipopeptides inhibited the growth of fungi on fruits and vegetables in in vitro experiments, thereby exhibited potential use in biotechnological industry as effective biocontrol agents.

Published
2021
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5. Mycoplana

Author

Princy Hira, Utkarsh Sood, Stanzin Choksket, Krishna Gundawar, Suresh Korpole, Sukanya Lal, and Rup Lal

Published
2021

6. Lacrimispora defluvii PI-S10-B5A

Author

Urvashi, Krishna, Gundawar, Shikha, Sharma, Stanzin, Choksket, Mohini, Sharma, Vishakha, Grover, Prabhu B, Patil, and Suresh, Korpole

Subjects
DNA, Bacterial, Clostridium, Bacteria, Anaerobic, RNA, Ribosomal, 16S, Fatty Acids, Industrial Waste, Sequence Analysis, DNA, Phylogeny, Phospholipids, Bacterial Typing Techniques
Abstract

A bacterial strain was isolated from the waste slurry of an industrial effluent treatment plant near Patancheru, Hyderabad, India, and designated as PI-S10-B5A

Published
2022

7. Salinicoccus cyprini sp. nov., isolated from the gut of mirror carp, Cyprinus carpio var. specularis

Author

Stanzin Choksket, Amit Kumar Singh, Suresh Korpole, Ram Krishan Negi, Rup Lal, and Chandni Talwar

Subjects
chemistry.chemical_classification, biology, biology.animal_breed, General Medicine, biology.organism_classification, 16S ribosomal RNA, Microbiology, Amino acid, Cyprinus, Cell wall, Glycolipid, chemistry, Taxonomy (biology), Ecology, Evolution, Behavior and Systematics, Salinicoccus, Mirror carp
Abstract

A novel orange to pink coloured bacterial strain designated as CT19T was isolated from the gastrointestinal tract of mirror carp, Cyprinus carpio var. specularis (Lacepède, 1803) collected from the Gobind Sagar reservoir at village Lathiani, Una, Himachal Pradesh, India. Cells of the strain were found to be aerobic, Gram-stain-positive, non-motile and non-spore-forming coccoids. Based on the 16S rRNA gene sequence, the strain was closely related to Salinicoccus hispanicus J-82T (=DSM 5352T; 97.4 %), followed by S. sesuvii CC-SPL15-2T (=DSM 23267T; 96.4 %), S. amylolyticus JC304T (=KCTC 33661T; 95.6 %) and S. roseus DSM 5351T (95.4 %). Identity with all other members of the genus were T was assembled to 2.4 Mbp with a G+C content of 47.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain CT19T and S. hispanicus J-82T were found to be 85.9 and 31.3% respectively which is far below the threshold for species delineation. Iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0, C16 : 0 and anteiso-C17 : 0 were the major cellular fatty acids of strain CT19T. Major polar lipids were diphosphatidylglycerol, phosphatidylgylcerol and an unidentified glycolipid. Respiratory quinone system was composed of menaquinone-6 and major cell wall amino acid was l-lysine. Based on phylogenomic, physiological and biochemical characteristics, strain CT19T represents a novel species of the genus Salinicoccus for which the name Salinicoccus cyprini sp. nov. is proposed. The type strain is CT19T (=KCTC 43022T =CCM 8886T=MCC 3834T).

Published
2020

8. Sphingopyxis

Author

Helianthous Verma, Krishna Gundawar, Stanzin Choksket, Suresh Korpole, and Rup Lal

Published
2020

9. Paraclostridium dentum, a novel species with pathogenic features isolated from human dental plaque sample

Author

Stanzin Choksket, Urvashi, Ashish Jain, Vishakha Grover, Suresh Korpole, and Deepika Sharma

Subjects
Virulence Factors, Dental Plaque, Virulence, Biology, Dental plaque, Microbiology, Endospore, 03 medical and health sciences, RNA, Ribosomal, 16S, medicine, Gelatinase, Humans, Periodontitis, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Oxidase test, Clostridiales, Strain (chemistry), 030306 microbiology, Nucleic Acid Hybridization, Genomics, medicine.disease, 16S ribosomal RNA, Bacterial Typing Techniques, Infectious Diseases, Enzyme, chemistry, Genome, Bacterial
Abstract

A strictly anaerobic bacterial strain designated as SKVG24 was isolated from subgingival dental plaque samples of patients suffering from periodontitis. Cells were stained Gram-positive, rod shaped with endospore. The strain showed negative reaction to catalase and oxidase enzymes, but positive for gelatinase activity. Optimal growth was observed at 37 °C temperature and 7.0 pH. The 16S rRNA gene sequence BLAST analysis assigned strain SKVG24 to the genus Paraclostridium as it displayed 99.93% identity with P. benzoelyticum JC272T followed by P. bifermentans ATCC 638T (99.79%). However, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) of the whole genome sequence showed

Published
2020

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