39 results on '"Staněk D"'
Search Results
2. Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells
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Koberna, K., Staněk, D., Malínský, J., Eltsov, M., Pliss, A., Čtrnáctá, V., Cermanová, Š., and Raška, I.
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- 1999
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3. Novel SBF2 mutations and clinical spectrum of Charcot-Marie-Tooth neuropathy type 4B2
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Laššuthová, P., primary, Vill, K., additional, Erdem-Ozdamar, S., additional, Schröder, J.M., additional, Topaloglu, H., additional, Horvath, R., additional, Müller-Felber, W., additional, Bansagi, B., additional, Schlotter-Weigel, B., additional, Gläser, D., additional, Neupauerová, J., additional, Sedláčková, L., additional, Staněk, D., additional, Mazanec, R., additional, Weis, J., additional, Seeman, P., additional, and Senderek, J., additional
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- 2018
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4. Bone marrow edema and structural alterations in bone microarchitecture
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Zendeli, A., Muschitz, Ch., Kocijan, R., Stanek, D., Süss, D., and Resch, H.
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- 2012
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5. A 5-year-old boy with super-refractory status epilepticus and RANBP2 variant warranting life-saving hemispherotomy.
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Straka B, Koblížek M, Splítková B, Valkovičová R, Krsková L, Kalinová M, Vlčková M, Zámečník J, Laššuthová P, Sedláčková L, Staněk D, Maulisová A, Tichý M, Kynčl M, and Kršek P
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- Child, Child, Preschool, Humans, Male, Disease Progression, DNA, Midazolam, Brain Diseases, Drug Resistant Epilepsy genetics, Drug Resistant Epilepsy surgery, Epilepsy complications, Focal Cortical Dysplasia, Molecular Chaperones, Nuclear Pore Complex Proteins, Status Epilepticus genetics, Status Epilepticus surgery
- Abstract
Focal cortical dysplasia (FCD) represents the most common cause of drug-resistant epilepsy in adult and pediatric surgical series. However, genetic factors contributing to severe phenotypes of FCD remain unknown. We present a patient with an exceptionally rapid development of drug-resistant epilepsy evolving in super-refractory status epilepticus. We performed multiple clinical (serial EEG, MRI), biochemical (metabolic and immunological screening), genetic (WES from blood- and brain-derived DNA), and histopathological investigations. The patient presented 1 month after an uncomplicated varicella infection. MRI was negative, as well as other biochemical and immunological examinations. Whole-exome sequencing of blood-derived DNA detected a heterozygous paternally inherited variant NM_006267.4(RANBP2):c.5233A>G p.(Ile1745Val) (Chr2[GRCh37]:g.109382228A>G), a gene associated with a susceptibility to infection-induced acute necrotizing encephalopathy. No combination of anti-seizure medication led to a sustained seizure freedom and the patient warranted induction of propofol anesthesia with high-dose intravenous midazolam and continuous respiratory support that however failed to abort seizure activity. Brain biopsy revealed FCD type IIa; this finding led to the indication of an emergency right-sided hemispherotomy that rendered the patient temporarily seizure-free. Postsurgically, he remains on antiseizure medication and experiences rare nondisabling seizures. This report highlights a uniquely severe clinical course of FCD putatively modified by the RANBP2 variant. PLAIN LANGUAGE SUMMARY: We report a case summary of a patient who came to our attention for epilepsy that could not be controlled with medication. His clinical course progressed rapidly to life-threatening status epilepticus with other unusual neurological findings. Therefore, we decided to surgically remove a piece of brain tissue in order to clarify the diagnosis that showed features of a structural brain abnormality associated with severe epilepsy, the focal cortical dysplasia. Later, a genetic variant in a gene associated with another condition, was found, and we hypothesize that this genetic variant could have contributed to this severe clinical course of our patient., (© 2023 The Authors. Epilepsia Open published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.)
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- 2024
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6. Coilin and Cajal bodies.
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Staněk D
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- Coiled Bodies, Cell Nucleus
- Abstract
The nucleus of higher eukaryotes contains a number of structures that concentrate specific biomolecules and play distinct roles in nuclear metabolism. In recent years, the molecular mechanisms controlling their formation have been intensively studied. In this brief review, I focus on coilin and Cajal bodies. Coilin is a key scaffolding protein of Cajal bodies that is evolutionarily conserved in metazoans. Cajal bodies are thought to be one of the archetypal nuclear structures involved in the metabolism of several short non-coding nuclear RNAs. Yet surprisingly little is known about the structure and function of coilin, and a comprehensive model to explain the origin of Cajal bodies is also lacking. Here, I summarize recent results on Cajal bodies and coilin and discuss them in the context of the last three decades of research in this field.
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- 2023
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7. The SMN complex drives structural changes in human snRNAs to enable snRNP assembly.
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Pánek J, Roithová A, Radivojević N, Sýkora M, Prusty AB, Huston N, Wan H, Pyle AM, Fischer U, and Staněk D
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- Humans, SMN Complex Proteins metabolism, HeLa Cells, Ribonucleoproteins, Small Nuclear metabolism, snRNP Core Proteins genetics, RNA, Small Nuclear metabolism, RNA Precursors metabolism
- Abstract
Spliceosomal snRNPs are multicomponent particles that undergo a complex maturation pathway. Human Sm-class snRNAs are generated as 3'-end extended precursors, which are exported to the cytoplasm and assembled together with Sm proteins into core RNPs by the SMN complex. Here, we provide evidence that these pre-snRNA substrates contain compact, evolutionarily conserved secondary structures that overlap with the Sm binding site. These structural motifs in pre-snRNAs are predicted to interfere with Sm core assembly. We model structural rearrangements that lead to an open pre-snRNA conformation compatible with Sm protein interaction. The predicted rearrangement pathway is conserved in Metazoa and requires an external factor that initiates snRNA remodeling. We show that the essential helicase Gemin3, which is a component of the SMN complex, is crucial for snRNA structural rearrangements during snRNP maturation. The SMN complex thus facilitates ATP-driven structural changes in snRNAs that expose the Sm site and enable Sm protein binding., (© 2023. Springer Nature Limited.)
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- 2023
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8. Retinitis pigmentosa-associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule cells.
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Krausová M, Kreplová M, Banik P, Cvačková Z, Kubovčiak J, Modrák M, Zudová D, Lindovský J, Kubik-Zahorodna A, Pálková M, Kolář M, Procházka J, Sedláček R, and Staněk D
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- Animals, Mice, RNA, Circular, Mutation, Cerebellum, RNA-Binding Proteins genetics, Retinitis Pigmentosa
- Abstract
A subset of patients with retinitis pigmentosa (RP) carry mutations in several spliceosomal components including the PRPF8 protein. Here, we established two alleles of murine Prpf8 that genocopy or mimic aberrant PRPF8 found in RP patients-the substitution p.Tyr2334Asn and an extended protein variant p.Glu2331ValfsX15. Homozygous mice expressing the aberrant Prpf8 variants developed within the first 2 mo progressive atrophy of the cerebellum because of extensive granule cell loss, whereas other cerebellar cells remained unaffected. We further show that a subset of circRNAs were deregulated in the cerebellum of both Prpf8-RP mouse strains. To identify potential risk factors that sensitize the cerebellum for Prpf8 mutations, we monitored the expression of several splicing proteins during the first 8 wk. We observed down-regulation of all selected splicing proteins in the WT cerebellum, which coincided with neurodegeneration onset. The decrease in splicing protein expression was further pronounced in mouse strains expressing mutated Prpf8. Collectively, we propose a model where physiological reduction in spliceosomal components during postnatal tissue maturation sensitizes cells to the expression of aberrant Prpf8 and the subsequent deregulation of circRNAs triggers neuronal death., (© 2023 Krausová et al.)
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- 2023
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9. SART3 associates with a post-splicing complex.
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Klimešová K, Petržílková H, Bařinka C, and Staněk D
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- RNA, Small Nuclear genetics, RNA, Small Nuclear metabolism, Ribonucleoprotein, U4-U6 Small Nuclear genetics, Ribonucleoprotein, U4-U6 Small Nuclear metabolism, Ribonucleoprotein, U5 Small Nuclear genetics, Ribonucleoprotein, U5 Small Nuclear metabolism, Ribonucleoprotein, U2 Small Nuclear genetics, Ribonucleoprotein, U2 Small Nuclear metabolism, Ribonucleoproteins, Small Nuclear genetics, Ribonucleoproteins, Small Nuclear metabolism, RNA Splicing genetics, Spliceosomes genetics, Spliceosomes metabolism
- Abstract
SART3 is a multifunctional protein that acts in several steps of gene expression, including assembly and recycling of the spliceosomal U4/U6 small nuclear ribonucleoprotein particle (snRNP). In this work, we provide evidence that SART3 associates via its N-terminal HAT domain with the 12S U2 snRNP. Further analysis showed that SART3 associates with the post-splicing complex containing U2 and U5 snRNP components. In addition, we observed an interaction between SART3 and the RNA helicase DHX15, which disassembles post-splicing complexes. Based on our data, we propose a model that SART3 associates via its N-terminal HAT domain with the post-splicing complex, where it interacts with U6 snRNA to protect it and to initiate U6 snRNA recycling before a next round of splicing., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2023. Published by The Company of Biologists Ltd.)
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- 2023
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10. A point mutation in human coilin prevents Cajal body formation.
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Basello DA, Matera AG, and Staněk D
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- Animals, HeLa Cells, Humans, Mice, Mutation genetics, Nuclear Proteins genetics, Nuclear Proteins metabolism, Coiled Bodies genetics, Point Mutation genetics
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Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP-coilin construct that has a dominant-negative effect on Cajal body formation. We show that this coilin-K496E variant fails to rescue Cajal bodies in cells lacking endogenous coilin, whereas the wild-type construct restores Cajal bodies in mouse and human coilin-knockout cells. In cells containing endogenous coilin, both the wild-type and K496E variant proteins accumulate in Cajal bodies. However, high-level overexpression of coilin-K496E causes Cajal body disintegration. Thus, a mutation in the C-terminal region of human coilin can disrupt Cajal body assembly. Caution should be used when interpreting data from coilin plasmids that are derived from this variant (currently deposited at Addgene)., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2022. Published by The Company of Biologists Ltd.)
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- 2022
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11. Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity.
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Obuća M, Cvačková Z, Kubovčiak J, Kolář M, and Staněk D
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- Humans, Mutation, RNA Splice Sites, RNA Splicing, Spliceosomes metabolism, DEAD-box RNA Helicases genetics, RNA Splicing Factors genetics, Retinitis Pigmentosa genetics
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Retinitis pigmentosa (RP) is a hereditary disease affecting tens of thousands of people world-wide. Here we analyzed the effect of an amino acid substitution in the RNA helicase DHX38 (Prp16) causing RP. DHX38 has been proposed as the helicase important for the 2nd step of splicing. We showed that DHX38 associates with key splicing factors involved in both splicing steps but did not find any evidence that the RP mutations changes DHX38 interaction profile with the spliceosome. We further downregulated DHX38 and monitored changes in splicing. We observed only minor perturbations of general splicing but detected modulation of ~70 alternative splicing events. Next, we probed DHX38 function in splicing of retina specific genes and found that FSCN2 splicing is dependent on DHX38. In addition, RHO splicing was inhibited specifically by expression of DHX38 RP variant. Finally, we showed that overexpression of DHX38 promotes usage of canonical as well as cryptic 5' splice sites in HBB splicing reporter. Together, our data show that DHX38 is a splicing factor that promotes splicing of cryptic splice sites and regulate alternative splicing. We further provide evidence that the RP-linked substitution G332D modulates DHX38 splicing activity., Competing Interests: The authors have declared that no competing interests exist.
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- 2022
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12. Long non-coding RNAs and splicing.
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Staněk D
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- RNA Splicing, RNA, Long Noncoding genetics
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In this review I focus on the role of splicing in long non-coding RNA (lncRNA) life. First, I summarize differences between the splicing efficiency of protein-coding genes and lncRNAs and discuss why non-coding RNAs are spliced less efficiently. In the second half of the review, I speculate why splice sites are the most conserved sequences in lncRNAs and what additional roles could splicing play in lncRNA metabolism. I discuss the hypothesis that the splicing machinery can, besides its dominant role in intron removal and exon joining, protect cells from undesired transcripts., (© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
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- 2021
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13. Severe neurodevelopmental disorder with intractable seizures due to a novel SLC1A4 homozygous variant.
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Sedláčková L, Laššuthová P, Štěrbová K, Vlčková M, Kudr M, Buksakowska I, Staněk D, and Seeman P
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- Child, Homozygote, Humans, Male, Microcephaly pathology, Mutation, Neurodevelopmental Disorders pathology, Seizures pathology, Amino Acid Transport System ASC genetics, Microcephaly genetics, Neurodevelopmental Disorders genetics, Seizures genetics
- Abstract
Introduction: Biallelic variants in the SLC1A4 gene have been so far identified as a very rare cause of neurodevelopmental disorders with or without epilepsy and almost exclusively described in the Ashkenazi-Jewish population., Patients and Methods: Here we present Czech patient with microcephaly, severe global developmental delay and intractable seizures whose condition remained undiagnosed despite access to clinical experience and standard diagnostic methods including examination with an epilepsy targeted NGS gene panel., Results: Whole exome sequencing revealed a novel variant NM_003038.4:c.1370G > A p.(Arg457Gln) of the SLC1A4 gene in a homozygous state in the patient, and afterwards Sanger sequencing in both parents confirmed the biallelic origin of the variant. A variant in the same codon, but with a different amino acid exchange, was described previously in a patient that had a very similar phenotype, however, without epilepsy., Conclusion: Our data suggest that the SLC1A4 gene should be considered in the diagnosis of patients with severe, early onset neurodevelopmental impairment with epilepsy and encourage the analysis of SLC1A4 gene variants via targeted NGS gene panel or whole exome sequencing., (Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
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- 2021
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14. TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.
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Klimešová K, Vojáčková J, Radivojević N, Vandermoere F, Bertrand E, Verheggen C, and Staněk D
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- DNA Repair Enzymes metabolism, Down-Regulation, HeLa Cells, Humans, Nuclear Proteins metabolism, Peptide Elongation Factors, Protein Domains, Protein Interaction Domains and Motifs, RNA Splicing, RNA Splicing Factors metabolism, RNA-Binding Proteins metabolism, Recombinant Fusion Proteins, Ribonucleoproteins, Small Nuclear chemistry, Transcription Factors, Tumor Suppressor Proteins genetics, Ribonucleoprotein, U5 Small Nuclear chemistry, Ribonucleoprotein, U5 Small Nuclear metabolism, Ribonucleoproteins, Small Nuclear metabolism, Spliceosomes metabolism, Tumor Suppressor Proteins chemistry, Tumor Suppressor Proteins metabolism
- Abstract
U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leaves as a U5/PRPF19 post-splicing particle, which requires re-generation before the next round of splicing. Here, we show that a previously uncharacterized protein TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. We provide evidence that TSSC4 associates with U5 snRNP chaperones, U5 snRNP and the U5/PRPF19 particle. Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200. We also identified TSSC4 domains critical for the interaction with U5 snRNP and the PRPF19 complex, as well as for TSSC4 function in tri-snRNP assembly. TSSC4 emerges as a specific chaperone that acts in U5 snRNP de novo biogenesis as well as post-splicing recycling.
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- 2021
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15. The Cause of Hereditary Hearing Loss in GJB2 Heterozygotes-A Comprehensive Study of the GJB2 /DFNB1 Region.
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Safka Brozkova D, Uhrova Meszarosova A, Lassuthova P, Varga L, Staněk D, Borecká S, Laštůvková J, Čejnová V, Rašková D, Lhota F, Gašperíková D, and Seeman P
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- Gene Frequency, Heterozygote, Humans, Mutation, Connexin 26 genetics, Hearing Loss, Sensorineural genetics
- Abstract
Hearing loss is a genetically heterogeneous sensory defect, and the frequent causes are biallelic pathogenic variants in the GJB2 gene. However, patients carrying only one heterozygous pathogenic (monoallelic) GJB2 variant represent a long-lasting diagnostic problem. Interestingly, previous results showed that individuals with a heterozygous pathogenic GJB2 variant are two times more prevalent among those with hearing loss compared to normal-hearing individuals. This excess among patients led us to hypothesize that there could be another pathogenic variant in the GJB2 region/DFNB1 locus. A hitherto undiscovered variant could, in part, explain the cause of hearing loss in patients and would mean reclassifying them as patients with GJB2 biallelic pathogenic variants. In order to detect an unknown causal variant, we examined 28 patients using NGS with probes that continuously cover the 0.4 Mb in the DFNB1 region. An additional 49 patients were examined by WES to uncover only carriers. We did not reveal a second pathogenic variant in the DFNB1 region. However, in 19% of the WES-examined patients, the cause of hearing loss was found to be in genes other than the GJB2 . We present evidence to show that a substantial number of patients are carriers of the GJB2 pathogenic variant, albeit only by chance.
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- 2021
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16. DIS3L2 and LSm proteins are involved in the surveillance of Sm ring-deficient snRNAs.
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Roithová A, Feketová Z, Vaňáčová Š, and Staněk D
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- Base Sequence, Binding Sites, HeLa Cells, Humans, Organelles metabolism, Protein Binding, RNA Stability, SMN Complex Proteins metabolism, Exoribonucleases metabolism, Nucleic Acid Conformation, Proto-Oncogene Proteins metabolism, RNA Transport, RNA, Small Nuclear chemistry, RNA, Small Nuclear metabolism, RNA-Binding Proteins metabolism
- Abstract
Spliceosomal small nuclear ribonucleoprotein particles (snRNPs) undergo a complex maturation pathway containing multiple steps in the nucleus and in the cytoplasm. snRNP biogenesis is strictly proofread and several quality control checkpoints are placed along the pathway. Here, we analyzed the fate of small nuclear RNAs (snRNAs) that are unable to acquire a ring of Sm proteins. We showed that snRNAs lacking the Sm ring are unstable and accumulate in P-bodies in an LSm1-dependent manner. We further provide evidence that defective snRNAs without the Sm binding site are uridylated at the 3' end and associate with DIS3L2 3'→5' exoribonuclease and LSm proteins. Finally, inhibition of 5'→3' exoribonuclease XRN1 increases association of ΔSm snRNAs with DIS3L2, which indicates competition and compensation between these two degradation enzymes. Together, we provide evidence that defective snRNAs without the Sm ring are uridylated and degraded by alternative pathways involving either DIS3L2 or LSm proteins and XRN1., (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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- 2020
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17. Whole-Exome Sequencing in Czech Patients with Neurogenetic Diseases.
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Staněk D, Sedláčková L, Seeman P, Šafka Brožková D, and Laššuthová P
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- Adult, Alleles, Czech Republic, Female, Gene Frequency genetics, Genetic Variation genetics, Genomics methods, Genotype, High-Throughput Nucleotide Sequencing methods, Humans, Male, Phenotype, Sequence Analysis, DNA methods, Exome Sequencing methods, Databases, Genetic, Nervous System Diseases genetics, Neurodegenerative Diseases genetics
- Abstract
Aims: Genomic studies play a major role in variant observations between and within populations and in identifying causal relationships between genotypes and phenotypes. Analyses using databases such as gnomAD can provide insight into the frequencies of alleles in large populations. There have been reports that detail such frequencies for several countries and ethnic groups, but as yet, there are no such datasets for the Czech population. Patients and Methods: Whole-exome sequencing (WES) data from 222 individuals from the Czech Republic were analyzed by The Genome Analysis Toolkit best practices pipeline. These data were annotated with the ANNOVAR tool, and the allele frequencies were computed. Results: We developed a database that contains 300,111 variants in 17,512 genes. It is accessible through a simple web query available at prot2hg.com/variantbrowser. Gene-based analyses identified those genes that are most tolerant to variants in our population. Second, allele frequencies in our population were compared to the gnomAD database and groups of variants frequent in our population, but ultra-rare in gnomAD as a whole were identified. Conclusion: This tool should be useful for detecting local variants in the Czech population of patients with neurogenetic diseases.
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- 2020
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18. Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection.
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Roithová A and Staněk D
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- HeLa Cells, Humans, Microinjections methods, RNA, Small Nuclear metabolism
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Biogenesis of spliceosomal snRNAs is a complex process involving both nuclear and cytoplasmic phases and the last step occurs in a nuclear compartment called the Cajal body. However, sequences that direct snRNA localization into this subnuclear structure have not been known until recently. To determine sequences important for accumulation of snRNAs in Cajal bodies, we employed microinjection of fluorescently labelled snRNAs followed by their localization inside cells. First, we prepared snRNA deletion mutants, synthesized DNA templates for in vitro transcription and transcribed snRNAs in the presence of UTP coupled with Alexa488. Labelled snRNAs were mixed with 70 kDa-Dextran conjugated with TRITC, and microinjected to the nucleus or the cytoplasm of human HeLa cells. Cells were incubated for 1 h and fixed and the Cajal body marker coilin was visualized by indirect immunofluorescence, while snRNAs and dextran, which serves as a marker of nuclear or cytoplasmic injection, were observed directly using a fluorescence microscope. This method allows for efficient and rapid testing of how various sequences influence RNA localization inside cells. Here, we show the importance of the Sm-binding sequence for efficient localization of snRNAs into the Cajal body.
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- 2019
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19. UBTF Mutation Causes Complex Phenotype of Neurodegeneration and Severe Epilepsy in Childhood.
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Sedláčková L, Laššuthová P, Štěrbová K, Haberlová J, Vyhnálková E, Neupauerová J, Staněk D, Šedivá M, Kršek P, and Seeman P
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- Adolescent, Drug Resistant Epilepsy complications, Drug Resistant Epilepsy diagnostic imaging, Humans, Male, Neurodegenerative Diseases complications, Neurodegenerative Diseases diagnostic imaging, Drug Resistant Epilepsy genetics, Mutation genetics, Neurodegenerative Diseases genetics, Phenotype, Pol1 Transcription Initiation Complex Proteins genetics
- Abstract
Introduction: Neurodegenerative diseases of childhood present with progressive decline in cognitive, social, and motor function and are frequently associated with seizures in different stages of the disease. Here we report a patient with severe progressive neurodegeneration with drug-resistant epilepsy of unknown etiology from the age of 2 years., Methods and Results: Using whole exome sequencing, we found heterozygous missense de novo variant c.628G > A (p.Glu210Lys) in the UBTF gene. This variant was recently described as de novo in 11 patients with similar neurodegeneration characterized by developmental decline initially confined to motor development followed by language regression, appearance of an extrapyramidal movement disorder, and leading to severe intellectual disability. In 3 of the 11 patients described so far, seizures were also present., Conclusions: Our report expands the complex phenotype of neurodegeneration associated with the c.628G > A variant in the UBTF gene and helps to clarify the relation between this one single recurrent pathogenic variant described in this gene to date and its phenotype. The UBTF gene should be considered a novel candidate gene in neurodegeneration with or without epilepsy., Competing Interests: None of the authors has any conflicts of interest to disclose., (Georg Thieme Verlag KG Stuttgart · New York.)
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- 2019
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20. snRNP proteins in health and disease.
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Krausová M and Staněk D
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- Animals, Cryoelectron Microscopy, Humans, Mutation, Protein Conformation, Ribonucleoproteins, Small Nuclear chemistry, Ribonucleoproteins, Small Nuclear ultrastructure, Disease genetics, RNA Precursors genetics, RNA Splicing, Ribonucleoproteins, Small Nuclear genetics, Spliceosomes genetics
- Abstract
Split gene architecture of most human genes requires removal of intervening sequences by mRNA splicing that occurs on large multiprotein complexes called spliceosomes. Mutations compromising several spliceosomal components have been recorded in degenerative syndromes and haematological neoplasia, thereby highlighting the importance of accurate splicing execution in homeostasis of assorted adult tissues. Moreover, insufficient splicing underlies defective development of craniofacial skeleton and upper extremities. This review summarizes recent advances in the understanding of splicing factor function deduced from cryo-EM structures. We combine these data with the characterization of splicing factors implicated in hereditary or somatic disorders, with a focus on potential functional consequences the mutations may elicit in spliceosome assembly and/or performance. Given aberrant splicing or perturbations in splicing efficiency substantially underpin disease pathogenesis, profound understanding of the mis-splicing principles may open new therapeutic vistas. In three major sections dedicated to retinal dystrophies, hereditary acrofacial syndromes, and haematological malignancies, we delineate the noticeable variety of conditions associated with dysfunctional splicing and accentuate recurrent patterns in splicing defects., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
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- 2018
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21. Neonatal Onset of Epilepsy of Infancy with Migrating Focal Seizures Associated with a Novel GABRB3 Variant in Monozygotic Twins.
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Štěrbová K, Vlčková M, Klement P, Neupauerová J, Staněk D, Zůnová H, Seeman P, and Laššuthová P
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- Age of Onset, Diseases in Twins drug therapy, Diseases in Twins epidemiology, Epilepsy drug therapy, Epilepsy epidemiology, Female, Humans, Infant, Infant, Newborn, Diseases in Twins genetics, Epilepsy genetics, Mutation, Receptors, GABA-A genetics, Twins, Monozygotic genetics
- Abstract
Background: Recently, a study providing insight into GABRB3 mutational spectrum was published (Møller et al 2017). The authors report considerable pleiotropy even for single mutations and were not able to identify any genotype-phenotype correlations., Methods: The proband (twin B) was referred for massively parallel sequencing of epilepsy-related gene panel because of hypotonia and neonatal seizures. The revealed variant was confirmed with Sanger sequencing in the proband and the twin A, and both parents were tested for the presence of the variant., Results: We report a case of epilepsy of infancy with migrating focal seizures (EIMFS) of neonatal onset in monozygotic twins with a de novo novel GABRB3 variant p.Thr281Ala. The variant has a uniform presentation on an identical genomic background. In addition, early seizure-onset epilepsy associated with GABRB3 mutation has been until now described only for the p.Leu256Gln variant in the GABRB3 (Møller et al 2017, Myers et al 2016) located in the transmembrane domain just as the p.Thr281Ala variant described here., Conclusion: De novo GABRB3 mutations may cause neonatal-onset EIMFS with early-onset hypotonia, respiratory distress, and severe developmental delay., Competing Interests: None., (Georg Thieme Verlag KG Stuttgart · New York.)
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- 2018
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22. Detection rate of causal variants in severe childhood epilepsy is highest in patients with seizure onset within the first four weeks of life.
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Staněk D, Laššuthová P, Štěrbová K, Vlčková M, Neupauerová J, Krůtová M, and Seeman P
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- Chi-Square Distribution, Child, Child, Preschool, Female, Genetic Predisposition to Disease genetics, Humans, Infant, Male, Mutation, Spasms, Infantile genetics, Epilepsy genetics, Seizures genetics
- Abstract
Background: Epilepsy is a heterogeneous disease with a broad phenotypic spectrum and diverse genotypes. A significant proportion of epilepsies has a genetic aetiology. In our study, a custom designed gene panel with 112 genes known to be associated with epilepsies was used. In total, one hundred and fifty-one patients were tested (86 males / 65 females)., Results: In our cohort, the highest probability for the identification of the cause of the disease was for patients with a seizure onset within the first four weeks of life (61.9% clarification rate) - about two times more than other groups. The level of statistical significance was determined using a chi-square analysis. From 112 genes included in the panel, suspicious and rare variants were found in 53 genes (47.3%). Among the 151 probands included in the study we identified pathogenic variants in 39 patients (25.8%), likely pathogenic variants in three patients (2%), variants of uncertain significance in 40 patients (26.5%) and likely benign variants in 69 patients (45.7%)., Conclusion: Our report shows the utility of diagnostic genetic testing of severe childhood epilepsies in a large cohort of patients with a diagnostic rate of 25.8%. A gene panel can be considered as a method of choice for the detection of pathogenic variants within patients with unknown origin of early onset severe epilepsy.
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- 2018
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23. STRC Gene Mutations, Mainly Large Deletions, are a Very Important Cause of Early-Onset Hereditary Hearing Loss in the Czech Population.
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Marková SP, Brožková DŠ, Laššuthová P, Mészárosová A, Krůtová M, Neupauerová J, Rašková D, Trková M, Staněk D, and Seeman P
- Subjects
- Czech Republic, Humans, Intercellular Signaling Peptides and Proteins, Polymerase Chain Reaction, Sequence Deletion, Hearing Loss genetics, Membrane Proteins genetics, Mutation
- Abstract
Introduction: Hearing loss (HL) is the most common sensory deficit in humans. HL is an extremely heterogeneous condition presenting most frequently as a nonsyndromic (NS) condition inherited in an autosomal recessive (AR) pattern, termed DFNB. Mutations affecting the STRC gene cause DFNB type 16. Various types of mutations within the STRC gene have been reported from the U.S. and German populations, but no information about the relative contribution of STRC mutations to NSHL-AR among Czech patients is available., Methods and Patients: Two hundred and eighty-eight patients with prelingual NSHL, either sporadic (n = 207) or AR (n = 81), who had been previously tested negative for the mutations affecting the GJB2 gene, were included in the study. These patients were tested for STRC mutations by a quantitative comparative fluorescent polymerase chain reaction (QF-PCR) assay. In addition, 31 of the 81 NSHL-AR patients were analyzed by massively parallel sequencing using one of two different gene panels: 23 patients were analyzed by multiplex-ligation probe amplification (MLPA); and 9 patients by SNP microarrays., Results: Causal mutations affecting the STRC gene (including copy number variations [CNVs] and point mutations) were found in 5.5% of all patients and 13.6% of the 81 patients in the subgroup with NSHL-AR., Conclusion: Our results provide strong evidence that STRC gene mutations are an important cause of NSHL-AR in Czech HL patients and are probably the second most common cause of DFNB. Large CNVs were more frequent than point mutations and it is reasonable to test them first by a QF-PCR method-a simple, accessible, and efficient tool for STRC CNV detection, which can be combined by MLPA.
- Published
- 2018
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- View/download PDF
24. Disease-Causing Variants in the ATL1 Gene Are a Rare Cause of Hereditary Spastic Paraplegia among Czech Patients.
- Author
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Mészárosová AU, Grečmalová D, Brázdilová M, Dvořáčková N, Kalina Z, Čermáková M, Vávrová D, Smetanová I, Staněk D, and Seeman P
- Subjects
- Adolescent, Child, Czech Republic, DNA Mutational Analysis, Exons, Female, Humans, Infant, Introns, Male, Middle Aged, Mutation, Missense, Pedigree, Young Adult, GTP-Binding Proteins genetics, Membrane Proteins genetics, Spastic Paraplegia, Hereditary genetics
- Abstract
Variants in the ATL1 gene have been repeatedly described as the second most frequent cause of hereditary spastic paraplegia (HSP), a motor neuron disease manifested by progressive lower limb spasticity and weakness. Variants in ATL1 have been described mainly in patients with early onset HSP. We performed Sanger sequencing of all coding exons and adjacent intron regions of the ALT1 gene in 111 Czech patients with pure form of HSP and additional Multiplex-Ligation Probe Analysis (MLPA) testing targeting the ATL1 gene in 56 of them. All patients except seven were previously tested by Sanger sequencing of the SPAST gene with negative results. ATL1 diagnostic testing revealed only five missense variants in the ATL1 gene. Four of them are novel, but we suppose only two of them to be pathogenic and causal. The remaining variants are assumed to be benign. MLPA testing in 56 of sequence variant negative patients revealed no gross deletion in the ATL1 gene. Variants in the ATL1 gene are more frequent in patients with early onset HSP, but in general the occurrence of pathogenic variants in the ATL1 gene is low in our cohort, less than 4.5% and less than 11.1% in patients with onset before the age of ten. Variants in the ATL1 gene are a less frequent cause of HSP among Czech patients than has been previously reported among other populations., (© 2017 John Wiley & Sons Ltd/University College London.)
- Published
- 2017
- Full Text
- View/download PDF
25. Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.
- Author
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Malinová A, Cvačková Z, Matějů D, Hořejší Z, Abéza C, Vandermoere F, Bertrand E, Staněk D, and Verheggen C
- Subjects
- Calcium-Binding Proteins metabolism, HeLa Cells, Humans, Multiprotein Complexes, Mutation, Peptide Elongation Factors genetics, Peptide Elongation Factors metabolism, Protein Binding, Protein Interaction Domains and Motifs, Protein Stability, Proteomics methods, RNA Interference, RNA Precursors genetics, RNA, Messenger genetics, RNA-Binding Proteins genetics, Retinitis Pigmentosa genetics, Retinitis Pigmentosa metabolism, Ribonucleoprotein, U1 Small Nuclear metabolism, Ribonucleoprotein, U4-U6 Small Nuclear metabolism, Ribonucleoprotein, U5 Small Nuclear genetics, Transfection, HSP90 Heat-Shock Proteins metabolism, RNA Precursors metabolism, RNA Splicing, RNA, Messenger metabolism, RNA-Binding Proteins metabolism, Ribonucleoprotein, U5 Small Nuclear metabolism
- Abstract
Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the HSP90/R2TP complex, its ZNHIT2 cofactor, and additional proteins. HSP90 and R2TP bind unassembled U5 proteins in the cytoplasm, stabilize them, and promote the formation of the U5 snRNP. We further found that PRPF8 mutants causing Retinitis pigmentosa assemble less efficiently with the U5 snRNP and bind more strongly to R2TP, with one mutant retained in the cytoplasm in an R2TP-dependent manner. We propose that the HSP90/R2TP chaperone system promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8. The proteomics data further reveal new interactions between R2TP and the tuberous sclerosis complex (TSC), pointing to a potential link between growth signals and the assembly of key cellular machines., (© 2017 Malinová et al.)
- Published
- 2017
- Full Text
- View/download PDF
26. Cajal bodies and snRNPs - friends with benefits.
- Author
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Staněk D
- Subjects
- Animals, Humans, Protein Binding, RNA Processing, Post-Transcriptional, Ribonucleoproteins, Small Nuclear genetics, Spliceosomes, Transcription, Genetic, Coiled Bodies metabolism, Ribonucleoproteins, Small Nuclear metabolism
- Abstract
Spliceosomal snRNPs are complex particles that proceed through a fascinating maturation pathway. Several steps of this pathway are closely linked to nuclear non-membrane structures called Cajal bodies. In this review, I summarize the last 20 y of research in this field. I primarily focus on snRNP biogenesis, specifically on the steps that involve Cajal bodies. I also evaluate the contribution of the Cajal body in snRNP quality control and discuss the role of snRNPs in Cajal body formation.
- Published
- 2017
- Full Text
- View/download PDF
27. Nuclear bodies: news insights into structure and function.
- Author
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Staněk D and Fox AH
- Subjects
- Animals, Cell Nucleus metabolism, Humans, Intranuclear Inclusion Bodies pathology, Nuclear Proteins metabolism, RNA, Untranslated metabolism, Intranuclear Inclusion Bodies chemistry, Intranuclear Inclusion Bodies metabolism
- Abstract
The cell nucleus contains a number of different dynamic bodies that are variously composed of proteins and generally, but not always, specific RNA molecules. Recent studies have revealed new understanding about nuclear body formation and function in different aspects of nuclear metabolism. Here, we focus on findings describing the role of nuclear bodies in the biogenesis of specific ribonucleoprotein complexes, processing of key mRNAs, and subnuclear sequestration of protein factors. We highlight how nuclear bodies are involved in stress responses, innate immunity and tumorigenesis. We further review organization of nuclear bodies and principles that govern their assembly, highlighting the pivotal role of scaffolding noncoding RNAs, and liquid-liquid phase separation, which are transforming our picture of nuclear body formation., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
28. Mutations in spliceosomal proteins and retina degeneration.
- Author
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Růžičková Š and Staněk D
- Subjects
- Animals, Humans, Introns, Mice, Mutation, Missense, RNA Precursors metabolism, RNA Splicing, RNA Splicing Factors metabolism, Rats, Ribonucleoproteins, Small Nuclear metabolism, RNA Precursors genetics, RNA Splicing Factors genetics, Retinitis Pigmentosa genetics, Ribonucleoproteins, Small Nuclear genetics, Spliceosomes genetics
- Abstract
A majority of human genes contain non-coding intervening sequences - introns that must be precisely excised from the pre-mRNA molecule. This event requires the coordinated action of five major small nuclear ribonucleoprotein particles (snRNPs) along with additional non-snRNP splicing proteins. Introns must be removed with nucleotidal precision, since even a single nucleotide mistake would result in a reading frame shift and production of a non-functional protein. Numerous human inherited diseases are caused by mutations that affect splicing, including mutations in proteins which are directly involved in splicing catalysis. One of the most common hereditary diseases associated with mutations in core splicing proteins is retinitis pigmentosa (RP). So far, mutations in more than 70 genes have been connected to RP. While the majority of mutated genes are expressed specifically in the retina, eight target genes encode for ubiquitous core snRNP proteins (Prpf3, Prpf4, Prpf6, Prpf8, Prpf31, and SNRNP200/Brr2) and splicing factors (RP9 and DHX38). Why mutations in spliceosomal proteins, which are essential in nearly every cell in the body, causes a disease that displays such a tissue-specific phenotype is currently a mystery. In this review, we recapitulate snRNP functions, summarize the missense mutations which are found in spliceosomal proteins as well as their impact on protein functions and discuss specific models which may explain why the retina is sensitive to these mutations.
- Published
- 2017
- Full Text
- View/download PDF
29. TALE-directed local modulation of H3K9 methylation shapes exon recognition.
- Author
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Bieberstein NI, Kozáková E, Huranová M, Thakur PK, Krchňáková Z, Krausová M, Carrillo Oesterreich F, and Staněk D
- Subjects
- Alternative Splicing genetics, Chromatin metabolism, Fibronectins genetics, HeLa Cells, Humans, Methylation, Transcription, Genetic, Exons genetics, Histones metabolism, Lysine metabolism, Transcription Activator-Like Effectors metabolism
- Abstract
In search for the function of local chromatin environment on pre-mRNA processing we established a new tool, which allows for the modification of chromatin using a targeted approach. Using Transcription Activator-Like Effector domains fused to histone modifying enzymes (TALE-HME), we show locally restricted alteration of histone methylation modulates the splicing of target exons. We provide evidence that a local increase in H3K9 di- and trimethylation promotes inclusion of the target alternative exon, while demethylation by JMJD2D leads to exon skipping. We further demonstrate that H3K9me3 is localized on internal exons genome-wide suggesting a general role in splicing. Consistently, targeting of the H3K9 demethylase to a weak constitutive exon reduced co-transcriptional splicing. Together our data show H3K9 methylation within the gene body is a factor influencing recognition of both constitutive and alternative exons.
- Published
- 2016
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- View/download PDF
30. SART3-Dependent Accumulation of Incomplete Spliceosomal snRNPs in Cajal Bodies.
- Author
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Novotný I, Malinová A, Stejskalová E, Matějů D, Klimešová K, Roithová A, Švéda M, Knejzlík Z, and Staněk D
- Abstract
Cajal bodies (CBs) are evolutionarily conserved nuclear structures involved in the metabolism of spliceosomal small nuclear ribonucleoprotein particles (snRNPs). CBs are not present in all cell types, and the trigger for their formation is not yet known. Here, we depleted cells of factors required for the final steps of snRNP assembly and assayed for the presence of stalled intermediates in CBs. We show that depletion induces formation of CBs in cells that normally lack these nuclear compartments, suggesting that CB nucleation is triggered by an imbalance in snRNP assembly. Accumulation of stalled intermediates in CBs depends on the di-snRNP assembly factor SART3. SART3 is required for both the induction of CB formation as well as the tethering of incomplete snRNPs to coilin, the CB scaffolding protein. We propose a model wherein SART3 monitors tri-snRNP assembly and sequesters incomplete particles in CBs, thereby allowing cells to maintain a homeostatic balance of mature snRNPs in the nucleoplasm., (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
31. Coilin: The first 25 years.
- Author
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Machyna M, Neugebauer KM, and Staněk D
- Subjects
- Animals, History, 20th Century, History, 21st Century, Humans, Nuclear Proteins chemistry, Nuclear Proteins history, Coiled Bodies metabolism, Nuclear Proteins metabolism, Protein Processing, Post-Translational
- Abstract
Initially identified as a marker of coiled bodies (now Cajal bodies or CBs), the protein coilin was discovered a quarter of century ago. Coilin is now known to scaffold the CB, but its structure and function are poorly understood. Nearly devoid of predicted structural motifs, coilin has numerous reported molecular interactions that must underlie its role in the formation and function of CBs. In this review, we summarize what we have learned in the past 25 years about coilin's structure, post-transcriptional modifications, and interactions with RNA and proteins. We show that genes with homology to human coilin are found in primitive metazoans and comment on differences among model organisms. Coilin's function in Cajal body formation and RNP metabolism will be discussed in the light of these developments.
- Published
- 2015
- Full Text
- View/download PDF
32. The splicing factor U1-70K interacts with the SMN complex and is required for nuclear gem integrity.
- Author
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Stejskalová E and Staněk D
- Subjects
- Binding Sites, Cell Nucleus chemistry, Cell Nucleus genetics, Cell Nucleus metabolism, Gemini of Coiled Bodies chemistry, HeLa Cells, Humans, Protein Binding, Protein Transport, RNA Splicing, Ribonucleoprotein, U1 Small Nuclear chemistry, Ribonucleoprotein, U1 Small Nuclear genetics, SMN Complex Proteins genetics, Gemini of Coiled Bodies metabolism, Ribonucleoprotein, U1 Small Nuclear metabolism, SMN Complex Proteins metabolism
- Abstract
The nuclear SMN complex localizes to specific structures called nuclear gems. The loss of gems is a cellular marker for several neurodegenerative diseases. Here, we identify that the U1-snRNP-specific protein U1-70K localizes to nuclear gems, and we show that U1-70K is necessary for gem integrity. Furthermore, we show that the interaction between U1-70K and the SMN complex is RNA independent, and we map the SMN complex binding site to the unstructured N-terminal tail of U1-70K. Consistent with these results, the expression of the U1-70K N-terminal tail rescues gem formation. These findings show that U1-70K is an SMN-complex-associating protein, and they suggest a new function for U1-70K in the formation of nuclear gems., (© 2014. Published by The Company of Biologists Ltd.)
- Published
- 2014
- Full Text
- View/download PDF
33. Retinitis pigmentosa mutations of SNRNP200 enhance cryptic splice-site recognition.
- Author
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Cvačková Z, Matějů D, and Staněk D
- Subjects
- Alternative Splicing, Cloning, Molecular, Genes, Reporter, HeLa Cells, Humans, RNA Helicases genetics, RNA Precursors genetics, RNA Precursors metabolism, Spliceosomes, beta-Globins genetics, beta-Globins metabolism, Mutation, RNA Splice Sites genetics, Retinitis Pigmentosa genetics, Ribonucleoproteins, Small Nuclear genetics
- Abstract
Mutations in SNRP200 gene cause autosomal-dominant retinal disorder retinitis pigmentosa (RP). The protein product of SNRNP200 is BRR2, a DExD/H box RNA helicase crucial for pre-mRNA splicing. In this study, we prepared p.S1087L and p.R1090L mutations of human BRR2 using bacterial artificial chromosome recombineering and stably expressed them in human cell culture. Mutations in BRR2 did not compromise snRNP assembly and both mutants were incorporated into the spliceosome just as the wild-type (wt) protein. Surprisingly, cells expressing RP mutants exhibited increased splicing efficiency of the LDHA gene. Next, we found that depletion of endogenous BRR2 enhanced usage of a β-globin cryptic splice site while splicing at the correct splice site was inhibited. Proper splicing of optimal and cryptic splice sites was restored in cells expressing BRR2-wt but not in cells expressing RP mutants. Taken together, our data suggest that BRR2 is an important factor in 5'-splice-site recognition and that the RP-linked mutations c.3260C>T (p.S1087L) and c.3269G>T (p.R1090L) affect this BRR2 function., (© 2013 WILEY PERIODICALS, INC.)
- Published
- 2014
- Full Text
- View/download PDF
34. CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation.
- Author
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Dušková E, Hnilicová J, and Staněk D
- Subjects
- Acetylation, E1A-Associated p300 Protein genetics, Fibronectins metabolism, Gene Knockdown Techniques, Genes, Reporter, HeLa Cells, Humans, Integrases genetics, Promoter Regions, Genetic, Alternative Splicing, E1A-Associated p300 Protein metabolism, Fibronectins genetics, Histones metabolism, RNA, Messenger metabolism
- Abstract
Histone acetylation modulates alternative splicing of several hundred genes. Here, we tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon. p300 associates with CRE sites in the promoter via the CREB transcription factor. We created mini-gene reporters driven by an artificial promoter containing CRE sites. Both deletion and mutation of the CRE site affected EDB alternative splicing in the same manner as p300 knockdown. Next we showed that p300 controls histone H4 acetylation along the FN1 gene. Consistently, p300 depletion and CRE deletion/mutation both reduced histone H4 acetylation on mini-gene reporters. Finally, we provide evidence that the effect of CRE inactivation on H4 acetylation and alternative splicing is counteracted by the inhibition of histone deacetylases. Together, these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing.
- Published
- 2014
- Full Text
- View/download PDF
35. The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing.
- Author
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Hnilicová J, Hozeifi S, Stejskalová E, Dušková E, Poser I, Humpolíčková J, Hof M, and Staněk D
- Subjects
- Alternative Splicing, HeLa Cells, Histones metabolism, Humans, Microscopy, Video, Promoter Regions, Genetic, Protein Binding, Protein Serine-Threonine Kinases metabolism, Protein Structure, Tertiary, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Signal Transduction, Transcription Factors, Transcription, Genetic, Chromatin metabolism, Gene Expression Regulation, Genome, Human, Histones genetics, Protein Serine-Threonine Kinases genetics
- Abstract
Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome-wide screen, we identify 1450 genes whose transcription is regulated by Brd2. In addition, almost 290 genes change their alternative splicing pattern upon Brd2 depletion. Brd2 is specifically localized at promoters of target genes, and our data show that Brd2 interaction with chromatin cannot be explained solely by histone acetylation. Using coimmunoprecipitation and live-cell imaging, we show that the C-terminal part is crucial for Brd2 association with chromatin. Live-cell microscopy also allows us to map the average binding time of Brd2 to chromatin and quantify the contributions of individual Brd2 domains to the interaction with chromatin. Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin.
- Published
- 2013
- Full Text
- View/download PDF
36. Probing nucleic acid interactions and pre-mRNA splicing by Förster Resonance Energy Transfer (FRET) microscopy.
- Author
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Simková E and Staněk D
- Subjects
- Alternative Splicing, Molecular Conformation, Nucleic Acids chemistry, Nucleoproteins metabolism, Protein Binding, RNA Precursors chemistry, Spliceosomes genetics, Spliceosomes metabolism, Fluorescence Resonance Energy Transfer, Microscopy, Fluorescence, Nucleic Acids genetics, Nucleic Acids metabolism, RNA Precursors genetics, RNA Precursors metabolism, RNA Splicing
- Abstract
Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.
- Published
- 2012
- Full Text
- View/download PDF
37. Where splicing joins chromatin.
- Author
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Hnilicová J and Staněk D
- Subjects
- Animals, Chromatin metabolism, Humans, Nucleoproteins metabolism, RNA Precursors genetics, Transcription Factors metabolism, Transcription, Genetic genetics, Chromatin genetics, RNA Splicing
- Abstract
There are numerous data suggesting that two key steps in gene expression-transcription and splicing influence each other closely. For a long time it was known that chromatin modifications regulate transcription, but only recently it was shown that chromatin and histone modifications play a significant role in pre-mRNA splicing. Here we summarize interactions between splicing machinery and chromatin and discuss their potential functional significance. We focus mainly on histone acetylation and methylation and potential mechanisms of their role in splicing. It seems that whereas histone acetylation acts mainly by alterating the transcription rate, histone methylation can also influence splicing directly by recruiting various splicing components.
- Published
- 2011
- Full Text
- View/download PDF
38. In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies.
- Author
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Novotný I, Blažíková M, Staněk D, Herman P, and Malinsky J
- Subjects
- Antigens, Neoplasm genetics, Cell Line, Tumor, Cell Nucleus genetics, Cell Nucleus metabolism, Coiled Bodies genetics, HeLa Cells, Humans, Kinetics, Protein Binding genetics, RNA Helicases genetics, RNA Helicases metabolism, RNA Precursors genetics, RNA Precursors metabolism, RNA Splicing genetics, RNA-Binding Proteins genetics, Ribonucleoprotein, U4-U6 Small Nuclear genetics, Ribonucleoprotein, U5 Small Nuclear genetics, Ribonucleoprotein, U5 Small Nuclear metabolism, Ribonucleoproteins, Small Nuclear genetics, Spliceosomes genetics, Spliceosomes metabolism, Antigens, Neoplasm metabolism, Coiled Bodies metabolism, Models, Molecular, Nuclear Proteins metabolism, RNA-Binding Proteins metabolism, Ribonucleoprotein, U4-U6 Small Nuclear metabolism, Ribonucleoproteins, Small Nuclear metabolism
- Abstract
The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that ∼230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Förster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs.
- Published
- 2011
- Full Text
- View/download PDF
39. Histone deacetylase activity modulates alternative splicing.
- Author
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Hnilicová J, Hozeifi S, Dušková E, Icha J, Tománková T, and Staněk D
- Subjects
- Alternative Splicing drug effects, Alternative Splicing genetics, Animals, Cells, Cultured, Gene Expression Profiling, Gene Expression Regulation drug effects, HeLa Cells, Histone Deacetylase 1 genetics, Histone Deacetylase 1 physiology, Histone Deacetylase Inhibitors pharmacology, Histone Deacetylases genetics, Humans, Mice, Microarray Analysis, Nuclear Proteins genetics, RNA-Binding Proteins genetics, Serine-Arginine Splicing Factors, Transfection, Alternative Splicing physiology, Histone Deacetylases metabolism, Histone Deacetylases physiology
- Abstract
There is increasing evidence to suggest that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ∼700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition induced histone H4 acetylation and increased RNA Polymerase II (Pol II) processivity along an alternatively spliced element. In addition, HDAC inhibition reduced co-transcriptional association of the splicing regulator SRp40 with the target fibronectin exon. We further showed that the depletion of HDAC1 had similar effect on fibronectin alternative splicing as global HDAC inhibition. Importantly, this effect was reversed upon expression of mouse HDAC1 but not a catalytically inactive mutant. These results provide a molecular insight into a complex modulation of splicing by HDACs and chromatin modifications.
- Published
- 2011
- Full Text
- View/download PDF
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